vavilovВавиловский журнал генетики и селекцииVavilov Journal of Genetics and Breeding2500-3259Institute of Cytology and Genetics of Siberian Branch of the RAS10.18699/VJ17.28-ovavilov-1187Research ArticleГЕНОМИКА И ТРАНСКРИПТОМИКАPLANT GENETICS AND BREEDINGВнутривидовая изменчивость Puccinia triticina на гексаплоидных видах Triticum и Aegilops trivialisIntraspecific variability of Puccinia triticina on hexaploid Triticum species and Aegilops trivialisГультяеваЕ. И.GultyaevaE. I.eigultyaeva@gmail.comШайдаюкЕ. Л.ShaydayukE. L.eigultyaeva@gmail.comКазарцевИ. А.KazartsevI. A.eigultyaeva@gmail.comВсероссийский научно-исследовательский институт защиты растений, Санкт-Петербург, ПушкинРоссияAll-Russian Institute of Plant Protection, St. Petersburg, PushkinRussian Federation201728112017216671676Copyright © Гультяева Е.И., Шайдаюк Е.Л., Казарцев И.А., 20172017Гультяева Е.И., Шайдаюк Е.Л., Казарцев И.А.Gultyaeva E.I., Shaydayuk E.L., Kazartsev I.A.This work is licensed under a Creative Commons Attribution 4.0 License.https://vavilov.elpub.ru/jour/article/view/1187

Анализ внутривидовой дифференциации патогена – необходимый этап при разработке стратегии селекции пшеницы на устойчивость к бурой ржавчине. Для характеристики популяций Puccinia triticina Erikss. могут быть использованы разные признаки, включая вирулентность к линиям серии Thatcher с генами устойчивости к бурой ржавчине (TcLr) и молекулярные маркеры. Цель работы – изучение внутривидовой изменчивости гриба P. triticina на гексаплоидных видах Triticum L. и Aegilops trivialis (Zhuk.) Migusch. et Chak. с помощью SSR-маркеров. Листья образцов гексаплоид- ных видов Triticum aestivum L., T. compactum Host., T. macha Dekapr. et Menabde, T. petropavlovskyi Udacz. et Migusch., T. spelta L., T. sphaerococcum Perc., T. vavilovii Jakubz. (геном BВAuAuDD), Ae. trivialis (геном Dc Dc DDМM) с урединиопустулами собирали на Дагестанской опытной станции ВИР (г. Дербент) в 2014 г. Для SSR-анализа использовали 109 монопустульных изолятов гриба, ранее охарактеризованных по вирулентности к 20 TcLr-линиям. Изучение полиморфизма 18 микросателлитных локусов позволило выявить 16 SSR-генотипов. Генетическое сходство между коллекциями изолятов с гексаплоидных видов пшеницы по молекулярным маркерам было существенно выше, чем по признаку вирулентности. Показано высокое генетическое сходство между дербентскими изолятами P. triticina, полученными с гексаплоидных видов родов Triticum (в том числе и мягкой пшеницы) и Ae. trivialis.

 

The analysis of pathogen intraspecific differentiation is a necessary stage for the development of a strategy of wheat leaf rust resistance breeding. To characterize the structure of the populations, virulence to Thatcher lines with leaf rust resistance genes (TcLr) and microsatellite markers may be used. The purpose of the present research is to study the intraspecific variability of the fungus Puccinia triticina Erikss. on Triticum L. hexaploid species and Aegilops trivialis (Zhuk.) Migusch. et Chak. using SSR-markers. Leaves of Triticum aestivum L., T. compactum Host., T. macha Dekapr. et Menabde, T. petropavlovskyi Udacz. et Migusch., T. spelta L., T. sphaerococcum Perc., T. vavilovii Jakubz. and Ae. trivialis with uredinia were collected on the experimental field of the Dagestan experiment station of VIR (Derbent) in 2014. For SSR-analysis 109, monopustule isolates previously characterized for virulence to 20 TcLr-lines were used. As a result of 18 microsatellite loci polymorphism analysis, 16 genotypes were identified. Genetic similarity between collections of isolates from the hexaploid types of wheat was significantly higher based on microsatellite markers than based on virulence. Microsatellite analysis confirmed a high similarity between Derbent isolates of P. triticina coming from the hexaploid Triticum species (including isolates from common wheat) and Aegilops trivialis.

 

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The authors declare that there are no conflicts of interest present.