vavilovВавиловский журнал генетики и селекцииVavilov Journal of Genetics and Breeding2500-3259Institute of Cytology and Genetics of Siberian Branch of the RAS10.18699/VJ17.296vavilov-1228Research ArticleФИЛОГЕНЕТИКАHIGH-THROUGHPUT PHENOTYPINGЭкспрессия генов системы катехоламинов в среднем мозге и реакция престимульного торможения у крыс с генетической кататониейExpression of catecholaminergic genes in the midbrain and prepulse inhibition in rats with a genetic catatoniaРязановаМ. А.RyazanovaM. A.

Новосибирск.

Novosibirsk.

ocean-2006@yandex.ruПрокудинаО. И.ProkudinaO. I.

Новосибирск.

Novosibirsk.

ПлеканчукВ. С. PlekanchukV. S.

Екатеринбург.

Yekaterinburg.

АлехинаТ. А.AlekhinaT. A.

Новосибирск.

Novosibirsk.

Федеральный исследовательский центр Институт цитологии и генетики Сибирского отделения Российской академии наук.РоссияInstitute of Cytology and Genetics SB RAS.Russian FederationУральский федеральный университет имени первого Президента России Б.Н. Ельцина.РоссияThe Ural Federal University named after the first President of Russia B.N. Yeltsin.Russian Federation201723122017217798803Copyright © Рязанова М.А., Прокудина О.И., Плеканчук В.С., Алехина Т.А., 20172017Рязанова М.А., Прокудина О.И., Плеканчук В.С., Алехина Т.А.Ryazanova M.A., Prokudina O.I.,  Plekanchuk V.S., Alekhina T.A.This work is licensed under a Creative Commons Attribution 4.0 License.https://vavilov.elpub.ru/jour/article/view/1228

В Институте цитологии и генетики СО РАН путем селекции на усиление реакции пассивно-оборонительного застывания в ответ на стрессорный стимул создана линия крыс ГК (от слов «генетическая» и «кататония»). Ранее было показано, что крысы линии ГК имеют ряд биохимических и поведенческих особенностей, гомологичных соответствующим характеристикам у больных шизофренией и депрессией. Реакцию престимульного торможения (PPI) рассматривают в качестве показателя, снижение которого может свидетельствовать о наличии психопатологии, в частности шизофрении. Исследования показывают, что норадренергическая система мозга влияет на проявление PPI через активацию центральных альфа-адренорецепторов. Известна также связь PPI с экспрессией катехол-О-метилтрансферазы. Целью работы было исследование реакции престимульного торможения у крыс инбредной линии ГК как гипотетической модели шизофренной патологии и изучение связи реакции престимульного торможения у крыс ГК с экспрессией мРНК генов тирозингидроксилазы, катехол-О-метилтрансферазы, альфа1А- и альфа2А-адренорецепторов в среднем мозге. Впервые выявлено снижение реакции престимульного торможения у крыс линии ГК по сравнению с крысами линии WAG при силе престимула как 75, так и 85 дБ, что может указывать на нарушение процесса фильтрации сенсомоторной информации в ЦНС у крыс линии ГК. Методом ПЦР в реальном времени показано снижение уровня экспрессии мРНК гена Adra1A у интактных крыс с ГК по сравнению с контрольными крысами линии WAG. Корреляционных взаимосвязей между экспрессией мРНК генов Adra1A, Adra2A, Th, Comt в среднем мозге и реакцией престимульного торможения у крыс линии ГК не обнаружено. Сниженная реакция престимульного торможения у крыс линии ГК свидетельствует в пользу функционального сходства линии как предполагаемой генетической модели шизофренной психопатологии с прототипом.

The GC rat strain (from the words “genetic” and “catatonia”) was created by selection for predisposition to passive-defensive reaction of catatonic freezing in response to stressing stimuli. Rats of the GC strain have previously demonstrated a number of biochemical and behavioral properties similar to those of patients with schizophrenia and depression. Prepulse inhibition (PPI) is widely explored as an important indicator, a decrease of which may be indicative of psychopathology, including schizophrenia. It has been established that the brain noradrenergic system influences the manifestation of PPI, in particular through the activation of central alpha-adrenoreceptors. Also known is the association between PPI and expression of catechol-O-methyltransferase. This study focuses on the reaction of prepulse inhibition in rats of the inbred GC strain, being considered as a hypothetical model of schizophrenia, as well as on the relation of prepulse inhibition to mRNA expression of tyrosine hydroxylase, catechol-O-methyltransferase, alpha1A- and alpha2Aadrenergic receptors in the midbrain of GC rats. For the first time, a decrease of PPI in GC rats compared with WAG rats was shown, both with a prepulse power of 75 dB and at 85 dB, which may indicate a violation of filtration of sensorimotor information into the central nervous system in GC rats. Real-time PCR showed a decrease in mRNA level of Adra1A in intact rats with genetic catatonia when compared to control WAG rats. There was observed no correlation between the expression of mRNA of the Adra1A, Adra2A, Th, and Comt genes in the midbrain and the PPI reaction in GC rats. The reduction of prepulse inhibition in GC rats indicates functional similarity of this genetic model of schizophrenic psychopathology with a prototype.

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The authors declare that there are no conflicts of interest present.