vavilovВавиловский журнал генетики и селекцииVavilov Journal of Genetics and Breeding2500-3259Institute of Cytology and Genetics of Siberian Branch of the RAS10.18699/VJ18.336vavilov-1368Research ArticleГЕНЕТИЧЕСКИЕ РЕСУРСЫ РАСТЕНИЙPLANT GENETICS RESOUCESИЗУЧЕНИЕ ИНТРОГРЕССИВНЫХ ЛИНИЙ МЯГКОЙ ПШЕНИЦЫ С ГЕНЕТИЧЕСКИМ МАТЕРИАЛОМ Aegilops tauschii ПО УСТОЙЧИВОСТИ К ЛИСТОВОЙ РЖАВЧИНЕSTUDY OF INTROGRESSIVE LINES OF COMMON WHEAT WITH Aegilops tauschii GENETIC MATERIAL FOR RESISTANCE TO LEAF RUSTДавоянЭ. Р.DavoyanE. R.

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Krasnodar

davayan@rambler.ruМиковД. С.MikovD. S.

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ЗубановаЮ. С.ZubanovaY. S.

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БолдаковД. М.BoldakovD. M.

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ДавоянР. О.DavoyanR. O.

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БебякинаИ. В.BebyakinaI. V.

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БибишевВ. А.BibishevV. А.

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Национальный центр зерна им. П.П. ЛукьяненкоРоссияNational Center of Grain of P.P. LukyanenkoRussian Federation20182103201822197101Copyright © Давоян Э.Р., Миков Д.С., Зубанова Ю.С., Болдаков Д.М., Давоян Р.О., Бебякина И.В., Бибишев В.А., 20182018Давоян Э.Р., Миков Д.С., Зубанова Ю.С., Болдаков Д.М., Давоян Р.О., Бебякина И.В., Бибишев В.А.Davoyan E.R., Mikov D.S., Zubanova Y.S., Boldakov D.M., Davoyan R.O., Bebyakina I.V., Bibishev V.А.This work is licensed under a Creative Commons Attribution 4.0 License.https://vavilov.elpub.ru/jour/article/view/1368

Проведена оценка синтетических форм Тriticum miguschovae (GGAADD), Тriticum palmovae (AbAbDD), T. durum (M. it.)/Ae. Tauschii (BBAADD) и созданных на их основе интрогрессивных линий мягкой пшеницы на устойчивость к листовой ржавчине. Т. Miguschovae и Т. palmovae проявили полный иммунитет, симптомы поражения отсутствовали. Синтетическая форма T. durum (M. it.)/Ae. Tauschii проявляет высокую устойчивость. Отобраны 22 устойчивые к листовой ржавчине линии мягкой пшеницы с генетическим материалом T. miguschovae, 10 линий с генетическим материалом T. Durum (M. it.)/Ae. tauschii и 4 линии, полученные на основе Т. palmovaе. С использованием молекулярных маркеров проведен скрининг на присутствие генов устойчивости к листовой ржавчине Lr21, Lr26, Lr32, Lr39. Синтетические формы T. miguschovae, Т. palmovae, T. Durum (M. it.)/Ae. tauschii несут маркер GDM35, сцепленный с геном Lr39. Присутствие маркеров Lr21F/R и BARC135, сцепленных с генами Lr21 и Lr32 соответственно, не было выявлено. Показано, что устойчивость к листовой ржавчине в линиях 729, 1555, 2203, 2289, 2295, 2296, 4155, 4171, полученных на основе T. miguschovae, линиях 3261, 3265, полученных на основе Т. palmovaе, и в линии 4141 с генетическим материалом T. durum (M. it.)/Ae. tauschii контролируется присутствием гена Lr39. Маркер SCM9, указывающий на наличие транслокации 1BL.1RS с геном Lr26, наследуемой от сорта Кавказ, выявлен в 15 линиях, полученных на основе T. miguschovae, в двух линиях с генетическим материалом от T. durum (M. it.)/ Ae. tauschii и в одной линии, полученной с участием Т. palmovae. Линии 729, 1555, 2203, 2289, 2295, 2296, 4155, 4171, полученные с участием T. miguschovae, и линия 3261 с участием Т. palmovae несут комбинацию генов (Lr39+Lr26).

The synthetic forms of Triticum miguschovae (GGAADD), Triticum palmovae (AbAbDD), T. durum (M. it.)/Ae. Tauschii (BBAADD) and the introgressive lines of common wheat created on their basis were evaluated for resistance to leaf rust. All synthetic forms have a high resis­tance to leaf rust. Twenty-two lines of common wheatresistant to leaf rust with genetic material from T. miguschovae, 10 lines with genetic material from T. du­rum (M. it.)/Ae. tauschii and 4 lines obtained on the basis of T. palmovae were identified. A screening with the use of molecular markers for the presence of leaf rust resistance genes Lr21, Lr26, Lr32, Lr39 was done. The GDM35 marker linked to the Lr39 gene was identified in the synthetic forms. Molecular markers Lr21F/R and BARC135 linked to the genes Lr21 and Lr32, respectively, were not identified. Resistance to leaf rust in lines 729, 1555, 2203, 2289, 2295, 2296, 4155, 4171, obtained on the basis of T. miguschovae, lines 3261, 3265, obtained on the basis of T. palmovae, and in line 4141 with the genetic material from T. durum (M. it.)/Ae. Tauschii is controlled by the presence of the Lr39 gene. The SCM9 marker indicating the presence of translocation of 1BL.1RS with the Lr26 gene was detected in 15 lines obtained on the basis of T. miguschovae, in 2 lines with genetic material from T. durum (M. it.)/Ae. tauschii, and in 1 line created on the basis of T. palmovae. Lines 729, 1555, 2203, 2289, 2295, 2296, 4155, 4171 obtained with the participation of T. miguschovae and line 3261 with the participation of T. palmovae carry a combination of genes (Lr39+Lr26).

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The authors declare that there are no conflicts of interest present.