vavilovВавиловский журнал генетики и селекцииVavilov Journal of Genetics and Breeding2500-3259Institute of Cytology and Genetics of Siberian Branch of the RAS10.18699/VJ19.461vavilov-1868Research ArticleГЕНЕТИКА ЖИВОТНЫХANIMAL GENETICSВлияние мутации lethal yellow (AY) и изменений фотопериода на поведение мышиEffect of lethal yellow (AY) mutation and photoperiod alterations on mouse behaviorБаженоваЕ. Ю.BazhenovaE. Y.

Новосибирск

Novosibirsk

ФурсенкоД. В.FursenkoD. V.

Новосибирск

Novosibirsk

ХоцкинН. В.KhotskinN. V.

Новосибирск

Novosibirsk

СорокинИ. Е.SorokinI. E.

Новосибирск

Novosibirsk

КуликовА. В.KulikovA. V.

Новосибирск

Novosibirsk

v_kulikov@bionet.nsc.ruФедеральный исследовательский центр Институт цитологии и генетики Сибирского отделения Российской академии наукРоссияInstitute of Cytology and Genetics, SB RASRussian Federation2019250220192315561Copyright © Баженова Е.Ю., Фурсенко Д.В., Хоцкин Н.В., Сорокин И.Е., Куликов А.В., 20192019Баженова Е.Ю., Фурсенко Д.В., Хоцкин Н.В., Сорокин И.Е., Куликов А.В.Bazhenova E.Y., Fursenko D.V., Khotskin N.V., Sorokin I.E., Kulikov A.V.This work is licensed under a Creative Commons Attribution 4.0 License.https://vavilov.elpub.ru/jour/article/view/1868

Снижение естественного освещения в осеннее/зимнее время вызывает депрессивно-подобные сезонные аффективные расстройства у предрасположенных индивидов. Ожирение является другим фактором риска депрессии. Мутация lethal yellow (AY) вызывает эктопическую экспрессию белка агути в мозге. Мыши, гетерозиготные по мутации AY (AY/a), страдают ожирением по сравнению с их однопометниками дикого типа (a/a). Основная цель работы – исследование влияния мутации AY, фотопериода и взаимодействия между этими факторами на суточную динамику активности, потребление пищи, локомоторную и исследовательскую активность, тревожность и депрессивно-подобное поведение в условиях умеренного стресса. Самцы AY/a и a/a возрастом 6 недель были разделены на четыре группы и содержались 28 дней при длинном (14 ч день:10 ч ночь) и коротком (4 ч день:20 ч ночь) фотопериоде. Затем поведение этих мышей последовательно исследовали в домашней клетке, тестах «открытое поле», «приподнятый крестообразный лабиринт» и «принудительное плавание». Мы не обнаружили влияния мутации AY на общую активность, потребление пищи и воды в домашней клетке; двигательную активность, исследовательское поведение в тесте «открытое поле»; тревожность в тестах «открытое поле» и «приподнятый крестообразный лабиринт». В то же время мутация AY усиливает депрессивно-подобное поведение в тесте «принудительное плавание» (F1.28 = 20.03, p = 0.00012). Короткий день снижал ночную активность мышей в домашней клетке, как и локомоторную (F1.28 = 16.33, p = 0.0004) и исследовательскую (F1.28 = 16.24, p < 0.0004) активность в тесте «открытое поле». Более того, короткий день снижал время в центре в тесте «открытое поле» (F1.28 = 6.57, p = 0.016) и в открытых рукавах в тесте «приподнятый крестообразный лабиринт» (F1.28 = 12.08, p = 0.0017), а также увеличивал время неподвижности в тесте «принудительное плавание» (F1.28 = 9.95, p = 0.0038). При этом не выявлено влияния взаимодействия генотип × фотопериод на выраженность этих признаков. Следовательно, мутация AY и короткий фотопериод усиливают депрессивно-подобное поведение в тесте «принудительное плавание» посредством различных механизмов.

Decrease in natural illumination in fall/winter months causes depressive-like seasonal affective disorders in vulnerable individuals. Obesity is another risk factor of depression. The lethal yellow (AY) mutation causes ectopic expression of agouti protein in the brain. Mice heterozygous for AY mutation (AY/a) are obese compared to their wild-type littermates (a/a). The main aims of the study were to investigate the effects of AY mutation, photoperiod and the interaction between these factors on daily activity dynamics, feeding, locomotor and exploratory activities, anxiety-related and depressive-like behaviors in mild stress condition. Six weeks old mouse males of AY/a and a/a lines were divided into four groups eight animals each and exposed to long- (14 h light and 10 h darkness) or short- (4 h light and 20 h darkness) day conditions for 28 days. Then the behavior of these mice was successively investigated in the home cage, open field, elevated plus-maze and forced swim tests. We did not observed any effect of AY mutation on the general activity, water and food consumption in the home cage; locomotion and exploration in the open field test; anxiety-related behavior in the open field and elevated plus-maze tests. At the same time, AY mutation increased depressive-like immobility time in the forced swim test (F1.28 = 20.03, p = 0.00012). Shortday conditions decreased nocturnal activity in the home cage, as well as locomotion (F1.28 = 16.33, p = 0.0004) and exploration (F1.28 = 16.24, p < 0.0004) in the open field test. Moreover, short-day exposition decreased time spent in the center of the open field (F1.28 = 6.57, p = 0.016) and in the open arms of the elevated plus-maze (F1.28 = 12.08, p = 0.0017) tests and increased immobility time in the forced swim test (F1.28 = 9.95, p = 0.0038). However, no effect of the interaction between AY mutation and photoperiod on immobility time in the forced swim test was observed. Therefore, short-day photoperiod and AY mutation increased depressive-like behavior in the forced swim test by means of different mechanisms.

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The authors declare that there are no conflicts of interest present.