vavilovВавиловский журнал генетики и селекцииVavilov Journal of Genetics and Breeding2500-3259Institute of Cytology and Genetics of Siberian Branch of the RAS10.18699/VJ18.337vavilov-1369Research ArticleГЕНЕТИЧЕСКИЕ РЕСУРСЫ РАСТЕНИЙPLANT GENETICS RESOUCESМОЛЕКУЛЯРНО-ГЕНЕТИЧЕСКОЕ ИССЛЕДОВАНИЕ ОДУВАНЧИКА ОСЕННЕГО (Taraxacum hybernum Steven) С ИСПОЛЬЗОВАНИЕМ SSR-, RAPD- И ISSR-МАРКЕРОВTHE MOLECULAR GENETIC STUDY OF KRIM-SAGHYZ (Taraxacum hybernum Steven) USING SSR, RAPD AND ISSR MARKERSКулуевБ. Р.KuluevB. R.

Уфа

Ufa

kuluev@bk.ruФатерыгаА. В.FaterygaA. V.

Феодосия

Feodosiya

КулуевА. Р.KuluevA. R.

Уфа

Ufa

МихайловаЕ. В.MikhaylovaE. V.

Уфа

Ufa

ЧемерисА. В.ChemerisA. V.

Уфа

Ufa

Институт биохимии и генетики Уфимского научного центра РАНРоссияInstitute of Biochemistry and Genetics of Ufa Science Centre RASRussian FederationКарадагская научная станция им. Т.И. Вяземского – природный заповедник РАНРоссияT.I. Vyazemsky Karadag Scientific Station – Nature Reserve of RASRussian Federation201821032018221102107Copyright © Кулуев Б.Р., Фатерыга А.В., Кулуев А.Р., Михайлова Е.В., Чемерис А.В., 20182018Кулуев Б.Р., Фатерыга А.В., Кулуев А.Р., Михайлова Е.В., Чемерис А.В.Kuluev B.R., Fateryga A.V., Kuluev A.R., Mikhaylova E.V., Chemeris A.V.This work is licensed under a Creative Commons Attribution 4.0 License.https://vavilov.elpub.ru/jour/article/view/1369

Одуванчик осенний (Taraxacum hybernum Steven) – растение-каучуконос, одно из альтернативных гевее бразильской растений. В России произрастает только на Крымском полуострове, поэтому его часто называют крым-сагыз. Несмотря на свою потенциальную хозяйственную ценность, генетическая структура крымской популяции одуванчика осеннего на сегодняшний день остается неизученной. В связи с этим целью нашей работы была сравнительная молекулярно-генетическая характеристика одуванчика осеннего из различных местообитаний Крымского полуострова при использовании SSR-, RAPD- и ISSR-маркеров. В результате проведенной работы из десяти географических точек Крымского полуострова были собраны семянки, листья и корни одуванчика осеннего. В целом одуванчик осенний обнаружен нами в пределах западной части Южного берега Крыма и западной части Крымских Предгорий – двух основных регионов его произрастания на полуострове. Из сухих листьев анализируемых растений при помощи цетилтриметиламмоний бромида была выделена тотальная ДНК. Впервые на одуванчике осеннем были испытаны 12 SSR- и по три RAPD- и ISSR-маркера. Полиморфизм RAPD- и ISSR-фрагментов определяли аналитическим электрофорезом в 1.7 % агарозном геле. Для сравнительного анализа длин SSR-фрагментов использовали гель-электрофорез в 8 % полиакриламидном геле. В результате проведенной работы была показана гомогенность крымской популяции одуванчика осеннего, что может быть связано с небольшой областью распространения и апомиктичным способом размножения этого вида. Однако известные по литературным данным фенотипические различия внутри крымской популяции говорят о необходимости продолжения исследований полиморфизма ДНК одуванчика осеннего, в том числе с использованием высокоразрешающих методов анализа.

Krim-saghyz (Taraxacum hybernum Steven) is an alternative to Hevea brasiliensis as a source of natural rubber. In Russia, krim-saghyz is common only in the Crimean Peninsula and is traditionally named after it. In spite of its potential for economical use, the genetic structure of the Crimean population of this plant is still unexplored. In this regard, the purpose of our work was a comparative molecular-genetic characterization of T. hybernum from various habitats of the Crimean Peninsula using SSR, RAPD and ISSR markers. According to the plan, we collected achenes, leaves and roots of krim-saghyz in 10 spots all over the Crimean Peninsula. We found the plants in the western part of the southern Crimean coast and the western part of the Crimean foothills, which are two general regions of the area of this species. Total DNA was extracted from dry leaves of krim-saghyz with cetyltrimethylammonium bromide (CTAB). For the first time 12 SSR, 3 RAPD and 3 ISSR markers were tested on krim-saghyz. To observe polymorphism of RAPD- and ISSR-fragments, we used analytical electrophoresis in 1.7 % agarose gel. To compare the length of SSR amplicons, we used gel-electrophoresis in 8 % polyacrylamide gel. We found that the Crimean population of krim-saghyz appears to be genetically homogeneous. This could be due to a small geographic range and apomictic reproduction of this species. However, the phenotypical diversity within the population of T. hybernum is well known from the literature. Consequently, the study of the DNA polymorphism of this species should be continued, in particular, with the help of high-resolution techniques.

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The authors declare that there are no conflicts of interest present.