vavilovВавиловский журнал генетики и селекцииVavilov Journal of Genetics and Breeding2500-3259Institute of Cytology and Genetics of Siberian Branch of the RAS10.18699/VJ17.284vavilov-1186Research ArticleГЕНОМИКА И ТРАНСКРИПТОМИКАPLANT GENETICS AND BREEDINGИспользование синтетической формы Авродес для передачи устойчивости к листовой ржавчине от Aegilops speltoides мягкой пшеницеUse of a synthetic form Avrodes for transfer of leaf rust resistance from Aegilops speltoides to common wheatДавоянР. О.DavoyanR. O.davoyanro@mail.ruБебякинаИ. В.BebyakinaI. V.davoyanro@mail.ruДавоянЭ. Р.DavoyanE. R.davoyanro@mail.ruМиковД. С.MikovD. S.davoyanro@mail.ruБадаеваЕ. Д.BadaevaE. D.davoyanro@mail.ruАдонинаИ. Г.AdoninaI. G.davoyanro@mail.ruСалинаЕ. А.SalinaE. A.davoyanro@mail.ruЗинченкоА. С.ZinchencoA. N.davoyanro@mail.ruЗубановаЮ. С.ZubanovaY. S.davoyanro@mail.ruКраснодарский научно-исследовательский институт сельского хозяйства им. П.П. Лукьяненко, КраснодарРоссияKrasnodar Lukyanenko Research Institute of Agriculture, KrasnodarRussian FederationИнститут общей генетики им. Н.И. Вавилова Российской академии наук, МоскваРоссияVavilov Institute of General Genetics RAS, MoscowRussian FederationФедеральный исследовательский центр Институт цитологии и генетики Сибирского отделения Российской академии наук, НовосибирскРоссияInstitute of Cytology and Genetics SB RAS, NovosibirskRussian Federation201728112017216663670Copyright © Давоян Р.О., Бебякина И.В., Давоян Э.Р., Миков Д.С., Бадаева Е.Д., Адонина И.Г., Салина Е.А., Зинченко А.С., Зубанова Ю.С., 20172017Давоян Р.О., Бебякина И.В., Давоян Э.Р., Миков Д.С., Бадаева Е.Д., Адонина И.Г., Салина Е.А., Зинченко А.С., Зубанова Ю.С.Davoyan R.O., Bebyakina I.V., Davoyan E.R., Mikov D.S., Badaeva E.D., Adonina I.G., Salina E.A., Zinchenco A.N., Zubanova Y.S.This work is licensed under a Creative Commons Attribution 4.0 License.https://vavilov.elpub.ru/jour/article/view/1186

Диплоидный сородич мягкой пшеницы Aegilops speltoides является ценным источником генов устойчивости к болезням. Для передачи от этого вида мягкой пшенице устойчивости к листовой ржавчине в качестве «мостика» была использована синтетическая форма Aвродес (BBAASS). Оценка 115 Triticum aestivum/Авродес интрогрессивных линий выявила их различие по устойчивости к листовой ржавчине – от среднеустойчивых до высокоустойчивых. Тестирование интрогрессивных линий с помощью молекулярных маркеров показало, что отдельные линии имеют гены устойчивости Lr28 и Lr35, наследуемые от синтетической формы Авродес. В то же время у большей части устойчивых линий их присутствие не было выявлено. Анализ мейотической конъюгации хромосом F1 гибридов показал, что генетический материал от синтетической формы Авродес передается в основном посредством транслокаций. С использованием методов C-окрашивания и FISH идентифицированы линии с транслокациями на хромосомах 2D и 5D. Ни один из переданных ранее генов устойчивости от Ae. speltoides не имеет локализацию на хромосомах 2D и 5D. Таким образом, можно предположить передачу новых генов устойчивости к листовой ржавчине от Ae. speltoides мягкой пшенице. Проведено изучение интрогрессивных линий по продуктивности и технологическим характеристикам зерна. Линии AA60n9 и D37n10 сочетают высокую устойчивость к листовой ржавчине с хорошими характеристиками по продуктивности и технологическими показателями зерна. Полученные результаты свидетельствуют о генетическом разнообразии и ценности исследуемых интрогрессивных линий для селекции мягкой пшеницы на устойчивость к листовой ржавчине.

 

Diploid wild relative of wheat – Aegilops speltoides – is a valuable source of genes for resistance to diseases. The synthetic form Avrodes (BBAASS) was used as a bridge to transfer leaf rust resistance genes from Ae. speltoides to common wheat. Introgression lines obtained from crosses of Avrodes and susceptible common wheat cultivars were evaluated in a field leaf rust nursery. Resistance levels varied from high to moderate. Testing of lines with the use of molecular markers has shown that some lines have the Lr28 and Lr35 genes inherited from synthetic form Avrodes. The majority of resistance lines have not been found to carry these genes. The Lr47 and Lr51 genes were not identified in the Avrodes and introgression lines. The analysis of chromosome pairing in F1 hybrids showed that the transfer of a genetic material from Avrodes to common wheat basically occurs through translocations. Lines with translocations on chromosomes 2D and 5D were identified by C-banding and FISH. The translocations differed in chromosomal location from known leaf resistance genes transferred to common wheat from Ae. speltoides. Hence it was assumed that new genes were introduced into the common wheat genome from Ae. speltoides. Introgression lines have been studied for productivity and technological qualities of grain. Lines AA60n9 and D37n10 combine high resistance to leaf rust with good characteristics of productivity and technological qualities of grain. The received results demonstrate a genetic diversity and a value of the investigated introgression lines for breeding of common wheat.

 

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The authors declare that there are no conflicts of interest present.