vavilovВавиловский журнал генетики и селекцииVavilov Journal of Genetics and Breeding2500-3259Institute of Cytology and Genetics of Siberian Branch of the RAS10.18699/VJ17.277vavilov-1179Research ArticleГЕНЕТИЧЕСКИЕ РЕСУРСЫGENETIC RESOURCESХарактеристика некоторых аборигенных дагестанских сортов винограда методом SSR-анализа и по основным ампелографическим признакам листьевCharacterisation of native Dagestan grape cultivars using SSR-analysis and the main ampelographic features of the leavesИльницкаяЕ. Т.IlnitskayaE. T.ilnitskaya79@mail.ruСупрунИ. И.SuprunI. I.ilnitskaya79@mail.ruНаумоваЛ. Г.NaumovaL. G.ilnitskaya79@mail.ruТокмаковС. В.TokmakovS. V.ilnitskaya79@mail.ruГаничВ. А.GanichV. A.ilnitskaya79@mail.ruСеверо-Кавказский зональный научно-исследовательский институт садоводства и виноградарства, КраснодарРоссияNorth Caucasian Regional Research Institute of Horticulture and Viticulture, KrasnodarRussian FederationВсероссийский научно-исследовательский институт виноградарства и виноделия им. Я.И. Потапенко, НовочеркасскРоссияAll-Russian Research Institute of Viticulture and Winemaking named after Ya.I. Potapenko, NovocherkasskRussian Federation201728112017216617622Copyright © Ильницкая Е.Т., Супрун И.И., Наумова Л.Г., Токмаков С.В., Ганич В.А., 20172017Ильницкая Е.Т., Супрун И.И., Наумова Л.Г., Токмаков С.В., Ганич В.А.Ilnitskaya E.T., Suprun I.I., Naumova L.G., Tokmakov S.V., Ganich V.A.This work is licensed under a Creative Commons Attribution 4.0 License.https://vavilov.elpub.ru/jour/article/view/1179

Местные, стародавние сорта различных регионов возделывания винограда – важная часть мирового генофонда культуры. Дагестан – один из древнейших регионов виноградарства в Российской Федерации. Некоторые дагестанские аборигенные сорта винограда возделываются в промышленном масштабе, другие же обнаруживаются в единичных экземплярах. Изучению аборигенного генофонда уделяется особое внимание во всех странах, производящих виноград. В настоящее время наиболее информативным методом анализа генотипов растений являются исследования на уровне ДНК. Основные характеристики листьев сортов винограда – важнейший ампелографический признак. Сорта Агадаи, Алый терский, Бор кара, Будай шули, Гок ала, Гок изюм, Махбор цибил, Яй изюм белый, Яй изюм розовый изучены нами с применением данных подходов. В результате исследования получены ДНК-профили девяти аборигенных дагестанских сортов винограда по микросателлитным локусам VVMD5, VVMD7, VVMD27, VVS2, VrZAG62 и VrZAG79 с использованием автоматического генетического анализатора ABI Prism 3130. Указанные SSR-маркеры рекомендованы союзом генетиков винограда как основные для генотипирования Vitis vinifera L. Изученные генотипы обладают различными комбинациями аллелей проанализированных локусов. Оценка генетического сходства сортов по результатам микросателлитного анализа показала, что генотипы сортов Махбор цибил и Алый терский более близки к западно-европейскому генофонду V. vinifera L., чем другие аборигенные сорта в изученной выборке. Дагестанские сорта винограда описаны также по основным признакам сформировавшегося листа, согласно методике международной организации винограда и вина. По результатам анализа признаков листовой пластинки и по данным SSR-профилирования отмечена близость сортов Гок ала и Агадаи.

 

Local, ancient grape cultivars of different cultivation regions are important part of grapevine genetic resources. Dagestan is one of the oldest regions of viticulture in the Russian Federation. Some Dagestan aboriginal grape varieties are cultivated on an industrial scale, while others are found in single numbers. The study of the native gene pool is given special attention in all grapes producing countries of the world. Currently, the most informative method of plant genotypes analysis is the study at the DNA level. The main features of the leaves of grape varieties are a key ampelographical characteristic. We studied cultivars Agadai, Alyi terskyi, Bor kara, Buday shuli, Gok ala, Gok izyum, Mahbor cibil, Yai izyum beliy, Yai izyum rozovyi by using these approaches. DNA profiles of 9 local Dagestan grape cultivars were obtained on microsatellite loci VVMD5, VVMD7, VVMD27, VVS2, VrZAG62 and VrZAG79 using an automated genetic analyzer ABI Prism 3130. The SSR-markers are recommended as the main for Vitis vinifera L. genotyping. The cultivars analyzed have different sets of allele combination by the loci studied. Evaluation of the genetic similarity of cultivars according to the results of microsatellite analysis showed that the genotypes of Mahbоr cibil and Aliy terskiy are closer to the Western European gene pool of V. vinifera L. than any other native varieties in the sample studied. In addition, the grapevine cultivars studied were described for the main features of the formed leaves according to the method of the international organization of vine and wine. The similarity of cultivars Gok Ala and Agadai was shown by the results of analysis of the leaves characteristics and according to SSR-profiling.

 

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The authors declare that there are no conflicts of interest present.