vavilovВавиловский журнал генетики и селекцииVavilov Journal of Genetics and Breeding2500-3259Institute of Cytology and Genetics of Siberian Branch of the RASvavilov-162Research ArticleСтатьиArticlesОСОБЕННОСТИ КРИВОЙ ВЫЖИВАНИЯ У DROSOPHILA MELANOGASTER СО СВЕРХЭКСПРЕССИЕЙ ГЕНА D-GADD45SURVIVAL CURVE ANALYSIS FOR DROSOPHILA MELANOGASTER WITH D-GADD45 OVEREXPRESSION IN THE NERVOUS SYSTEMПлюснинаЕ. Н.PlyusninaE. N.ome@mcb.nsc.ruШапошниковМ. В.ShaposhnikovM. V.ome@mcb.nsc.ruАндрееваЕ. Н.AndreevaE. N.МоскалевА. А.MoskalevA. A.ome@mcb.nsc.ruОмельянчукЛ. В.OmelyanchukL. V.Федеральное государственное бюджетное учреждение науки Институт биологии Коми научного центра Уральского отделения Российской академии наук, Сыктывкар, Россия ФГБОУ ВПО Сыктывкарский государственный университет, Сыктывкар, РоссияРоссияInstitute of Biоlogy, Komi Center of UB RAS, Syktivkar, Russia Syktivkar State University, RussiaRussian FederationФедеральное государственное бюджетное учреждение науки Институт молекулярной и клеточной биологии Сибирского отделения Российской академии наук, Новосибирск, РоссияРоссияInstitute of Molecular and Cellular Biology SB RAS, Novosibirsk, RussiaRussian FederationФедеральное государственное бюджетное учреждение науки Институт биологии Коми научного центра Уральского отделения Российской академии наук, Сыктывкар, Россия ФГБОУ ВПО Сыктывкарский государственный университет, Сыктывкар, Россия Московский физико-технический институт (государственный университет), Москва, РоссияРоссияInstitute of Biоlogy, Komi Center of UB RAS, Syktivkar, Russia Syktivkar State University, Russia Moscow Institute of Physics and Technology, RussiaRussian Federation201328122014173399403Copyright © Плюснина Е.Н., Шапошников М.В., Андреева Е.Н., Москалев А.А., Омельянчук Л.В., 20152015Плюснина Е.Н., Шапошников М.В., Андреева Е.Н., Москалев А.А., Омельянчук Л.В.Plyusnina E.N., Shaposhnikov M.V., Andreeva E.N., Moskalev A.A., Omelyanchuk L.V.This work is licensed under a Creative Commons Attribution 4.0 License.https://vavilov.elpub.ru/jour/article/view/162

Известно, что сверхэкспрессия гена Gadd45 в нервной системе дрозофилы увеличивает продолжительность жизни. В работе проведена аппроксимация кривых выживания дрозофил в такого рода экспериментах с помощью распределения Гомпертца и показано, что продление жизни достигается за счет изменения параметра R, отражающего интенсивность гибели в начальный момент времени, а не за счет другого параметра распределения Гомпертца, α, отражающего экспоненциально растущую интенсивность гибели. Дополнительно провели анализ кривых выживания для случая радиационного гормезиса (когда облучение малыми дозами радиации вызывает продление жизни особей) и также подтвердили найденную в первом случае закономерность. На этой основе заключили, что поиск геропротекторов перспективно проводить среди веществ, уменьшающих параметр R, который более легко измерим,чем полная кривая выживания.

Overexpression of the Gadd45 gene in the nervous system of Drosophila melanogaster increases the life span. We have performed the Gompertz approximation of the survival curve and revealed a life span increase as a result of decrease of the R parameter, reflecting the death level at the beginning of adult life, whereas the other Gompertz parameter, α, responsible for final stage of the life, does not change essentially. In addition, we have analyzed the case of radiation hormesis (low doses of radiation increase life span) and found a similar effect. We conclude that it is promising to perform geroprotector screen by testing substances for the ability to decrease R. Its measurement is less labor-consuming than the construction of the complete survival curve.

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The authors declare that there are no conflicts of interest present.