vavilovВавиловский журнал генетики и селекцииVavilov Journal of Genetics and Breeding2500-3259Institute of Cytology and Genetics of Siberian Branch of the RASvavilov-187Research ArticleСтатьиArticlesКОМПЬЮТЕРНЫЙ АНАЛИЗ ДАННЫХ ЭКСПРЕССИИ ГЕНОВ В КЛЕТКАХ МОЗГА, ПОЛУЧЕННЫХ С ПОМОЩЬЮ МИКРОЧИПОВ И ВЫСОКОПРОИЗВОДИТЕЛЬНОГО СЕКВЕНИРОВАНИЯCOMPUTER ANALYSIS OF DATA ON GENE EXPRESSION IN BRAIN CELLS OBTAINED BY MICROARRAY TESTS AND HIGH-THROUGHPUT SEQUENCINGМедведеваИ. В.MedvedevaI. V.

 

orlov@bionet.nsc.ruВишневскийО. В.VishnevskyO. V.orlov@bionet.nsc.ruСафроноваН. С.SafronovaN. S.orlov@bionet.nsc.ruКожевниковаО. С.KozhevnikovaO. S.orlov@bionet.nsc.ruГенаевМ. А.GenaevM. A.orlov@bionet.nsc.ruАфонниковД. А.KochetovA. V.orlov@bionet.nsc.ruКочетовА. В.AfonnikovD. A.orlov@bionet.nsc.ruОрловЮ. Л.OrlovY. L.orlov@bionet.nsc.ruФедеральное государственное бюджетное научное учреждение «Федеральный исследовательский центр Институт цитологии и генетики Сибирского отделения Российской академии наук», Новосибирск, РоссияРоссияInstitute of Cytology and Genetics SB RAS, Novosibirsk, RussiaRussian Federation201310012015174/1629638Copyright © Медведева И.В., Вишневский О.В., Сафронова Н.С., Кожевникова О.С., Генаев М.А., Афонников Д.А., Кочетов А.В., Орлов Ю.Л., 20152015Медведева И.В., Вишневский О.В., Сафронова Н.С., Кожевникова О.С., Генаев М.А., Афонников Д.А., Кочетов А.В., Орлов Ю.Л.Medvedeva I.V., Vishnevsky O.V., Safronova N.S., Kozhevnikova O.S., Genaev M.A., Kochetov A.V., Afonnikov D.A., Orlov Y.L.This work is licensed under a Creative Commons Attribution 4.0 License.https://vavilov.elpub.ru/jour/article/view/187

В последние годы происходит стремительное расширение фронта нейробиологических исследований, сопровождающееся бурным ростом объема экспериментальных данных по структуре, функции и эволюции нервной системы на различных уровнях ее иерархической организации. Использование технологий высокопроизводительного секвенирования и микрочипов позволяет проводить сравнительный статистический анализ экспрессии тысяч генов одновременно, учитывая при этом пространственное расположение клеток в структурах мозга. Дан краткий обзор основных подходов анализа экспрессии генов в клетках мозга. Проанализированы особенности структуры генов, имеющих дифференциальную экспрессию в клетках мозга. Оценивалось число экзонов, альтернативных транскриптов и его соотношение с уровнем экспрессии. Показано статистическое различие числа альтернативных транскриптов для генов, активных в структурах головного мозга и других органов. Найдены гены, экспрессия которых повышена в структурах мозга и связана с нейродегенеративными заболеваниями.

The scope of neurobiological studies has been greatly expanded in the last years. It is accompanied by accumulation of a huge body of experimental data on the structure, function and evolution of the nervous system at different hierarchical levels of its organization. High-throughput sequencing technologies and microarray tests permit the expression of thousands of genes to be analyzed with regard to cell location in the brain. Methods of gene expression analysis are briefly reviewed in the context of brain research. We have analyzed specific features of genes differentially expressed in brain cells. Some genes overexpressed in brain tissues are associated with neurological diseases. The numbers of exons and active transcripts in genes differentially expressed in different organs are considered. Statistically significant difference in such parameters is shown for genes intensely expressed in the brain and other organs. Examples of such differentially expressed genes associated with neurological deceases are presented.

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The authors declare that there are no conflicts of interest present.