<?xml version="1.0" encoding="UTF-8"?>
<!DOCTYPE article PUBLIC "-//NLM//DTD JATS (Z39.96) Journal Publishing DTD v1.3 20210610//EN" "JATS-journalpublishing1-3.dtd">
<article article-type="research-article" dtd-version="1.3" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:lang="ru"><front><journal-meta><journal-id journal-id-type="publisher-id">vavilov</journal-id><journal-title-group><journal-title xml:lang="ru">Вавиловский журнал генетики и селекции</journal-title><trans-title-group xml:lang="en"><trans-title>Vavilov Journal of Genetics and Breeding</trans-title></trans-title-group></journal-title-group><issn pub-type="epub">2500-3259</issn><publisher><publisher-name>Institute of Cytology and Genetics of Siberian Branch of the RAS</publisher-name></publisher></journal-meta><article-meta><article-id custom-type="elpub" pub-id-type="custom">vavilov-170</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="ru"><subject>Статьи</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="en"><subject>Articles</subject></subj-group></article-categories><title-group><article-title>ФИЛОГЕНЕТИЧЕСКИЕ ОТНОШЕНИЯ НЕКОТОРЫХ ВИДОВ РОДА AQUILEGIA СЕВЕРНОЙ АЗИИ ПО РАЗЛИЧНЫМ ДНК-МАРКЕРАМ</article-title><trans-title-group xml:lang="en"><trans-title>PHYLOGENETIC RELATIONSHIPS AMONG SEVERAL NORTH-ASIAN SPECIES OF THE GENUS AQUILEGIA ACCORDING TO VARIOUS MOLECULAR MARKERS</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Эрст</surname><given-names>А. С.</given-names></name><name name-style="western" xml:lang="en"><surname>Erst</surname><given-names>A. S.</given-names></name></name-alternatives><email xlink:type="simple">erst_andrew@yahoo.com</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Ваулин</surname><given-names>О. В.</given-names></name><name name-style="western" xml:lang="en"><surname>Vaulin</surname><given-names>O. V.</given-names></name></name-alternatives><email xlink:type="simple">oleg.v.vaulin@mail.ru</email><xref ref-type="aff" rid="aff-2"/></contrib></contrib-group><aff-alternatives id="aff-1"><aff xml:lang="ru">Федеральное государственное бюджетное учреждение науки Центральный сибирский ботанический сад Сибирского отделения&#13;
Российской академии наук, Новосибирск, Россия<country>Россия</country></aff><aff xml:lang="en">Central Siberian Botanical Garden SB RAS, Novosibirsk, Russia<country>Russian Federation</country></aff></aff-alternatives><aff-alternatives id="aff-2"><aff xml:lang="ru">Федеральное государственное бюджетное научное учреждение «Федеральный исследовательский центр Институт цитологии и генетики Сибирского отделения Российской академии наук», Новосибирск, Россия<country>Россия</country></aff><aff xml:lang="en">Institute of Cytology and Genetics SB RAS, Novosibirsk, Russia<country>Russian Federation</country></aff></aff-alternatives><pub-date pub-type="collection"><year>2013</year></pub-date><pub-date pub-type="epub"><day>01</day><month>01</month><year>2015</year></pub-date><volume>17</volume><issue>3</issue><fpage>477</fpage><lpage>486</lpage><permissions><copyright-statement>Copyright &amp;#x00A9; Эрст А.С., Ваулин О.В., 2015</copyright-statement><copyright-year>2015</copyright-year><copyright-holder xml:lang="ru">Эрст А.С., Ваулин О.В.</copyright-holder><copyright-holder xml:lang="en">Erst A.S., Vaulin O.V.</copyright-holder><license license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>This work is licensed under a Creative Commons Attribution 4.0 License.</license-p></license></permissions><self-uri xlink:href="https://vavilov.elpub.ru/jour/article/view/170">https://vavilov.elpub.ru/jour/article/view/170</self-uri><abstract><p>Результаты молекулярно-генетического исследования нуклеотидной последовательности межгенного участка rpl32-trnL и фрагмента генов рРНК ITS1-ITS2 8 видов рода водосбор (Aquilegia) из Северной Азии показали генетическую неоднородность популяций A. borodinii и A. jucunda из разных точек ареала. Молекулярно-генетические данные подтверждают обособленность A. viridiflora и A. atropurpurea от остальных изученных видов. Оценена степень родства A. aradanica, A. sibirica и A. synakensis. Доказано гибридогенное происхождение вида A. jucunda.</p></abstract><trans-abstract xml:lang="en"><p>Molecular study of the nucleotide sequence of the rpl32-trnL spacer and the ITS1-ITS2 fragment of rRNA genes in eight species of the genus Aquilegia sampled in Northern Asia has shown that populations of A. borodinii and A. jucunda from various localities are genetically diverse. The results confi rm that A. viridifl ora and A. atropurpurea are genetically distant from other species studied. The relationship degrees among A. aradanica, A. sibirica, and A. synakensis have been determined. The hybrid origin of A. jucunda is proven.</p></trans-abstract><kwd-group xml:lang="ru"><kwd>Aquilegia</kwd><kwd>филогения</kwd><kwd>виды</kwd><kwd>популяции</kwd><kwd>молекулярные маркеры</kwd><kwd>rpl32-trnL</kwd><kwd>ITS1-ITS2</kwd></kwd-group><kwd-group xml:lang="en"><kwd>Aquilegia</kwd><kwd>phylogeny</kwd><kwd>species</kwd><kwd>populations</kwd><kwd>molecular markers</kwd><kwd>rpl32-trnL</kwd><kwd>ITS1-ITS2</kwd></kwd-group><funding-group xml:lang="ru"><funding-statement>гранты РФФИ (№ 12-04-31209 мол_а, 13-04-90848 мол-рф-нр 13-04-00874а и 12-04-01319а)</funding-statement></funding-group></article-meta></front><back><ref-list><title>References</title><ref id="cit1"><label>1</label><citation-alternatives><mixed-citation xml:lang="ru">Васильева И.М. Aquilegia tuvinica Vassil. // Ботан. журнал. 1992. Т. 77. № 9. С. 67.</mixed-citation><mixed-citation xml:lang="en">Васильева И.М. Aquilegia tuvinica Vassil. // Ботан. журнал. 1992. Т. 77. № 9. С. 67.</mixed-citation></citation-alternatives></ref><ref id="cit2"><label>2</label><citation-alternatives><mixed-citation xml:lang="ru">Васильева И.М. Система рода Aquilegia L. (Ranunculaceae) Флоры России и сопредельных государств // Новости систематики высших растений. 1996. Т. 30. С. 8–29.</mixed-citation><mixed-citation xml:lang="en">Васильева И.М. Система рода Aquilegia L. (Ranunculaceae) Флоры России и сопредельных государств // Новости систематики высших растений. 1996. Т. 30. С. 8–29.</mixed-citation></citation-alternatives></ref><ref id="cit3"><label>3</label><citation-alternatives><mixed-citation xml:lang="ru">Губанов И.А. Камелин Р.В. Aquilegia gubanovii R. Kam. // Бюл. МОИП. Отд. биол. 1991. Т. 96. Вып. 6. С. 114.</mixed-citation><mixed-citation xml:lang="en">Губанов И.А. Камелин Р.В. Aquilegia gubanovii R. Kam. // Бюл. МОИП. Отд. биол. 1991. Т. 96. Вып. 6. С. 114.</mixed-citation></citation-alternatives></ref><ref id="cit4"><label>4</label><citation-alternatives><mixed-citation xml:lang="ru">Камелин Р.В. Флорогенетический анализ естественной флоры горной Средней Азии. Л.: Наука, 1973. С. 301–305.</mixed-citation><mixed-citation xml:lang="en">Камелин Р.В. Флорогенетический анализ естественной флоры горной Средней Азии. Л.: Наука, 1973. С. 301–305.</mixed-citation></citation-alternatives></ref><ref id="cit5"><label>5</label><citation-alternatives><mixed-citation xml:lang="ru">Луферов А.Н. Aquilegia L. // Сосудистые растения Советского Дальнего Востока. 1995. Т. 7. С. 26–32.</mixed-citation><mixed-citation xml:lang="en">Луферов А.Н. Aquilegia L. // Сосудистые растения Советского Дальнего Востока. 1995. Т. 7. С. 26–32.</mixed-citation></citation-alternatives></ref><ref id="cit6"><label>6</label><citation-alternatives><mixed-citation xml:lang="ru">Пешкова Г.А. Aquilegia buriatica Peschkova // Новости систематики высших растений. Л., 1977. Т. 14. С. 236.</mixed-citation><mixed-citation xml:lang="en">Пешкова Г.А. Aquilegia buriatica Peschkova // Новости систематики высших растений. Л., 1977. Т. 14. С. 236.</mixed-citation></citation-alternatives></ref><ref id="cit7"><label>7</label><citation-alternatives><mixed-citation xml:lang="ru">Фризен Н.В. Aquilegia L. // Флора Сибири. Новосибирск: Наука, 1993. Т. 6. С. 112–116.</mixed-citation><mixed-citation xml:lang="en">Фризен Н.В. Aquilegia L. // Флора Сибири. Новосибирск: Наука, 1993. Т. 6. С. 112–116.</mixed-citation></citation-alternatives></ref><ref id="cit8"><label>8</label><citation-alternatives><mixed-citation xml:lang="ru">Фризен Н.В. Ультраскульптура поверхности семян и систематика сибирских представителей рода Aquilegia L. (Ranunculaceae) // Бюл. МОИП. Отд. биол. 1989. Т. 94. № 6. С. 76–78.</mixed-citation><mixed-citation xml:lang="en">Фризен Н.В. Ультраскульптура поверхности семян и систематика сибирских представителей рода Aquilegia L. (Ranunculaceae) // Бюл. МОИП. Отд. биол. 1989. Т. 94. № 6. С. 76–78.</mixed-citation></citation-alternatives></ref><ref id="cit9"><label>9</label><citation-alternatives><mixed-citation xml:lang="ru">Шауло Д.Н., Эрст А.С. Новый вид рода Водосбор (Aquilegia L., Ranunculaceae) из Тувы // Turczaninowia. 2010. Т. 13. № 3. С. 43–45.</mixed-citation><mixed-citation xml:lang="en">Шауло Д.Н., Эрст А.С. Новый вид рода Водосбор (Aquilegia L., Ranunculaceae) из Тувы // Turczaninowia. 2010. Т. 13. № 3. С. 43–45.</mixed-citation></citation-alternatives></ref><ref id="cit10"><label>10</label><citation-alternatives><mixed-citation xml:lang="ru">Шауло Д.Н., Эрст А.С. Новый вид Aquilegia (Ranunculaceae) из Западного Саяна, Северная Азия // Turczaninowia. 2011. Т. 14. № 3. С. 28–34.</mixed-citation><mixed-citation xml:lang="en">Шауло Д.Н., Эрст А.С. Новый вид Aquilegia (Ranunculaceae) из Западного Саяна, Северная Азия // Turczaninowia. 2011. Т. 14. № 3. С. 28–34.</mixed-citation></citation-alternatives></ref><ref id="cit11"><label>11</label><citation-alternatives><mixed-citation xml:lang="ru">Bastida J., Alcantara J., Rey P. et al. Extended phylogeny of Aquilegia: the biogeographical and ecological patterns of two simultaneous but contrasting radiations // Plant Syst. Evol. 2010. V. 284. P. 171–185.</mixed-citation><mixed-citation xml:lang="en">Bastida J., Alcantara J., Rey P. et al. Extended phylogeny of Aquilegia: the biogeographical and ecological patterns of two simultaneous but contrasting radiations // Plant Syst. Evol. 2010. V. 284. P. 171–185.</mixed-citation></citation-alternatives></ref><ref id="cit12"><label>12</label><citation-alternatives><mixed-citation xml:lang="ru">Bender W., Spierer P., Hognes D.S., Chambon P. Chromosomal walking and jumping to isolate DNA from Ace and rosy loci of bithorax loci in Drosophila melanogaster // J. Mol. Biol. 1983. V. 168. No 1. P. 17–33.</mixed-citation><mixed-citation xml:lang="en">Bender W., Spierer P., Hognes D.S., Chambon P. Chromosomal walking and jumping to isolate DNA from Ace and rosy loci of bithorax loci in Drosophila melanogaster // J. Mol. Biol. 1983. V. 168. No 1. P. 17–33.</mixed-citation></citation-alternatives></ref><ref id="cit13"><label>13</label><citation-alternatives><mixed-citation xml:lang="ru">Fior S., Li. M., Oxelman B. et al. Spatiotemporal reconstruction of the Aquilegia rapid radiation through next-generation sequencing of rapidly evolving cpDNA regions // New Phytol. 2013. V. 198. No 2. P. 579–592.</mixed-citation><mixed-citation xml:lang="en">Fior S., Li. M., Oxelman B. et al. Spatiotemporal reconstruction of the Aquilegia rapid radiation through next-generation sequencing of rapidly evolving cpDNA regions // New Phytol. 2013. V. 198. No 2. P. 579–592.</mixed-citation></citation-alternatives></ref><ref id="cit14"><label>14</label><citation-alternatives><mixed-citation xml:lang="ru">Fraser A.C. Studies on the Genus Aquilegia. N.Y., 1918. 98 p. Higgins D.G, Thompson J.D, Gibson T.J. Using CLUSTAL for multiple sequence alignments // Methods Enzymol. 1996. V. 266. P. 383–402.</mixed-citation><mixed-citation xml:lang="en">Fraser A.C. Studies on the Genus Aquilegia. N.Y., 1918. 98 p. Higgins D.G, Thompson J.D, Gibson T.J. Using CLUSTAL for multiple sequence alignments // Methods Enzymol. 1996. V. 266. P. 383–402.</mixed-citation></citation-alternatives></ref><ref id="cit15"><label>15</label><citation-alternatives><mixed-citation xml:lang="ru">Kimura M. A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences // J. Mol. Evol. 1980. V. 16. P. 111–120.</mixed-citation><mixed-citation xml:lang="en">Kimura M. A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences // J. Mol. Evol. 1980. V. 16. P. 111–120.</mixed-citation></citation-alternatives></ref><ref id="cit16"><label>16</label><citation-alternatives><mixed-citation xml:lang="ru">Kramer E.M. Aquilegia: a new model for plant development, ecology, and evolution // Annu. Rev. Plant Biol. 2009. V. 60. P. 261–277.</mixed-citation><mixed-citation xml:lang="en">Kramer E.M. Aquilegia: a new model for plant development, ecology, and evolution // Annu. Rev. Plant Biol. 2009. V. 60. P. 261–277.</mixed-citation></citation-alternatives></ref><ref id="cit17"><label>17</label><citation-alternatives><mixed-citation xml:lang="ru">Kramer E.M, Hodges S.A. Aquilegia as a model system for the evolution and ecology of petals // Philos. Trans. Roy. Soc. Lond. Ser. B. 2010. V. 365. P. 477–490.</mixed-citation><mixed-citation xml:lang="en">Kramer E.M, Hodges S.A. Aquilegia as a model system for the evolution and ecology of petals // Philos. Trans. Roy. Soc. Lond. Ser. B. 2010. V. 365. P. 477–490.</mixed-citation></citation-alternatives></ref><ref id="cit18"><label>18</label><citation-alternatives><mixed-citation xml:lang="ru">Munz P.A. Aquilegia: The cultivated and wild columbines // Gentes Herb. 1946. V. 7. P. 1–150.</mixed-citation><mixed-citation xml:lang="en">Munz P.A. Aquilegia: The cultivated and wild columbines // Gentes Herb. 1946. V. 7. P. 1–150.</mixed-citation></citation-alternatives></ref><ref id="cit19"><label>19</label><citation-alternatives><mixed-citation xml:lang="ru">Nold R. Columbines: Aquilegia, Paraquilegia and Semia quilegia. Portland, Timber Press, 2003. 192 p.</mixed-citation><mixed-citation xml:lang="en">Nold R. Columbines: Aquilegia, Paraquilegia and Semia quilegia. Portland, Timber Press, 2003. 192 p.</mixed-citation></citation-alternatives></ref><ref id="cit20"><label>20</label><citation-alternatives><mixed-citation xml:lang="ru">Pallas P. Aquilegia viridifl ora Pall. // Acta Acad. Scientiarum Imperialis Petropolitanae. 1779. V. 2. P. 260. pl. 11, f. 1.</mixed-citation><mixed-citation xml:lang="en">Pallas P. Aquilegia viridifl ora Pall. // Acta Acad. Scientiarum Imperialis Petropolitanae. 1779. V. 2. P. 260. pl. 11, f. 1.</mixed-citation></citation-alternatives></ref><ref id="cit21"><label>21</label><citation-alternatives><mixed-citation xml:lang="ru">Prazmo W. Cytogenetic studies on the genus Aquilegia. III. Inheritance of the traits distinguishing different complexes in the genus // Acta Soc. Bot. 1965. V. 34. P. 403–437.</mixed-citation><mixed-citation xml:lang="en">Prazmo W. Cytogenetic studies on the genus Aquilegia. III. Inheritance of the traits distinguishing different complexes in the genus // Acta Soc. Bot. 1965. V. 34. P. 403–437.</mixed-citation></citation-alternatives></ref><ref id="cit22"><label>22</label><citation-alternatives><mixed-citation xml:lang="ru">Ro K.-E., McPheron B. Molecular phylogeny of the Aquilegia group (Ranunculaceae) based on internal transcribed spacers and 5.8S nuclear ribosomal DNA // Biochem. Syst. Ecol. 1997. V. 25. P. 445–461.</mixed-citation><mixed-citation xml:lang="en">Ro K.-E., McPheron B. Molecular phylogeny of the Aquilegia group (Ranunculaceae) based on internal transcribed spacers and 5.8S nuclear ribosomal DNA // Biochem. Syst. Ecol. 1997. V. 25. P. 445–461.</mixed-citation></citation-alternatives></ref><ref id="cit23"><label>23</label><citation-alternatives><mixed-citation xml:lang="ru">Shaulo D., Erst A. New taxa and taxonomic combination of the genus Aquilegia from Asia // Phytokeys. 2013. In press.</mixed-citation><mixed-citation xml:lang="en">Shaulo D., Erst A. New taxa and taxonomic combination of the genus Aquilegia from Asia // Phytokeys. 2013. In press.</mixed-citation></citation-alternatives></ref><ref id="cit24"><label>24</label><citation-alternatives><mixed-citation xml:lang="ru">Shaw J., Lickey E.B, Schilling E.E., Small R.L. Comparison of whole chloroplast genome sequences to choose noncoding regions for phylogenetic studies in Angiosperms: the tortoise and the hare III // Amer. J. Bot. 2007. V. 94. No. 3. P. 275–288.</mixed-citation><mixed-citation xml:lang="en">Shaw J., Lickey E.B, Schilling E.E., Small R.L. Comparison of whole chloroplast genome sequences to choose noncoding regions for phylogenetic studies in Angiosperms: the tortoise and the hare III // Amer. J. Bot. 2007. V. 94. No. 3. P. 275–288.</mixed-citation></citation-alternatives></ref><ref id="cit25"><label>25</label><citation-alternatives><mixed-citation xml:lang="ru">Tamura K., Peterson D., Peterson N. et al. MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods // Mol. Biol. Evol. 2011. V. 28. No. 10. P. 2731–2739.</mixed-citation><mixed-citation xml:lang="en">Tamura K., Peterson D., Peterson N. et al. MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods // Mol. Biol. Evol. 2011. V. 28. No. 10. P. 2731–2739.</mixed-citation></citation-alternatives></ref><ref id="cit26"><label>26</label><citation-alternatives><mixed-citation xml:lang="ru">Taylor R.J. Interspecific hybridization and its evolutionary significance in the genus Aquilegia // Brittonia. 1967. V. 19. P. 374–390.</mixed-citation><mixed-citation xml:lang="en">Taylor R.J. Interspecific hybridization and its evolutionary significance in the genus Aquilegia // Brittonia. 1967. V. 19. P. 374–390.</mixed-citation></citation-alternatives></ref><ref id="cit27"><label>27</label><citation-alternatives><mixed-citation xml:lang="ru">Willdenow C. Aquilegia atropurpurea Willd. // Enumeratio Plantarum Horti Botanici Berolinensis. 1809. P. 577.</mixed-citation><mixed-citation xml:lang="en">Willdenow C. Aquilegia atropurpurea Willd. // Enumeratio Plantarum Horti Botanici Berolinensis. 1809. P. 577.</mixed-citation></citation-alternatives></ref></ref-list><fn-group><fn fn-type="conflict"><p>The authors declare that there are no conflicts of interest present.</p></fn></fn-group></back></article>
