vavilovВавиловский журнал генетики и селекцииVavilov Journal of Genetics and Breeding2500-3259Institute of Cytology and Genetics of Siberian Branch of the RAS10.18699/VJ17.286vavilov-1189Research ArticleГЕНОМИКА И ТРАНСКРИПТОМИКАPLANT GENETICS AND BREEDINGВлияние лигнификации и минерализации тканей листа на устойчивость к бурой ржавчине растений мягкой пшеницыInfluence of a lignification and mineralization of leaf tissues on resistance to a brown rust in common wheat plantsКоноваловА. А.KonovalovA. A.konov@bionet.nsc.ruШундринаИ. К.ShundrinaI. K.konov@bionet.nsc.ruКарповаЕ. В.KarpovaE. V.konov@bionet.nsc.ruЕльцовИ. В.EltsovI. V.konov@bionet.nsc.ruОрловаЕ. А.OrlovaE. A.konov@bionet.nsc.ruГончаровН. П.GoncharovN. P.konov@bionet.nsc.ruФедеральный исследовательский центр Институт цитологии и генетики Сибирского отделения Российской академии наук, НовосибирскРоссияInstitute of Cytology and Genetics SB RAS, NovosibirskRussian FederationНовосибирский институт органической химии им. Н.Н. Ворожцова Сибирского отделения Российской академии наук, Новосибирск; Новосибирский национальный исследовательский государственный университет, НовосибирскРоссияNovosibirsk Institute of Organic Chemistry SB RAS, Novosibirsk; Novosibirsk State University, NovosibirskRussian FederationНовосибирский национальный исследовательский государственный университет, НовосибирскРоссияNovosibirsk State University, NovosibirskRussian FederationСибирский научно-исследовательский институт растениеводства и селекции – филиал ИЦиГ СО РАН, Новосибирская область, Новосибирский район, пос. КраснообскРоссияSiberian Research Institute of Plant Industry and Breeding – Branch of the Institute of Cytology and Genetics SB RAS, Krasnoobsk, Novosibirsk regionRussian FederationФедеральный исследовательский центр Институт цитологии и генетики Сибирского отделения Российской академии наук, Новосибирск; Новосибирский государственный аграрный университет, НовосибирскРоссияInstitute of Cytology and Genetics SB RAS, Novosibirsk; Novosibirsk State Agrarian University, NovosibirskRussian Federation201729112017216686693Copyright © Коновалов А.А., Шундрина И.К., Карпова Е.В., Ельцов И.В., Орлова Е.А., Гончаров Н.П., 20172017Коновалов А.А., Шундрина И.К., Карпова Е.В., Ельцов И.В., Орлова Е.А., Гончаров Н.П.Konovalov A.A., Shundrina I.K., Karpova E.V., Eltsov I.V., Orlova E.A., Goncharov N.P.This work is licensed under a Creative Commons Attribution 4.0 License.https://vavilov.elpub.ru/jour/article/view/1189

Один из ключевых ферментов ароматического обмена растений, CAD (cinnamil alcoholdehydrogenase – дегидрогеназа коричного спирта; EC 1.1.1.195), у ряда видов растений оказывает влияние на содержание ароматических веществ и на защитные свойства тканей от грибных инфекций. При изучении коллекции сортообразцов мягкой пшеницы Triticum aestivum L., полиморфных по спектрам CAD, обнаружены различия по степени поражения листовой ржавчиной (возбудитель Puccinia recondita f. sp. tritici). Целью работы было изучение особенностей структуры и химического состава тканей листа, способствующих повышенной устойчивости. На фитопатологическом участке СибНИИРС на фоне искусственного заражения спорами бурой листовой ржавчины два образца яровой мягкой пшеницы 3-13-15-4 и 3-4-14-3 поражались соответственно на 1–5 и на 30 %. У этих контрастных по устойчивости образцов был проведен анализ содержания различных веществ в тканях листьев. На поверхности листа более устойчивого образца наблюдаются крупные бляшки, состоящие из минеральных веществ. В золе листьев и золе лигнина также обнаружены отличия по содержанию ряда минеральных элементов. Общее содержание лигнина на сухую массу листа различалось незначительно (14.2 и 12.3 % соответственно), однако есть различия по химическому составу лигнина. Можно предположить, что выявленные различия имеют отношение к разной степени поражения растений листовой ржавчиной. Следовательно, эти показатели можно использовать для диагностики потенциальной устойчивости сортообразцов пшеницы к грибной инфекции.

 

Aromatic substances in plant tissues can have protective effect against fungal diseases. One of the key enzymes in aromatic metabolism of plants, CAD (cinnamyl-alcohol dehydrogenase, dehydrogenase of cinnamyl alcohol; EC 1.1.1.195), at a number of species of plants exerts impact on the content of aromatic substances and on protective properties of tissues from fungal infections. When studying a collection of cultivars of bread wheat Triticum aestivum L., polymorphic on CAD, distinctions on extent of defeat are found by brown rust (with Puccinia recondita f. sp. Tritici as a causative agent). The purpose of the work was studying of features of structure and chemical composition of the tissues of a leaf promoting increased resistance. On a phytopathologic plot, against artificial infection with spores of a brown rust, two samples of spring bread wheat 3-13-15-4 and 3-4-14-3 were affected 1–5 % and 30 %, respectively. An analysis of various substances content in the leaf tissue at the contrast samples was conducted. Large plaques and spot consisting of mineral compounds were observed on the leaf surface of the more resistant plant. In ashes of leaves and ashes of a lignin differences in the maintenance of a number of mineral elements were also found. Lignin content on the dry mass of a leaf differed slightly (14.2 % vs 12.3 %), however there are differences in chemical composition. It is possible that the observed differences lead to afflict the plants with leaf rust to such different degrees. In that case these characteristics can be used for diagnostics of potential resistance of cultivars to fungal infection.

 

мягкая пшеницабурая ржавчиналигнификацияминерализацияспектроскопиядвумерный магнитный резонансcommon wheatbrown rustlignificationmineralizationspectroscopytwo-dimensional magnetic resonanceReferencesBagniewska-Zadworna A., Barakat A., Lakomy P., Smoliński D.J., Zadworny M. Lignin and lignans in plant defence: insight from expression profiling of cinnamyl alcohol dehydrogenase genes during development and following fungal infection in Populus. Plant Sci. 2014;229:111-121. DOI 10.1016/j.plantsci.2014.08.015.Bagniewska-Zadworna A., Barakat A., Lakomy P., Smoliński D.J., Zadworny M. Lignin and lignans in plant defence: insight from expression profiling of cinnamyl alcohol dehydrogenase genes during development and following fungal infection in Populus. Plant Sci. 2014;229:111-121. DOI 10.1016/j.plantsci.2014.08.015.Barber M.S., McConnell V.S., De Caux B.S. Antimicrobial intermediates of the general phenylpropanoid and lignin specific pathways. Phytochemistry. 2000;54(1):53-56. PMID: 10846747.Barber M.S., McConnell V.S., De Caux B.S. Antimicrobial intermediates of the general phenylpropanoid and lignin specific pathways. Phytochemistry. 2000;54(1):53-56. PMID: 10846747.Bélanger R.R., Benhamou N., Menzies J.G. Cytological evidence of an active role of silicon in wheat resistance to powdery mildew (Blumeria graminis f. sp. tritici). Phytopathology. 2003;93(4):402-412. DOI 10.1094/PHYTO.2003.93.4.402.Bélanger R.R., Benhamou N., Menzies J.G. Cytological evidence of an active role of silicon in wheat resistance to powdery mildew (Blumeria graminis f. sp. tritici). Phytopathology. 2003;93(4):402-412. DOI 10.1094/PHYTO.2003.93.4.402.Belhadj A., Saigne C., Telef N., Cluzet S., Bouscaut J., Corio-Cos-tet M.F., Mérillon J.M. Methyl jasmonate induces defense responses in grapevine and triggers protection against Erysiphe necator. J. Agric. Food Chem. 2006;54(24):9119-9125.Belhadj A., Saigne C., Telef N., Cluzet S., Bouscaut J., Corio-Cos-tet M.F., Mérillon J.M. Methyl jasmonate induces defense responses in grapevine and triggers protection against Erysiphe necator. J. Agric. Food Chem. 2006;54(24):9119-9125.Belyaev D.K. (Ed.) Genetika izofermentov [The Genetics of Isoenzymes]. Novosibirsk: Nauka Publ., 1977. (in Russian)Belyaev D.K. (Ed.) Genetika izofermentov [The Genetics of Isoenzymes]. Novosibirsk: Nauka Publ., 1977. (in Russian)Belyy V.A., Alekseev I.N., Sadykov R.A. Study of the chemical structure of lignins from golden root (Rhodiola rosea L.) and crowned saw-wort (Serratula coronata L.) by 2D nuclear magnetic resonance spectroscopy. Izvestiya Komi nauchnogo tsentra UrO RAN = Proceedings of the Komi Research Center. 2012;3(11):20-26. (in Russian)Belyy V.A., Alekseev I.N., Sadykov R.A. Study of the chemical structure of lignins from golden root (Rhodiola rosea L.) and crowned saw-wort (Serratula coronata L.) by 2D nuclear magnetic resonance spectroscopy. Izvestiya Komi nauchnogo tsentra UrO RAN = Proceedings of the Komi Research Center. 2012;3(11):20-26. (in Russian)Benhamou N., Bélanger R.R. Induction of systemic resistance to Pythi¬um damping-off in cucumber plants by benzothiadiazole: Ultrastructure and cytochemistry of the host response. Plant J. 1998;14(1):13-21. DOI 10.1046/j.1365-313X.1998.00088.x.Benhamou N., Bélanger R.R. Induction of systemic resistance to Pythi¬um damping-off in cucumber plants by benzothiadiazole: Ultrastructure and cytochemistry of the host response. Plant J. 1998;14(1):13-21. DOI 10.1046/j.1365-313X.1998.00088.x.Chesnokov Yu.V. Pathogen resistance in plants of plants (review of foreign literature). Selskokhozyaystvennaya biologiya = Agricultural Biology. 2007;1:16-35. (in Russian)Chesnokov Yu.V. Pathogen resistance in plants of plants (review of foreign literature). Selskokhozyaystvennaya biologiya = Agricultural Biology. 2007;1:16-35. (in Russian)del Río J.C., Rencoret J., Prinsen P., Martínez Á.T., Ralph J., Gutiérrez A. Structural characterization of wheat straw lignin as revealed by analytical pyrolysis, 2D-NMR, and reductive cleavage methods. J. Agric. Food Chem. 2012;60(23):5922-5935. DOI 10.1021/ jf301002n.del Río J.C., Rencoret J., Prinsen P., Martínez Á.T., Ralph J., Gutiérrez A. Structural characterization of wheat straw lignin as revealed by analytical pyrolysis, 2D-NMR, and reductive cleavage methods. J. Agric. Food Chem. 2012;60(23):5922-5935. DOI 10.1021/ jf301002n.Fauteux F., Rémus-Borel W., Menzies J.G., Bélanger R.R. Silicon and plant disease resistance against pathogenic fungi. FEMS Microbiol. Lett. 2005;249(1):1-6. DOI 10.1016/j.femsle.2005.06.034.Fauteux F., Rémus-Borel W., Menzies J.G., Bélanger R.R. Silicon and plant disease resistance against pathogenic fungi. FEMS Microbiol. Lett. 2005;249(1):1-6. DOI 10.1016/j.femsle.2005.06.034.Gaskell J., Marty A., Mozuch M., Kersten P.J., BonDurant S.S., Sa-bat G., Azarpira A., Ralph J., Skyba O., Mansfield S.D., Blanch-ette R.A., Cullen D. Influence of Populus genotype on gene expression by the wood decay fungus Phanerochaete chrysosporium. Appl. Environ. Microbiol. 2014;80(18):5828-5835. DOI 10.1128/ AEM.01604-14.Gaskell J., Marty A., Mozuch M., Kersten P.J., BonDurant S.S., Sa-bat G., Azarpira A., Ralph J., Skyba O., Mansfield S.D., Blanch-ette R.A., Cullen D. Influence of Populus genotype on gene expression by the wood decay fungus Phanerochaete chrysosporium. Appl. Environ. Microbiol. 2014;80(18):5828-5835. DOI 10.1128/ AEM.01604-14.Goncharov N.P., Konovalov A.A., Moiseeva E.A. Influence of short-term vernalization on the expression of the Aadh1¬Spn gene and the growth habit of plants in hybrids of rye Secale cereale L. to S. montanum Guss. Sibirskiy vestnik selskokhozyaystvennoy nauki = Siberian Herald of Agricultural Sciences. 2012;5:19-25. (in Russian)Goncharov N.P., Konovalov A.A., Moiseeva E.A. Influence of short-term vernalization on the expression of the Aadh1¬Spn gene and the growth habit of plants in hybrids of rye Secale cereale L. to S. montanum Guss. Sibirskiy vestnik selskokhozyaystvennoy nauki = Siberian Herald of Agricultural Sciences. 2012;5:19-25. (in Russian)Hano C., Addi M., Bensaddek L., Cronier D., Baltora-Rosset S., Dous-sot J., Maury S. Mesnard F., Chabbert B., Hawkins S., Laine E., Lamblin F. Differential accumulation of monolignol-derived compounds in elicited flax (Linum usitatissimum) cell suspension cultures. Planta. 2006;223:975-989.Hano C., Addi M., Bensaddek L., Cronier D., Baltora-Rosset S., Dous-sot J., Maury S. Mesnard F., Chabbert B., Hawkins S., Laine E., Lamblin F. Differential accumulation of monolignol-derived compounds in elicited flax (Linum usitatissimum) cell suspension cultures. Planta. 2006;223:975-989.Hart G.E., Gale M.D., McIntosh R.A. Linkage maps of Triticum aestivum (Hexaploid wheat, 2n = 42, Genomes A, B & D) and T. tauschii (2n = 14, Genome D). Genetic Maps. 6 ed. S.J. O’Brien (Ed.). N. Y.: Cold Spring Harbor Lab. Press, 1993;6.204-6.219.Hart G.E., Gale M.D., McIntosh R.A. Linkage maps of Triticum aestivum (Hexaploid wheat, 2n = 42, Genomes A, B & D) and T. tauschii (2n = 14, Genome D). Genetic Maps. 6 ed. S.J. O’Brien (Ed.). N. Y.: Cold Spring Harbor Lab. Press, 1993;6.204-6.219.Hijwegen T. Lignification, a possible mechanism of active resistance against pathogens. Neth. J. Plant Pathol. 1963;69:314-317.Hijwegen T. Lignification, a possible mechanism of active resistance against pathogens. Neth. J. Plant Pathol. 1963;69:314-317.Konovalov A.A., Shundrina I.K., Karpova E.V. Polymorphism of lignification enzymes in plants: Functional importance and applied aspects. Biology Bulletin Reviews. 2016;6(2):149-163.Konovalov A.A., Shundrina I.K., Karpova E.V. Polymorphism of lignification enzymes in plants: Functional importance and applied aspects. Biology Bulletin Reviews. 2016;6(2):149-163.Konovalov A.A., Shundrina I.K., Karpova E.V., Goncharov N.P.,Konovalov A.A., Shundrina I.K., Karpova E.V., Goncharov N.P.,Kondratenko E.Ya. Chromosomal localization of aromatic alcohol dehydrogenase fast-migrating isoenzyme AAdh1F (CAD1F) gene in Triticum aestivum L. bread wheat. Russian Journal of Genetics. 2016;52(10):1110-1116.Kondratenko E.Ya. Chromosomal localization of aromatic alcohol dehydrogenase fast-migrating isoenzyme AAdh1F (CAD1F) gene in Triticum aestivum L. bread wheat. Russian Journal of Genetics. 2016;52(10):1110-1116.Konovalov A.A., Shundrina I.K., Karpova E.V., Nefedov A.A., Goncharov N.P. Inheritance and phenotype expression of functional and null alleles of aromatic alcohol dehydrogenase (CAD) in diploid wheats. Russian Journal of Genetics. 2014;50(11):1161-1168.Konovalov A.A., Shundrina I.K., Karpova E.V., Nefedov A.A., Goncharov N.P. Inheritance and phenotype expression of functional and null alleles of aromatic alcohol dehydrogenase (CAD) in diploid wheats. Russian Journal of Genetics. 2014;50(11):1161-1168.Konovalov A.A., Shundrina I.K., Karpova E.V., Orlova E.A., Goncharov N.P. Influence of a lignification and mineralization of leaf tissues on resistance of bread wheat plants to a brown rust. Plant Genetics and Genomics for Food Security. The 1st Int. Workshop: Abstract Book. Novosibirsk, Russia, Aug. 26–28. Novosibirsk, 2016;27.Konovalov A.A., Shundrina I.K., Karpova E.V., Orlova E.A., Goncharov N.P. Influence of a lignification and mineralization of leaf tissues on resistance of bread wheat plants to a brown rust. Plant Genetics and Genomics for Food Security. The 1st Int. Workshop: Abstract Book. Novosibirsk, Russia, Aug. 26–28. Novosibirsk, 2016;27.Kumar S., Röder M.S., Kumar S., Singh R.P., Joshi A.K., Kumar U. Mapping of spot blotch disease resistance using NDVI as substitute to visual observation in wheat (Triticum aestivum L.). Plant Genetics and Genomics for Food Security. The 1st Int. Workshop: Abstract Book. Novosibirsk, Russia, Aug. 26–28. Novosibirsk, 2016;32.Kumar S., Röder M.S., Kumar S., Singh R.P., Joshi A.K., Kumar U. Mapping of spot blotch disease resistance using NDVI as substitute to visual observation in wheat (Triticum aestivum L.). Plant Genetics and Genomics for Food Security. The 1st Int. Workshop: Abstract Book. Novosibirsk, Russia, Aug. 26–28. Novosibirsk, 2016;32.Leonova I.N., Roder M.S., Kalinina N.P., Budashkina E.B. The genetic analysis and localization of the loci controlling the resistance of Tri¬ ticum aestivum × T. timopheevii introgression lines to leaf rust. Genetika = Genetics (Moscow). 2008;44(12):1652-1659. (in Russian)Leonova I.N., Roder M.S., Kalinina N.P., Budashkina E.B. The genetic analysis and localization of the loci controlling the resistance of Tri¬ ticum aestivum × T. timopheevii introgression lines to leaf rust. Genetika = Genetics (Moscow). 2008;44(12):1652-1659. (in Russian)Li X., Ma D., Chen J., Pu G., Ji Y., Lei C., Du Z., Liu B., Ye H., Wang H. Biochemical characterization and identification of a cinnamyl alcohol dehydrogenase from Artemisia annua. Plant Sci. 2012;193-194:85-95. DOI 10.1016/j.plantsci.2012.05.011.Li X., Ma D., Chen J., Pu G., Ji Y., Lei C., Du Z., Liu B., Ye H., Wang H. Biochemical characterization and identification of a cinnamyl alcohol dehydrogenase from Artemisia annua. Plant Sci. 2012;193-194:85-95. DOI 10.1016/j.plantsci.2012.05.011.McIntosh R.A., Devos K.M., Dubcovsky J., Rogers W.J., Morris C.F., Appels R., Anderson O.D. Catalogue of gene symbols for wheat: Supplement. Annual Wheat Newsletter. 2005;51:251-285.McIntosh R.A., Devos K.M., Dubcovsky J., Rogers W.J., Morris C.F., Appels R., Anderson O.D. Catalogue of gene symbols for wheat: Supplement. Annual Wheat Newsletter. 2005;51:251-285.Menden B., Kohlhoff M., Moerschbacher B.M. Wheat cells accumulate a syringyl-rich lignin during the hypersensitive resistance response. Phytochemistry. 2007;68(4):513-520.Menden B., Kohlhoff M., Moerschbacher B.M. Wheat cells accumulate a syringyl-rich lignin during the hypersensitive resistance response. Phytochemistry. 2007;68(4):513-520.Mitchell H.J., Hall J.L., Barber M.S. Elicitor-induced cinnamyl alcohol dehydrogenase activity in lignifying wheat (Triticum aestivum L.) leaves. Plant Physiol. 1994;104(2):551-556.Mitchell H.J., Hall J.L., Barber M.S. Elicitor-induced cinnamyl alcohol dehydrogenase activity in lignifying wheat (Triticum aestivum L.) leaves. Plant Physiol. 1994;104(2):551-556.Moerschbacher B.M., Noll U., Gorrichon L., Reisener H.J. Specific inhibition of lignification breaks hypersensitive resistance of wheat to stem rust. Plant Physiol. 1990;93(2):465-470. PMID: 16667489.Moerschbacher B.M., Noll U., Gorrichon L., Reisener H.J. Specific inhibition of lignification breaks hypersensitive resistance of wheat to stem rust. Plant Physiol. 1990;93(2):465-470. PMID: 16667489.Pillonel C., Hunziker P., Binder A. Multiple forms of the constitutive wheat cinnamyl alcohol dehydrogenase. J. Exp. Bot. 1992;43(248): 299-305.Pillonel C., Hunziker P., Binder A. Multiple forms of the constitutive wheat cinnamyl alcohol dehydrogenase. J. Exp. Bot. 1992;43(248): 299-305.Radchenko E.E. (Ed.) Izuchenie geneticheskikh resursov zernovykh kultur po ustoychivosti k vrednym organizmam: metodicheskoe posobie [Study of Genetic Resources for Pest Resistance in Grain Crops: Methodical Handbook]. Moscow: Rosselkhozakademiya Publ., 2008. (in Russian).Radchenko E.E. (Ed.) Izuchenie geneticheskikh resursov zernovykh kultur po ustoychivosti k vrednym organizmam: metodicheskoe posobie [Study of Genetic Resources for Pest Resistance in Grain Crops: Methodical Handbook]. Moscow: Rosselkhozakademiya Publ., 2008. (in Russian).Simons L., Bultman T.L., Sullivan T.J. Effects of methyl jasmonate and an endophytic fungus on plant resistance to insect herbivores. J. Chem. Ecology. 2008;34:1511-1517.Simons L., Bultman T.L., Sullivan T.J. Effects of methyl jasmonate and an endophytic fungus on plant resistance to insect herbivores. J. Chem. Ecology. 2008;34:1511-1517.Somssich I.E., Wernert P., Kiedrowski S., Hahlbrock K. Arabidopsis thaliana defense-related protein ELI3 is an aromatic alcohol: NADP+ oxidoreductase. Proc. Natl. Acad. Sci. USA. 1996;93:14199-14203.Somssich I.E., Wernert P., Kiedrowski S., Hahlbrock K. Arabidopsis thaliana defense-related protein ELI3 is an aromatic alcohol: NADP+ oxidoreductase. Proc. Natl. Acad. Sci. USA. 1996;93:14199-14203.Tronchet M., Balagué C., Kroj T., Jouanin L., Roby D. Cinnamyl alcohol dehydrogenases-C and D, key enzymes in lignin biosynthe-sis, play an essential role in disease resistance in Arabidopsis. Molecular Plant Pathology. 2010;11:83-92. DOI 10.1111/j.1364-3703. 2009.00578.x.Tronchet M., Balagué C., Kroj T., Jouanin L., Roby D. Cinnamyl alcohol dehydrogenases-C and D, key enzymes in lignin biosynthe-sis, play an essential role in disease resistance in Arabidopsis. Molecular Plant Pathology. 2010;11:83-92. DOI 10.1111/j.1364-3703. 2009.00578.x.Varbanova M., Porter K., Lu F., Ralph J., Hammerschmidt R., Jones A.D., Day B. Molecular and biochemical basis for stress-induced accumulation of free and bound p-coumaraldehyde in cucumber. Plant Physiol. 2011;157(3):1056-1066. DOI 10.1104/pp.111.184358.Varbanova M., Porter K., Lu F., Ralph J., Hammerschmidt R., Jones A.D., Day B. Molecular and biochemical basis for stress-induced accumulation of free and bound p-coumaraldehyde in cucumber. Plant Physiol. 2011;157(3):1056-1066. DOI 10.1104/pp.111.184358.Zaprometov M.N. Fenolnye soedineniya: rasprostranenie, metabolizm i funktsii v rasteniyakh [Phenolic Compounds: Distribution, Metabolism, and Functions in Plants]. Moscow: Nauka Publ., 1993. (in Russian)Zaprometov M.N. Fenolnye soedineniya: rasprostranenie, metabolizm i funktsii v rasteniyakh [Phenolic Compounds: Distribution, Metabolism, and Functions in Plants]. Moscow: Nauka Publ., 1993. (in Russian)

The authors declare that there are no conflicts of interest present.