References

vavilov

Вавиловский журнал генетики и селекции

Vavilov Journal of Genetics and Breeding

2500-3259

Institute of Cytology and Genetics of Siberian Branch of the RAS

10.18699/VJ18.392

vavilov-1593

Research Article

ГЕНОФОНД И СЕЛЕКЦИЯ РАСТЕНИЙ

PLANT GENE POOL AND BREEDING

Полногеномный анализ ассоциаций с устойчивостью к грибным болезням в коллекции сои в условиях Юго-Восточного и Южного Казахстана

GWAS of a soybean breeding collection from South East and South Kazakhstan for resistance to fungal diseases

Затыбеков

А.

Zatybekov

Абугалиева

С.

Abugalieva

Дидоренко

С.

Didorenko

Рсалиев

А.

Rsaliyev

Туруспеков

Е.

Turuspekov

yerlant@yahoo.com

Институт биологии и биотехнологии растений Комитета науки Министерства образования и науки Республики Казахстан; Казахский национальный аграрный университетРоссияInstitute of Plant Biology and Biotechnology; Kazakh National Agrarian UniversityRussian Federation

Институт биологии и биотехнологии растений Комитета науки Министерства образования и науки Республики КазахстанРоссияInstitute of Plant Biology and BiotechnologyRussian Federation

Казахский научно-исследовательский институт земледелия и растениеводстваРоссияKazakh Research Institute of Agriculture and Plant GrowingRussian Federation

Научно-исследовательский институт проблем биологической безопасности Комитета науки Министерства образования и науки Республики КазахстанРоссияResearch Institute for Biological Safety ProblemsRussian Federation

2018

09082018

225536543

2018

Затыбеков А., Абугалиева С., Дидоренко С., Рсалиев А., Туруспеков Е.

Zatybekov A., Abugalieva S., Didorenko S., Rsaliyev A., Turuspekov Y.

This work is licensed under a Creative Commons Attribution 4.0 License.

https://vavilov.elpub.ru/jour/article/view/1593

Соя (Glycine max (L.) Merr) – важная пищевая, кормовая и техническая культура. В Казахстане площадь под соей увеличивается с каждым годом, что обусловлено ее важностью в решении проблемы дефицита белка в питании людей и кормлении животных. Одной из основных проблем производства сои являются грибные болезни, вызывающие потери урожая до 30 %. Для повышения эффективности селекции, направленной на улучшение устойчивости сои к болезням, могут быть использованы современные геномные технологии. Таким образом, целью настоящего исследования был полногеномный анализ ассоциаций (GWAS) в коллекции сои, состоящей из 182 образцов, на устойчивость к грибным болезням в условиях Юго-Восточного и Южного Казахстана. В результате полевой оценки коллекции сои обнаружены растения, пораженные Fusarium spp. и Cercospora sojina в Южном регионе (НИИПББ) и Septoria glycines – в Юго-Восточном регионе (КазНИИЗиР). Исследование было нацелено на идентификацию локусов количественных признаков (ЛКП), связанных с устойчивостью к основным заболеваниям, таким как фузариоз корневой гнили (FUS), церкоспороз (FLS) и септориоз (BS). GWAS с использованием 4 442 SNP-маркеров (single nucleotide polymorphism) матрицы Illumina iSelect позволил идентифицировать 15 ассоциаций маркер – признак (MTA) на устойчивость к трем болезням на двух разных стадиях роста. Генетически картированы два ЛКП как для FUS (хромосомы 13 и 17), так и для BS (хромосомы 14 и 17), включая один предположительно новый ЛКП для BS, который был идентифицирован на хромосоме 17. Кроме того, пять предположительно новых ЛКП для FLS были идентифицированы на хромосомах сои 2, 7 и 15. Результаты исследования могут быть использованы для улучшения селекционных программ, в том числе маркер-опосредованной селекции.

Soybean (Glycine max (L.) Merr) is an essential food, feed, and technical culture. In Kazakhstan the area under soybean is increasing every year, helping to solve the problem of protein deficiency in human nutrition and animal feeding. One of the main problems of soybean production is fungal diseases causing yields losses of up to 30 %. Modern genomic studies can be applied to facilitate efficient breeding research for improvement of soybean fungal disease tolerance. Therefore, the objective of this genome-wide association study (GWAS) was analysis of a soybean collection consisting of 182 accessions in relation to fungal diseases in the conditions of South East and South Kazakhstan. Field evaluation of the soybean collection suggested that Fusarium spp. and Cercospora sojina affected plants in the South region (RIBSP), and Septoria glycines – in the South East region (KRIAPP). The major objective of the study was identification of QTL associated with resistance to fusarium root rot (FUS), frogeye leaf spot (FLS), and brown spot (BS). GWAS using 4 442 SNP (single nucleotide polymorphism) markers of Illumina iSelect array allowed for identification of fifteen marker trait associations (MTA) resistant to the three diseases at two different stages of growth. Two QTL both for FUS (chromosomes 13 and 17) and BS (chromosomes 14 and 17) were genetically mapped, including one presumably novel QTL for BS (chromosome 17). Also, five presumably novel QTL for FLS were genetically mapped on chromosomes 2, 7, and 15. The results can be used for improvement of the local breeding projects based on marker-assisted selection approach.

сояфузариоз корневой гнилицеркоспорозсепториозGWASSNPЛКП картирование

soybeanfusarium root rotfrogeye leaf spotbrown spotGWASSNPQTL mapping

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The authors declare that there are no conflicts of interest present.