vavilovВавиловский журнал генетики и селекцииVavilov Journal of Genetics and Breeding2500-3259Institute of Cytology and Genetics of Siberian Branch of the RAS10.18699/VJ18.395vavilov-1594Research ArticleГЕНОФОНД И СЕЛЕКЦИЯ РАСТЕНИЙPLANT GENE POOL AND BREEDINGТенденция преодоления устойчивости к бурой ржавчине интрогрессивных линий мягкой пшеницы с генетическим материалом Aegilops speltoides TauschA tendency towards leaf rust resistance decrease in common wheat introgression lines with genetic material from Aegilops speltoides TauschПлотниковаЛ. Я.PlotnikovaL. Ya.lya.plotnikova@omgau.orgМешковаЛ. В.MeshkovaL. V.ГультяеваЕ. И.GultyaevaE. I.МитрофановаО. П.MitrofanovaO. Р.ЛапочкинаИ. Ф.LapochkinaI. F.Омский государственный аграрный университет имени П.А. СтолыпинаРоссияOmsk State Agrarian University named after P.A. StolypinRussian FederationОмский аграрный научный центрРоссияOmsk Agrarian Scientific CenterRussian FederationВсероссийский научно-исследовательский институт защиты растенийРоссияAll-Russian Institute of Plant ProtectionRussian FederationФедеральный исследовательский центр Всероссийский институт генетических ресурсов растений имени Н.И. Вавилова (ВИР)РоссияFederal Research Center the N.I. Vavilov All-Russian Institute of Plant Genetic Resources (VIR)Russian FederationМосковский научно-исследовательский институт сельского хозяйства (Немчиновка)РоссияMoscow Agricultural Research Institute (Nemchinovka)Russian Federation201809082018225560567Copyright © Плотникова Л.Я., Мешкова Л.В., Гультяева Е.И., Митрофанова О.П., Лапочкина И.Ф., 20182018Плотникова Л.Я., Мешкова Л.В., Гультяева Е.И., Митрофанова О.П., Лапочкина И.Ф.Plotnikova L.Y., Meshkova L.V., Gultyaeva E.I., Mitrofanova O.Р., Lapochkina I.F.This work is licensed under a Creative Commons Attribution 4.0 License.https://vavilov.elpub.ru/jour/article/view/1594

Развитие бурой ржавчины, вызываемой грибом Puccinia triticina Erikss., приводит к существенным потерям урожая мягкой пшеницы Triticum aestivum L. Вид Aegilops speltoides Tausch (2n = 14, геном SS) считается перспективным источником генов для защиты пшеницы от болезней. Целью исследований был мониторинг устойчивости образцов Ae. speltoides, интрогрес­сивных линий и сортов мягкой пшеницы с генетическим мате­риалом этого вида к западносибирской популяции возбудите­ля бурой ржавчины. Оценка устойчивости растений в условиях естественного инфекционного фона показала, что образцы Ae. speltoides были иммунны к бурой ржавчине, однако наблю­дается тенденция повышения восприимчивости интрогрессив­ных линий и сортов пшеницы. Отмечено снижение защитного действия генов Lr28, Lr36 и Lr35, но Lr47 сохраняет свою эффективность в западносибирском регионе, что подтверждено оценкой вирулентности омской популяции P. triticina к пере­численным генам. В период исследований (2003–2017 гг.) была преодолена резистентность линий Од 26/89, 156/90, аналогов сорта Новосибирская 67 АНК-39 (В, С) и линии Л-500 из коллек­ции «Арсенал». Линии Од (35/1, 35/89, 210/90, 278/89), АНК-39 (А, D, E), Л-501, сорта Челяба 75 и Mit оставались высокоустойчивыми в течение всего периода проведения испытаний. По данным молекулярного анализа и фитопатологического тести­рования, в изученных образцах (за исключением линий сорта Thatcher и Pavon) не выявлены известные гены Lr28, Lr36 и Lr47 от Ae. speltoides. На основании данных, полученных с помощью ДНК-маркеров, сделано предположение о том, что сорт Челяба 75, семь устойчивых линий серий Од и АНК-39 содержат транслокации с геном LrSp. В остальных образцах вероятно наличие дополнительных неидентифицированных генов устойчивости Ae. speltoides. Тенденцию снижения устойчивости к бурой ржавчине интрогрессивных линий и сортов с генами Ae. speltoides необходимо учитывать при селекции мягкой пше­ницы, предназначенной для выращивания в Западной Сибири.

Leaf rust, caused by the fungus Puccinia triticina Erikss., inflicts serious crop loss of common wheat Triticum aesti­vum L. The species Aegilops speltoides Tausch (2n = 14, SS) is considered a promising issue of genes to protect bread wheat from diseases. The objective of this study was the monitoring of resistance to leaf rust of Ae. speltoides ac­cessions and introgressive lines and cultivars with genetic material of this species to the Western Siberian popula­tion of fungus. The estimation of specimens in the field conditions on natural infectious background showed that the Ae. speltoides accessions were immune to leaf rust, however, а tendency towards increasing susceptibility of the introgressive lines and varieties was detected. The protective effect of the known genes Lr28, Lr36 and Lr35 decreased, but Lr47 remained efficient in West Siberia, as confirmed by the results of testing of the Omsk population of P. triticina for virulence to the mentioned genes. During the study (2003-2017) the resistance has been overcome of lines Od 26/89, 156/90, analogs of cv. Novosibirskaya 67 – ANK-39 (B, C), and L-500 from the “Arsenal” сollection. High resistance to leaf rust was preserved of lines Od (35/1, 35/89, 210/90, 278/89); ANK39 (A, D, E); L-501 and cvs. Chelyaba 75 and Mit. Analysis of DNA markers and phytopathological tests showed that the studied variet­ies and lines lacked the known genes Lr28, Lr36, and Lr47 from Ae. speltoides (except for the lines of cvs. Thatcher and Pavon). On the base of analysis of DNA markers, it was assumed that Chelyaba 75 and seven resistant lines from the Odand ANK-39-series have translocations bearing the LrSp gene. Presumably, the rest of samples possess additional not yet identified genes of Ae. speltoides. The trend of overcoming of resistance to leaf rust of introgres­sive lines and varieties with Ae. speltoides genes should be taken into consideration in common wheat breeding in Western Siberia.

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A Thinopyrum intermedium chromosome in bread wheat cultivars as a source of genes conferring resistance to fungal diseases. Euphytica. 2015;204:91-101. DOI 10.1007/s10681014-1344-5.Tyunin V.А., Shreyder Е.R., Gultyaeva E.I., Shaydayuk E.L. Characteristics of virulence of Puccinia triticina populations and the potential of the Lr24, Lr25, LrSp genes for spring common wheat breeding in the Southern Ural. Vavilovskii Zhurnal Genetiki i Selektsii = Vavilov Journal of Genetics and Breeding. 2017;21(5):523529. DOI 10.18699/VJ17.269. (in Russian)Sanin S.S. Phytosanitary monitoring: current state and ways of improvement. Problems of Optimization of the Phytosanitary Condition of Plant Growing. Proceedings of the All-Russian Congress on Plant Protection, St. Petersburg, December 1995. St. Petersburg, 1997;166-176. (in Russian)Volkova G.V., Kremneva O.Yu., Shumilov Yu.V., Gladkova E.V., Vaganova O.F., Mitrofanova O.P., Lysenko N.S., Chikida N.N., Khakimova A.G., Zuev E.V. Immunologic estimation of accessions of wheat, its rare species, and Aegilops from the collection of N.I. Vavilov All-Russian Institute of Plant Genetic Resources and selection of sources with group resistance. Vestnik Zaschity Rastenii = Bulletin of Plant Protection. 2016;89(3):38-39. (in Russian)Sanin S.S. Phytosanitary monitoring: current state and ways of improvement. Problems of Optimization of the Phytosanitary Condition of Plant Growing. Proceedings of the All-Russian Congress on Plant Protection, St. Petersburg, December 1995. St. Petersburg, 1997;166-176. (in Russian)Voronkova A.A., Dubonosov T.S., Panarin I.V. Causes of leaf rust outbreaks in the Krasnodar region. Rust of Cereals. Moscow: Kolos Publ., 1975;80-88. (in Russian)Shah T.M., Ahmed J., Asghar M., Iqbal N., Farooq S. Evaluation of annual wild grass species for leaf rust resistance. Pak. J. Biol. Sci. 2000;3(3):469-472.Shah T.M., Ahmed J., Asghar M., Iqbal N., Farooq S. Evaluation of annual wild grass species for leaf rust resistance. Pak. J. Biol. Sci. 2000;3(3):469-472.Shah T.M., Ahmed J., Asghar M., Iqbal N., Farooq S. Evaluation of annual wild grass species for leaf rust resistance. Pak. J. Biol. Sci. 2000;3(3):469-472.Sibikeev S.N., Voronina S.A., Badaeva E.D., Druzhin A.E. Study of resistance to leaf and stem rust in Triticum aestivum – Aegilops speltoides lines. Vavilovskii Zhurnal Genetiki i Selektsii = Vavilov Journal of Genetics and Breeding. 2015;19(2):165-170. (in Russian)Sibikeev S.N., Voronina S.A., Badaeva E.D., Druzhin A.E. Study of resistance to leaf and stem rust in Triticum aestivum – Aegilops speltoides lines. Vavilovskii Zhurnal Genetiki i Selektsii = Vavilov Journal of Genetics and Breeding. 2015;19(2):165-170. (in Russian)Sibikeev S.N., Voronina S.A., Badaeva E.D., Druzhin A.E. Study of resistance to leaf and stem rust in Triticum aestivum – Aegilops speltoides lines. Vavilovskii Zhurnal Genetiki i Selektsii = Vavilov Journal of Genetics and Breeding. 2015;19(2):165-170. (in Russian)Sibikeev S.N., Voronina S.A., Badaeva E.D., Druzhin A.E. Study of resistance to leaf and stem rust in Triticum aestivum – Aegilops speltoides lines. Vavilovskii Zhurnal Genetiki i Selektsii = Vavilov Journal of Genetics and Breeding. 2015;19(2):165-170. (in Russian)Sochalova L.P. Sources of wheat resistance genes to leaf and stem pathogens in the Novosibirsk region. Zernovoe Khozjaistvo Rossii = Grain Economy of Russia. 2016;2:45-49. (in Russian)Sochalova L.P. Sources of wheat resistance genes to leaf and stem pathogens in the Novosibirsk region. Zernovoe Khozjaistvo Rossii = Grain Economy of Russia. 2016;2:45-49. (in Russian)Sochalova L.P. Sources of wheat resistance genes to leaf and stem pathogens in the Novosibirsk region. Zernovoe Khozjaistvo Rossii = Grain Economy of Russia. 2016;2:45-49. (in Russian)Sochalova L.P. Sources of wheat resistance genes to leaf and stem pathogens in the Novosibirsk region. Zernovoe Khozjaistvo Rossii = Grain Economy of Russia. 2016;2:45-49. (in Russian)Sochalova L.P., Piskarev V.V. Resistance of spring common wheat varieties to agents of infectious diseases in the changing climate of West Siberia. Dostizheniya Nauki i Tekhniki APK = Achievements of Sci-ence and Techniques of Agroindustrial Complex. 2017;31(2):21-25. (in Russian)Sochalova L.P., Piskarev V.V. Resistance of spring common wheat varieties to agents of infectious diseases in the changing climate of West Siberia. Dostizheniya Nauki i Tekhniki APK = Achievements of Sci-ence and Techniques of Agroindustrial Complex. 2017;31(2):21-25. (in Russian)Sochalova L.P., Piskarev V.V. Resistance of spring common wheat varieties to agents of infectious diseases in the changing climate of West Siberia. Dostizheniya Nauki i Tekhniki APK = Achievements of Sci-ence and Techniques of Agroindustrial Complex. 2017;31(2):21-25. (in Russian)Sochalova L.P., Piskarev V.V. Resistance of spring common wheat varieties to agents of infectious diseases in the changing climate of West Siberia. Dostizheniya Nauki i Tekhniki APK = Achievements of Sci-ence and Techniques of Agroindustrial Complex. 2017;31(2):21-25. (in Russian)Tyryshkin L.G., Syukov V.V., Zakharov V.G., Zuev E.V., Gashimov M.E., Kolesova M.A., Chikida N.N., Yershova M.A., Belousova M.H. Sources of effective resistance of common wheat and its relatives to fungal diseases: search, creation, and use in breeding. Trudy po Prikladnoy Botanike, Genetike i Selektzii = Proceedings on Applied Botany, Genetics, and Breeding. 2012; 170:187-201. (in Russian)Tyryshkin L.G., Syukov V.V., Zakharov V.G., Zuev E.V., Gashimov M.E., Kolesova M.A., Chikida N.N., Yershova M.A., Belousova M.H. Sources of effective resistance of common wheat and its relatives to fungal diseases: search, creation, and use in breeding. Trudy po Prikladnoy Botanike, Genetike i Selektzii = Proceedings on Applied Botany, Genetics, and Breeding. 2012; 170:187-201. (in Russian)Tyryshkin L.G., Syukov V.V., Zakharov V.G., Zuev E.V., Gashimov M.E., Kolesova M.A., Chikida N.N., Yershova M.A., Belousova M.H. Sources of effective resistance of common wheat and its relatives to fungal diseases: search, creation, and use in breeding. Trudy po Prikladnoy Botanike, Genetike i Selektzii = Proceedings on Applied Botany, Genetics, and Breeding. 2012; 170:187-201. (in Russian)Tyryshkin L.G., Syukov V.V., Zakharov V.G., Zuev E.V., Gashimov M.E., Kolesova M.A., Chikida N.N., Yershova M.A., Belousova M.H. Sources of effective resistance of common wheat and its relatives to fungal diseases: search, creation, and use in breeding. Trudy po Prikladnoy Botanike, Genetike i Selektzii = Proceedings on Applied Botany, Genetics, and Breeding. 2012; 170:187-201. (in Russian)Tyunin V.А., Shreyder Е.R., Gultyaeva E.I., Shaydayuk E.L. Characteristics of virulence of Puccinia triticina populations and the potential of the Lr24, Lr25, LrSp genes for spring common wheat breeding in the Southern Ural. Vavilovskii Zhurnal Genetiki i Selektsii = Vavilov Journal of Genetics and Breeding. 2017;21(5):523529. DOI 10.18699/VJ17.269. (in Russian)Tyunin V.А., Shreyder Е.R., Gultyaeva E.I., Shaydayuk E.L. Characteristics of virulence of Puccinia triticina populations and the potential of the Lr24, Lr25, LrSp genes for spring common wheat breeding in the Southern Ural. Vavilovskii Zhurnal Genetiki i Selektsii = Vavilov Journal of Genetics and Breeding. 2017;21(5):523529. DOI 10.18699/VJ17.269. (in Russian)Tyunin V.А., Shreyder Е.R., Gultyaeva E.I., Shaydayuk E.L. Characteristics of virulence of Puccinia triticina populations and the potential of the Lr24, Lr25, LrSp genes for spring common wheat breeding in the Southern Ural. Vavilovskii Zhurnal Genetiki i Selektsii = Vavilov Journal of Genetics and Breeding. 2017;21(5):523529. DOI 10.18699/VJ17.269. (in Russian)Tyunin V.А., Shreyder Е.R., Gultyaeva E.I., Shaydayuk E.L. Characteristics of virulence of Puccinia triticina populations and the potential of the Lr24, Lr25, LrSp genes for spring common wheat breeding in the Southern Ural. Vavilovskii Zhurnal Genetiki i Selektsii = Vavilov Journal of Genetics and Breeding. 2017;21(5):523529. DOI 10.18699/VJ17.269. (in Russian)Volkova G.V., Kremneva O.Yu., Shumilov Yu.V., Gladkova E.V., Vaganova O.F., Mitrofanova O.P., Lysenko N.S., Chikida N.N., Khakimova A.G., Zuev E.V. Immunologic estimation of accessions of wheat, its rare species, and Aegilops from the collection of N.I. Vavilov All-Russian Institute of Plant Genetic Resources and selection of sources with group resistance. Vestnik Zaschity Rastenii = Bulletin of Plant Protection. 2016;89(3):38-39. (in Russian)Volkova G.V., Kremneva O.Yu., Shumilov Yu.V., Gladkova E.V., Vaganova O.F., Mitrofanova O.P., Lysenko N.S., Chikida N.N., Khakimova A.G., Zuev E.V. Immunologic estimation of accessions of wheat, its rare species, and Aegilops from the collection of N.I. Vavilov All-Russian Institute of Plant Genetic Resources and selection of sources with group resistance. Vestnik Zaschity Rastenii = Bulletin of Plant Protection. 2016;89(3):38-39. (in Russian)Volkova G.V., Kremneva O.Yu., Shumilov Yu.V., Gladkova E.V., Vaganova O.F., Mitrofanova O.P., Lysenko N.S., Chikida N.N., Khakimova A.G., Zuev E.V. Immunologic estimation of accessions of wheat, its rare species, and Aegilops from the collection of N.I. Vavilov All-Russian Institute of Plant Genetic Resources and selection of sources with group resistance. Vestnik Zaschity Rastenii = Bulletin of Plant Protection. 2016;89(3):38-39. (in Russian)Volkova G.V., Kremneva O.Yu., Shumilov Yu.V., Gladkova E.V., Vaganova O.F., Mitrofanova O.P., Lysenko N.S., Chikida N.N., Khakimova A.G., Zuev E.V. Immunologic estimation of accessions of wheat, its rare species, and Aegilops from the collection of N.I. Vavilov All-Russian Institute of Plant Genetic Resources and selection of sources with group resistance. Vestnik Zaschity Rastenii = Bulletin of Plant Protection. 2016;89(3):38-39. (in Russian)Voronkova A.A., Dubonosov T.S., Panarin I.V. Causes of leaf rust outbreaks in the Krasnodar region. Rust of Cereals. Moscow: Kolos Publ., 1975;80-88. (in Russian)Voronkova A.A., Dubonosov T.S., Panarin I.V. Causes of leaf rust outbreaks in the Krasnodar region. Rust of Cereals. Moscow: Kolos Publ., 1975;80-88. (in Russian)Voronkova A.A., Dubonosov T.S., Panarin I.V. Causes of leaf rust outbreaks in the Krasnodar region. Rust of Cereals. Moscow: Kolos Publ., 1975;80-88. (in Russian)Voronkova A.A., Dubonosov T.S., Panarin I.V. Causes of leaf rust outbreaks in the Krasnodar region. Rust of Cereals. Moscow: Kolos Publ., 1975;80-88. (in Russian)

The authors declare that there are no conflicts of interest present.