vavilovВавиловский журнал генетики и селекцииVavilov Journal of Genetics and Breeding2500-3259Institute of Cytology and Genetics of Siberian Branch of the RAS10.18699/VJ18.412vavilov-1655Research ArticleСелекция растений на иммунитет и продуктивностьPlant breeding for immunity and performanceПоследовательности, гомологичные участкам гена RB/Rpi-blb1/Rpi-sto1, у сортов картофеля, созданных методами традиционной селекцииFinding RB/Rpi-blb1/Rpi-sto1-like sequences in conventionally bred potato varietieshttps://orcid.org/0000-0001-8334-8069АнтоноваО. Ю.AntonovaO. Y.

Санкт-Петербург.

St. Petersburg.

https://orcid.org/0000-0002-5432-6466КлименкоН. С.KlimenkoN. S.

Санкт-Петербург.

St. Petersburg.

https://orcid.org/0000-0001-8413-1769 ЕвдокимоваЗ. З.EvdokimovaZ. Z.

Ленинградская область, Гатчинский район.

Leningrad region, Gatchinsky district.

https://orcid.org/0000-0002-6413-9189КостинаЛ. И.KostinaL. I.

Санкт-Петербург.

St. Petersburg.

https://orcid.org/0000-0002-2605-6569 ГавриленкоТ. А.GavrilenkoT. A.

Санкт-Петербург.

St. Petersburg.

tatjana9972@yandex.ruФедеральный исследовательский центр Всероссийский институт генетических ресурсов растений им. Н.И. Вавилова (ВИР).РоссияFederal Research Center the N.I. Vavilov All-Russian Institute of Plant Genetic Resources (VIR).Russian FederationЛенинградский научно-исследовательский институт сельского хозяйства «Белогорка».РоссияLeningrad Scientific Research Institute “Belogorka”.Russian FederationФедеральный исследовательский центр Всероссийский институт генетических ресурсов растений им. Н.И. Вавилова (ВИР); Санкт-Петербургский государственный университет, биологический факультет.РоссияFederal Research Center the N.I. Vavilov All-Russian Institute of Plant Genetic Resources (VIR); St. Petersburg State University, Biological faculty.Russian Federation201826092018226693702Copyright © Антонова О.Ю., Клименко Н.С.,  Евдокимова З.З., Костина Л.И.,  Гавриленко Т.А., 20182018Антонова О.Ю., Клименко Н.С.,  Евдокимова З.З., Костина Л.И.,  Гавриленко Т.А.Antonova O.Y., Klimenko N.S., Evdokimova Z.Z., Kostina L.I., Gavrilenko T.A.This work is licensed under a Creative Commons Attribution 4.0 License.https://vavilov.elpub.ru/jour/article/view/1655

Традиционные задачи селекции картофеля – повышение урожайности и устойчивости к многочисленным патогенам и вредителям. Из них наибольший ущерб картофелеводству как в России, так и мире наносит фитофтороз, вызывае- мый оомицетом Phytophthora infestans. Дикие виды картофеля используются в селекции в качестве источников R ге- нов устойчивости к патогенам. Особый интерес представляют мексиканские виды, поскольку Мексика – центр происхождения и разнообразия P. infestans и центр разнообразия видов картофеля. Дикие мексиканские виды картофеля S. bulbocastanum и S. stoloniferum являются источниками R генов устойчивости к широкому спектру рас P. infestans  (Rpi-blb1, Rpi-blb2, Rpi-sto1). В последние годы эти гены были интрогрессированы в геном культурного картофеля с использованием методов цис-генетики. В то же время высока вероятность выявления генотипов с геном Rpi-sto1 (функциональный гомолог Rpi-blb1) у сортов, созданных методами традиционной селекции, поскольку уже около 40 лет селекционеры используют S. stoloniferum в качестве источника устойчивости к наиболее вредоносному вирусу картофеля – PVY. В настоящей работе проведен молекулярный скрининг 188 сортов картофеля российской селекции и стран ближнего зарубежья с ген-специфичными маркерами RB/Rpi-blb1, Rpi-sto1 и Rpi-blb2; маркерами, сцепленными с генами Rysto, Ry-fsto, детерминирующими устой чивость к PVY, и маркером митотипа gamma, ассоциированного с мужской стерильностью S. stoloniferum гибридов. Отобранные в молекулярном скрининге генотипы могут быть разделены на четыре группы: (А) 13 устойчивых к PVY сортов с диагностическими маркерами генов Rysto, Ry-fsto и со стерильным мт-типом gamma; (В) четыре сорта с мт-ти пом gamma, не обладающие маркерами R генов устойчивости, интрогрессированых от S. stoloniferum; (С) восемь генотипов, у которых были детектированы все пять ген-специфич ных маркеров гена RB/Rpi-blb1/Rpi-sto1; (D) оставшиеся 166 (86.9 %) сортов выборки, у которых не были выявлены маркеры R генов устойчивости S. stoloniferum и митотип gamma. Последовательности ПЦР продуктов, полученные при амплификации с ген-специфичными праймерами Rpi-sto1 и BLBF/R, у всех генотипов группы С были идентичны и имели 99 и 100 % сходства с соответствующими фрагментами референсных последовательностей генов Rpi-sto1 и Rpi-blb1 из GenBank. В группе С выявлены генотипы с различными мт-типами, среди них – образцы с мужской фертильностью.

The main objectives in potato breeding are increasing yield abilities and improving resistance to numerous pathogens and pests. Among them, the late blight caused by the Phytophthora infestans oomycete is one of the most destructive potato diseases both in Russia and worldwide. Wild relatives of cultivated potato are traditionally used in breeding as the source of valuable R genes conferring resistance to pathogens. Of particular interest are Mexican wild species because Mexico is the centre of origin and diversity of P. infestans and at the same time, it is the centre of potato species diversity.  Mexican wild potato species S. bulbocastanum and S. stoloniferum are an important source of the R genes conferring broad-spec trum resistance against various isolates of P. infestans (Rpi-blb1,  Rpi-blb2, Rpi-sto1). Recently these genes have been transferred into cultivated potato gene pool using the cisgene  approach. At the same time there is a high probability of finding geno types with the Rpi-sto1 gene (functional homologues of  Rpi-blb1) among conventionally bred varieties because for about 40 years S. stoloniferum has been used in breeding as a source of the Rysto and Ry-fsto genes of the extreme resistance to the most important viral pathogen PVY. In this study 188 potato varieties bred in Russia and in near-abroad countries were screened for the presence of six gene-specific markers of the RB/Rpi-blb1 =  Rpi-sto1 and Rpi-blb2 genes conferring broad-spectrum resistance against P. infestans, and for the markers linked to the Rysto and Ry-fsto genes conferring extreme resistance to PVY. In addition, a marker for detecting male sterile mitochondrial DNA type gamma derived from S. stoloniferum was used. The genotypes selected through the molecular markers were divided into four groups: (A) 13 PVY resistant varieties carrying diagnostic markers of the Rysto, Ry-fsto genes and having sterile mt-type gamma; (B) four varieties possessing mt-type gamma and not having the markers of the R genes introgressed from S. stoloniferum; (C) eight genotypes carrying five gene-specific markers for the RB/Rpi-blb1/= Rpi-sto1; (D) the rest 166 (86.9 %) varieties not possessing any of the diagnostic markers associated with the S. stoloniferum genetic material. The sequences of the Rpi-sto1- and BLB1 F/R-amplicons were identical in all the genotypes of group ‘C’ and showed respective 99 % and 100 % similarity to the corresponding fragments of the Rpi-sto1 and Rpi-blb1 genes from the GenBank database. Among the genotypes of group ‘C’ various mt-types were detected, and some of them were male fertile.

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The authors declare that there are no conflicts of interest present.