vavilovВавиловский журнал генетики и селекцииVavilov Journal of Genetics and Breeding2500-3259Institute of Cytology and Genetics of Siberian Branch of the RASvavilov-160Research ArticleСтатьиArticlesГЕНЕТИЧЕСКИЙ И МОЛЕКУЛЯРНЫЙ АНАЛИЗ КОМПЛЕМЕНТАЦИИ ЛОКУСОВ FLAMENCO И PIWI У DROSOPHILA MELANOGASTERGENETIC AND MOLECULAR ANALYSIS OF COMPLEMENTATION OF THE FLAMENCO AND PIWI LOCI IN DROSOPHILA MELANOGASTERУрусовФ. А.UrusovF. A.aikim57@mail.ruНефедоваЛ. Н.NefedovaL. N.aikim57@mail.ruЛавреновА. Р.LavrenovA. R.aikim57@mail.ruРомановаН. И.RomanovaN. I.aikim57@mail.ruКимА. И.KimA. I.aikim57@mail.ruМосковский государственный университет имени М.В. Ломоносова, кафедра генетики, Москва, РоссияРоссияM.V. Lomonosov Moscow State University, Department of Genetics, Moscow, RussiaRussian Federation201328122014173381389Copyright © Урусов Ф.А., Нефедова Л.Н., Лавренов А.Р., Романова Н.И., Ким А.И., 20152015Урусов Ф.А., Нефедова Л.Н., Лавренов А.Р., Романова Н.И., Ким А.И.Urusov F.A., Nefedova L.N., Lavrenov A.R., Romanova N.I., Kim A.I.This work is licensed under a Creative Commons Attribution 4.0 License.https://vavilov.elpub.ru/jour/article/view/160

Считается, что гетерохроматиновый локус flamenco, контролирующий у Drosophila melanogaster транспозиции ретротранспозона/ретровируса gypsy, является одним из источников антисмысловых РНК, взаимодействующих с белком Piwi –важнейшим компонентом системы РНК-интерференции. Мутации piwi и flamenco имеют одинаковое фенотипическое проявление –повышенный уровень транскрипции и частоты транспозиции ретротранспозона/ретровируса gypsy. В настоящей работе обсуждаются результаты комплементационного теста, который заключается в скрещивании линий MS и SS, мутантных по локусу flamenco, с линией piwi3, гетерозиготной по мутации в гене piwi, с последующим исследованием уровня транскрипции gypsy в яичниках и семенниках гибридов, полученных от данных скрещиваний. Проведен генетический (гибридологический) и молекулярный анализы взаимодействия локусов flamenco и piwi. Выявлено, что транскрипция gypsy по-разному регулируется в тканях семенников и яичников линий SS и MS, что, по-видимому, обусловлено различной активностью кластеров piRNA в этих тканях. Также показано, что гены piwi и flamenco в тканях семенников взаимодействуют комплементарно.

It is believed that the heterochromatic locus flamenco, controlling transposition of retrotransposon/retrovirus gypsy in Drosophila melanogaster, is a source of Piwi-interacting RNA. Piwi is the primary component of the RNA interference machinery. Mutations in piwi and flamenco have the same phenotype – an enhanced transcription and frequency of transposition of the retrotransposon/retrovirus gypsy. This paper discusses the results of the complementation test, which involves crossing strains MS and SS, mutant for the flamenco locus, with the piwi strain 3, heterozygous for a mutation in the piwi gene, followed by study of the transcription level of gypsy in the ovaries and testes of hybrids derived from these crosses. Genetic (hybridological) and molecular analysis of the interaction of the flamenco and piwi loci has been conducted. It has been revealed that transcription of gypsy is differently regulated in testis and ovaries of strains SS and MS, apparently owing to the different activity of piRNA clusters in these tissues. It has been also shown that the piwi and flamenco genes interact complementarily in testis tissue.

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The authors declare that there are no conflicts of interest present.