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<article article-type="research-article" dtd-version="1.3" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:lang="ru"><front><journal-meta><journal-id journal-id-type="publisher-id">vavilov</journal-id><journal-title-group><journal-title xml:lang="ru">Вавиловский журнал генетики и селекции</journal-title><trans-title-group xml:lang="en"><trans-title>Vavilov Journal of Genetics and Breeding</trans-title></trans-title-group></journal-title-group><issn pub-type="epub">2500-3259</issn><publisher><publisher-name>Institute of Cytology and Genetics of Siberian Branch of the RAS</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.18699/VJ18.406</article-id><article-id custom-type="elpub" pub-id-type="custom">vavilov-1648</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="ru"><subject>ГЕНЕТИЧЕСКИЕ РЕСУРСЫ И БИОКОЛЛЕКЦИИ</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="en"><subject>PLANT GENETICS</subject></subj-group></article-categories><title-group><article-title>Анализ полиморфизма генома представителей синтетического вида ×Trititrigia cziczinii Tsvel. методом AFLP</article-title><trans-title-group xml:lang="en"><trans-title>Genome polymorphism  of the synthetic species xTrititrigia cziczinii Tsvel. inferred from AFLP analysis</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0001-9618-5932</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Трифонова</surname><given-names>А. А.</given-names></name><name name-style="western" xml:lang="en"><surname>Trifonova</surname><given-names>A. A.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Москва.</p></bio><bio xml:lang="en"><p>Moscow.</p></bio><email xlink:type="simple">aichka89@mail.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-8479-4949</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Борис</surname><given-names>К. В.</given-names></name><name name-style="western" xml:lang="en"><surname>Boris</surname><given-names>K. V.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Москва.</p></bio><bio xml:lang="en"><p>Moscow.</p></bio><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0003-1531-8938</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Дедова</surname><given-names>Л. В.</given-names></name><name name-style="western" xml:lang="en"><surname>Dedova</surname><given-names>L. V.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Москва.</p></bio><bio xml:lang="en"><p>Moscow.</p></bio><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0001-8677-4354</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Мельник</surname><given-names>В. А.</given-names></name><name name-style="western" xml:lang="en"><surname>Melnik</surname><given-names>V. A.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Москва.</p></bio><bio xml:lang="en"><p>Moscow.</p></bio><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-3466-7263</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Иванова</surname><given-names>Л. П.</given-names></name><name name-style="western" xml:lang="en"><surname>Ivanova</surname><given-names>L. P.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Москва.</p></bio><bio xml:lang="en"><p>Moscow.</p></bio><xref ref-type="aff" rid="aff-2"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-2266-4348</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Кузьмина</surname><given-names>Н. П.</given-names></name><name name-style="western" xml:lang="en"><surname>Kuzmina</surname><given-names>N. P.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Москва.</p></bio><bio xml:lang="en"><p>Moscow.</p></bio><xref ref-type="aff" rid="aff-2"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0001-8264-4499</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Завгородний</surname><given-names>С. В.</given-names></name><name name-style="western" xml:lang="en"><surname>Zavgorodniy</surname><given-names>S. V.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Москва.</p></bio><bio xml:lang="en"><p>Moscow.</p></bio><xref ref-type="aff" rid="aff-2"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-6055-8861</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Упелниек</surname><given-names>В. П.</given-names></name><name name-style="western" xml:lang="en"><surname>Upelniek</surname><given-names>V. P.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Москва.</p></bio><bio xml:lang="en"><p>Moscow.</p></bio><xref ref-type="aff" rid="aff-2"/></contrib></contrib-group><aff-alternatives id="aff-1"><aff xml:lang="ru">Институт общей генетики им. Н.И. Вавилова Российской академии наук.<country>Россия</country></aff><aff xml:lang="en">Vavilov Institute of General Genetics, RAS.<country>Russian Federation</country></aff></aff-alternatives><aff-alternatives id="aff-2"><aff xml:lang="ru">Главный ботанический сад им. Н.В. Цицина Российской академии наук.<country>Россия</country></aff><aff xml:lang="en">N.V. Tsitsin Main Botanical Garden, RAS.<country>Russian Federation</country></aff></aff-alternatives><pub-date pub-type="collection"><year>2018</year></pub-date><pub-date pub-type="epub"><day>25</day><month>09</month><year>2018</year></pub-date><volume>22</volume><issue>6</issue><fpage>648</fpage><lpage>653</lpage><permissions><copyright-statement>Copyright &amp;#x00A9; Трифонова А.А., Борис К.В., Дедова Л.В., Мельник В.А., Иванова Л.П., Кузьмина Н.П., Завгородний С.В., Упелниек В.П., 2018</copyright-statement><copyright-year>2018</copyright-year><copyright-holder xml:lang="ru">Трифонова А.А., Борис К.В., Дедова Л.В., Мельник В.А., Иванова Л.П., Кузьмина Н.П., Завгородний С.В., Упелниек В.П.</copyright-holder><copyright-holder xml:lang="en">Trifonova A.A., Boris K.V., Dedova L.V., Melnik V.A., Ivanova L.P., Kuzmina N.P., Zavgorodniy S.V., Upelniek V.P.</copyright-holder><license license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>This work is licensed under a Creative Commons Attribution 4.0 License.</license-p></license></permissions><self-uri xlink:href="https://vavilov.elpub.ru/jour/article/view/1648">https://vavilov.elpub.ru/jour/article/view/1648</self-uri><abstract><p>×Trititrigia cziczinii Tsvel. – синтетический вид, полученный в результа те гибридизации различных видов пшеницы и пырея. ×T. cziczinii, – обладает уникальными признаками: многолетность, способность к отрастанию после скашивания на зерно, высокая адаптивность, устойчивость к болезням и вредителям, высокое содержание белка и клейковины в зерне. Это позволяет считать его перспективной сельскохозяйственной культурой. Новый вид является удобным  объектом для проведения фундаментальных исследований в области генетики, филогении и эволюции злаков (Poaceae). Однако прежде генетические исследования ×T. cziczinii практически не проводились. Цель настоящей работы – изучение генетиче ского разнообразия 24 представителей двух подвидов (ssp. Submi tans и ssp. Perenne) вида ×T. cziczinii. Для оценки межвидовых раз личий в анализ было включено 17 образцов других видов трибы Triticeae (Triticum aes ti vum, Triticum durum, Agropyron glaucum и Agropyron elongatum, а так же образцы пшенично-пырейных и пшенично-элимусных гибридов (ППГ и ПЭГ)). В работе был применен метод маркирования AFLP (Amplified Fragment Length Polymorphism), который позволил вы явить достаточно высокий уровень полимор физма изученных образцов. С помощью двух комбинаций праймер/фермент (EcoRI-ACT/MseI-CCC и EcoRI-ACT/MseI-CTA) удалось идентифицировать 227 фрагментов, из которых 224 были поли морфны (98.68 %), а уровень внутривидового полиморфизма 24 образцов ×T. cziczinii составил 68.15 %. Найдены фрагменты AFLP-спектров, специфичные для представителей ×T. cziczinii и изу ченных видов пырея, которые могут стать основой для создания маркеров, выявляющих интрогрессии генетического материала рода Agropyron в геноме представителей ×T. cziczinii. Полученные результаты свидетельствуют о большей генетической близости ×T. cziczinii к T. aestivum, чем к представителям рода Agropyron. Так, по данным кластерного анализа, представители ×T. cziczinii и сорта мягкой пшеницы объединились в один подкластер, внутри которого образцы двух видов образуют отдельные группы. При этом в результате оценки внутривидового генетического разнообразия ×T. cziczinii не было выявлено достоверной дифференциации представителей подвидов Submitans и Perenne, что, вероятно, связано с не до конца определенной генетической природой многолетности, основного признака, по которому эти подвиды разделены. Проведенное изучение части уникальной коллекции синтетического вида ×T. cziczinii позволило получить первые данные о  генетике вида, более ранние исследования затрагивали в основном фенотипические и хозяйственно ценные при знаки. Применяемый в на стоящем исследовании метод AFLP-маркиро вания показал высокую эффективность при работе с малоизученным видом, а его результаты перспективны и полезны для понимания генетической структуры нового вида (×T. cziczinii Tsvel.).</p></abstract><trans-abstract xml:lang="en"><p>×Trititrigia cziczinii Tsvel. is a synthetic species obtained as a result of hybridization of different wheat and wheat- grass species. ×T. cziczinii has unique characteristics, as it is a perennial species, with the ability to grow after mowing, high adaptability, resistance to diseases and pests, high protein and gluten content in the grain. All this makes it a promising new crop for agriculture. The new species is a good object for fundamental research in the field of genetics, phylogeny and evolution of cereals (Poaceae). However, there were practically no genetic studies of ×T. cziczinii. The aim of this work was to study the genetic diversity of 24 representatives of two ×T. cziczinii subspecies (ssp. Submitans and ssp. Perenne). To estimate interspecific differences, 17 samples of other tribe Triticeae species (Triticum aestivum, Triticum durum, Agropyron glaucum and Agropyron elon gatum, as well as samples of Triticum-Agropyron and TriticumElymus hybrids) were included in the analysis. For the study, AFLP method (Amplified Fragment Length Polymorphism) was chosen, which allowed us to reveal a sufficiently high polymorphism level of the studied samples. The two primer/enzyme combinations (EcoRI-ACT/MseI-CCC, EcoRI-ACT/MseI-CTA) allow ed the iden tification of 227 fragments, 224 of them were polymorphic (98.68 %), and the level of intraspecific polymorphism of 24 ×T. cziczinii samples was 68.15 %. The iden tified fragments of AFLP spectra, specific for the ×T. cziczinii representatives and the studied wheatgrass species, can be the basis for creating markers that will detect introgressions of genetic material of the genus Agropyron in the T. cziczinii ge nome. Our results indicate a greater genetic relatedness of ×T. cziczinii to T. aestivum than to representatives of the genus Agropyron. According to the cluster analysis, representatives of ×T. cziczinii and varieties of bread wheat were combined into a single subcluster, within which the samples of two species form separate groups. At the same time, the evaluation of the intraspecific genetic diversity of ×T. cziczinii showed  no reliable differentiation of representatives of the subspecies Submitans and Perenne, which is probably due to uncertain genetic nature of perenniality, the main feature that divides these subspecies. The study of the unique ×T. cziczinii collection allowed us to obtain the first data on the genetics of the species, while previous studies were focused mainly on phenotypic and economically valuable traits. AFLP analysis used in this study showed high efficiency when working with less studied species, and its results are promising and useful for understanding the genetic structure of the new species (×T. cziczinii Tsvel.). </p></trans-abstract><kwd-group xml:lang="ru"><kwd>xTrititrigia cziczinii Tsvel.</kwd><kwd>синтетический вид</kwd><kwd>межвидовая гибридизация</kwd><kwd>AFLP-анализ</kwd><kwd>генетическое разнообразие</kwd></kwd-group><kwd-group xml:lang="en"><kwd>xTrititrigia cziczinii Tsvel.</kwd><kwd>synthetic species</kwd><kwd>interspecific hybridization</kwd><kwd>AFLP-analysis</kwd><kwd>genetic diversity</kwd></kwd-group></article-meta></front><back><ref-list><title>References</title><ref id="cit1"><label>1</label><citation-alternatives><mixed-citation xml:lang="ru">Altinkut A., Kazan K., Gozukirmizi N. AFLP marker linked to waterstress­tolerant bulks in barley (Hordeum vulgare L.). Genet. Mol. Biol. 2003;26(1):77­82. 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