vavilovВавиловский журнал генетики и селекцииVavilov Journal of Genetics and Breeding2500-3259Institute of Cytology and Genetics of Siberian Branch of the RAS10.18699/VJ17.275vavilov-1144Research ArticleМЕДИЦИНСКАЯ ГЕНЕТИКА, НЕЙРОГЕНЕТИКА, ИММУНОГЕНЕТИКА, БИОМЕДИЦИНАPOPULATION GENETICSГенетический полиморфизм и сопутствующие факторы риска ишемического инсульта в монгольской популяции КитаяGenetic polymorphisms and related risk factors of ischemic stroke in a Mongolian population in ChinaУЧ.WuQ.

Tongliao

УХ.WuH.

Tongliao

ОрловЮ. Л.OrlovYu. L.

Новосибирск

Novosibirsk

orlov@bionet.nsc.ruГегентанаГ.GegentanaG.

Tongliao

ХуоВ.HuoW.

Tongliao

БрагинА. О.BraginA. O.

Novosibirsk

УН.WuN.

Tongliao;

Virginia

СуялатуС.SuyalatuS.

Tongliao

ЖаоФ.ZhaoF.

Tongliao

ЖаоЖ.ZhaoJ.

Tongliao

ТабихановаЛ. Э.TabikhanovaL. E.

Novosibirsk

ЧенМ.ChenM.

Department of Bioinformatics, College of Life Sciences,

Hangzhou

mchen@zju.edu.cnБайХ.BaiH.

Tongliao

hh_bai@163.comНациональный университет Внутренней Монголии, ТунляоКитайInner Mongolia University for the NationalitiesChinaФедеральный исследовательский центр Институт цитологии и генетики Сибирского отделения Российской академии наук; Новосибирский государственный университетРоссияInstitute of Cytology and Genetics SB RAS; Novosibirsk State UniversityRussian FederationФедеральный исследовательский центр Институт цитологии и генетики Сибирского отделения Российской академии наук, НовосибирскРоссияInstitute of Cytology and Genetics SB RASRussian FederationНациональный университет Внутренней Монголии, Тунляо Колледж Северной Вирджинии, ВирджинияКитайInner Mongolia University for the Nationalities; Northern Virginia Community CollegeChinaОтдел биоинформатики, Колледж естественных наук, Университет Чжецзян, ХанчжоуКитайZhejiang UniversityChina201701112017215581587Copyright © У Ч., У Х., Орлов Ю.Л., Гегентана Г., Хуо В., Брагин А.О., У Н., Суялату С., Жао Ф., Жао Ж., Табиханова Л.Э., Чен М., Бай Х., 20172017У Ч., У Х., Орлов Ю.Л., Гегентана Г., Хуо В., Брагин А.О., У Н., Суялату С., Жао Ф., Жао Ж., Табиханова Л.Э., Чен М., Бай Х.Wu Q., Wu H., Orlov Y.L., Gegentana G., Huo W., Bragin A.O., Wu N., Suyalatu S., Zhao F., Zhao J., Tabikhanova L.E., Chen M., Bai H.This work is licensed under a Creative Commons Attribution 4.0 License.https://vavilov.elpub.ru/jour/article/view/1144

Ишемический инсульт, вызванный прерыванием потока крови в мозг, представляет серьезную медицинскую проблему, являясь фактором риска смерти и потери трудоспособности. Недавние исследования геномных ассоциаций (genome-wide association studies – GWAS) определили более 40 общих вариантов геномных последовательностей, связанных с ишемическим инсультом. Однако результаты не могут быть одинаково применимы для различных мировых популяций. В данной работе мы рассматриваем гипотезу о том, что население Северной Азии, живущее в суровых условиях окружающей среды, может иметь уникальный спектр генов риска развития заболеваний сердечно-сосудистой системы. Исследованы гены-кандидаты предрасположенности к ишемическому инсульту в популяции монголов Китая, которая не была изучена ранее. В исследование были включены в общей сложности 167 больных с инсультом и 176 здоровых лиц. Генотипирование выполнено с помощью секвенирования ампликонов. Обнаружена ассоциация развития ишемического инсульта с одиночными нуклеотидными полиморфизмами в генах NINJ2 (rs12425791) и ALDH2 (rs2238151), а также в межгенном участке rs9536591. Среди генов-кандидатов наибольший вклад в развитие инсульта вносит NINJ2 (rs12425791). Ген ALDH2 кодирует митохондриальный фермент альдегиддегидрогеназу, вовлеченную в оксидативный путь метаболизма алкоголя. Пол, возраст, индекс массы тела и высокое кровяное давление также могут быть факторами риска. Данная работа показала существующую гетерогенность между китайской и другими популяционными выборками. Наше исследование предлагает новый взгляд на взаимодействие генотипа и факторов окружающей среды при развитии ишемического инсульта в монгольском населении.

Ischemic stroke is caused by an interruption in the flow of blood to the brain and a risk factor for death and disability. Recent genome-wide association studies have identified more than 40 common sequence variants associated with ischemic stroke. However, the results are not always the same in populations with different genetic backgrounds. In the present study, we evaluated a hypothesis that a North Asian population living in a geographic area with unusually harsh environmental conditions would develop unique genetic risks. We investigated the candidate genes for ischemic stroke and risk factors in a Chinese Mongolian population which has not been explored previously. A total of 167 stroke cases and 176 controls were included in the study. Genotyping was performed by amplicon sequencing. The association was detected with single nucleotide polymorphisms (SNPs) located within genes NINJ2 (rs12425791) and ALDH2 (rs2238151) as well as intergenic rs9536591 were significantly associated with ischemic stroke, of which SNP rs12425791 of the NINJ2 gene was the strongest association. ALDH2 gene encodes mitochondrial aldehyde dehydrogenase, involved in the oxidative pathway of alcohol metabolism. Sex, age, body mass index and high blood pressure might be the risk factors. The current work also demonstrated genetic heterogeneity exists between Chinese and other populations. Our study provided the new insights into the genetic basis and environmental factors of ischemic stroke in Mongolian population.

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The authors declare that there are no conflicts of interest present.