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<article article-type="research-article" dtd-version="1.3" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:lang="ru"><front><journal-meta><journal-id journal-id-type="publisher-id">vavilov</journal-id><journal-title-group><journal-title xml:lang="ru">Вавиловский журнал генетики и селекции</journal-title><trans-title-group xml:lang="en"><trans-title>Vavilov Journal of Genetics and Breeding</trans-title></trans-title-group></journal-title-group><issn pub-type="epub">2500-3259</issn><publisher><publisher-name>Institute of Cytology and Genetics of Siberian Branch of the RAS</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.18699/VJ18.389</article-id><article-id custom-type="elpub" pub-id-type="custom">vavilov-1584</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="ru"><subject>ГЕНЕТИЧЕСКИЕ РЕСУРСЫ И БИОКОЛЛЕКЦИИ</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="en"><subject>PLANT GENETICS</subject></subj-group></article-categories><title-group><article-title>Изучение генетического полиморфизма диплоидной пшеницы Triticum boeoticum Boiss. с использованием SSR-маркеров</article-title><trans-title-group xml:lang="en"><trans-title>Study of the genetic polymorphism of diploid wheat Triticum boeoticum Boiss. using SSR markers</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Аббасов</surname><given-names>М. А.</given-names></name><name name-style="western" xml:lang="en"><surname>Abbasov</surname><given-names>M. A.</given-names></name></name-alternatives><bio xml:lang="ru"/><bio xml:lang="en"/><email xlink:type="simple">mehraj_genetic@yahoo.com</email><xref ref-type="aff" rid="aff-1"/></contrib></contrib-group><aff-alternatives id="aff-1"><aff xml:lang="ru">Институт генетических ресурсов Национальной академии наук Азербайджана<country>Азербайджан</country></aff><aff xml:lang="en">Genetic Resources Institute of Azerbaijan National Academy of Sciences<country>Azerbaijan</country></aff></aff-alternatives><pub-date pub-type="collection"><year>2018</year></pub-date><pub-date pub-type="epub"><day>08</day><month>08</month><year>2018</year></pub-date><volume>22</volume><issue>5</issue><fpage>515</fpage><lpage>523</lpage><permissions><copyright-statement>Copyright &amp;#x00A9; Аббасов М.А., 2018</copyright-statement><copyright-year>2018</copyright-year><copyright-holder xml:lang="ru">Аббасов М.А.</copyright-holder><copyright-holder xml:lang="en">Abbasov M.A.</copyright-holder><license license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>This work is licensed under a Creative Commons Attribution 4.0 License.</license-p></license></permissions><self-uri xlink:href="https://vavilov.elpub.ru/jour/article/view/1584">https://vavilov.elpub.ru/jour/article/view/1584</self-uri><abstract><p>Диплоидная пшеница Triticum boeoticum Boiss. (геном АА) – потен­циальный источник новых ценных аллелей для улучшения возде­лываемых видов пшеницы. В связи с этим оценка внутривидового разнообразия T. boeoticum и ДНК-паспортизация образцов этого вида является актуальной задачей. В настоящей работе исследо­вано генетическое разнообразие более 60 образцов T. boeoticum с использованием 11 микросателлитных маркеров. По данным SSR-анализа было идентифицировано 83 аллеля, в среднем на­блюдалось по 7.5 аллелей на локус. Величины ожидаемой (HE) и наблюдаемой (HO) гетерозиготности варьировали в пределах 0.17–0.89 и 0.00–0.74 при среднем показателе HE = 0.52 и HO = 0.13 соответственно. Значение PIC для каждого локуса находилось в пределах 0.17–0.88 и в среднем равнялось 0.49. Для всех изучен­ных локусов были обнаружены уникальные аллели. Кластерный анализ позволил объединить изученные образцы в пять основных групп, расстояния между группами варьировали от 0 до 1, что указывает на высокий уровень генетических различий в исследу­емой коллекции. Согласно анализу PCoA, было образовано пять основных групп и выявлены некоторые соответствия с дендро­граммой. При обобщении полученных данных PCoA и кластерного анализа отмечена слабая генетическая дифференциация изучен­ной коллекции T. boeoticum. Корреляция генетического расстояния с географическим происхождением выявлена лишь для образцов диплоидной пшеницы T. boeoticum из Ирана. Анализ образцов показывает широкое разнообразие T. boeoticum по микросателлит­ным локусам. Полученные нами данные расширяют представле­ния и дают дополнительную информацию о генетической структу­ре коллекции и разнообразии изученных образцов T. boeoticum.</p></abstract><trans-abstract xml:lang="en"><p>Diploid wheat Triticum boeoticum Boiss. (genome constitution AA) is a promising source of new valu­able alleles for improving cultivated wheat species. Therefore, the evaluation of the intraspecies diversity of T. boeoticum and DNA fingerprinting of accessions of this species are topical tasks. In this paper, the ge­netic diversity of over 60 T. boeoticum accessions was studied using 11 SSR markers. The analysis revealed 83 alleles, 7.5 alleles per locus on the average. The values of expected (HE) and observed (HO) heterozygosity varied within 0.00–0.74 and 0.17–0.89, respectively, the average indices being HO = 0.13 and HE = 0.52. The PIC value for each locus was within 0.17–0.88, 0.49 on the average. Unique alleles were found in all loci studied. Cluster analysis allowed the accessions studied to be combined into five major groups. The distances between the groups varied from 0 to 1, pointing to a high level of genetic differences in the collection under study. On the base of PCoA, five major groups were formed and some correspondence with the dendrogram was detected. Summarizing the data of PCoA and cluster analysis, we noted a weak genetic differentiation in the studied collection of T. boeoticum. A correlation between the genetic distance and geographic origin was revealed only for accessions of diploid wheat T. boeoticum from Iran. The analysis of the T. boeoticum accessions studied showed a wide diversity for SSR loci. The results expand our knowledge and pro­vide additional information on the genetic structure of the collection and on the genetic diversity of T. boeoti­cum accessions studied.</p></trans-abstract><kwd-group xml:lang="ru"><kwd>T. boeoticum</kwd><kwd>генетический полиморфизм</kwd><kwd>SSR-маркеры</kwd><kwd>кластерный анализ</kwd><kwd>анализ PCoA</kwd></kwd-group><kwd-group xml:lang="en"><kwd>T. boeoticum</kwd><kwd>genetic polymorphism</kwd><kwd>SSR markers</kwd><kwd>cluster analysis</kwd><kwd>principal coordinates analysis</kwd></kwd-group></article-meta></front><back><ref-list><title>References</title><ref id="cit1"><label>1</label><citation-alternatives><mixed-citation xml:lang="ru">Abouzied H.M., Eldemery S.M.M., Abdellatif K.F. SSR-based genetic diversity assessement in tetraploid and hexaploid wheat populations. Br. Biotechnol. J. 2013;3:390-404.</mixed-citation><mixed-citation xml:lang="en">Abouzied H.M., Eldemery S.M.M., Abdellatif K.F. SSR-based genetic diversity assessement in tetraploid and hexaploid wheat populations. Br. Biotechnol. J. 2013;3:390-404.</mixed-citation></citation-alternatives></ref><ref id="cit2"><label>2</label><citation-alternatives><mixed-citation xml:lang="ru">Aliyev R.T., Abbasov M.A., Mammadov A.C. Genetic identification of diploid and tetraploid wheat species with RAPD markers. Turk. J. Biol. 2007;31(3):173-180.</mixed-citation><mixed-citation xml:lang="en">Aliyev R.T., Abbasov M.A., Mammadov A.C. Genetic identification of diploid and tetraploid wheat species with RAPD markers. Turk. J. Biol. 2007;31(3):173-180.</mixed-citation></citation-alternatives></ref><ref id="cit3"><label>3</label><citation-alternatives><mixed-citation xml:lang="ru">Anker C.C., Niks R.E. Prehaustorial resistance to the wheat leaf rust fungus, Puccinia triticina, in Triticum monococcum (s.s.). Euphytica. 2001;117:3-12.</mixed-citation><mixed-citation xml:lang="en">Anker C.C., Niks R.E. Prehaustorial resistance to the wheat leaf rust fungus, Puccinia triticina, in Triticum monococcum (s.s.). Euphytica. 2001;117:3-12.</mixed-citation></citation-alternatives></ref><ref id="cit4"><label>4</label><citation-alternatives><mixed-citation xml:lang="ru">Babayeva S., Akparov Z., Abbasov M., Mammadov A., Zaifizadeh M., Street K. Diversity analysis of Central Asia and Caucasian lentil (Lens culinaris Medikus) germplasm using SSR fingerprinting. Genet. Res. Crop. Evol. 2009;56:293-298.</mixed-citation><mixed-citation xml:lang="en">Babayeva S., Akparov Z., Abbasov M., Mammadov A., Zaifizadeh M., Street K. Diversity analysis of Central Asia and Caucasian lentil (Lens culinaris Medikus) germplasm using SSR fingerprinting. Genet. Res. Crop. Evol. 2009;56:293-298.</mixed-citation></citation-alternatives></ref><ref id="cit5"><label>5</label><citation-alternatives><mixed-citation xml:lang="ru">Bahrai S., Jaradat A.A., Jaradat A.A. Diversity in seed storage proteins of T. boeoticum and T. urartu. Triticeae III. Proc. 3rd Int. Triticeae Symp. Aleppo, Syria, 4–8 May. 1998:237-243.</mixed-citation><mixed-citation xml:lang="en">Bahrai S., Jaradat A.A., Jaradat A.A. Diversity in seed storage proteins of T. boeoticum and T. urartu. Triticeae III. Proc. 3rd Int. Triticeae Symp. Aleppo, Syria, 4–8 May. 1998:237-243.</mixed-citation></citation-alternatives></ref><ref id="cit6"><label>6</label><citation-alternatives><mixed-citation xml:lang="ru">Bohn M., Utz H.F., Melchinger A.E. Genetic similarities among winter wheat cultivars determined on the basis of RFLPs, AFLPs and SSRs and their use for predicting progeny variance. Crop Sci. 1999;39: 228-237.</mixed-citation><mixed-citation xml:lang="en">Bohn M., Utz H.F., Melchinger A.E. Genetic similarities among winter wheat cultivars determined on the basis of RFLPs, AFLPs and SSRs and their use for predicting progeny variance. Crop Sci. 1999;39: 228-237.</mixed-citation></citation-alternatives></ref><ref id="cit7"><label>7</label><citation-alternatives><mixed-citation xml:lang="ru">Bossolini E., Krattinger S.G., Keller B. Development of simple sequence repeat markers specific for the Lr34 resistance region of wheat using sequence information from rice and Aegilops tauschii. Theor. Appl. Genet. 2006;113:1049-1062.</mixed-citation><mixed-citation xml:lang="en">Bossolini E., Krattinger S.G., Keller B. Development of simple sequence repeat markers specific for the Lr34 resistance region of wheat using sequence information from rice and Aegilops tauschii. Theor. Appl. Genet. 2006;113:1049-1062.</mixed-citation></citation-alternatives></ref><ref id="cit8"><label>8</label><citation-alternatives><mixed-citation xml:lang="ru">Burkhamer R.L., Lanning S.P., Martens R.J., Martin J.N., Talbert L.E. Predicting progeny variance from parental divergence in hard red spring wheat. Crop Sci. 1998;38:234-248.</mixed-citation><mixed-citation xml:lang="en">Burkhamer R.L., Lanning S.P., Martens R.J., Martin J.N., Talbert L.E. Predicting progeny variance from parental divergence in hard red spring wheat. Crop Sci. 1998;38:234-248.</mixed-citation></citation-alternatives></ref><ref id="cit9"><label>9</label><citation-alternatives><mixed-citation xml:lang="ru">Chen H.B., Martin J.M., Lavin M., Talbert L.E. Genetic diversity in hard red spring wheat based on sequence-tagged-site PCR markers. Crop Sci. 1994;34:1629-1632.</mixed-citation><mixed-citation xml:lang="en">Chen H.B., Martin J.M., Lavin M., Talbert L.E. Genetic diversity in hard red spring wheat based on sequence-tagged-site PCR markers. Crop Sci. 1994;34:1629-1632.</mixed-citation></citation-alternatives></ref><ref id="cit10"><label>10</label><citation-alternatives><mixed-citation xml:lang="ru">Cifci E.A., Yagdi K. Study of genetic diversity in wheat (Triticum aestivum) varieties using random amplified polymorphic DNA (RAPD) analysis. Turk. J. Field Crops. 2012;17:91-95.</mixed-citation><mixed-citation xml:lang="en">Cifci E.A., Yagdi K. Study of genetic diversity in wheat (Triticum aestivum) varieties using random amplified polymorphic DNA (RAPD) analysis. Turk. J. Field Crops. 2012;17:91-95.</mixed-citation></citation-alternatives></ref><ref id="cit11"><label>11</label><citation-alternatives><mixed-citation xml:lang="ru">Cox T.S., Lookhart G.L., Walker D.E., Harrell L.G., Albers L.D., Rodgers D.M. Genetic relationships among hard red winter wheat cultivars as evaluated by pedigree analysis and gliadin polyacrylamide-gel electrophoretic patterns. Crop Sci. 1985;25:1058-1063.</mixed-citation><mixed-citation xml:lang="en">Cox T.S., Lookhart G.L., Walker D.E., Harrell L.G., Albers L.D., Rodgers D.M. Genetic relationships among hard red winter wheat cultivars as evaluated by pedigree analysis and gliadin polyacrylamide-gel electrophoretic patterns. Crop Sci. 1985;25:1058-1063.</mixed-citation></citation-alternatives></ref><ref id="cit12"><label>12</label><citation-alternatives><mixed-citation xml:lang="ru">Dreisigacker S., Zhang P., Warburton M.L., Van Ginkel M., Hoisington D., Bohn M., Melchinger A.E. SSR and pedigree analyses of genetic diversity among CIMMYT wheat lines targeted to different megaenvironments. Crop Sci. 2004;44(2):381-388.</mixed-citation><mixed-citation xml:lang="en">Dreisigacker S., Zhang P., Warburton M.L., Van Ginkel M., Hoisington D., Bohn M., Melchinger A.E. SSR and pedigree analyses of genetic diversity among CIMMYT wheat lines targeted to different megaenvironments. Crop Sci. 2004;44(2):381-388.</mixed-citation></citation-alternatives></ref><ref id="cit13"><label>13</label><citation-alternatives><mixed-citation xml:lang="ru">Drikvand R., Bihamta M.R., Najafian G., Ebrahimi A. Investigation of genetic diversity among bread wheat cultivars (Triticum aestivum L.) using SSR markers. J. Agric. Sci. 2012;5(1):122.</mixed-citation><mixed-citation xml:lang="en">Drikvand R., Bihamta M.R., Najafian G., Ebrahimi A. Investigation of genetic diversity among bread wheat cultivars (Triticum aestivum L.) using SSR markers. J. Agric. Sci. 2012;5(1):122.</mixed-citation></citation-alternatives></ref><ref id="cit14"><label>14</label><citation-alternatives><mixed-citation xml:lang="ru">Dvorak J., Terlizzi P.D., Zhang H.B., Resta P. The evolution of polyploid wheats: identification of the A genome donor species. Genome. 1993;36:21-31.</mixed-citation><mixed-citation xml:lang="en">Dvorak J., Terlizzi P.D., Zhang H.B., Resta P. The evolution of polyploid wheats: identification of the A genome donor species. Genome. 1993;36:21-31.</mixed-citation></citation-alternatives></ref><ref id="cit15"><label>15</label><citation-alternatives><mixed-citation xml:lang="ru">Farouji A., Khodayari H., Saeidi H., Rahiminejad M.R. Genetic diversity of diploid Triticum species in Iran assessed using inter-retroelement amplified polymorphisms (IRAP) markers. Biologia. 2015; 1:52-60.</mixed-citation><mixed-citation xml:lang="en">Farouji A., Khodayari H., Saeidi H., Rahiminejad M.R. Genetic diversity of diploid Triticum species in Iran assessed using inter-retroelement amplified polymorphisms (IRAP) markers. Biologia. 2015; 1:52-60.</mixed-citation></citation-alternatives></ref><ref id="cit16"><label>16</label><citation-alternatives><mixed-citation xml:lang="ru">Figliuolo G., Perrino P. Genetic diversity and intra-specific phylogeny of Triticum turgidum L. subsp. dicoccon (Schrank) Thell. revealed by RFLPs and SSRs. Genet. Resour. Crop Evol. 2004;51:519-527.</mixed-citation><mixed-citation xml:lang="en">Figliuolo G., Perrino P. Genetic diversity and intra-specific phylogeny of Triticum turgidum L. subsp. dicoccon (Schrank) Thell. revealed by RFLPs and SSRs. Genet. Resour. Crop Evol. 2004;51:519-527.</mixed-citation></citation-alternatives></ref><ref id="cit17"><label>17</label><citation-alternatives><mixed-citation xml:lang="ru">Gascuel O. Concerning the NJ algorithm and its unweighted version, UNJ. Mathematical Hierarchies and Biology. DIMACS Series in Discrete Mathematics and Theoretical Computer Science. Provi-dence: Am. Math. Soc. 1997;37:149-170.</mixed-citation><mixed-citation xml:lang="en">Gascuel O. Concerning the NJ algorithm and its unweighted version, UNJ. Mathematical Hierarchies and Biology. DIMACS Series in Discrete Mathematics and Theoretical Computer Science. Provi-dence: Am. Math. Soc. 1997;37:149-170.</mixed-citation></citation-alternatives></ref><ref id="cit18"><label>18</label><citation-alternatives><mixed-citation xml:lang="ru">Ghaderi A., Adams M.W., Nassib A.M. Relationship between genetic distance and heterosis for yield and morphological traits in dry edible bean and faba bean. Crop Sci. 1984;24:37-24.</mixed-citation><mixed-citation xml:lang="en">Ghaderi A., Adams M.W., Nassib A.M. Relationship between genetic distance and heterosis for yield and morphological traits in dry edible bean and faba bean. Crop Sci. 1984;24:37-24.</mixed-citation></citation-alternatives></ref><ref id="cit19"><label>19</label><citation-alternatives><mixed-citation xml:lang="ru">Hajiyev E.S., Akparov Z.I., Aliyev R.T., Saidova S.V., Izzatullayeva V.I., Babayeva S.M., Abbasov M.A. Genetic polymorphism of durum wheat (Triticum durum Desf.) accessions of Azerbaijan. Russ. J. Genet. 2015;51:863-870.</mixed-citation><mixed-citation xml:lang="en">Hajiyev E.S., Akparov Z.I., Aliyev R.T., Saidova S.V., Izzatullayeva V.I., Babayeva S.M., Abbasov M.A. Genetic polymorphism of durum wheat (Triticum durum Desf.) accessions of Azerbaijan. Russ. J. Genet. 2015;51:863-870.</mixed-citation></citation-alternatives></ref><ref id="cit20"><label>20</label><citation-alternatives><mixed-citation xml:lang="ru">Hammer K., Filatenko A.A., Korzun V. Microsatellite markers – a new tool for distinguishing diploid wheat species. Genet. Resour. Crop Evol. 2000;47(5):497-505.</mixed-citation><mixed-citation xml:lang="en">Hammer K., Filatenko A.A., Korzun V. Microsatellite markers – a new tool for distinguishing diploid wheat species. Genet. Resour. Crop Evol. 2000;47(5):497-505.</mixed-citation></citation-alternatives></ref><ref id="cit21"><label>21</label><citation-alternatives><mixed-citation xml:lang="ru">Harjit-Singh X., Dhaliwal H.S., Yifru-Teklu Y., Singh H. Germplasm enhancement through wide hybridization and molecular breeding. 11th Regional Wheat Workshop Eastern Central and Southern Af¬rica, Addis-Abeba, Ethiopia, September. 2000;18-22.</mixed-citation><mixed-citation xml:lang="en">Harjit-Singh X., Dhaliwal H.S., Yifru-Teklu Y., Singh H. Germplasm enhancement through wide hybridization and molecular breeding. 11th Regional Wheat Workshop Eastern Central and Southern Africa, Addis-Abeba, Ethiopia, September. 2000;18-22.</mixed-citation></citation-alternatives></ref><ref id="cit22"><label>22</label><citation-alternatives><mixed-citation xml:lang="ru">Heun M., Schafer-Pregl R., Klawan D., Castagna R., Accerbi M., Borghi B., Salamini F. Site of Einkorn wheat domestication identi¬fied by DNA fingerprinting. Science. 1997;278:1312-1314.</mixed-citation><mixed-citation xml:lang="en">Heun M., Schafer-Pregl R., Klawan D., Castagna R., Accerbi M., Borghi B., Salamini F. Site of Einkorn wheat domestication identified by DNA fingerprinting. Science. 1997;278:1312-1314.</mixed-citation></citation-alternatives></ref><ref id="cit23"><label>23</label><citation-alternatives><mixed-citation xml:lang="ru">Izzatullayeva V.İ., Akparov Z.I., Babayeva S.M., Ojaghi J., Abbasov M.A. Efficiency of using RAPD and ISSR markers in evaluation of genetic diversity in sugar beet. Turk. J. Biol. 2014;38:429-438.</mixed-citation><mixed-citation xml:lang="en">Izzatullayeva V.İ., Akparov Z.I., Babayeva S.M., Ojaghi J., Abbasov M.A. Efficiency of using RAPD and ISSR markers in evaluation of genetic diversity in sugar beet. Turk. J. Biol. 2014;38:429-438.</mixed-citation></citation-alternatives></ref><ref id="cit24"><label>24</label><citation-alternatives><mixed-citation xml:lang="ru">Kojima T., Nagaoka T., Noda K., Ogihara Y. Genetic linkage map of ISSR and RAPD markers in Einkorn wheat in relation to that of RFLP markers. Theor. Appl. Genet. 1998;96:37-45.</mixed-citation><mixed-citation xml:lang="en">Kojima T., Nagaoka T., Noda K., Ogihara Y. Genetic linkage map of ISSR and RAPD markers in Einkorn wheat in relation to that of RFLP markers. Theor. Appl. Genet. 1998;96:37-45.</mixed-citation></citation-alternatives></ref><ref id="cit25"><label>25</label><citation-alternatives><mixed-citation xml:lang="ru">Konarev V.G., Gavrilyuk I.P., Peneva T.I., Konarev A.V., Khakimova A.G., Migushova E.F. On the nature and origin of wheat genomes with regard to the data on the biochemistry and immunochemistry of grain proteins. Selskokhozyaystvennaya Biologiya = Agricultural Biology. 1976;11(5):656-665. (in Russian)</mixed-citation><mixed-citation xml:lang="en">Konarev V.G., Gavrilyuk I.P., Peneva T.I., Konarev A.V., Khakimova A.G., Migushova E.F. On the nature and origin of wheat genomes with regard to the data on the biochemistry and immunochemistry of grain proteins. Selskokhozyaystvennaya Biologiya = Agricultural Biology. 1976;11(5):656-665. (in Russian)</mixed-citation></citation-alternatives></ref><ref id="cit26"><label>26</label><citation-alternatives><mixed-citation xml:lang="ru">Korzun V., Röder M., Ganal M., Filatenko A., Hammer K. Genetic diversity and evolution of the diploid wheat T. urartu, T. boeoticum and T. monococcum revealed by microsatellite markers. Schr. Genet. Ressour. 1998;8:244-247.</mixed-citation><mixed-citation xml:lang="en">Korzun V., Röder M., Ganal M., Filatenko A., Hammer K. Genetic diversity and evolution of the diploid wheat T. urartu, T. boeoticum and T. monococcum revealed by microsatellite markers. Schr. Genet. Ressour. 1998;8:244-247.</mixed-citation></citation-alternatives></ref><ref id="cit27"><label>27</label><citation-alternatives><mixed-citation xml:lang="ru">Kumar S., Kumar V., Kumari P., Singh A.K., Singh R. DNA fingerprinting and genetic diversity studies in wheat genotypes using SSR markers. J. Environ. Biol. 2016;37(2):319.</mixed-citation><mixed-citation xml:lang="en">Kumar S., Kumar V., Kumari P., Singh A.K., Singh R. DNA fingerprinting and genetic diversity studies in wheat genotypes using SSR markers. J. Environ. Biol. 2016;37(2):319.</mixed-citation></citation-alternatives></ref><ref id="cit28"><label>28</label><citation-alternatives><mixed-citation xml:lang="ru">Liu K., Muse S.V. PowerMarker: Integrated analysis enviornment for genetic marker data. Bioinformatic. 2005;21:2128-2129.</mixed-citation><mixed-citation xml:lang="en">Liu K., Muse S.V. PowerMarker: Integrated analysis enviornment for genetic marker data. Bioinformatic. 2005;21:2128-2129.</mixed-citation></citation-alternatives></ref><ref id="cit29"><label>29</label><citation-alternatives><mixed-citation xml:lang="ru">Malaki M., Naghavi M.R., Alizadeh H., Potki P., Kazemi M., Pirseyedi S.M., Mardi M., Tabatabaei F. Study of genetic variation in wild diploid wheat (Triticum boeoticum) from Iran using AFLP markers. Iran. J. Biotech. 2006;4:269-274.</mixed-citation><mixed-citation xml:lang="en">Malaki M., Naghavi M.R., Alizadeh H., Potki P., Kazemi M., Pirseyedi S.M., Mardi M., Tabatabaei F. Study of genetic variation in wild diploid wheat (Triticum boeoticum) from Iran using AFLP markers. Iran. J. Biotech. 2006;4:269-274.</mixed-citation></citation-alternatives></ref><ref id="cit30"><label>30</label><citation-alternatives><mixed-citation xml:lang="ru">Malik R., Tiwari R., Arora A., Kumar P., Sheoran S., Sharma P., Singh R., Tiwari V., Sharma I. Genotypic characterization of elite Indian wheat genotypes using molecular markers and their pedigree analysis. Aust. J. Crop Sci. 2013;7:561-567.</mixed-citation><mixed-citation xml:lang="en">Malik R., Tiwari R., Arora A., Kumar P., Sheoran S., Sharma P., Singh R., Tiwari V., Sharma I. Genotypic characterization of elite Indian wheat genotypes using molecular markers and their pedigree analysis. Aust. J. Crop Sci. 2013;7:561-567.</mixed-citation></citation-alternatives></ref><ref id="cit31"><label>31</label><citation-alternatives><mixed-citation xml:lang="ru">McLauchlan A., Henry R.J., Issac P.G., Edwards K.J. Microsatellite analysis in cultivated hexaploid wheat and wild wheat rela¬tives. Ed. R.J. Henry. Plant Genotyping: The DNA Fingerprinting of Plants. Wallingford, UK: CABI Publishing, CAB International, 2001;147-159.</mixed-citation><mixed-citation xml:lang="en">McLauchlan A., Henry R.J., Issac P.G., Edwards K.J. Microsatellite analysis in cultivated hexaploid wheat and wild wheat rela¬tives. Ed. R.J. Henry. Plant Genotyping: The DNA Fingerprinting of Plants. Wallingford, UK: CABI Publishing, CAB International, 2001;147-159.</mixed-citation></citation-alternatives></ref><ref id="cit32"><label>32</label><citation-alternatives><mixed-citation xml:lang="ru">Medini M., Hamza S., Rebai A., Baum M. Analysis of genetic diversity in Tunisian durum wheat cultivars and related wild species by SSR and AFLP markers. Genet. Resour. Crop Evol. 2005;52:21-31.</mixed-citation><mixed-citation xml:lang="en">Medini M., Hamza S., Rebai A., Baum M. Analysis of genetic diversity in Tunisian durum wheat cultivars and related wild species by SSR and AFLP markers. Genet. Resour. Crop Evol. 2005;52:21-31.</mixed-citation></citation-alternatives></ref><ref id="cit33"><label>33</label><citation-alternatives><mixed-citation xml:lang="ru">Mizumoto K., Hirosawa S., Nakamura C., Takumi S. Nuclear and chloroplast genome genetic diversity in the wild einkorn wheat, Triticum urartu, revealed by AFLP and SSLP analyses. Hereditas. 2002;137: 208-214.</mixed-citation><mixed-citation xml:lang="en">Mizumoto K., Hirosawa S., Nakamura C., Takumi S. Nuclear and chloroplast genome genetic diversity in the wild einkorn wheat, Triticum urartu, revealed by AFLP and SSLP analyses. Hereditas. 2002;137: 208-214.</mixed-citation></citation-alternatives></ref><ref id="cit34"><label>34</label><citation-alternatives><mixed-citation xml:lang="ru">Moghaddam M., Ehdaie B., Waines J.G. Genetic diversity in populations of wild diploid wheat Triticum urartu Tum. ex. Gandil. revealed by isozyme markers. Genet. Res. Crop Evol. 2000;47(3):323-334.</mixed-citation><mixed-citation xml:lang="en">Moghaddam M., Ehdaie B., Waines J.G. Genetic diversity in populations of wild diploid wheat Triticum urartu Tum. ex. Gandil. revealed by isozyme markers. Genet. Res. Crop Evol. 2000;47(3):323-334.</mixed-citation></citation-alternatives></ref><ref id="cit35"><label>35</label><citation-alternatives><mixed-citation xml:lang="ru">Motawei M.I., Al-Doss A.A., Moustafa K.A. Genetic diversity among selected wheat lines differing in heat tolerance using molecular markers. J. Food Agr. Environ. 2007;5:180-183.</mixed-citation><mixed-citation xml:lang="en">Motawei M.I., Al-Doss A.A., Moustafa K.A. Genetic diversity among selected wheat lines differing in heat tolerance using molecular markers. J. Food Agr. Environ. 2007;5:180-183.</mixed-citation></citation-alternatives></ref><ref id="cit36"><label>36</label><citation-alternatives><mixed-citation xml:lang="ru">Mousavifard S.S., Saeidi H., Rahiminejad M.R., Shamsadini M. Molecular analysis of diversity of diploid Triticum species in Iran us¬ing ISSR markers. Genet. Resour. Crop Evol. 2014. DOI 10.1007/s10722-014-0160-z.</mixed-citation><mixed-citation xml:lang="en">Mousavifard S.S., Saeidi H., Rahiminejad M.R., Shamsadini M. Molecular analysis of diversity of diploid Triticum species in Iran using ISSR markers. Genet. Resour. Crop Evol. 2014. DOI 10.1007/s10722-014-0160-z.</mixed-citation></citation-alternatives></ref><ref id="cit37"><label>37</label><citation-alternatives><mixed-citation xml:lang="ru">Naghavi M., Ebrahimi A., Sabokdast M., Mardi M. Assessment of genetic variation among five hordeum species from Iran. Cer. Res. Commun. 2011;39(4):487-496. http://www.jstor.org/stable/23792314</mixed-citation><mixed-citation xml:lang="en">Naghavi M., Ebrahimi A., Sabokdast M., Mardi M. Assessment of genetic variation among five hordeum species from Iran. Cer. Res. Commun. 2011;39(4):487-496. http://www.jstor.org/stable/23792314</mixed-citation></citation-alternatives></ref><ref id="cit38"><label>38</label><citation-alternatives><mixed-citation xml:lang="ru">Naghavi M.R., Malaki M., Alizadeh H., Pirseiedi M., Mardi M. An assessment of genetic diversity in wild diploid wheat Triticum boeoti¬cum from west of Iran using RAPD, AFLP and SSR markers. J. Agr. Sci. Tech. 2009;11:585-598.</mixed-citation><mixed-citation xml:lang="en">Naghavi M.R., Malaki M., Alizadeh H., Pirseiedi M., Mardi M. An assessment of genetic diversity in wild diploid wheat Triticum boeoti¬cum from west of Iran using RAPD, AFLP and SSR markers. J. Agr. Sci. Tech. 2009;11:585-598.</mixed-citation></citation-alternatives></ref><ref id="cit39"><label>39</label><citation-alternatives><mixed-citation xml:lang="ru">Naghavi M.R., Mardi M., Ramshini H.A., Fazelinasab B. Comparative analyses of the genetic diversity among bread wheat genotypes based on RAPD and SSR markers. Iran. J. Biotechnol. 2004;2:195-202.</mixed-citation><mixed-citation xml:lang="en">Naghavi M.R., Mardi M., Ramshini H.A., Fazelinasab B. Comparative analyses of the genetic diversity among bread wheat genotypes based on RAPD and SSR markers. Iran. J. Biotechnol. 2004;2:195-202.</mixed-citation></citation-alternatives></ref><ref id="cit40"><label>40</label><citation-alternatives><mixed-citation xml:lang="ru">Ovesná J., Kučera L., Bocková R., Holubec V. Characterisation of powdery mildew resistance donors within Triticum boeoticum accessions using RAPDs. Czech. J. Genet. Plant Breed. 2002;38:117-124.</mixed-citation><mixed-citation xml:lang="en">Ovesná J., Kučera L., Bocková R., Holubec V. Characterisation of powdery mildew resistance donors within Triticum boeoticum accessions using RAPDs. Czech. J. Genet. Plant Breed. 2002;38:117-124.</mixed-citation></citation-alternatives></ref><ref id="cit41"><label>41</label><citation-alternatives><mixed-citation xml:lang="ru">Perrier X., Jacquemoud-Collet J.P. DARwin software http://darwin.cirad.fr/darwin.2006</mixed-citation><mixed-citation xml:lang="en">Perrier X., Jacquemoud-Collet J.P. DARwin software http://darwin.cirad.fr/darwin.2006</mixed-citation></citation-alternatives></ref><ref id="cit42"><label>42</label><citation-alternatives><mixed-citation xml:lang="ru">Pestsova E., Ganal M.W., Roder M.S. Isolation and mapping of microsatellite markers specific for the D genome of bread wheat. Genome. 2000;43:689-697.</mixed-citation><mixed-citation xml:lang="en">Pestsova E., Ganal M.W., Roder M.S. Isolation and mapping of microsatellite markers specific for the D genome of bread wheat. Genome. 2000;43:689-697.</mixed-citation></citation-alternatives></ref><ref id="cit43"><label>43</label><citation-alternatives><mixed-citation xml:lang="ru">Prasad M., Varshney R.K., Roy J.K., Balyan H.S., Gupta P.K. The use of microsatellites for detecting DNA polymorphism, genotype identification and genetic diversity in wheat. Theor. Appl. Genet. 2000; 100:584-592.</mixed-citation><mixed-citation xml:lang="en">Prasad M., Varshney R.K., Roy J.K., Balyan H.S., Gupta P.K. The use of microsatellites for detecting DNA polymorphism, genotype identification and genetic diversity in wheat. Theor. Appl. Genet. 2000; 100:584-592.</mixed-citation></citation-alternatives></ref><ref id="cit44"><label>44</label><citation-alternatives><mixed-citation xml:lang="ru">Röder M.S., Korzun V., Wendehake K., Plaschke J., Tixier M.H., Leroy P., Ganal M.W. A microsatellite map of wheat. Genetics. 1998; 149:2007-2023.</mixed-citation><mixed-citation xml:lang="en">Röder M.S., Korzun V., Wendehake K., Plaschke J., Tixier M.H., Leroy P., Ganal M.W. A microsatellite map of wheat. Genetics. 1998; 149:2007-2023.</mixed-citation></citation-alternatives></ref><ref id="cit45"><label>45</label><citation-alternatives><mixed-citation xml:lang="ru">Song Q.J., Fickus E.W., Cregan P.B. Characterization of trinucleotide SSR motifs in wheat. Theor. Appl. Genet. 2002;104:286-293.</mixed-citation><mixed-citation xml:lang="en">Song Q.J., Fickus E.W., Cregan P.B. Characterization of trinucleotide SSR motifs in wheat. Theor. Appl. Genet. 2002;104:286-293.</mixed-citation></citation-alternatives></ref><ref id="cit46"><label>46</label><citation-alternatives><mixed-citation xml:lang="ru">Song Q.J., Shi J.R., Singh S., Fickus E.W., Costa J.M., Lewis J., Gill B.S., Ward R., Cregan P.B. Development and mapping of microsatellite (SSR) markers in wheat. Theor. Appl. Genet. 2005;110: 550-560.</mixed-citation><mixed-citation xml:lang="en">Song Q.J., Shi J.R., Singh S., Fickus E.W., Costa J.M., Lewis J., Gill B.S., Ward R., Cregan P.B. Development and mapping of microsatellite (SSR) markers in wheat. Theor. Appl. Genet. 2005;110: 550-560.</mixed-citation></citation-alternatives></ref><ref id="cit47"><label>47</label><citation-alternatives><mixed-citation xml:lang="ru">Španić V., Buerstmayr H., Drezner G. Assessment of genetic diversity of wheat genotypes using microsatellite markers. Period. Biol. 2012; 114(1):37-42.</mixed-citation><mixed-citation xml:lang="en">Song Q.J., Shi J.R., Singh S., Fickus E.W., Costa J.M., Lewis J., Gill B.S., Ward R., Cregan P.B. Development and mapping of microsatellite (SSR) markers in wheat. Theor. Appl. Genet. 2005;110: 550-560.</mixed-citation></citation-alternatives></ref><ref id="cit48"><label>48</label><citation-alternatives><mixed-citation xml:lang="ru">Sud S., Bains N.S., Nanda G.S. Genetic relationships among wheat genotypes, as revealed by microsatellite markers and pedigree analysis. J. Appl. Genet. 2005;46:375-379.</mixed-citation><mixed-citation xml:lang="en">Španić V., Buerstmayr H., Drezner G. Assessment of genetic diversity of wheat genotypes using microsatellite markers. Period. Biol. 2012; 114(1):37-42.</mixed-citation></citation-alternatives></ref><ref id="cit49"><label>49</label><citation-alternatives><mixed-citation xml:lang="ru">Takumi S., Nasuda S., Liu Y.G., Tsunewaki K. Wheat phylogeny determined by RFLP analysis of nuclear DNA. Einkorn Wheat. Jpn. J. Genet. 1993;68:73-79.</mixed-citation><mixed-citation xml:lang="en">Španić V., Buerstmayr H., Drezner G. Assessment of genetic diversity of wheat genotypes using microsatellite markers. Period. Biol. 2012; 114(1):37-42.</mixed-citation></citation-alternatives></ref><ref id="cit50"><label>50</label><citation-alternatives><mixed-citation xml:lang="ru">Wang X., Luo G., Yang W., Li Y., Sun J., Zhan K., Liu D., Zhang A. Genetic diversity, population structure and marker-trait associations for agronomic and grain traits in wild diploid wheat Triticum urartu. BMC Plant. Biol. 2017;17:112.</mixed-citation><mixed-citation xml:lang="en">Sud S., Bains N.S., Nanda G.S. Genetic relationships among wheat genotypes, as revealed by microsatellite markers and pedigree analysis. J. Appl. Genet. 2005;46:375-379.</mixed-citation></citation-alternatives></ref><ref id="cit51"><label>51</label><citation-alternatives><mixed-citation xml:lang="ru">Zeb B., Khan I.A., Ali S., Bacha S., Mumtaz S., Swati Z.A. Study on genetic diversity in Pakistani wheat varieties using simple sequence repeat (SSR) markers. Afr. J. Biotechnol. 2009;8:4016-4019.</mixed-citation><mixed-citation xml:lang="en">Sud S., Bains N.S., Nanda G.S. Genetic relationships among wheat genotypes, as revealed by microsatellite markers and pedigree analysis. J. Appl. Genet. 2005;46:375-379.</mixed-citation></citation-alternatives></ref><ref id="cit52"><label>52</label><citation-alternatives><mixed-citation xml:lang="ru">Zeshan A., Afzal M., Alghamdi S., Kettener K., Mubashar A., Mubushar M., Shakeel A. Evaluation of genetic diversity amongthe Paki¬stani wheat (Triticum aestivum L.) lines through random molecular markers. Braz. Arch. Biol. Technol. 2016;59:e16160282.</mixed-citation><mixed-citation xml:lang="en">Takumi S., Nasuda S., Liu Y.G., Tsunewaki K. Wheat phylogeny determined by RFLP analysis of nuclear DNA. Einkorn Wheat. Jpn. J. Genet. 1993;68:73-79.</mixed-citation></citation-alternatives></ref><ref id="cit53"><label>53</label><citation-alternatives><mixed-citation xml:lang="ru">Zhang D., Bai G., Zhu C., Yu J., Carver B.F. Genetic diversity, population structure, and linkage disequilibrium in U.S. elite winter wheat. Plant Genome. 2010;3:117-127.</mixed-citation><mixed-citation xml:lang="en">Takumi S., Nasuda S., Liu Y.G., Tsunewaki K. Wheat phylogeny determined by RFLP analysis of nuclear DNA. Einkorn Wheat. Jpn. J. Genet. 1993;68:73-79.</mixed-citation></citation-alternatives></ref><ref id="cit54"><label>54</label><citation-alternatives><mixed-citation xml:lang="ru">Zohary D., Hopf M. Domestication of plants in the Old World: the Origin and Spread of Cultivated Plants in West Asia, Europe, and the Nile Valley. 2nd ed. Oxford: New York: Clarendon Press, 1993.</mixed-citation><mixed-citation xml:lang="en">Wang X., Luo G., Yang W., Li Y., Sun J., Zhan K., Liu D., Zhang A. Genetic diversity, population structure and marker-trait associations for agronomic and grain traits in wild diploid wheat Triticum urartu. BMC Plant. Biol. 2017;17:112.</mixed-citation></citation-alternatives></ref><ref id="cit55"><label>55</label><citation-alternatives><mixed-citation xml:lang="ru">Wang X., Luo G., Yang W., Li Y., Sun J., Zhan K., Liu D., Zhang A. Genetic diversity, population structure and marker-trait associations for agronomic and grain traits in wild diploid wheat Triticum urartu. BMC Plant. Biol. 2017;17:112.</mixed-citation><mixed-citation xml:lang="en">Wang X., Luo G., Yang W., Li Y., Sun J., Zhan K., Liu D., Zhang A. Genetic diversity, population structure and marker-trait associations for agronomic and grain traits in wild diploid wheat Triticum urartu. BMC Plant. Biol. 2017;17:112.</mixed-citation></citation-alternatives></ref><ref id="cit56"><label>56</label><citation-alternatives><mixed-citation xml:lang="ru">Zeb B., Khan I.A., Ali S., Bacha S., Mumtaz S., Swati Z.A. Study on genetic diversity in Pakistani wheat varieties using simple sequence repeat (SSR) markers. Afr. J. Biotechnol. 2009;8:4016-4019.</mixed-citation><mixed-citation xml:lang="en">Zeb B., Khan I.A., Ali S., Bacha S., Mumtaz S., Swati Z.A. Study on genetic diversity in Pakistani wheat varieties using simple sequence repeat (SSR) markers. Afr. J. Biotechnol. 2009;8:4016-4019.</mixed-citation></citation-alternatives></ref><ref id="cit57"><label>57</label><citation-alternatives><mixed-citation xml:lang="ru">Zeb B., Khan I.A., Ali S., Bacha S., Mumtaz S., Swati Z.A. Study on genetic diversity in Pakistani wheat varieties using simple sequence repeat (SSR) markers. Afr. J. Biotechnol. 2009;8:4016-4019.</mixed-citation><mixed-citation xml:lang="en">Zeb B., Khan I.A., Ali S., Bacha S., Mumtaz S., Swati Z.A. Study on genetic diversity in Pakistani wheat varieties using simple sequence repeat (SSR) markers. Afr. J. Biotechnol. 2009;8:4016-4019.</mixed-citation></citation-alternatives></ref><ref id="cit58"><label>58</label><citation-alternatives><mixed-citation xml:lang="ru">Zeshan A., Afzal M., Alghamdi S., Kettener K., Mubashar A., Mubushar M., Shakeel A. Evaluation of genetic diversity amongthe Pakistani wheat (Triticum aestivum L.) lines through random molecular markers. Braz. Arch. Biol. Technol. 2016;59:e16160282.</mixed-citation><mixed-citation xml:lang="en">Zeshan A., Afzal M., Alghamdi S., Kettener K., Mubashar A., Mubushar M., Shakeel A. Evaluation of genetic diversity amongthe Pakistani wheat (Triticum aestivum L.) lines through random molecular markers. Braz. Arch. Biol. Technol. 2016;59:e16160282.</mixed-citation></citation-alternatives></ref><ref id="cit59"><label>59</label><citation-alternatives><mixed-citation xml:lang="ru">Zeshan A., Afzal M., Alghamdi S., Kettener K., Mubashar A., Mubushar M., Shakeel A. Evaluation of genetic diversity amongthe Pakistani wheat (Triticum aestivum L.) lines through random molecular markers. Braz. Arch. Biol. Technol. 2016;59:e16160282.</mixed-citation><mixed-citation xml:lang="en">Zeshan A., Afzal M., Alghamdi S., Kettener K., Mubashar A., Mubushar M., Shakeel A. Evaluation of genetic diversity amongthe Pakistani wheat (Triticum aestivum L.) lines through random molecular markers. Braz. Arch. Biol. Technol. 2016;59:e16160282.</mixed-citation></citation-alternatives></ref><ref id="cit60"><label>60</label><citation-alternatives><mixed-citation xml:lang="ru">Zhang D., Bai G., Zhu C., Yu J., Carver B.F. Genetic diversity, population structure, and linkage disequilibrium in U.S. elite winter wheat. Plant Genome. 2010;3:117-127.</mixed-citation><mixed-citation xml:lang="en">Zhang D., Bai G., Zhu C., Yu J., Carver B.F. Genetic diversity, population structure, and linkage disequilibrium in U.S. elite winter wheat. Plant Genome. 2010;3:117-127.</mixed-citation></citation-alternatives></ref><ref id="cit61"><label>61</label><citation-alternatives><mixed-citation xml:lang="ru">Zhang D., Bai G., Zhu C., Yu J., Carver B.F. Genetic diversity, population structure, and linkage disequilibrium in U.S. elite winter wheat. Plant Genome. 2010;3:117-127.</mixed-citation><mixed-citation xml:lang="en">Zhang D., Bai G., Zhu C., Yu J., Carver B.F. Genetic diversity, population structure, and linkage disequilibrium in U.S. elite winter wheat. Plant Genome. 2010;3:117-127.</mixed-citation></citation-alternatives></ref><ref id="cit62"><label>62</label><citation-alternatives><mixed-citation xml:lang="ru">Zohary D., Hopf M. Domestication of plants in the Old World: the Origin and Spread of Cultivated Plants in West Asia, Europe, and the Nile Valley. 2nd ed. Oxford: New York: Clarendon Press, 1993.</mixed-citation><mixed-citation xml:lang="en">Zohary D., Hopf M. Domestication of plants in the Old World: the Origin and Spread of Cultivated Plants in West Asia, Europe, and the Nile Valley. 2nd ed. Oxford: New York: Clarendon Press, 1993.</mixed-citation></citation-alternatives></ref><ref id="cit63"><label>63</label><citation-alternatives><mixed-citation xml:lang="ru">Zohary D., Hopf M. Domestication of plants in the Old World: the Origin and Spread of Cultivated Plants in West Asia, Europe, and the Nile Valley. 2nd ed. Oxford: New York: Clarendon Press, 1993.</mixed-citation><mixed-citation xml:lang="en">Zohary D., Hopf M. Domestication of plants in the Old World: the Origin and Spread of Cultivated Plants in West Asia, Europe, and the Nile Valley. 2nd ed. Oxford: New York: Clarendon Press, 1993.</mixed-citation></citation-alternatives></ref></ref-list><fn-group><fn fn-type="conflict"><p>The authors declare that there are no conflicts of interest present.</p></fn></fn-group></back></article>
