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<article article-type="research-article" dtd-version="1.3" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:lang="ru"><front><journal-meta><journal-id journal-id-type="publisher-id">vavilov</journal-id><journal-title-group><journal-title xml:lang="ru">Вавиловский журнал генетики и селекции</journal-title><trans-title-group xml:lang="en"><trans-title>Vavilov Journal of Genetics and Breeding</trans-title></trans-title-group></journal-title-group><issn pub-type="epub">2500-3259</issn><publisher><publisher-name>Institute of Cytology and Genetics of Siberian Branch of the RAS</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.18699/VJ18.404</article-id><article-id custom-type="elpub" pub-id-type="custom">vavilov-1646</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="ru"><subject>ГЕНЕТИЧЕСКИЕ РЕСУРСЫ И БИОКОЛЛЕКЦИИ</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="en"><subject>PLANT GENETICS</subject></subj-group></article-categories><title-group><article-title>SSR-анализ геномной ДНК перспективных сортов мягкой озимой пшеницы узбекистанской селекции</article-title><trans-title-group xml:lang="en"><trans-title>SSR analysis of the genomic DNA of perspective  Uzbek hexaploid winter wheat varieties</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Адылова</surname><given-names>А. Т.</given-names></name><name name-style="western" xml:lang="en"><surname>Adylova</surname><given-names>A. T.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Ташкент.</p></bio><bio xml:lang="en"><p>Tashkent.</p></bio><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Норбеков</surname><given-names>Ж. К.</given-names></name><name name-style="western" xml:lang="en"><surname>Norbekov</surname><given-names>G. K.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Ташкент.</p></bio><bio xml:lang="en"><p>Tashkent.</p></bio><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Хуршут</surname><given-names>Э. Э.</given-names></name><name name-style="western" xml:lang="en"><surname>Khurshut</surname><given-names>E. E.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Ташкент.</p></bio><bio xml:lang="en"><p>Tashkent.</p></bio><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Никитина</surname><given-names>Е. В.</given-names></name><name name-style="western" xml:lang="en"><surname> Nikitina</surname><given-names>E. V.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Ташкент.</p></bio><bio xml:lang="en"><p>Tashkent.</p></bio><email xlink:type="simple">elenanikita2013@rambler.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Кушанов</surname><given-names>Ф. Н.</given-names></name><name name-style="western" xml:lang="en"><surname>Kushanov</surname><given-names>F. N.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Ташкент.</p></bio><bio xml:lang="en"><p>Tashkent.</p></bio><xref ref-type="aff" rid="aff-1"/></contrib></contrib-group><aff-alternatives id="aff-1"><aff xml:lang="ru">Центр геномики и биоинформатики Академии наук Республики Узбекистан.<country>Узбекистан</country></aff><aff xml:lang="en">Center of Genomics and Bioinformatics of the Academy of Sciences of the Republic of Uzbekistan.<country>Uzbekistan</country></aff></aff-alternatives><pub-date pub-type="collection"><year>2018</year></pub-date><pub-date pub-type="epub"><day>24</day><month>09</month><year>2018</year></pub-date><volume>22</volume><issue>6</issue><fpage>634</fpage><lpage>639</lpage><permissions><copyright-statement>Copyright &amp;#x00A9; Адылова А.Т., Норбеков Ж.К., Хуршут Э.Э., Никитина Е.В., Кушанов Ф.Н., 2018</copyright-statement><copyright-year>2018</copyright-year><copyright-holder xml:lang="ru">Адылова А.Т., Норбеков Ж.К., Хуршут Э.Э., Никитина Е.В., Кушанов Ф.Н.</copyright-holder><copyright-holder xml:lang="en">Adylova A.T., Norbekov G.K., Khurshut E.E.,  Nikitina E.V., Kushanov F.N.</copyright-holder><license license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>This work is licensed under a Creative Commons Attribution 4.0 License.</license-p></license></permissions><self-uri xlink:href="https://vavilov.elpub.ru/jour/article/view/1646">https://vavilov.elpub.ru/jour/article/view/1646</self-uri><abstract><p>Целью работы было изучение генетического разнообразия сортов мягкой пшеницы узбекистанской селекции, так как они являются источником адаптированного к местным условиям обитания растительного материала и могут служить важнейшим поставщиком генетических ресурсов для селекционных работ по пшенице не только в Узбекистане, но и в других странах. В настоящее время микросателлитные маркеры (simple sequence repeats, SSR –  простые повторяющиеся последовательности) – одни из наиболее широко используемых и эффективных классов ДНК-маркеров для генотипирования, паспортизации и классификации сортов растений. В работе представлены результаты генотипирования 32 сортов мягкой пшеницы отечественной селекции с использованием 144 микро- сателлитных праймерных пар, выбранных исходя из литературных данных, 36 пар из них дали полиморфные хорошо воспроизводимые ПЦР-фрагменты. Для каждого сорта были получены индивидуальные SSR-спектры, различающиеся числом ампликонов. По 36 микросател- литным локусам выявлен 141 аллель, их число на локус (Na) составляло от 2 до 6 (3 в среднем). Для изученной группы генотипов эффективное число аллелей (ne), характеризующее локусы по частоте встречаемости аллелей, варьировало от 1.7 до 4.8, составляя в среднем 2.8. Величина ожидаемой гетерозиготности (He) в нашей популяции пшеницы была в среднем 0.626, меняясь от 0 до 0.792. Размеры амплифицированных продуктов находились в пределах от 93 до 552 п. н. Индекс полиморфного информационного содержания (PIC) варьировал от 0 до 0.758. На основании набора аллелей микросателлитных локусов была построена дендрограмма, отражающая филогенетические различия изученных сортов мягкой пшеницы, которая показала, что сорта узбекистанской селекции разделяются на два больших кластера, это свидетельствует о возможной общности их происхождения. Для каждого сорта пшеницы Узбекистана разработана генетическая формула, которая может быть использована для идентификации, паспортизации этих сортов, а также при подборе родительских пар в селекционных программах по пшенице.</p></abstract><trans-abstract xml:lang="en"><p>The objective of this study was to investigate the genetic diversity of hexaploid wheat varieties of Uzbekistan breeding using simple sequence repeat (SSR) markers. These varieties are adapted to local conditions, and can be considered as the most important supplier of genetic resources for cultivation in Uzbekistan and other countries. Microsatellite markers are now most widely used and effective classes of DNA markers for genotyping, certification and classification of plant varieties. In this paper, genotyping results of 32 hexaploid wheat domestic varieties using 144 microsatellite primer pairs are presented. Microsatellite primer pairs were chosen from literature data and 36 primer pairs (from 144) gave polymorphic well-reproducible PCR-fragments. The individual SSR spectra differing in number of amplicons were obtained for each variety. A total number of 141 alleles for 36 microsatellite loci were detected. The number of alleles per locus ranged from 2 to 6, the mean number of alleles per locus (Na) was 3 alleles. For the studied genotypes group the effective number of alleles (ne) characterizing the loci by the allele frequency, varied from 1.7 to 4.8, the mean number of alleles per locus was 2.8. The expected heterozygosity (He) ranged from 0 to 0.792, averaging 0.626, in studied wheat population. The amplified fragment sizes ranged from 93 to 552 bp. The polymorphic index content (PIC) ranged from 0 to 0.758. A dendrogram was constructed using the alleles set of microsatellite loci, reflecting the phylogenetic differences of the studied hexaploid wheat varieties. It showed that Uzbekistan breeding varieties are divided into two main clusters, which may be evidence of their common origin.  A genetic formula has been developed for each Uzbek wheat variety. It can be used for identification, certification of these varieties, as well as for the selection of parental pairs in the wheat breeding programs.</p></trans-abstract><kwd-group xml:lang="ru"><kwd>мягкая озимая пшеница</kwd><kwd>ПЦР-анализ</kwd><kwd>генетическое разнообразие</kwd><kwd>микросателлитные локусы ДНК</kwd><kwd>кластеризация</kwd><kwd>паспортизация</kwd></kwd-group><kwd-group xml:lang="en"><kwd>hexaploid winter wheat</kwd><kwd>PCR analysis</kwd><kwd>genetic diversity</kwd><kwd>microsatellite DNA loci</kwd><kwd>clusterization</kwd><kwd>barcoding</kwd></kwd-group></article-meta></front><back><ref-list><title>References</title><ref id="cit1"><label>1</label><citation-alternatives><mixed-citation xml:lang="ru">Ayala F., Kiger J. Modern Genetics. Menlo Park, Calif.: Benjamin/ Cummings Publ. Co., 1984.</mixed-citation><mixed-citation xml:lang="en">Ayala F., Kiger J. Modern Genetics. Menlo Park, Calif.: Benjamin/ Cummings Publ. Co., 1984.</mixed-citation></citation-alternatives></ref><ref id="cit2"><label>2</label><citation-alternatives><mixed-citation xml:lang="ru">Ches nokov Yu.V., Artemyeva A.M. Evaluation of the measure of polymorphism information of genetic diversity. Selskokhozyaystvennaya Biologiya = Agricultural Biology. 2015;50(5):571578. (in Rus sian)</mixed-citation><mixed-citation xml:lang="en">Ches nokov Yu.V., Artemyeva A.M. Evaluation of the measure of polymorphism information of genetic diversity. Selskokhozyaystvennaya Biologiya = Agricultural Biology. 2015;50(5):571578. (in Rus sian)</mixed-citation></citation-alternatives></ref><ref id="cit3"><label>3</label><citation-alternatives><mixed-citation xml:lang="ru">Dellaporta S.L., Wood J., Hicks J.B. A plant DNA minipreparation: Version II. Plant Mol. Biol. Rep. 1983;1(4):19-21.</mixed-citation><mixed-citation xml:lang="en">Dellaporta S.L., Wood J., Hicks J.B. A plant DNA minipreparation: Version II. Plant Mol. Biol. Rep. 1983;1(4):19-21.</mixed-citation></citation-alternatives></ref><ref id="cit4"><label>4</label><citation-alternatives><mixed-citation xml:lang="ru">Devos K.M., Bryan G.J., Collins A.J., Stephenson P., Gale M.D. Application of two microsatellite sequences in wheat storage proteins as molecular markers. Theor. Appl. Genet. 1995;90:247252.</mixed-citation><mixed-citation xml:lang="en">Devos K.M., Bryan G.J., Collins A.J., Stephenson P., Gale M.D. Application of two microsatellite sequences in wheat storage proteins as molecular markers. Theor. Appl. Genet. 1995;90:247252.</mixed-citation></citation-alternatives></ref><ref id="cit5"><label>5</label><citation-alternatives><mixed-citation xml:lang="ru">Gostimsky S.A., Kokaeva Z.G., Konovalov F.A. Studying plant genome variation using molecular markers. Russian Journal of Genetics. 2005;41(4):378-388. DOI 10.1007/s11177-005-0101-1.</mixed-citation><mixed-citation xml:lang="en">Gostimsky S.A., Kokaeva Z.G., Konovalov F.A. Studying plant genome variation using molecular markers. Russian Journal of Genetics. 2005;41(4):378-388. DOI 10.1007/s11177-005-0101-1.</mixed-citation></citation-alternatives></ref><ref id="cit6"><label>6</label><citation-alternatives><mixed-citation xml:lang="ru">Guyomarc’h H.I., Sourdille P., Charmet G., Edwards J., Bernard M. Characterisation of polymorphic microsatellite markers from Aegi lops tauschii and transferability to the D-genome of bread wheat. Theor. Appl. Genet. 2002;104(6-7):1164-1172.</mixed-citation><mixed-citation xml:lang="en">Guyomarc’h H.I., Sourdille P., Charmet G., Edwards J., Bernard M. Characterisation of polymorphic microsatellite markers from Aegi lops tauschii and transferability to the D-genome of bread wheat. Theor. Appl. Genet. 2002;104(6-7):1164-1172.</mixed-citation></citation-alternatives></ref><ref id="cit7"><label>7</label><citation-alternatives><mixed-citation xml:lang="ru">Khurshut E.E., Adilova A.T., Norbekov J.K., Kushanov F.N., Turaqu lov Kh.S. Molecular analysis of winter wheat varieties in Uzbekistan. Uzb. Biol. J. 2017;(3):44-48.</mixed-citation><mixed-citation xml:lang="en">Khurshut E.E., Adilova A.T., Norbekov J.K., Kushanov F.N., Turaqu lov Kh.S. Molecular analysis of winter wheat varieties in Uzbekistan. Uzb. Biol. J. 2017;(3):44-48.</mixed-citation></citation-alternatives></ref><ref id="cit8"><label>8</label><citation-alternatives><mixed-citation xml:lang="ru">Lowe I., Jankuloski L., Chao S., Chen X., See D., Dubcovsky J. Mapping and validation of QTL which confer partial resistance to broadly virulent post-2000 North American races of stripe rustin hexaploid wheat. Theor. Appl. Genet. 2011;123:143-157.</mixed-citation><mixed-citation xml:lang="en">Lowe I., Jankuloski L., Chao S., Chen X., See D., Dubcovsky J. Mapping and validation of QTL which confer partial resistance to broadly virulent post-2000 North American races of stripe rustin hexaploid wheat. Theor. Appl. Genet. 2011;123:143-157.</mixed-citation></citation-alternatives></ref><ref id="cit9"><label>9</label><citation-alternatives><mixed-citation xml:lang="ru">Nei M., Li W.H. Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc. Natl. Acad. Sci. USA. 1979;76:5269-5273. DOI 10.1073/pnas.76.10.5269.</mixed-citation><mixed-citation xml:lang="en">Nei M., Li W.H. Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc. Natl. Acad. Sci. USA. 1979;76:5269-5273. DOI 10.1073/pnas.76.10.5269.</mixed-citation></citation-alternatives></ref><ref id="cit10"><label>10</label><citation-alternatives><mixed-citation xml:lang="ru">Nei M., Roychoudhury A.K. Sampling variances of heterozygosity and genetic distance. Genetics. 1974;76:379-390.</mixed-citation><mixed-citation xml:lang="en">Nei M., Roychoudhury A.K. Sampling variances of heterozygosity and genetic distance. Genetics. 1974;76:379-390.</mixed-citation></citation-alternatives></ref><ref id="cit11"><label>11</label><citation-alternatives><mixed-citation xml:lang="ru">R Core Team. R: A Language and Environment for Statistical Computing. R Foundation for Statistical Computing. Vienna, Austria, 2016. Available at https://www.R-project.org.</mixed-citation><mixed-citation xml:lang="en">R Core Team. R: A Language and Environment for Statistical Computing. R Foundation for Statistical Computing. Vienna, Austria, 2016. Available at https://www.R-project.org.</mixed-citation></citation-alternatives></ref><ref id="cit12"><label>12</label><citation-alternatives><mixed-citation xml:lang="ru">Röder M.S., Korzun V., Wandehake K., Planschke J., Tixier M.H., Leroy P., Ganal M.W. A microsatellite map of wheat. Genetics. 1998; 149:2007-2023.</mixed-citation><mixed-citation xml:lang="en">Röder M.S., Korzun V., Wandehake K., Planschke J., Tixier M.H., Leroy P., Ganal M.W. A microsatellite map of wheat. Genetics. 1998; 149:2007-2023.</mixed-citation></citation-alternatives></ref><ref id="cit13"><label>13</label><citation-alternatives><mixed-citation xml:lang="ru">Somers D.J., Peter I., Edwards K. A high-density microsatellite consensus map for bread wheat (Triticum aestivum L.). Theor. Appl. Genet. 2004;109:1105-1114.</mixed-citation><mixed-citation xml:lang="en">Somers D.J., Peter I., Edwards K. A high-density microsatellite consensus map for bread wheat (Triticum aestivum L.). Theor. Appl. Genet. 2004;109:1105-1114.</mixed-citation></citation-alternatives></ref><ref id="cit14"><label>14</label><citation-alternatives><mixed-citation xml:lang="ru">Song Q.J., Fickus E.W., Cregan P.B. Characteristics of trinucleotide markers in wheat. Theor. Appl. Genet. 2002;104:286-293.</mixed-citation><mixed-citation xml:lang="en">Song Q.J., Fickus E.W., Cregan P.B. Characteristics of trinucleotide markers in wheat. Theor. Appl. Genet. 2002;104:286-293.</mixed-citation></citation-alternatives></ref><ref id="cit15"><label>15</label><citation-alternatives><mixed-citation xml:lang="ru">Sourdille P., Gandon B., Chiquet V., Nicot N., Somers D., Murigneux A., Bernard M. Wheat génoplante SSR mapping data Figrelease: a new set of markers and comprehensive genetic and physical mapping data. 2004. Available at: http://wheat.pw.usda.gov/ggpages/SSRclub/GeneticPhysical. Accessed on March 10, 2010.</mixed-citation><mixed-citation xml:lang="en">Sourdille P., Gandon B., Chiquet V., Nicot N., Somers D., Murigneux A., Bernard M. Wheat génoplante SSR mapping data Figrelease: a new set of markers and comprehensive genetic and physical mapping data. 2004. Available at: http://wheat.pw.usda.gov/ggpages/SSRclub/GeneticPhysical. Accessed on March 10, 2010.</mixed-citation></citation-alternatives></ref><ref id="cit16"><label>16</label><citation-alternatives><mixed-citation xml:lang="ru">Sulimova G.E. DNA markers in genetic studies: types of markers, their properties, and applications. Uspe khi Sovremennoy Biologii = Advances in Current Biology. 2004;124(3):260-271. (in Russian)</mixed-citation><mixed-citation xml:lang="en">Sulimova G.E. DNA markers in genetic studies: types of markers, their properties, and applications. Uspe khi Sovremennoy Biologii = Advances in Current Biology. 2004;124(3):260-271. (in Russian)</mixed-citation></citation-alternatives></ref><ref id="cit17"><label>17</label><citation-alternatives><mixed-citation xml:lang="ru">Zhao H.X., Liu X.M., Chen M.-S. H22, a major resistance gene to the Hessian fly (Mayetiola destructor), is mapped to the distal region of wheat chromosome 1DS. Theor. Appl. Genet. 2006;113:1491-1496.</mixed-citation><mixed-citation xml:lang="en">Zhao H.X., Liu X.M., Chen M.-S. H22, a major resistance gene to the Hessian fly (Mayetiola destructor), is mapped to the distal region of wheat chromosome 1DS. Theor. Appl. Genet. 2006;113:1491-1496.</mixed-citation></citation-alternatives></ref></ref-list><fn-group><fn fn-type="conflict"><p>The authors declare that there are no conflicts of interest present.</p></fn></fn-group></back></article>
