vavilovВавиловский журнал генетики и селекцииVavilov Journal of Genetics and Breeding2500-3259Institute of Cytology and Genetics of Siberian Branch of the RAS10.18699/VJ18.326vavilov-1358Research ArticleГЕНЕТИКА И СЕЛЕКЦИЯ КАРТОФЕЛЯPOTATO GENETICS AND BREEDINGИССЛЕДОВАНИЕ КОЛЛЕКЦИОННЫХ ОБРАЗЦОВ КАРТОФЕЛЯ НА НАЛИЧИЕ ГЕНЕТИЧЕСКИХ МАРКЕРОВ УСТОЙЧИВОСТИ К ФИТОПАТОГЕНАМTESTING POTATO COLLECTION SAMPLES FOR THE PRESENCE OF GENES FOR RESISTANCE TO PHYTOPATHOGENS BY MEANS OF DNA MARKERSСайнаковаА. Б.SaynakovaA. B.

Томск

Tomsk

annasaynakova@vtomske.ruРомановаМ. С.RomanovaM. S.

Томск

Tomsk

КрасниковС. Н.KrasnikovS. N.

Томск

Tomsk

ЛитвинчукО. В.LitvinchukO. V.

Томск

Tomsk

АлексеевЯ. И.AlekseevYa. I.

Москва

Moscow

НикулинА. В.NikulinA. V.

Москва

Moscow

ТерентьеваЕ. В.TerentjevaE. V.

Москва

Moscow

Сибирский научно-исследовательский институт сельского хозяйства и торфа – филиал Сибирского федерального научного центра агробиотехнологий Российской академии наукРоссияSiberian Institute of Agriculture and Peat Research – the Branch of the Siberian Federal Agricultural Biotechnology Science Center RASRussian FederationВсероссийский научно-исследовательский институт сельскохозяйственной биотехнологии; ООО СинтолРоссияAll-Russia Research Institute of Agricultural Biotechnology; Limited Liability Company SyntolRussian FederationВсероссийский научно-исследовательский институт сельскохозяйственной биотехнологииРоссияAll-Russia Research Institute of Agricultural BiotechnologyRussian Federation2018210320182211824Copyright © Сайнакова А.Б., Романова М.С., Красников С.Н., Литвинчук О.В., Алексеев Я.И., Никулин А.В., Терентьева Е.В., 20182018Сайнакова А.Б., Романова М.С., Красников С.Н., Литвинчук О.В., Алексеев Я.И., Никулин А.В., Терентьева Е.В.Saynakova A.B., Romanova M.S., Krasnikov S.N., Litvinchuk O.V., Alekseev Y.I., Nikulin A.V., Terentjeva E.V.This work is licensed under a Creative Commons Attribution 4.0 License.https://vavilov.elpub.ru/jour/article/view/1358

Создание устойчивых к фитопатогенам сортов – наиболее надежный и экономичный способ снижения потерь урожайности картофеля. Выведение таких сортов возможно при использовании в селекционной работе генетических источников устойчивости. Применение ДНК-маркеров для идентификации ценных генотипов, в том числе форм с несколькими генами устойчивости, позволяет существенно повысить эффективность селекции. Разработка технологии мультиплексной ПЦР для одновременного тестирования сортов и селекционных линий по нескольким генам, контролирующим устойчивость к вирусам и нематодам, является новым подходом в использовании ДНК-маркеров. Цель работы: скрининг методом мультиплексной ПЦР образцов картофеля коллекции Нарымского отдела селекции и семеноводства СибНИИСХиТ – филиала СФНЦА РАН на наличие генов устойчивости к золотистой и бледной нематодам, раку, а также вирусам X и Y. Проведена оценка 40 образцов на присутствие в геноме генов устойчивости к раку картофеля (Sen1), к вирусу X (Rx),  вирусу Y (Ryadg, Rychc, Rysto), золотистой цистообразующей картофельной нематоде (H1, Gro1-4), бледной картофельной нематоде (Gpa2) с помощью генетических маркеров. В выборку вошли 2 сорта, 3 популяции, полученные от самоопыления сорта Идеал, и 35 индивидуально отобранных гиб ридов картофеля. В результате исследования маркер NL25 (ген Sen1) обнаружен у 19 образцов; маркер PVX (ген Rx) – у 13 об разцов; маркер RYSC3312 (ген Ryadg) – у 10 образцов; маркер YES3-3A341 (ген Rysto) – у 5 образцов; маркеры TG 689141, 57R450,  N195337(ген H1) – у 12 образцов; маркер Gro1-4-1602 (ген Gro1-4) –  у 6 образцов; маркер Gpa2-2452 (ген Gpa2) – у 13 образцов. По комплексу хозяйственно полезных признаков выделился образец С-31-15, обладающий высокими показателями урожайности и качества и являющийся носителем генетических маркеров устойчивости к вирусам X (Rx), Y (Rysto), золотистой цистообразующей картофельной нематоде (H1, Gro1-4), бледной нематоде (Gpa2).

The development of phytopathogen-resistant varieties is the most reliable and economic way to reduce potato yield losses. Breeding of such varieties is possible by using genetic sources of resistance. The use of DNA markers for identification of valuable genotypes, including forms with several resistance genes, makes it possible to significantly improve breeding efficiency. The development of a multiplex PCR technique and using it to simultaneously test varieties and breeding lines for several genes that control the resistance to viruses and nematodes is a new approach to using DNA markers. This study is aimed at screening samples from the collection of the Narym Department of  Breeding and Seed Production of the Siberian Research Institute of Agriculture and Peat (the Branch of the Siberian Federal Agrobiotechnology Research Center, the Russian Academy of Sciences) using the multiplex PCR technique, for genes for resistance to Globodera rostochiensis and Globodera pallidas, potato wart disease, viruses X and Y. 40 samples were tested by means of genetic markers to identify genes for resistance to potato wart disease (Sen1), virus X (Rx), virus Y (Ryadg, Rychc, Rysto), Globodera rostochiensis (H1, Gro1-4) and Globodera pallida (Gpa2), in the genome. The sample included two varieties, three populations produced by self-pollination of the Ideal variety, and 35 individually selected potato hybrids. As a result, we identified marker NL25 (Sen1) in 19 samples; marker PVX (Rx) in 13 samples; marker RYSC3312 (Ryadg) in 10 samples; marker YES3-3A341 (Rysto) in 5 samples; markers TG 689141, 57R450, N195337 (H1) in 12 samples; marker Gro1-4-1602 (Gro1-4) in 6 samples; marker Gpa2-2452 (Gpa2) in 13 samples. In terms of economically valuable traits, sample С-31-15 is noted for high yield and quality indicators. It carries genes for resistance to potato virus X (Rx), Y (Rysto), Globodera rostochiensis (H1, Gro1-4), and Globodera pallida (Gpa2).

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The authors declare that there are no conflicts of interest present.