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<article article-type="research-article" dtd-version="1.3" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:lang="ru"><front><journal-meta><journal-id journal-id-type="publisher-id">vavilov</journal-id><journal-title-group><journal-title xml:lang="ru">Вавиловский журнал генетики и селекции</journal-title><trans-title-group xml:lang="en"><trans-title>Vavilov Journal of Genetics and Breeding</trans-title></trans-title-group></journal-title-group><issn pub-type="epub">2500-3259</issn><publisher><publisher-name>Institute of Cytology and Genetics of Siberian Branch of the RAS</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.18699/VJ18.368</article-id><article-id custom-type="elpub" pub-id-type="custom">vavilov-1511</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="ru"><subject>ГЕНОМИКА И ТРАНСКРИПТОМИКА</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="en"><subject>PLANT GENETICS AND BREEDING</subject></subj-group></article-categories><title-group><article-title>ИДЕНТИФИКАЦИЯ ГЕНА УСТОЙЧИВОСТИ К БУРОЙ РЖАВЧИНЕ Lr34  В СОРТАХ И КОЛЛЕКЦИОННЫХ ОБРАЗЦАХ  ОЗИМОЙ МЯГКОЙ ПШЕНИЦЫ АГРАРНОГО НАУЧНОГО ЦЕНТРА «ДОНСКОЙ»</article-title><trans-title-group xml:lang="en"><trans-title>IDENTIICATION OF THE Lr34 GENE FOR RESISTANCE TO LEAF RUST IN VARIETIES AND COLLECTION SAMPLES OF WINTER SOFT WHEAT FROM THE AGRICULTURAL RESEARCH CENTER “DONSKOY”</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Вожжова</surname><given-names>Н. Н.</given-names></name><name name-style="western" xml:lang="en"><surname>Vozhzhova</surname><given-names>N. N.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Ростовская область, Зерноград</p></bio><bio xml:lang="en"><p>Rostov region, Zernograd</p></bio><email xlink:type="simple">nvozhzh@gmail.com</email><xref ref-type="aff" rid="aff-1"/></contrib></contrib-group><aff-alternatives id="aff-1"><aff xml:lang="ru">Аграрный научный центр «Донской»<country>Россия</country></aff><aff xml:lang="en">Agricultural Research Center “Donskoy”<country>Russian Federation</country></aff></aff-alternatives><pub-date pub-type="collection"><year>2018</year></pub-date><pub-date pub-type="epub"><day>24</day><month>05</month><year>2018</year></pub-date><volume>22</volume><issue>3</issue><fpage>329</fpage><lpage>332</lpage><permissions><copyright-statement>Copyright &amp;#x00A9; Вожжова Н.Н., 2018</copyright-statement><copyright-year>2018</copyright-year><copyright-holder xml:lang="ru">Вожжова Н.Н.</copyright-holder><copyright-holder xml:lang="en">Vozhzhova N.N.</copyright-holder><license license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>This work is licensed under a Creative Commons Attribution 4.0 License.</license-p></license></permissions><self-uri xlink:href="https://vavilov.elpub.ru/jour/article/view/1511">https://vavilov.elpub.ru/jour/article/view/1511</self-uri><abstract><p>Одно из приоритетных направлений селекции озимой мягкой пшеницы в настоящее время – обеспечение сопротивляемости листовым заболеваниям и, в частности, бурой ржавчине как самой вредоносной из них. Ген устойчивости к бурой ржавчине, Lr34, обеспечивает резистентность растений пшеницы по типу медленного развития. Использование Lr34 в сочетании с другими генами устойчивости к бурой ржавчине (Lr2c, Lr10, Lr12, Lr13, Lr16 и др.) позволит получить образцы и сорта, менее подверженные болезни в полевых условиях. Целью работы была идентификация гена Lr34 в сортах и коллекционных образцах озимой мягкой пшеницы ФГБНУ «Аграрный научный центр «Донской» (АНЦ «Донской»), поскольку предполагалось его присутствие благодаря вовлечению в гибридизацию в 1960­е гг. сорта Безостая 1, а изучение этого гена в нашем коллекционном материале ранее не проводилось. В статье представлены результаты работы по идентификации у 646 образцов озимой мягкой пшеницы (в том числе 36 сортов АНЦ «Донской», внесенных в Государственный реестр селекционных достижений, допущенных к использованию, и 8 сортов, проходящих государственное сортоиспытание) гена устойчивости к бурой ржавчине Lr34 и его аллельного состояния. Оценка аллелей гена Lr34 проводилась при помощи кодоминантного молекулярного STS­маркера csLV34 методом полимеразной цепной реакции. Всего было идентифицировано 238 образцов, несущих ген устойчивости к бурой ржавчине Lr34 в доминантном аллельном состоянии. Сорта селекции АНЦ «Донской» Находка, Бонус, Конкурент, Аксинья, Казачка, Донская полукарликовая, Донская безостая, Изюминка, Зерноградка 9, Зерноградка 10 и Зерноградка 11 рекомендуются в качестве источников гена Lr34 для селекции на устойчивость озимой мягкой пшеницы к болезням сельскохозяйственных культур, поскольку несут его доминантный аллель.</p></abstract><trans-abstract xml:lang="en"><p>Nowadays one of the priorities in winter soft wheat breeding is to provide resistance to leaf diseases, and, in particular, to leaf rust as the most harmful of them. The gene for resistance to leaf rust, Lr34, provides resistance to wheat plants at the slow development. The use of this gene together with other genes for resistance to leaf rust will allow obtaining varieties and samples less susceptible to the disease. The purpose of this work was to identify the Lr34 gene in the  varieties and collection samples of winter soft wheat in the Agricultural Research Center “Donskoy” (ARC “Donskoy”), because its presence was suspected due to the introduction of the variety “Bezostaya 1” in hybridization of the 1960s, but the study of this gene in our collection has not been carried out yet. The article gives the results of the study of 646 samples of winter soft wheat (including 36 varieties of ARC “Donskoy” introduced in the State Register of Breeding Achievements of the Russian Federation and 8 varieties being tested in the State Variety Testing) and identiication of the Lr34 gene and its allelic state. The assessment of the allelic state of the Lr34 gene has been carried out with the help of the codominant marker csLV34 using the method of PCR. There have been identiied 238 samples with the Lr34 gene in the dominant allelic state. The varieties Nakhodka, Bonus, Konkurent, Aksiniya, Kazachka, Donskaya polukarlikovaya, Donskaya bezostaya, Izyuminka, Zernogradka 9, Zernogradka 10 and Zernogradka 11 developed in SSE “ARC “Donskoy” are recommended as a source of the Lr34 gene to breed winter soft wheat resistant to the diseases.</p></trans-abstract><kwd-group xml:lang="ru"><kwd>сорта</kwd><kwd>образцы</kwd><kwd>озимая мягкая пшеница</kwd><kwd>бурая ржавчина</kwd><kwd>ген</kwd><kwd>устойчивость</kwd><kwd>Lr34</kwd><kwd>полимеразная цепная реакция</kwd><kwd>идентификация</kwd></kwd-group><kwd-group xml:lang="en"><kwd>varieties</kwd><kwd>samples</kwd><kwd>winter soft wheat</kwd><kwd>leaf rust</kwd><kwd>gene</kwd><kwd>resistance</kwd><kwd>Lr34</kwd><kwd>polymerase chain reaction</kwd><kwd>identiication</kwd></kwd-group><funding-group xml:lang="en"><funding-statement>Genotyping for the Lr34 gene for leaf rust resistance in winter wheat varieties and accessions was supported by State Budgeted Project 0706-2015-0001; Head of the Department of Winter Wheat Breeding and Seed Industry of Agrarian Research Center Donskoy D.M. Marchenko for kindly provided material for the study</funding-statement></funding-group></article-meta></front><back><ref-list><title>References</title><ref id="cit1"><label>1</label><citation-alternatives><mixed-citation xml:lang="ru">Brody J.R., Calhoun E.S., Gallmeier E., Creavalle T.D., Kern S.E. Ultrafast high-resolution agarose electrophoresis of DNA and RNA using low-molarity conductive media. Biotechniques. 2004;37(4):598-602.</mixed-citation><mixed-citation xml:lang="en">Brody J.R., Calhoun E.S., Gallmeier E., Creavalle T.D., Kern S.E. Ultrafast high-resolution agarose electrophoresis of DNA and RNA using low-molarity conductive media. Biotechniques. 2004;37(4):598-602.</mixed-citation></citation-alternatives></ref><ref id="cit2"><label>2</label><citation-alternatives><mixed-citation xml:lang="ru">Gultyaeva E.I., Sadovaya A.S. Breeding of soft wheat for resistance to brown rust in Russia. Zashchita i Karantin Rasteniy = Plant Protection and Quarantine. 2014;10:24-26. 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