vavilovВавиловский журнал генетики и селекцииVavilov Journal of Genetics and Breeding2500-3259Institute of Cytology and Genetics of Siberian Branch of the RAS10.18699/VJ18.423vavilov-1713Research ArticleГЕНОФОНД И СЕЛЕКЦИЯ РАСТЕНИЙPLANT GENE POOL AND BREEDINGАнализ генетической коллекции земляники (Fragaria L.) по генам Rca2 и Rpfl с использованием молекулярных маркеровAnalysis of strawberry genetic collection (Fragaria L.) for Rca2 and Rpfl genes with molecular markershttps://orcid.org/0000-0003-1626-840XЛукъянчукИ. В.Luk’yanchukI. V.

Мичуринск, Тамбовская область

Michurinsk, Tambov region

irina.lk2011@yandex.ruhttps://orcid.org/0000-0001-9770-8731ЛыжинА. С.LyzhinA. S.

Мичуринск, Тамбовская область

Michurinsk, Tambov region

https://orcid.org/0000-0003-4150-3037КозловаИ. И.KozlovaI. I.

Мичуринск, Тамбовская область

Michurinsk, Tambov region

Федеральный научный центр им. И.В. МичуринаРоссияI.V. Michurin Federal Scientific CentreRussian Federation201808112018227795799Copyright © Лукъянчук И.В., Лыжин А.С., Козлова И.И., 20182018Лукъянчук И.В., Лыжин А.С., Козлова И.И.Luk’yanchuk I.V., Lyzhin A.S., Kozlova I.I.This work is licensed under a Creative Commons Attribution 4.0 License.https://vavilov.elpub.ru/jour/article/view/1713

Сорта земляники садовой (Fragariax ananassa Duch.) восприимчивы ко многим грибным заболеваниям. Идентификация форм, несущих гены устойчивости, является важным этапом селекционных программ по созданию устойчивых сортов. Использование молекулярных маркеров позволяет с высокой надежностью определить присутствие в геноме необходимых генов и идентифицировать перспективные формы. К числу распространенных заболеваний земляники, наносящих значительный ущерб насаждениям, относятся антракнозная гниль (Colletotrichum acutatum Simmonds) и фитофторозное увядание (Phytophthora fragariae var. fragariae Hickman). Моногенная устойчивость к C. acutatum второй группы патогенности контролируется доминантным геном Rca2. Моногенная устойчивость земляники к фитофторозной корневой гнили детерминирована несколькими олигогенами - Rpf1, Rpf2, Rpf3. Целью настоящего исследования было молекулярно-генетическое тестирование генотипов рода Fragaria L. для идентификации носителей аллелей Rca2 устойчивости к антракнозу и Rpf1 устойчивости к фитофторозной корневой гнили. Объектами исследования являлись дикорастущие виды рода Fragaria L. и сорта земляники ананасной (Fragariax ananassa Duch.) различного эколого-географического происхождения. Для оценки аллельного состояния гена Rca2 устойчивости к антракнозу использовали доминантный SCAR-маркер STS-Rca2_240, локализованный на расстоянии 2.8 cM от гена. для выявления гена Rpf1 устойчивости к фи-тофторозной корневой гнили использовали доминантный SCAR-маркер R1A, находящийся на расстоянии 3.0 cM от гена. Доминантный аллель маркера STS-Rca2_240 идентифицирован у сорта Laetitia (генотип Rca2Rca2 или Rca2rca2), что позволяет рекомендовать его в качестве перспективного источника устойчивости к антракнозу для селекции. Остальные изученные формы характеризуются рецессивным гомозиготным состоянием маркера STS-Rca2_240 (предполагаемый генотип rca2rca2). Доминантный аллель маркера SCAR-R1A у изучаемых сортов и дикорастущих видов земляники не выявлен, что предположительно свидетельствует об их рецессивном гомозиготном генотипе по гену Rpf1 (rpf1rpf1 ).

Strawberry (Fragaria x ananassa Duch.) varieties are susceptible to many fungal diseases. Identification of forms, carrying resistance genes, is an important stage in breeding programs leading to resistant varieties. The use of molecular markers allows to determine with high reliability the presence of the necessary genes in the genome and to identify promising forms. Some of the common strawberry's diseases, causing significant damage to strawberry plantations, are anthracnose (Colletotrichum acutatum Simmonds) and red stele root rot (Phytophthora fragariae var. fragariae Hickman). Dominant Rca2 gene is involved in monogenic resistance to C. acutatum pathogenicity group 2. Rpf1, Rpf2, Rpf3 genes are determined in monogenic resistance to red stele root rot. The purpose of this study was molecular genetic testing genotypes of genus Fragaria L. to identify carriers of Rca2 allele anthracnose resistance and Rpf1 allele red stele root rot resistance. The objects of study were the wild species of the genus Fragaria L. and strawberry varieties (Fragaria x ananassa Duch.) of different ecological and geographic origin. To assess allelic state Rca2 anthracnose resistance gene the dominant SCAR marker STS-Rca2_240 was used, was linked to the resistance gene Rca2 with a genetic distance of 2.8 cM. Rpf1 gene red stele root rot resistance was identified with the dominant SCAR marker R1A, was linked to the resistance gene Rpf1 with a genetic distance of 3.0 cM. The resistant allele of the marker STS-Rca2_240 was identified in the Laetitia variety (Rca2Rca2 or Rca2rca2 genotype), which allows us to recommend it as a promising source in breeding for anthracnose resistance. The other studied forms have homozygous recessive state of the marker STS-Rca2_240 (putative genotype rca2rca2). The resistant allele of the marker SCAR-R1A in the varieties and wild species of strawberry under study is absent, which presumably indicates their homozygous recessive genotype of Rpf1 gene (rpf1rpf1).

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The authors declare that there are no conflicts of interest present.