References

vavilov

Вавиловский журнал генетики и селекции

Vavilov Journal of Genetics and Breeding

2500-3259

Institute of Cytology and Genetics of Siberian Branch of the RAS

10.18699/VJ18.397

vavilov-1589

Research Article

ГЕНЕТИКА ЖИВОТНЫХ

ANIMAL GENETICS

Инвазивные виды Aedes albopictus и Aedes aegypti на Черноморском побережье Краснодарского края: генетика (COI, ITS2), зараженность Wolbachia и Dirofilaria

Invasive mosquito species Aedes albopictus and Aedes aegypti on the Black Sea coast of the Caucasus: genetics (COI, ITS2), Wolbachia and Dirofilaria infections

Шайкевич

Е. В.

Shaikevich

E. V.

elenashaikevich@mail.ru

Патраман

И. В.

Patraman

I. V.

Богачева

А. С.

Bogacheva

A. S.

Ракова

В. М.

Rakova

V. М.

Зеля

O. П.

Zelya

O. Р.

Ганушкина

Л. А.

Ganushkina

L. A.

Институт общей генетики им. Н.И. Вавилова РАНРоссияVavilov Institute of General Genetics, RASRussian Federation

Институт медицинской паразитологии, тропических и трансмиссивных заболеваний им. Е.И. Марциновского Первого Московского государственного медицинского университета им. И.М. Сеченова (Сеченовский университет) Министерства здравоохранения РФРоссияInstitute of Medical Parasitology and Tropical Medicine named after E. Martsinovskii, Sechenov First Moscow State Меdical UniversityRussian Federation

Московский государственный университет им. М.В. ЛомоносоваРоссияMoscow State UniversityRussian Federation

2018

08082018

225574585

2018

Шайкевич Е.В., Патраман И.В., Богачева А.С., Ракова В.М., Зеля O.П., Ганушкина Л.А.

Shaikevich E.V., Patraman I.V., Bogacheva A.S., Rakova V.М., Zelya O.Р., Ganushkina L.A.

This work is licensed under a Creative Commons Attribution 4.0 License.

https://vavilov.elpub.ru/jour/article/view/1589

Ареал инвазивных видов Aedes aegypti и Aedes albopictus, переносчиков ряда трансмиссивных инфекций, расширяется. Идентификация видов-переносчиков и понимание генетического разнообразия инвазивных популяций позволяют разработать соответствующие профилактические мероприятия. Эндосимбиотическая бактерия Wolbachia pipientis оказывает различные эффекты на своих хозяев-артропод и может влиять на процесс передачи и распространения возбудителей. Основной целью работы была молекулярно-генетическая идентификация видов комаров рода Aedes, собранных в населенных пунктах Черноморского побережья с 2007 по 2017 г.; определение генетической изменчивости Ae. aegypti, Ae. albopictus и их симбиотической бактерии Wolbachia; оценка способности Ae. aegypti и Ae. albopictus к заражению и распространению паразитических Dirof ilaria. Отдельной задачей являлась генетическая характеристика лабораторной линии Ae. aegypti ИМПиТМ, которая поддерживается в лаборатории в течение 50 лет. Исследованы маркеры ядерной и митохондриальной ДНК у Ae. albopictus и Ae. aegypti и проведено их сравнение с Ae. cretinus и Ae. koreiсus, симпатрически обитающими на данной территории, а также с Ae. aegypti лабораторной линии. Обнаружен низкий уровень изменчивости Ae. albopictus и Ae. aegypti, собранных в природе в разных точках сбора и в разное время. У Ae. albopictus выявлены четыре гаплотипа на основе сравнения вариабельной области внутреннего транскрибируемого спейсера (ITS2) кластера генов рРНК и два митохондриальных гаплотипа при сравнении последовательностей гена первой субъединицы цитохромоксидазы (COI). У Ae. aegypti, собранных в природе, обнаружены четыре гаплотипа ядерной ДНК и три митохондриальных гаплотипа. Более половины Ae. albopictus заражены Wolbachia. В популяции на Черноморском побережье Краснодарского края встречаются два штамма бактерии: wAlbA и wAlbB. Общая зараженность комаров Ae. aegypti и Ae. albopictus дирофиляриями составила 1.8 %. Dirof ilaria immitis обнаружены только в брюшках комаров, развития личинок до инфекционной стадии L3 не выявлено. Личинки D. repens развились до инфекционной стадии в комарах обоих видов.

The area of invasive species Aedes albopictus and Aedes aegypti is expanding. Precise identification and understanding of the genetic diversity of invasive mosquito populations allows us to develop appropriate control methods. Endosymbiotic bacterium Wolbachia pipientis has different effects on their arthropod hosts and can influence the transmission and spread of the pathogens. The objective of the presented study was molecular-genetic identification of the Aedes mosquitoes collected in sampling sites on the Black Sea coast from 2007 to 2017; determination of genetic variability of Ae. aegypti, Ae. albopictus and their symbiotic bacteria Wolbachia; assessment of mosquitoes ability to be infected and to spread parasitic Dirofilaria. Another objective was obtaining the genetic characteristic of laboratory strain Ae. aegypti IMPITM. We investigated two markers of nuclear and mitochondrial DNA from Ae. albopictus and Ae. aegypti and compared them to DNA from Ae. cretinus and Ae. koreiсus sympatrically inhabiting the territory, as well as to one of Ae. aegypti from a laboratory line. The study of nuclear and mitochondrial DNA revealed a low level of variability in the invasive mosquitoes Ae. albopictus and Ae. aegypti collected at different collection sites and in different years. More than a half of Ae. albopictus were infected with Wolbachia, two strains of bacteria, wAlbA and wAlbB, occur in the Ae. albopictus population on the Black Sea coast. Total infection of Ae. aegypti and Ae. albopictus with dirofilariae was 1.8 %. Dirofilaria immitis was found only in mosquito abdomen, larvae of infective stage L3 were not found. D. repens larvae developed to the infective stage in the mosquitoes of both species.

кровососущие комарыAedes aegyptiAedes albopictusинвазияпопуляцияЧерноморское побережье КавказаITS2COIWolbachiaDirofilaria

blood-sucking mosquitoesAedes aegyptiAedes albopictusinvasionpopulationBlack Sea coastITS2COIWolbachiaDirofilaria

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The authors declare that there are no conflicts of interest present.