<?xml version="1.0" encoding="UTF-8"?>
<!DOCTYPE article PUBLIC "-//NLM//DTD JATS (Z39.96) Journal Publishing DTD v1.3 20210610//EN" "JATS-journalpublishing1-3.dtd">
<article article-type="research-article" dtd-version="1.3" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:lang="ru"><front><journal-meta><journal-id journal-id-type="publisher-id">vavilov</journal-id><journal-title-group><journal-title xml:lang="ru">Вавиловский журнал генетики и селекции</journal-title><trans-title-group xml:lang="en"><trans-title>Vavilov Journal of Genetics and Breeding</trans-title></trans-title-group></journal-title-group><issn pub-type="epub">2500-3259</issn><publisher><publisher-name>Institute of Cytology and Genetics of Siberian Branch of the RAS</publisher-name></publisher></journal-meta><article-meta><article-id custom-type="elpub" pub-id-type="custom">vavilov-151</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="ru"><subject>Статьи</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="en"><subject>Articles</subject></subj-group></article-categories><title-group><article-title>ИЗУЧЕНИЕ АЛЛЕЛЬНОГО РАЗНООБРАЗИЯ ГЕНОВ СИНТЕЗА ЭТИЛЕНА MD-ACS1 И MD-ACO1 В ОТЕЧЕСТВЕННОЙ ГЕНПЛАЗМЕ ЯБЛОНИ</article-title><trans-title-group xml:lang="en"><trans-title>ALLELIC DIVERSITY OF ETHYLENE BIOSYNTHESIS-RELATED MD-ACS1 AND MD-ACO1 GENES IN RUSSIAN APPLE GERMPLASM</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Супрун</surname><given-names>И. И.</given-names></name><name name-style="western" xml:lang="en"><surname>Suprun</surname><given-names>I. I.</given-names></name></name-alternatives><email xlink:type="simple">supruni@mail.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Токмаков</surname><given-names>С. В.</given-names></name><name name-style="western" xml:lang="en"><surname>Tokmakov</surname><given-names>S. V.</given-names></name></name-alternatives><email xlink:type="simple">supruni@mail.ru</email><xref ref-type="aff" rid="aff-1"/></contrib></contrib-group><aff-alternatives id="aff-1"><aff xml:lang="ru">Федеральное государственное бюджетное научное учреждение "Северо-Кавказский зональный научно-исследовательский институт садоводства и виноградарства", Краснодар, Россия<country>Россия</country></aff><aff xml:lang="en">North-Caucasian Zonal Research and Development Institute of Horticulture and Viticulture, Krasnodar, Russia<country>Russian Federation</country></aff></aff-alternatives><pub-date pub-type="collection"><year>2013</year></pub-date><pub-date pub-type="epub"><day>26</day><month>12</month><year>2014</year></pub-date><volume>17</volume><issue>2</issue><fpage>298</fpage><lpage>302</lpage><permissions><copyright-statement>Copyright &amp;#x00A9; Супрун И.И., Токмаков С.В., 2014</copyright-statement><copyright-year>2014</copyright-year><copyright-holder xml:lang="ru">Супрун И.И., Токмаков С.В.</copyright-holder><copyright-holder xml:lang="en">Suprun I.I., Tokmakov S.V.</copyright-holder><license license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>This work is licensed under a Creative Commons Attribution 4.0 License.</license-p></license></permissions><self-uri xlink:href="https://vavilov.elpub.ru/jour/article/view/151">https://vavilov.elpub.ru/jour/article/view/151</self-uri><abstract><p>В статье приведены результаты изучения аллельного разнообразия генов синтеза этилена Md-ACS1 и Md-ACO1 в отечественной генплазме яблони с применением ДНК-маркерного анализа. Идентифицированы аллельные комбинации данных генов у 48 сортов яблони отечественной селекции. Выявлено, что уровень распространения аллельных комбинаций, обусловливающих высокую степень лежкости плодов, в отечественной генплазме соответствует частоте их встречаемости в мировом генофонде культуры. С учетом полученных данных начата программа по созданию селекционных форм яблони, несущих сочетание аллелей Md-ACS1-2/2 + Md-ACО1-1/1, с применением ДНК-маркерного отбора для селекции сортов, обладающих наиболее высокой степенью лежкости плодов.</p></abstract><trans-abstract xml:lang="en"><p>The allelic diversity of the Md-ACS1 and Md-ACO1 genes, involved in ethylene biosynthesis, was studied by DNA marker analysis of 48 apple varieties bred in Russia. Different allelic combinations of these genes were identified. We found that the prevalence of the allelic combinations associated with long shelf life of apple fruit in the Russian germplasm corresponded to the occurrence of these alleles in the worldwide gene pool. With regard to these data, a marker-assisted breeding program was launched to develop forms carrying the Md-ACS1-2/2 + Md-ACO1-1/1 allele combination for long fruit shelf life.</p></trans-abstract><kwd-group xml:lang="ru"><kwd>яблоня</kwd><kwd>ДНК-маркерный анализ</kwd><kwd>маркер-вспомогательная селекция</kwd><kwd>аллельное разнообразие</kwd><kwd>гены синтеза этилена Md-ACS1 и Md-ACO1</kwd><kwd>лежкость плодов</kwd></kwd-group><kwd-group xml:lang="en"><kwd>apple</kwd><kwd>DNA marker analysis</kwd><kwd>marker-assisted selection</kwd><kwd>allelic diversity</kwd><kwd>ethylene biosynthesis genes</kwd><kwd>Md-ACS1</kwd><kwd>Md-ACO1</kwd><kwd>fruit storability</kwd></kwd-group><funding-group xml:lang="ru"><funding-statement>Росский фонд фундаментальных исследований, проект № 12-04-31947 мол_а</funding-statement></funding-group></article-meta></front><back><ref-list><title>References</title><ref id="cit1"><label>1</label><citation-alternatives><mixed-citation xml:lang="ru">Adams D.O., Yang S.F. Ethylene biosynthesis: identification of 1-aminocyclopropane-1-carboxylic acid as an intermediate in the conversion of methionine to ethylene // Proc. Natl Acad. Sci. USA. 1979. V. 76. P. 170–174.</mixed-citation><mixed-citation xml:lang="en">Adams D.O., Yang S.F. Ethylene biosynthesis: identification of 1-aminocyclopropane-1-carboxylic acid as an intermediate in the conversion of methionine to ethylene // Proc. Natl Acad. Sci. USA. 1979. V. 76. P. 170–174.</mixed-citation></citation-alternatives></ref><ref id="cit2"><label>2</label><citation-alternatives><mixed-citation xml:lang="ru">Costa F., Sara S., Van de Weg W.E. et al. Role of the genes Md-ACO1 and Md-ACS1 in ethylene production and shelf life of apple (Malus domestica Borkh) // Euphytica. 2005. V. 141. P. 181–190.</mixed-citation><mixed-citation xml:lang="en">Costa F., Sara S., Van de Weg W.E. et al. Role of the genes Md-ACO1 and Md-ACS1 in ethylene production and shelf life of apple (Malus domestica Borkh) // Euphytica. 2005. V. 141. P. 181–190.</mixed-citation></citation-alternatives></ref><ref id="cit3"><label>3</label><citation-alternatives><mixed-citation xml:lang="ru">Dong J.G., Kim W.T, Yip W.K. et al. Cloning of a cDNA encoding 1-aminocyclopropane-1-carboxylate synthase and expression of its mRNA in ripening apple fruit // Planta. 1991. V. 185. P. 38–45.</mixed-citation><mixed-citation xml:lang="en">Dong J.G., Kim W.T, Yip W.K. et al. Cloning of a cDNA encoding 1-aminocyclopropane-1-carboxylate synthase and expression of its mRNA in ripening apple fruit // Planta. 1991. V. 185. P. 38–45.</mixed-citation></citation-alternatives></ref><ref id="cit4"><label>4</label><citation-alternatives><mixed-citation xml:lang="ru">Dong J.G., Olson D., Silverstone A., Yang S.F. Sequence of a cDNA coding for a 1-aminocyclopropane-1-carboxylate oxidase homolog from apple fruit // Plant Physiol. 1992. V. 98. P. 1530–1531.</mixed-citation><mixed-citation xml:lang="en">Dong J.G., Olson D., Silverstone A., Yang S.F. Sequence of a cDNA coding for a 1-aminocyclopropane-1-carboxylate oxidase homolog from apple fruit // Plant Physiol. 1992. V. 98. P. 1530–1531.</mixed-citation></citation-alternatives></ref><ref id="cit5"><label>5</label><citation-alternatives><mixed-citation xml:lang="ru">Fan X., Blankenship S.M., Mattheis J.P. 1-methylcyclopropene inhibits apple ripening // J. Am. Soc. Hortic. Sci. 1999. V. 124. P. 690–695.</mixed-citation><mixed-citation xml:lang="en">Fan X., Blankenship S.M., Mattheis J.P. 1-methylcyclopropene inhibits apple ripening // J. Am. Soc. Hortic. Sci. 1999. V. 124. P. 690–695.</mixed-citation></citation-alternatives></ref><ref id="cit6"><label>6</label><citation-alternatives><mixed-citation xml:lang="ru">Harada T., Sunako T., Wakasa Y. et al. An allele of 1-aminocyclopropane-1-carboxylate synthase gene (Md-ACS1) accounts for the low ethylene production in climacteric fruits of some apple cultivars // Theor. Appl. Genet. 2000. V. 101. P. 742–746.</mixed-citation><mixed-citation xml:lang="en">Harada T., Sunako T., Wakasa Y. et al. An allele of 1-aminocyclopropane-1-carboxylate synthase gene (Md-ACS1) accounts for the low ethylene production in climacteric fruits of some apple cultivars // Theor. Appl. Genet. 2000. V. 101. P. 742–746.</mixed-citation></citation-alternatives></ref><ref id="cit7"><label>7</label><citation-alternatives><mixed-citation xml:lang="ru">Kende H. Ethylene biosynthesis // Annu. Rev. Plant Physiol. Plant Mol. Biol. 1993. V. 44. P. 283–307.</mixed-citation><mixed-citation xml:lang="en">Kende H. Ethylene biosynthesis // Annu. Rev. Plant Physiol. Plant Mol. Biol. 1993. V. 44. P. 283–307.</mixed-citation></citation-alternatives></ref><ref id="cit8"><label>8</label><citation-alternatives><mixed-citation xml:lang="ru">Mercantila F. Guide to Food Transport: Fruit and Vegetables. Copenhagen: Mercantila Publishers, 1989. 247 p.</mixed-citation><mixed-citation xml:lang="en">Mercantila F. Guide to Food Transport: Fruit and Vegetables. Copenhagen: Mercantila Publishers, 1989. 247 p.</mixed-citation></citation-alternatives></ref><ref id="cit9"><label>9</label><citation-alternatives><mixed-citation xml:lang="ru">Murray M.G., Thompson W.F. Rapid isolation of high molecular weight plant DNA // Nucl. Acids Res. 1980. V. 10. P. 4321–4325.</mixed-citation><mixed-citation xml:lang="en">Murray M.G., Thompson W.F. Rapid isolation of high molecular weight plant DNA // Nucl. Acids Res. 1980. V. 10. P. 4321–4325.</mixed-citation></citation-alternatives></ref><ref id="cit10"><label>10</label><citation-alternatives><mixed-citation xml:lang="ru">Nybom H., Ahmadi-Afzadi M., Sehic J., Hertog M. DNA marker-assisted evaluation of fruit fi rmness at harvest and post-harvest fruit softening in a diverse apple germplasm // Tree Genetics and Genomes. 2013. V. 9. P. 279–290.</mixed-citation><mixed-citation xml:lang="en">Nybom H., Ahmadi-Afzadi M., Sehic J., Hertog M. DNA marker-assisted evaluation of fruit fi rmness at harvest and post-harvest fruit softening in a diverse apple germplasm // Tree Genetics and Genomes. 2013. V. 9. P. 279–290.</mixed-citation></citation-alternatives></ref><ref id="cit11"><label>11</label><citation-alternatives><mixed-citation xml:lang="ru">Oraguzie N.C., Iwanami H., Soejima J. et al. A. Inheritance of Md-ACS1 gene and its relationship to fruit softening in apple (Malus × domestica Borkh.) // Theor. Appl. Genet. 2004. V. 108. P. 1526–1533.</mixed-citation><mixed-citation xml:lang="en">Oraguzie N.C., Iwanami H., Soejima J. et al. A. Inheritance of Md-ACS1 gene and its relationship to fruit softening in apple (Malus × domestica Borkh.) // Theor. Appl. Genet. 2004. V. 108. P. 1526–1533.</mixed-citation></citation-alternatives></ref><ref id="cit12"><label>12</label><citation-alternatives><mixed-citation xml:lang="ru">Sunako T., Sakuraba W., Senda M. et al. An allele of the ripening-specifi c 1-aminocyclopropane-1-carboxylic acid synthase gene (ACS1) in apple fruit with a long storage life // Plant Physiol. 1999. V. 119. P. 1297–1304.</mixed-citation><mixed-citation xml:lang="en">Sunako T., Sakuraba W., Senda M. et al. An allele of the ripening-specifi c 1-aminocyclopropane-1-carboxylic acid synthase gene (ACS1) in apple fruit with a long storage life // Plant Physiol. 1999. V. 119. P. 1297–1304.</mixed-citation></citation-alternatives></ref><ref id="cit13"><label>13</label><citation-alternatives><mixed-citation xml:lang="ru">Tucker G.A. Introduction // Biochemistry of Fruit Ripening / Eds G.B. Seymour, J.E. Taylor, G.A. Tucker. London: Chapman and Hall, 1993. P. 3–51.</mixed-citation><mixed-citation xml:lang="en">Tucker G.A. Introduction // Biochemistry of Fruit Ripening / Eds G.B. Seymour, J.E. Taylor, G.A. Tucker. London: Chapman and Hall, 1993. P. 3–51.</mixed-citation></citation-alternatives></ref><ref id="cit14"><label>14</label><citation-alternatives><mixed-citation xml:lang="ru">Wiersma P.A., Zhang H., Lu C. et al. Survey of the expression of genes for ethylene synthesis and perception during maturation and ripening of «Sunrise» and «Golden Delicious » apple fruit // Postharvest Biol. Technol. 2007. V. 44. No. 3. P. 204–211.</mixed-citation><mixed-citation xml:lang="en">Wiersma P.A., Zhang H., Lu C. et al. Survey of the expression of genes for ethylene synthesis and perception during maturation and ripening of «Sunrise» and «Golden Delicious » apple fruit // Postharvest Biol. Technol. 2007. V. 44. No. 3. P. 204–211.</mixed-citation></citation-alternatives></ref><ref id="cit15"><label>15</label><citation-alternatives><mixed-citation xml:lang="ru">Zhu Y., Barrit B.H. Md-ACS1 and Md-ACO1 genotyping of apple (Malus × domestica Borkh.) breeding parents and suitability for marker-assisted selection // Tree Genetics and Genomes. 2008. V. 4. P. 555–562.</mixed-citation><mixed-citation xml:lang="en">Zhu Y., Barrit B.H. Md-ACS1 and Md-ACO1 genotyping of apple (Malus × domestica Borkh.) breeding parents and suitability for marker-assisted selection // Tree Genetics and Genomes. 2008. V. 4. P. 555–562.</mixed-citation></citation-alternatives></ref></ref-list><fn-group><fn fn-type="conflict"><p>The authors declare that there are no conflicts of interest present.</p></fn></fn-group></back></article>
