vavilovВавиловский журнал генетики и селекцииVavilov Journal of Genetics and Breeding2500-3259Institute of Cytology and Genetics of Siberian Branch of the RAS10.18699/VJ17.26-ovavilov-1192Research ArticleМОЛЕКУЛЯРНАЯ ГЕНЕТИКА И КЛЕТОЧНАЯ БИОЛОГИЯMOLECULAR GENETICS AND CELL BIOLOGYРоль гена pAbp, кодирующего цитоплазматический поли(А)-связывающий белок, в сперматогенезе Drosophila melanogasterThe role of the pAbp gene encoding the cytoplasmic poly(A)-binding protein in spermatogenesis of Drosophila melanogasterБолоболоваЕ. У.BolobolovaE. U.elbol@bionet.nsc.ruБаричеваЭ. М.BarichevaE. M.elbol@bionet.nsc.ruДороговаН. В.DorogovaN. V.elbol@bionet.nsc.ruФедеральный исследовательский центр Институт цитологии и генетики Сибирского отделения Российской академии наук, НовосибирскРоссияInstitute of Cytology and Genetics SB RAS, NovosibirskRussian Federation201729112017216710716Copyright © Болоболова Е.У., Баричева Э.М., Дорогова Н.В., 20172017Болоболова Е.У., Баричева Э.М., Дорогова Н.В.Bolobolova E.U., Baricheva E.M., Dorogova N.V.This work is licensed under a Creative Commons Attribution 4.0 License.https://vavilov.elpub.ru/jour/article/view/1192

Поли(А)-связывающий белок (PABP) Drosophila melanogaster относится к цитоплазматическим PABP, участвующим в контроле инициации и терминации трансляции мРНК, сохранении ее стабильности, цитоплазматическом полиаденилировании/деаденилировании, деградации мРНК. Несмотря на всестороннюю структурную и биохимическую характеристику цитоплазматических PABP, относительно мало известно об их участии в процессах развития и дифференцировки. Вследствие того что большинство генов, кодирующих необходимые для сперматогенеза Drosophila белки, транскрибируется в первичных сперматоцитах, предполагают, что развитие сперматид определяется отложенной трансляцией, в регулировании которой участвует белок PABP. Утрата PABP приводит к гибели эмбрионов. Сочетание некоторых мутантных аллелей гена pAbp D. melanogaster является тканеспецифичным и приводит к нарушению сперматогенеза и стерильности самцов. Ранее были выявлены и описаны дефекты мейоза и цитокинеза, нарушения структуры небенкерна в сперматогенезе гипоморфных мутантов pAbp. Опубликованные данные дают возможность судить о действии мутантных аллелей pAbp на отдельные события сперматогенеза, но не охватывают весь процесс. Целью настоящей работы было детальное цитологическое исследование влияния гена pAbp на сперматогенез D. melanogaster с помощью флуоресцентной световой и электронной микроскопии. Показано, что начальное действие мутации гена pAbp на структуру клеток проявилось на стадии первичных сперматоцитов. Первичной структурной мишенью мутации был митохондриальный аппарат клеток. Изменение морфологии митохондрий мутанта pAbp включало набухание, уменьшение плотности матрикса и редукцию количества крист. Далее действие мутации проявилось плейотропно, вызвало нарушение формирования и деления небенкерна, образования аксиальных комплексов и привело к остановке сперматогенеза на стадии элонгации цист. На основании результатов проведенных исследований можно сделать вывод, что белок PABP D. melano­gas ter участвует во многих событиях сперматогенеза и играет критически важную роль в структурной дифференцировке гамет в спермиогенезе.

 

 

 

 

The Drosophila melanogaster pAbp gene encodes the cytoplasmic poly(A)-binding protein (PABP). Cytoplasmic PABPs participate in the metabolic pathways of the mRNA: translation initiation and termination, cytoplasmic polyadenylation/deadenylation, mRNA stability, mRNA degradation. Despite the extensive biochemical and structural characterization, relatively little is known about the biological roles of PABPs in the processes of cellular development and differentiation. In Drosophila spermatogenesis, posttranscriptional mechanisms of gene regulation play an important role, so cytoplasmic PABP can have significant function in this process. Deletion of PABP leads to embryonic lethality. However, some flies carrying combinations of mutant pAbp alleles survive but display male sterility and show defects in spermatogenesis. It has previously been shown that hypomorphic pAbp mutations cause a number of meiotic defects, abnormalities of Nebenkern formation. These data provide an insight into the effect of pAbp mutation on the individual events of spermatogenesis, but they do not cover the entire process. We studied spermatogenesis in pAbpallele heterozygotes by transmission electron and fluorescent light microscopy. We showed that cellular mitochondria were the primary structural target of the mutation. Abnormal mitochondria were less structured, swollen, had transparent matrix and depleted cristae. Further mutation had a polymorphous effect and induced anomalies in the ultrastructure of mature spermatocytes, defects in Nebenkern formation and division, axial complex formation, shutdown of spermatogenesis during spermatid elongation. Thus our data show a significant role of PABP in structural transformations of male germ cells during entire spermato-genesis.

 

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The authors declare that there are no conflicts of interest present.