vavilovВавиловский журнал генетики и селекцииVavilov Journal of Genetics and Breeding2500-3259Institute of Cytology and Genetics of Siberian Branch of the RAS10.18699/VJ17.316vavilov-1271Research ArticleСИСТЕМНАЯ БИОЛОГИЯ И БИОМЕДИЦИНАSYSTEMS BIOLOGY AND BIOMEDICINEОтсроченные эффекты хирургического воздействия, перенесенного матерью в период ранней беременности, на артериальное давление и поведение потомков у крыс линии OXYSLong-term effects of maternal exposure to surgical stress at the earliest stage of pregnancy on blood pressure and behavior in offspring of OXYS ratsРожковаИ. Н.RozhkovaI. N.

Новосибирск.

Novosibirsk.

ИгонинаТ. Н.IgoninaT. N.

Новосибирск.

Novosibirsk.

РагаеваД. С.RagaevaD. S.

Новосибирск.

Novosibirsk.

ПетроваО. М. PetrovaO. M.

Новосибирск.

Novosibirsk.

БрусенцевЕ. Ю.BrusentsevE. Yu.

Новосибирск.

Novosibirsk.

НапримеровВ. А.NaprimerovV. A.

Новосибирск.

Novosibirsk.

АмстиславскийС. Я.AmstislavskyS. Ya.

Новосибирск.

Novosibirsk.

amstis@bionet.nsc.ruФедеральный исследовательский центр Институт цитологии и генетики Сибирского отделения Российской академии наук.РоссияInstitute of Cytology and Genetics SB RAS.Russian FederationФедеральный исследовательский центр Институт цитологии и генетики Сибирского отделения Российской академии наук; Новосибирский национальный исследовательский государственный университет.РоссияInstitute of Cytology and Genetics SB RAS; Novosibirsk State University.Russian FederationФедеральный исследовательский центр Институт цитологии и генетики Сибирского отделения Российской академии наук; Новосибирский государственный аграрный университет.РоссияInstitute of Cytology and Genetics SB RAS; Novosibirsk State Agrarian University.Russian Federation201720012018218937942Copyright © Рожкова И.Н., Игонина Т.Н., Рагаева Д.С., Петрова О.М., Брусенцев Е.Ю., Напримеров В.А., Амстиславский С.Я., 20182018Рожкова И.Н., Игонина Т.Н., Рагаева Д.С., Петрова О.М., Брусенцев Е.Ю., Напримеров В.А., Амстиславский С.Я.Rozhkova I.N., Igonina T.N., Ragaeva D.S.,  Petrova O.M., Brusentsev E.Y., Naprimerov V.A., Amstislavsky S.Y.This work is licensed under a Creative Commons Attribution 4.0 License.https://vavilov.elpub.ru/jour/article/view/1271

Применение некоторых вспомогательных репродуктивных технологий, в частности трансплантации эмбрионов, может вызывать различные физиологические и поведенческие изменения у потомства. Целью нашего исследования являлась проверка влияния хирургического вмешательства, используемого при трансплантации эмбрионов, на вес, артериальное давление и поведение в тестах «открытое поле» (ТОП) и «приподнятый крестообразный лабиринт» (ПКЛ) у взрослых потомков. В работе исследованы отсроченные эффекты хирургического воздействия на четвертые сутки беременности на потомков крыс линии OXYS. Самок линии OXYS спари­ вали с фертильными самцами той же линии, через 96 ч после обнаружения сперматозоидов во влагалищных мазках им проводили хирургическую операцию, имитирующую трансплантацию эмбрионов. У потомков самок, подвергавшихся хирургическому воздействию во время беременности (группа OXYS­PS), в возрасте 3 мес. измеряли вес тела, систолическое (САД) и диастолическое (ДАД) артериальное давление, а также исследовали поведение в тестах ТОП и ПКЛ. Контролем служили потомки интактных крыс OXYS. Средний вес у крыс линии OXYS не отличался от такового у крыс OXYS­PS. Крысы линий OXYS и OXYS­PS имели высокое САД, превышающее гипертензивный порог (150 мм рт. ст.), и высокое ДАД. При этом данные показатели достоверно выше у крыс OXYS­PS, чем у животных контрольной линии. У крыс OXYS­PS в тесте ТОП было снижено время, проведенное в центре арены, меньше исследованная область, а также снижено число стоек и их продолжительность по сравнению с крысами OXYS. Как показано в тесте ПКЛ, число выглядываний из закрытых рукавов и их длительность меньше у крыс OXYS­PS, чем в контроле. Таким образом, хирургическое воздействие, испытанное самкой OXYS на ранних стадиях беременности, приводит к следующим эффектам у потомков: повышение САД и ДАД, снижение исследовательской активности и повышение тревожности.

The use of some assisted reproductive technologies, in particular, embryo transfer, may cause various physiological and behavioral changes in the offspring. The purpose of our study was to study the effects of surgery (which is used for embryo transfer) done with pregnant dams on the weight, blood pressure and behavior in the open field and elevated plus­maze tests in adult offspring. Thus, long­term effects on the offspring after maternal exposure to surgical stress given to dams at the 4th day of pregnancy were studied in OXYS rats. OXYS females were mated in estrus with fertile males of the same strain. 96 hours after spermatozoa were found in vaginal smears the surgery (sham operation, imitating embryo transfer) was performed. Body weight (BW), systolic (SAP) and diastolic (DAP) arterial pressure as well as behavior in open field (OF) and elevated plus maze (EPM) tests were studied in the offspring of females exposed to surgical treatment during pregnancy (OXYS­PS) at the age of 3 mo. Untreated offspring of OXYS rats were used as controls. BW in naturally born OXYS rats did not differ from those of the OXYS­PS group. OXYS and OXYS­PS rats exhibited higher SAP (more than 150 mm Hg) and DAP; it is noteworthy that both SAP and DAP were higher in the OXYS­PS group than in the control group. The time spent in the center of arena, the area studied, the time and number of rearing were decreased in OXYS­PS rats in the OF test as compared to the OXYS controls. Moreover, OXYS­PS rats were characterized by the absence of grooming in the OF test. As was demonstrated by the EPM test, the duration and numbers of peeking out from closed arms were decreased in the OXYS­PS rats as compared to the OXYS controls. Thus, OXYS dams’ exposure to surgical stress at their early pregnancy led to such effects in the offspring as elevated SAP and DAP, decreased overall activity and increased anxiety.

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The authors declare that there are no conflicts of interest present.