vavilovВавиловский журнал генетики и селекцииVavilov Journal of Genetics and Breeding2500-3259Institute of Cytology and Genetics of Siberian Branch of the RAS10.18699/VJ18.375vavilov-1542Research ArticleФИЗИОЛОГИЧЕСКАЯ ГЕНЕТИКАPHYSIOLOGICAL GENETICSСПЕРМАТОГЕННАЯ ФУНКЦИЯ СЕМЕННИКОВ У КРЫС С НАСЛЕДСТВЕННОЙ ПРЕДРАСПОЛОЖЕННОСТЬЮ К ПРОЯВЛЕНИЮ КАТАТОНИЧЕСКИХ РЕАКЦИЙSPERM QUALITY IN RATS PREDISPOSED TO THE MANIFESTATION OF CATATONIC REACTIONSКлещевМ. А.KleshchevM. A.

Новосибирск

Novosibirsk

max82cll@bionet.nsc.ruАлехинаТ. А.AlekhinaT. A.

Новосибирск

Novosibirsk

ОсадчукЛ. В.OsadchukL. V.

Новосибирск

Novosibirsk

Федеральный исследовательский центр Институт цитологии и генетики Сибирского отделения Российской академии наукРоссияInstitute of Cytology and Genetics SB RASRussian Federation201803072018224400405Copyright © Клещев М.А., Алехина Т.А., Осадчук Л.В., 20182018Клещев М.А., Алехина Т.А., Осадчук Л.В.Kleshchev M.A., Alekhina T.A., Osadchuk L.V.This work is licensed under a Creative Commons Attribution 4.0 License.https://vavilov.elpub.ru/jour/article/view/1542

Кататония – психопатологический синдром, проявляющийся как двигательные расстройства. Кататонический синдром сопутствует многим психическим заболеваниям, в частности шизофрении и депрессии, которые широко распространены в популяции человека. Линия крыс ГК (генетическая кататония) получена из линии Вистар путем длительной (78 поколений) селекции на кататонический тип реагирования и служит моделью шизофренных и депрессивных состояний у человека. Известно, что селекция по поведению, в том числе по выраженности кататонической реакции, при водит к нейроэндокринным, репродуктивным и морфологическим изменениям у животных. Однако влияние отбора по выраженности кататонической реакции на сперматогенную функцию  семенников у самцов крыс не изучалось. Целью настоящей работы было провести сравнительное исследование сперматогенной функции се менников у крыс линии ГК и линии Вистар в период полового созревания (50­й день жизни) и у взрослых животных (90­й день жизни). Определяли количество эпидидимальных сперматозои дов, долю половых клеток с прогрессивным движением, долю морфологически аномальных сперматозоидов, а также массу репродуктивных органов и размер помета. У взрослых крыс линии ГК количество сперматозоидов и их подвижность, масса тела, семенников и каудальных эпидидимисов, количество рожденных потомков были снижены по сравнению с крысами линии Вистар. В период полового созревания крысы линии ГК характеризовались бóльшим количеством сперматозоидов по сравнению с линией Вистар. Межлинейных различий по доле морфологических аномалий сперматозоидов у самцов крыс не отмечено. Предполагается, что изменения сперматогенных показателей при селекции крыс на выраженность кататонической реакции обусловлены нарушением онтогенетического паттерна секреции тестостерона. Таким образом, наследственная предрасположенность к проявлению кататонических реакций может быть ассоциирована с ухудшением сперматогенных параметров половозрелых самцов крыс. Линия крыс ГК может являться перспективной моделью для исследования взаимосвязи между наследственной предрасположенностью к кататонии и сперматогенезом.

Catatonia is a psychopathological syndrome displayed as a motor disorder. Catatonia is a sign of many menthal disorders, particularly schizophrenia and depression, with a wide disrtibution in the human population. The GC (“genetic” and “catatonia”) rat strain was obtained from the Wistar rat strain by a long selection (78 generations) for the catatonic type of reaction and is a model of schizophrenic and depressive disorders in humans. It is known that selection for behavior including catatonic reactions results in neuroendocrine, reproductive and morphological changes in animals. However, the influence of selection for a catatonic reaction on the spermatogenic function of testes had not been studied. The aim of this study was to conduct a comparative investigation of sperm quality in rats of the GC and the Wistar strain. The epididymal sperm parameters (sperm count, sperm motility, sperm morphology) were measured, and body, testes and epididymal weight were determined at puberty (50 day of life) and at adulthood (90 day of life). The litter size of the GC and Wistar rats was determined. It was found that adult GC rats had a lower sperm count, sperm motility, testis weight, epydidymal weight and litter size compared to adult Wistar rats. However, at puberty, GC rats had a higher sperm count than the Wistar strain. Interstrain differences in sperm morphology were not found. It has been assumed that the changes of spermatogenic parameters in response to selection for catatonia are caused by changing the ontogenic pattern of testosterone secretion. In conclusion, the hereditary predisposition to catatonic reaction is associated with impaired sperm parameters in adult rats that reduces their chance to reproduction. The GC rat strain can be a perspective model for investigation of the relationship between the hereditary predisposition to catatonia and spermatogenesis

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The authors declare that there are no conflicts of interest present.