vavilovВавиловский журнал генетики и селекцииVavilov Journal of Genetics and Breeding2500-3259Institute of Cytology and Genetics of Siberian Branch of the RAS10.18699/VJ18.411vavilov-1654Research ArticleСелекция растений на иммунитет и продуктивностьPlant breeding for immunity and performanceХарактеристика сортов озимой пшеницы по устойчивости к фузариозу зернаCharacterization of resistance of winter wheat varieties to Fusarium head blighthttps://orcid.org/0000-0002-3276-561XГагкаеваТ. Ю.GagkaevaT. Yu.

Санкт-Петербург, Пушкин.

St. Petersburg, Pushkin.

t.gagkaeva@mail.ruhttps://orcid.org/0000-0002-7657-6618ОринаА. С.OrinaA. S.

Санкт-Петербург, Пушкин.

St. Petersburg, Pushkin.

https://orcid.org/0000-0002-5350-3221ГавриловаО. П.GavrilovaO. P.

Санкт-Петербург, Пушкин.

St. Petersburg, Pushkin.

https://orcid.org/0000-0002-3454-9988АбловаИ. Б.AblovaI. B.

Краснодар.

Krasnodar.

https://orcid.org/0000-0003-3844-9682БеспаловаЛ. А.BespalovaL. A.

Краснодар.

Krasnodar.

Всероссийский научно-исследовательский институт защиты растений.РоссияAll-Russian Institute of Plant Protection.Russian FederationНациональный центр зерна им. П.П. Лукьяненко.РоссияNational Center of Grain named after P.P. Lukyanenko.Russian Federation201826092018226685692Copyright © Гагкаева Т.Ю., Орина А.С., Гаврилова О.П., Аблова И.Б., Беспалова Л.А., 20182018Гагкаева Т.Ю., Орина А.С., Гаврилова О.П., Аблова И.Б., Беспалова Л.А.Gagkaeva T.Y., Orina A.S., Gavrilova O.P., Ablova I.B., Bespalova L.A.This work is licensed under a Creative Commons Attribution 4.0 License.https://vavilov.elpub.ru/jour/article/view/1654

По продуктивности и устойчивости к фузариозу на естественном фоне инфекции и на фоне искусственной инокуляции грибом Fusarium graminearum исследовали 17 сортов озимой пшеницы селекции Национального центра зерна им. П.П. Лукьяненко. Оценку сортов проводили на основании показателей, описывающих различные типы устойчивости: процентное содержание фузариозных зерен, выявленных по внешним признакам и в результате микологического анализа, а также содержание ДНК грибов Fusarium; показатели продуктивности инокулированных растений в сравнении с неинокулированными; количество микотоксинов в зерне. На естественном фоне, согласно результатам микологического анализа, зараженность зерна грибами рода Fusarium была в среднем 6.1 % (0–15 %), количество ДНК F. graminearum варьировало в диапазоне (1.1–42.7) × 10–5 нг/нг ДНК пшеницы, дезоксиниваленол (ДОН) обнаружен в 15 образцах с максимальным содержанием 420 мкг/кг, а фумонизин В1 (ФВ1) не выявлен. На искусственном инфекционном фоне зараженность зерна составила 25.8 % (2–54 %), количество ДНК F. graminearum было значительно выше, чем в естественных условиях, и варьировало в пределах (4.24–49.8) × 10–3 нг/нг. Образцы зерна всех сортов пшеницы, выращенных на искусственном инфекционном фоне F. graminearum, содержали ДОН в высоких количествах – от 20255 до 79245 мкг/кг. Выявлено значительное содержание ФВ1 в диапазоне от 980 до 20326 мкг/кг. Сорт Адель охарактеризован как высокоустойчивый к заражению грибами и накоплению микотоксинов. К относительно устойчивым отнесены сорта Антонина, Лебедь, Память, а наиболее восприимчивым оказался сорт Утриш. Установлены сходство реакций устойчивости сортов пшеницы к заражению F. graminearum и F. verticillioides и существующие между грибами взаимодействия в процессе колонизации зерна.

In this study, naturally and artificially inoculated winter wheat varieties were studied with respect to their productivity and resistance to Fusarium head blight (FHB). We used the following set of disease assessment parameters: the percentage of visually and latent Fusarium-damaged grains (FDG); the DNA content of Fusarium fungi; the productivity of inoculated plants compared with non-inoculated plants; and the amount of mycotoxins in the grain. In case of naturally infected grains, the average FDG was found to be about 6.1 % (range of 0–15 %). The amount of DNA of Fusarium graminearum was found to be in the range of (1.1–42.7) × 10–5 ng/ng wheat DNA. The mycotoxin deoxynivalenol (DON) was detected in 15 samples of grain from plants that were grown under natural infection. The maximum DON amount was found to be 420 μg/kg. Fumonisin B1 (FB1) was not be detected in naturally infected grain. In case of artificially inoculated plants, the average FDG was found to be 25.8 % (2–54 %). The amount of F. graminearum DNA was found to be significantly higher (4.24– 49.8) × 10–3 ng/ng than it was detected in grain of non-inoculated plants. The wheat varieties inoculated with F. graminearum contained DON in high amounts from 20255 to 79245 μg/kg. Furthermore, a significant amount of FB1 was detected in all wheat varieties in the range of 980–20326 μg/kg. Among the analysed wheat varieties, Adel was characterized to be the most resistant to fungal infection as well as to the contamination by mycotoxins. Antonina, Lebed and Pamyat varieties were classified more relatively resistant than that of other varieties, and Utrish variety was found to be the most susceptible to FHB. The similar resistance of wheat varieties against F. graminearum and F. verticillioides infection was recorded, and the interactions between the fungi during the colonization of grain were shown.

пшеницасортаустойчивостьгрибыFusariumДНКмикотоксиныинфекционный фонколичественная ПЦРwheatvarietiesresistancefungiFusariumDNAmycotoxinsartificial inoculationquantitative PCRReferencesAblova I.B., Bes palova L.A., Kolesnikov F.A., Nabokov G.D., Puzyurnaya O.Yu., Filobok V.A. Wheat breeding for disease resistance. Zemledelie = Agriculture. 2014;3:19-22. (in Russian)Ablova I.B., Bes palova L.A., Kolesnikov F.A., Nabokov G.D., Puzyurnaya O.Yu., Filobok V.A. Wheat breeding for disease resistance. Zemledelie = Agriculture. 2014;3:19-22. (in Russian)Ablova I.B., Taranenko S.A. Methodological aspects of the creation of artificial infection background for Fusarium head blight of winter wheat. Evolution of Scientific Technologies in Plant Science. 2004;1:382-390. (in Russian)Ablova I.B., Taranenko S.A. Methodological aspects of the creation of artificial infection background for Fusarium head blight of winter wheat. Evolution of Scientific Technologies in Plant Science. 2004;1:382-390. (in Russian)Alexander N.J., Proctor R.H., McCormick S.P. Genes, gene clusters, and biosynthesis of trichothecenes and fumonisins in Fusarium. Toxin Rev. 2009;28(2-3):198-215. DOI 10.1080/15569540903092142. Audenaert K., Vanheule A., Höfte M., Haesaert G. Deoxynivalenol: a major player in the multifaceted response of Fusarium to its environment. Toxins. 2013;6:1-19. DOI 10.3390/toxins6010001.Alexander N.J., Proctor R.H., McCormick S.P. Genes, gene clusters, and biosynthesis of trichothecenes and fumonisins in Fusarium. Toxin Rev. 2009;28(2-3):198-215. DOI 10.1080/15569540903092142. Audenaert K., Vanheule A., Höfte M., Haesaert G. Deoxynivalenol: a major player in the multifaceted response of Fusarium to its environment. Toxins. 2013;6:1-19. DOI 10.3390/toxins6010001.Babayants L.T., Meshterkhazi А., Vekhter V. Methods of Breeding and Assessment of Disease Resistance of Wheat and Barley in CMEA Countries. Prague, 1988. (in Russian)Babayants L.T., Meshterkhazi А., Vekhter V. Methods of Breeding and Assessment of Disease Resistance of Wheat and Barley in CMEA Countries. Prague, 1988. (in Russian)Bespalova L.A., Romanenko A.A., Ko lesnikov F.A., …, Kalmysh A.P., Ponomarev D.A., Belyakova A.Yu. Varieties of Wheat and Triticale from the Lukyanenko Agricultural Research Institute. Krasnodar, 2017. (in Russian)Bespalova L.A., Romanenko A.A., Ko lesnikov F.A., …, Kalmysh A.P., Ponomarev D.A., Belyakova A.Yu. Varieties of Wheat and Triticale from the Lukyanenko Agricultural Research Institute. Krasnodar, 2017. (in Russian)Bespalova L.A., Vasilyev A.V., Ablova I.B., Filobok V.A., Khudokormova Z.N., Davoyan R.O., Davoyan E.R., Karlov G.I., Solo viev A.A., Divashuk M.G., Mayer N.K., Dudnikov M.V., Mironenko N.V., Baranova O.A. The use of molecular markers in wheat breeding at the Lukyanenko Agricultural Research Institute. Russian Journal of Genetics: Applied Research. 2012;2(4):286-290.]Bespalova L.A., Vasilyev A.V., Ablova I.B., Filobok V.A., Khudokormova Z.N., Davoyan R.O., Davoyan E.R., Karlov G.I., Solo viev A.A., Divashuk M.G., Mayer N.K., Dudnikov M.V., Mironenko N.V., Baranova O.A. The use of molecular markers in wheat breeding at the Lukyanenko Agricultural Research Institute. Russian Journal of Genetics: Applied Research. 2012;2(4):286-290.]Desjardins A.E., Munkvold G.P., Plattner R.D., Proctor R.H. FUM1 – a gene required for fumonisin biosynthesis but not for maize ear rot and ear infection by Gibberella moniliformis in field tests. Mol. Plant-Microbe Interact. 2002;11:1157-1164. DOI 10.1094/MPMI. 2002.15.11.1157.Desjardins A.E., Munkvold G.P., Plattner R.D., Proctor R.H. FUM1 – a gene required for fumonisin biosynthesis but not for maize ear rot and ear infection by Gibberella moniliformis in field tests. Mol. Plant-Microbe Interact. 2002;11:1157-1164. DOI 10.1094/MPMI. 2002.15.11.1157.Desjardins A.E., Plattner R.D. Fumonisin B (1)-nonproducing strains of Fusarium verticillioides cause maize (Zea mays) ear infection and ear rot. J. Agric. Food Chem. 2000;48:5773-5780.Desjardins A.E., Plattner R.D. Fumonisin B (1)-nonproducing strains of Fusarium verticillioides cause maize (Zea mays) ear infection and ear rot. J. Agric. Food Chem. 2000;48:5773-5780.Dill-Macky R., Jones R.K. The effect of previous crop residues and tillage on Fusarium head blight of wheat. Plant Dis. 2000;84:71-76. DOI 10.1094/PDIS.2000.84.1.71.Dill-Macky R., Jones R.K. The effect of previous crop residues and tillage on Fusarium head blight of wheat. Plant Dis. 2000;84:71-76. DOI 10.1094/PDIS.2000.84.1.71.Gagkaeva T.Yu., Gavrilova O.P. Grain infection by Fusarium fungi in the Krasnodar and Stavropol regions. Zashchita i Karantin Rasteniy = Plant Protection and Qua ran tine. 2014;3:30-33. (in Russian)Gagkaeva T.Yu., Gavrilova O.P. Grain infection by Fusarium fungi in the Krasnodar and Stavropol regions. Zashchita i Karantin Rasteniy = Plant Protection and Qua ran tine. 2014;3:30-33. (in Russian)Gerlach W., Nirenberg H. The Genus Fusarium – a Pictorial Atlas Mitt. Biol. Bund. Ld. Berlin, 1982. Halstensen A.S., Nordby K.C., Eduard W., Klemsdal S.S. Real-time PCR detection of toxigenic Fusarium in airborne and settled grain dust and associations with trichothecene mycotoxins. J. Environ. Monit. 2006;8:1235-1241. DOI 10.1039/b609840a.Gerlach W., Nirenberg H. The Genus Fusarium – a Pictorial Atlas Mitt. Biol. Bund. Ld. Berlin, 1982. Halstensen A.S., Nordby K.C., Eduard W., Klemsdal S.S. Real-time PCR detection of toxigenic Fusarium in airborne and settled grain dust and associations with trichothecene mycotoxins. J. Environ. Monit. 2006;8:1235-1241. DOI 10.1039/b609840a.Iglesias J., Presello D.A., Botta G., Lori G.A., Fauguel C.M. Aggressiveness of Fusarium section Liseola isolates causing maize ear rot in Argentina. Eur. J. Plant Pathol. 2010;92(1):205-211. DOI 10.4454/jpp.v92i1.31.Iglesias J., Presello D.A., Botta G., Lori G.A., Fauguel C.M. Aggressiveness of Fusarium section Liseola isolates causing maize ear rot in Argentina. Eur. J. Plant Pathol. 2010;92(1):205-211. DOI 10.4454/jpp.v92i1.31.Maiorano A., Blandino M., Reyneri A., Vanara F. Effects of maize residues on the Fusarium spp. infection and deoxynivalenol (DON) contamination of wheat grain. Crop Prot. 2008;27:182-188. DOI 10.1016/j.cropro.2007.05.004.Maiorano A., Blandino M., Reyneri A., Vanara F. Effects of maize residues on the Fusarium spp. infection and deoxynivalenol (DON) contamination of wheat grain. Crop Prot. 2008;27:182-188. DOI 10.1016/j.cropro.2007.05.004.Mesterhazy A. Theory and practice of the breeding for Fusarium head blight in wheat. J. Appl. Genet. 2002;43A:289-302.Mesterhazy A. Theory and practice of the breeding for Fusarium head blight in wheat. J. Appl. Genet. 2002;43A:289-302.Nicolaisen M., Suproniene S., Nielsen L.K., Lazzaro I., Spliid N.H., Justesen A.F. Real-time PCR for quantification of eleven individual Fusarium species in cereals. J. Microbiol. Methods. 2009;76:234240. DOI 10.1016/j.mimet.2008.10.016.Nicolaisen M., Suproniene S., Nielsen L.K., Lazzaro I., Spliid N.H., Justesen A.F. Real-time PCR for quantification of eleven individual Fusarium species in cereals. J. Microbiol. Methods. 2009;76:234240. DOI 10.1016/j.mimet.2008.10.016.Picot A., Hourcade-Marcolla D., Barreau C., Pinson-Gadais L., Caron D., Richard-Forget F., Lannou C. Interactions between Fusarium verticillioides and Fusarium graminearum in maize ears and consequences for fungal development and mycotoxin accumulation. Plant Pathol. 2012;61:140-151. DOI 10.1111/j.1365-3059.2011.02503.x.Picot A., Hourcade-Marcolla D., Barreau C., Pinson-Gadais L., Caron D., Richard-Forget F., Lannou C. Interactions between Fusarium verticillioides and Fusarium graminearum in maize ears and consequences for fungal development and mycotoxin accumulation. Plant Pathol. 2012;61:140-151. DOI 10.1111/j.1365-3059.2011.02503.x.Preiser V., Goetsch D., Sulyok M., Krska R., Mach R.L., Farnleitner A., Brunner K. The development of a multiplex real-time PCR to quantify Fusarium DNA of trichothecene and fumonisin producing strains in maize. Anal. Methods. 2015;7:1358-1365.Preiser V., Goetsch D., Sulyok M., Krska R., Mach R.L., Farnleitner A., Brunner K. The development of a multiplex real-time PCR to quantify Fusarium DNA of trichothecene and fumonisin producing strains in maize. Anal. Methods. 2015;7:1358-1365.Presello D.A., Iglesias J., Botta G., Lori G.A., Eyherabide G.H. Stability of maize resistance to the ear rots caused by Fusarium graminearum and F. verticillioides in Argentinean and Canadian environments. Euphytica. 2006;147:403-407. DOI 10.1007/s10681-005-9037-8.Presello D.A., Iglesias J., Botta G., Lori G.A., Eyherabide G.H. Stability of maize resistance to the ear rots caused by Fusarium graminearum and F. verticillioides in Argentinean and Canadian environments. Euphytica. 2006;147:403-407. DOI 10.1007/s10681-005-9037-8.Stanković S., Lević J., Krnjaja V. Fumonisin B1 in maize, wheat and barley grain in Serbia. Biotechnol. Animal Husb. 2011;27(3):631641. DOI 10.2298/BAH1103631S.Stanković S., Lević J., Krnjaja V. Fumonisin B1 in maize, wheat and barley grain in Serbia. Biotechnol. Animal Husb. 2011;27(3):631641. DOI 10.2298/BAH1103631S.Yli-Mattila T., Paavanen-Huhtala S., Jestoi M., Parikka P., Hietaniemi V., Gagkaeva T., Sarlin T., Haikara A., Laaksonen S., Rizzo A. Real-time PCR detection and quantification of Fusarium poae, F. gra minearum, F. sporotrichioides and F. langsethiae in cereal grains in Finland and Russia. Arch. Phytopathol. Plant Protect. 2008; 41:243-260. DOI 10.1080/03235400600680659.Yli-Mattila T., Paavanen-Huhtala S., Jestoi M., Parikka P., Hietaniemi V., Gagkaeva T., Sarlin T., Haikara A., Laaksonen S., Rizzo A. Real-time PCR detection and quantification of Fusarium poae, F. gra minearum, F. sporotrichioides and F. langsethiae in cereal grains in Finland and Russia. Arch. Phytopathol. Plant Protect. 2008; 41:243-260. DOI 10.1080/03235400600680659.

The authors declare that there are no conflicts of interest present.