<?xml version="1.0" encoding="UTF-8"?>
<!DOCTYPE article PUBLIC "-//NLM//DTD JATS (Z39.96) Journal Publishing DTD v1.3 20210610//EN" "JATS-journalpublishing1-3.dtd">
<article article-type="research-article" dtd-version="1.3" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:lang="ru"><front><journal-meta><journal-id journal-id-type="publisher-id">vavilov</journal-id><journal-title-group><journal-title xml:lang="ru">Вавиловский журнал генетики и селекции</journal-title><trans-title-group xml:lang="en"><trans-title>Vavilov Journal of Genetics and Breeding</trans-title></trans-title-group></journal-title-group><issn pub-type="epub">2500-3259</issn><publisher><publisher-name>Institute of Cytology and Genetics of Siberian Branch of the RAS</publisher-name></publisher></journal-meta><article-meta><article-id custom-type="elpub" pub-id-type="custom">vavilov-192</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="ru"><subject>Статьи</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="en"><subject>Articles</subject></subj-group></article-categories><title-group><article-title>СОВРЕМЕННОЕ СОСТОЯНИЕ ИССЛЕДОВАНИЙ В ОБЛАСТИ ГЕНЕТИЧЕСКОЙ И МЕТАБОЛИЧЕСКОЙ ИНЖЕНЕРИИ БАКТЕРИЙ РОДА Geobacillus, НАПРАВЛЕННЫХ НА ПОЛУЧЕНИЕ ЭТАНОЛА И ОРГАНИЧЕСКИХ КИСЛОТ</article-title><trans-title-group xml:lang="en"><trans-title>THE CURRENT STATE OF GENETIC AND METABOLIC ENGINEERING OF Geobacillus BACTERIA AIMED AT THE PRODUCTION OF ETHANOL AND ORGANIC ACIDS</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Розанов</surname><given-names>А. С.</given-names></name><name name-style="western" xml:lang="en"><surname>Rozanov</surname><given-names>A. S.</given-names></name></name-alternatives><email xlink:type="simple">miren@bionet.nsc.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Мещерякова</surname><given-names>И. А.</given-names></name><name name-style="western" xml:lang="en"><surname>Meshcheryakova</surname><given-names>I. A.</given-names></name></name-alternatives><email xlink:type="simple">miren@bionet.nsc.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Шеховцов</surname><given-names>С. В.</given-names></name><name name-style="western" xml:lang="en"><surname>Shekhovtsov</surname><given-names>S. V.</given-names></name></name-alternatives><email xlink:type="simple">miren@bionet.nsc.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Пельтек</surname><given-names>С. Е.</given-names></name><name name-style="western" xml:lang="en"><surname>Peltek</surname><given-names>S. E.</given-names></name></name-alternatives><email xlink:type="simple">miren@bionet.nsc.ru</email><xref ref-type="aff" rid="aff-1"/></contrib></contrib-group><aff-alternatives id="aff-1"><aff xml:lang="ru">Федеральное государственное бюджетное научное учреждение «Федеральный исследовательский центр Институт цитологии и генетики Сибирского отделения Российской академии наук», Новосибирск, Россия<country>Россия</country></aff><aff xml:lang="en">Institute of Cytology and Genetics SB RAS, Novosibirsk, Russia<country>Russian Federation</country></aff></aff-alternatives><pub-date pub-type="collection"><year>2013</year></pub-date><pub-date pub-type="epub"><day>10</day><month>01</month><year>2015</year></pub-date><volume>17</volume><issue>4/1</issue><fpage>675</fpage><lpage>685</lpage><permissions><copyright-statement>Copyright &amp;#x00A9; Розанов А.С., Мещерякова И.А., Шеховцов С.В., Пельтек С.Е., 2015</copyright-statement><copyright-year>2015</copyright-year><copyright-holder xml:lang="ru">Розанов А.С., Мещерякова И.А., Шеховцов С.В., Пельтек С.Е.</copyright-holder><copyright-holder xml:lang="en">Rozanov A.S., Meshcheryakova I.A., Shekhovtsov S.V., Peltek S.E.</copyright-holder><license license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>This work is licensed under a Creative Commons Attribution 4.0 License.</license-p></license></permissions><self-uri xlink:href="https://vavilov.elpub.ru/jour/article/view/192">https://vavilov.elpub.ru/jour/article/view/192</self-uri><abstract><p>Термофильные бактерии находят все более широкое применение в биотехнологии. Одними из наиболее перспективных термофилов являются представители рода Geobacillus. В статье рассмотрены известные на данный момент методики генетической и метаболической инженерии этих микроорганизмов, а также примеры их использования в различных отраслях биотехнологии.</p></abstract><trans-abstract xml:lang="en"><p>Thermophilic bacteria are extensively used in biotechnology. Species of the genus Geobacillus rank among the most promising ones. Current methods of the genetic and metabolic engineering of these microorganisms are considered. Examples of their use in various branches of biotechnology are presented.</p></trans-abstract><kwd-group xml:lang="ru"><kwd>Geobacillus</kwd><kwd>термофилы</kwd><kwd>биотехнология</kwd><kwd>генетическая инженерия</kwd></kwd-group><kwd-group xml:lang="en"><kwd>Geobacillus</kwd><kwd>thermophiles</kwd><kwd>biotechnology</kwd><kwd>genetic engineering</kwd></kwd-group><funding-group xml:lang="ru"><funding-statement>грант № 14.512.11.0057 Министерства образования и науки Российской Федерации</funding-statement></funding-group></article-meta></front><back><ref-list><title>References</title><ref id="cit1"><label>1</label><citation-alternatives><mixed-citation xml:lang="ru">Abdel-Fattah Y.R., Gaballa A.A. Identification and overexpression of a thermostable lipase from Geobacillus thermoleovorans Toshki in Escherichia coli // Microbiol. Res. 2008. V. 163. P. 13–20.</mixed-citation><mixed-citation xml:lang="en">Abdel-Fattah Y.R., Gaballa A.A. Identification and overexpression of a thermostable lipase from Geobacillus thermoleovorans Toshki in Escherichia coli // Microbiol. Res. 2008. V. 163. P. 13–20.</mixed-citation></citation-alternatives></ref><ref id="cit2"><label>2</label><citation-alternatives><mixed-citation xml:lang="ru">Afzal M., Oommen S., Al-Awadi S. Transformation of chenodeoxycholic acid by thermophilic Geobacillus stearothermophilus // Biotechnol. Appl. Biochem. 2011. V. 58. P. 250–255.</mixed-citation><mixed-citation xml:lang="en">Afzal M., Oommen S., Al-Awadi S. Transformation of chenodeoxycholic acid by thermophilic Geobacillus stearothermophilus // Biotechnol. Appl. Biochem. 2011. V. 58. P. 250–255.</mixed-citation></citation-alternatives></ref><ref id="cit3"><label>3</label><citation-alternatives><mixed-citation xml:lang="ru">Alterthum F., Ingram L.O. Efficient ethanol production from glucose, lactose, and xylose by recombinant Escherichia coli // Appl. Envir. Microbiol. 1989. V. 55. P. 1943–1948.</mixed-citation><mixed-citation xml:lang="en">Alterthum F., Ingram L.O. Efficient ethanol production from glucose, lactose, and xylose by recombinant Escherichia coli // Appl. Envir. Microbiol. 1989. V. 55. P. 1943–1948.</mixed-citation></citation-alternatives></ref><ref id="cit4"><label>4</label><citation-alternatives><mixed-citation xml:lang="ru">Ash C., Farrow J.A.E., Wallbanks S., Collins M.D. Phylogenetic heterogeneity of the genus Bacillus revealed by comparative analysis of small-subunit-ribosomal RNA sequences // Lett. Appl. Microbiol. 1991. V. 13. P. 202–206.</mixed-citation><mixed-citation xml:lang="en">Ash C., Farrow J.A.E., Wallbanks S., Collins M.D. Phylogenetic heterogeneity of the genus Bacillus revealed by comparative analysis of small-subunit-ribosomal RNA sequences // Lett. Appl. Microbiol. 1991. V. 13. P. 202–206.</mixed-citation></citation-alternatives></ref><ref id="cit5"><label>5</label><citation-alternatives><mixed-citation xml:lang="ru">Assareh R., Shahbani Zahiri H., Akbari Noghabi K. et al. Characterization of the newly isolated Geobacillus sp. T1, the efficient cellulase-producer on untreated barley and wheat straws // Biores. Technol. 2012. V. 120. P. 99–105.</mixed-citation><mixed-citation xml:lang="en">Assareh R., Shahbani Zahiri H., Akbari Noghabi K. et al. Characterization of the newly isolated Geobacillus sp. T1, the efficient cellulase-producer on untreated barley and wheat straws // Biores. Technol. 2012. V. 120. P. 99–105.</mixed-citation></citation-alternatives></ref><ref id="cit6"><label>6</label><citation-alternatives><mixed-citation xml:lang="ru">Balan A., Ibrahim D., Abdul Rahim R., Ahmad Rashid F.A. Purification and characterization of a thermostable lipase from Geobacillus thermodenitrificans IBRL-nra // Enzyme Res. 2012. V. 2012. P. 987523.</mixed-citation><mixed-citation xml:lang="en">Balan A., Ibrahim D., Abdul Rahim R., Ahmad Rashid F.A. Purification and characterization of a thermostable lipase from Geobacillus thermodenitrificans IBRL-nra // Enzyme Res. 2012. V. 2012. P. 987523.</mixed-citation></citation-alternatives></ref><ref id="cit7"><label>7</label><citation-alternatives><mixed-citation xml:lang="ru">Banat I.M., Marchant R., Rahman T.J. Geobacillus debilis sp. nov., a novel obligately thermophilic bacterium isolated from a cool soil environment, and reassignment of Bacillus pallidus to Geobacillus pallidus comb. nov. // Int. J. Syst. Evol. Microbiol. 2004. V. 54. P. 2197–2201.</mixed-citation><mixed-citation xml:lang="en">Banat I.M., Marchant R., Rahman T.J. Geobacillus debilis sp. nov., a novel obligately thermophilic bacterium isolated from a cool soil environment, and reassignment of Bacillus pallidus to Geobacillus pallidus comb. nov. // Int. J. Syst. Evol. Microbiol. 2004. V. 54. P. 2197–2201.</mixed-citation></citation-alternatives></ref><ref id="cit8"><label>8</label><citation-alternatives><mixed-citation xml:lang="ru">Bartosiak-Jentys J., Eley K., Leak D.J. Application of pheB as a reporter gene for Geobacillus sрp., enabling qualitative colony screening and quantitative analysis of promoter strength // Appl. Envir. Microbiol. 2012. V. 78. P. 5945–5947.</mixed-citation><mixed-citation xml:lang="en">Bartosiak-Jentys J., Eley K., Leak D.J. Application of pheB as a reporter gene for Geobacillus sрp., enabling qualitative colony screening and quantitative analysis of promoter strength // Appl. Envir. Microbiol. 2012. V. 78. P. 5945–5947.</mixed-citation></citation-alternatives></ref><ref id="cit9"><label>9</label><citation-alternatives><mixed-citation xml:lang="ru">Bartosiak-Jentys J., Hussein A.H., Lewis C.J., Leak D.J. Modular system for assessment of glycosyl hydrolase secretion in Geobacillus thermoglucosidasius // Microbiology. 2013. V. 159. P. 1267–1275.</mixed-citation><mixed-citation xml:lang="en">Bartosiak-Jentys J., Hussein A.H., Lewis C.J., Leak D.J. Modular system for assessment of glycosyl hydrolase secretion in Geobacillus thermoglucosidasius // Microbiology. 2013. V. 159. P. 1267–1275.</mixed-citation></citation-alternatives></ref><ref id="cit10"><label>10</label><citation-alternatives><mixed-citation xml:lang="ru">Ben-David A., Bravman T., Balazs Y.S. et al. Glycosynthase activity of Geobacillus stearothermophilus GH52 betaxylosidase: efficient synthesis of xylooligosaccharides from alpha-D-xylopyranosyl fluoride through a conjugated reaction // Eur. J. Chem. Biol. 2007. V. 8. P. 2145–2151.</mixed-citation><mixed-citation xml:lang="en">Ben-David A., Bravman T., Balazs Y.S. et al. Glycosynthase activity of Geobacillus stearothermophilus GH52 betaxylosidase: efficient synthesis of xylooligosaccharides from alpha-D-xylopyranosyl fluoride through a conjugated reaction // Eur. J. Chem. Biol. 2007. V. 8. P. 2145–2151.</mixed-citation></citation-alternatives></ref><ref id="cit11"><label>11</label><citation-alternatives><mixed-citation xml:lang="ru">Canakci S., Inan K., Kacagan M., Belduz A.O. Evaluation of arabinofuranosidase and xylanase activities of Geobacillus sрp. isolated from some hot springs in Turkey // J. Microbiol. Biotechnol. 2007. V. 17. P. 1262–1270.</mixed-citation><mixed-citation xml:lang="en">Canakci S., Inan K., Kacagan M., Belduz A.O. Evaluation of arabinofuranosidase and xylanase activities of Geobacillus sрp. isolated from some hot springs in Turkey // J. Microbiol. Biotechnol. 2007. V. 17. P. 1262–1270.</mixed-citation></citation-alternatives></ref><ref id="cit12"><label>12</label><citation-alternatives><mixed-citation xml:lang="ru">Canakci S., Cevher Z., Inan K. et al. Cloning, purification and characterization of an alkali-stable endoxylanase from thermophilic Geobacillus sp. 71 // World J. Microbiol. Biotechnol. 2012. V. 28. P. 1981–1988.</mixed-citation><mixed-citation xml:lang="en">Canakci S., Cevher Z., Inan K. et al. Cloning, purification and characterization of an alkali-stable endoxylanase from thermophilic Geobacillus sp. 71 // World J. Microbiol. Biotechnol. 2012. V. 28. P. 1981–1988.</mixed-citation></citation-alternatives></ref><ref id="cit13"><label>13</label><citation-alternatives><mixed-citation xml:lang="ru">Chang S., Cohen S.N. High frequency transformation of Bacillus subtilis protoplasts by plasmid DNA // Mol. Gen. Genet. 1979. V. 168. P. 111–115.</mixed-citation><mixed-citation xml:lang="en">Chang S., Cohen S.N. High frequency transformation of Bacillus subtilis protoplasts by plasmid DNA // Mol. Gen. Genet. 1979. V. 168. P. 111–115.</mixed-citation></citation-alternatives></ref><ref id="cit14"><label>14</label><citation-alternatives><mixed-citation xml:lang="ru">Cheong K.W., Leow T.C., Rahman R.N.Z.R.A. et al. Reductive alkylation causes the formation of a molten globule-like intermediate structure in Geobacillus zalihae strain T1 thermostable lipase // Appl. Biochem. Biotechnol. 2011. V. 164. P. 362–375.</mixed-citation><mixed-citation xml:lang="en">Cheong K.W., Leow T.C., Rahman R.N.Z.R.A. et al. Reductive alkylation causes the formation of a molten globule-like intermediate structure in Geobacillus zalihae strain T1 thermostable lipase // Appl. Biochem. Biotechnol. 2011. V. 164. P. 362–375.</mixed-citation></citation-alternatives></ref><ref id="cit15"><label>15</label><citation-alternatives><mixed-citation xml:lang="ru">Coorevits A., Logan N.A., Dinsdale A.E. et al. Bacillus thermolactis sp. nov., isolated from dairy farms, and emended description of Bacillus thermoamylovorans // Int. J. Syst. Evol. Microbiol. 2011. V. 61. P. 1954–1961.</mixed-citation><mixed-citation xml:lang="en">Coorevits A., Logan N.A., Dinsdale A.E. et al. Bacillus thermolactis sp. nov., isolated from dairy farms, and emended description of Bacillus thermoamylovorans // Int. J. Syst. Evol. Microbiol. 2011. V. 61. P. 1954–1961.</mixed-citation></citation-alternatives></ref><ref id="cit16"><label>16</label><citation-alternatives><mixed-citation xml:lang="ru">Cripps R.E., Eley K., Leak D.J. et al. Metabolic engineering of Geobacillus thermoglucosidasius for high yield ethanol production // Metab. Eng. 2009. V. 11. P. 398–408.</mixed-citation><mixed-citation xml:lang="en">Cripps R.E., Eley K., Leak D.J. et al. Metabolic engineering of Geobacillus thermoglucosidasius for high yield ethanol production // Metab. Eng. 2009. V. 11. P. 398–408.</mixed-citation></citation-alternatives></ref><ref id="cit17"><label>17</label><citation-alternatives><mixed-citation xml:lang="ru">De Benedetti E.C., Rivero C.W., Britos C.N. et al. Biotransformation of 2,6-diaminopurine nucleosides by immobilized Geobacillus stearothermophilus // Biotechnol. Progr. 2012. V. 28. P. 1251–1256.</mixed-citation><mixed-citation xml:lang="en">De Benedetti E.C., Rivero C.W., Britos C.N. et al. Biotransformation of 2,6-diaminopurine nucleosides by immobilized Geobacillus stearothermophilus // Biotechnol. Progr. 2012. V. 28. P. 1251–1256.</mixed-citation></citation-alternatives></ref><ref id="cit18"><label>18</label><citation-alternatives><mixed-citation xml:lang="ru">De Rossi E., Brigidi P., Rossi M. et al. Characterization of grampositive broad host-range plasmids carrying a thermophilic replicon // Res. Microbiol. 1991. V. 142. P. 389–396.</mixed-citation><mixed-citation xml:lang="en">De Rossi E., Brigidi P., Rossi M. et al. Characterization of grampositive broad host-range plasmids carrying a thermophilic replicon // Res. Microbiol. 1991. V. 142. P. 389–396.</mixed-citation></citation-alternatives></ref><ref id="cit19"><label>19</label><citation-alternatives><mixed-citation xml:lang="ru">Dinsdale A.E., Halket G., Coorevits A. et al. Emended descriptions of Geobacillus thermoleovorans and Geobacillus thermocatenulatus // Int. J. Syst. Evol. Microbiol. 2011. V. 61. P. 1802–1810.</mixed-citation><mixed-citation xml:lang="en">Dinsdale A.E., Halket G., Coorevits A. et al. Emended descriptions of Geobacillus thermoleovorans and Geobacillus thermocatenulatus // Int. J. Syst. Evol. Microbiol. 2011. V. 61. P. 1802–1810.</mixed-citation></citation-alternatives></ref><ref id="cit20"><label>20</label><citation-alternatives><mixed-citation xml:lang="ru">Donk P.J. A highly resistant thermophilic organism // J. Bacteriol. 1920. V. 5. P. 373–374.</mixed-citation><mixed-citation xml:lang="en">Donk P.J. A highly resistant thermophilic organism // J. Bacteriol. 1920. V. 5. P. 373–374.</mixed-citation></citation-alternatives></ref><ref id="cit21"><label>21</label><citation-alternatives><mixed-citation xml:lang="ru">Ebrahimpour A., Rahman R.N., Basri M., Salleh A.B. High level expression and characterization of a novel thermostable, organic solvent tolerant, 1,3-regioselective lipase from Geobacillus sp. strain ARM // Biores. Technol. 2011. V. 102. P. 6972–6981.</mixed-citation><mixed-citation xml:lang="en">Ebrahimpour A., Rahman R.N., Basri M., Salleh A.B. High level expression and characterization of a novel thermostable, organic solvent tolerant, 1,3-regioselective lipase from Geobacillus sp. strain ARM // Biores. Technol. 2011. V. 102. P. 6972–6981.</mixed-citation></citation-alternatives></ref><ref id="cit22"><label>22</label><citation-alternatives><mixed-citation xml:lang="ru">Fong J.C.N., Svenson C.J., Nakasugi K. et al. Isolation and characterization of two novel ethanol-tolerant facultative-anaerobic thermophilic bacteria strains from waste compost // Extremophiles. 2006. V. 10. P. 363–372.</mixed-citation><mixed-citation xml:lang="en">Fong J.C.N., Svenson C.J., Nakasugi K. et al. Isolation and characterization of two novel ethanol-tolerant facultative-anaerobic thermophilic bacteria strains from waste compost // Extremophiles. 2006. V. 10. P. 363–372.</mixed-citation></citation-alternatives></ref><ref id="cit23"><label>23</label><citation-alternatives><mixed-citation xml:lang="ru">Fortina M.G., Mora D., Schumann P. et al. Reclassification of Saccharococcus caldoxylosilyticus as Geobacillus caldoxylosilyticus (Ahmad et al. 2000) comb. nov. // Int. J. Syst. Evol. Microbiol. 2001. V. 51. P. 2063–2071.</mixed-citation><mixed-citation xml:lang="en">Fortina M.G., Mora D., Schumann P. et al. Reclassification of Saccharococcus caldoxylosilyticus as Geobacillus caldoxylosilyticus (Ahmad et al. 2000) comb. nov. // Int. J. Syst. Evol. Microbiol. 2001. V. 51. P. 2063–2071.</mixed-citation></citation-alternatives></ref><ref id="cit24"><label>24</label><citation-alternatives><mixed-citation xml:lang="ru">Gerasimova J., Kuisiene N. Characterization of the novel xylanase from the thermophilic Geobacillus thermodenitrificans JK1 // Mikrobiologiia. 2012. V. 81. P. 457–463.</mixed-citation><mixed-citation xml:lang="en">Gerasimova J., Kuisiene N. Characterization of the novel xylanase from the thermophilic Geobacillus thermodenitrificans JK1 // Mikrobiologiia. 2012. V. 81. P. 457–463.</mixed-citation></citation-alternatives></ref><ref id="cit25"><label>25</label><citation-alternatives><mixed-citation xml:lang="ru">Goh K.M., Kahar U.M., Chai Y.Y. et al. Recent discoveries and applications of Anoxybacillus // Appl. Microbiol. Biotechnol. 2013. V. 97. P. 1475–1488.</mixed-citation><mixed-citation xml:lang="en">Goh K.M., Kahar U.M., Chai Y.Y. et al. Recent discoveries and applications of Anoxybacillus // Appl. Microbiol. Biotechnol. 2013. V. 97. P. 1475–1488.</mixed-citation></citation-alternatives></ref><ref id="cit26"><label>26</label><citation-alternatives><mixed-citation xml:lang="ru">Gordon R.E., Smith N.R. Aerobic sporeforming bacteria capable of growth at high temperatures // J. Bacteriol. 1949. V. 58. P. 327–341.</mixed-citation><mixed-citation xml:lang="en">Gordon R.E., Smith N.R. Aerobic sporeforming bacteria capable of growth at high temperatures // J. Bacteriol. 1949. V. 58. P. 327–341.</mixed-citation></citation-alternatives></ref><ref id="cit27"><label>27</label><citation-alternatives><mixed-citation xml:lang="ru">Hartley B.S., Shama G. Novel ethanol fermentations from sugar cane and straw // Phil. Trans. Royal Soc. A. 1987. V. 321. P. 555–568.</mixed-citation><mixed-citation xml:lang="en">Hartley B.S., Shama G. Novel ethanol fermentations from sugar cane and straw // Phil. Trans. Royal Soc. A. 1987. V. 321. P. 555–568.</mixed-citation></citation-alternatives></ref><ref id="cit28"><label>28</label><citation-alternatives><mixed-citation xml:lang="ru">Imanaka T., Fujii M., Aramori I., Aiba S. Transformation of Bacillus stearothermophilus with plasmid DNA and characterization of shuttle vector plasmids between Bacillus stearothermophilus and Bacillus subtilis // J. Bacteriol. 1982. V. 149. P. 824–830.</mixed-citation><mixed-citation xml:lang="en">Imanaka T., Fujii M., Aramori I., Aiba S. Transformation of Bacillus stearothermophilus with plasmid DNA and characterization of shuttle vector plasmids between Bacillus stearothermophilus and Bacillus subtilis // J. Bacteriol. 1982. V. 149. P. 824–830.</mixed-citation></citation-alternatives></ref><ref id="cit29"><label>29</label><citation-alternatives><mixed-citation xml:lang="ru">Ingram L.O., Conway T., Clark D.P. et al. Genetic engineering of ethanol production in Escherichia coli // Appl. Envir. Microbiol. 1987. V. 53. P. 2420–2425.</mixed-citation><mixed-citation xml:lang="en">Ingram L.O., Conway T., Clark D.P. et al. Genetic engineering of ethanol production in Escherichia coli // Appl. Envir. Microbiol. 1987. V. 53. P. 2420–2425.</mixed-citation></citation-alternatives></ref><ref id="cit30"><label>30</label><citation-alternatives><mixed-citation xml:lang="ru">Kuisiene N., Raugalas J., Chitavichius D. Geobacillus lituanicus sp. nov. // Int. J. Syst. Evol. Microbiol. 2004. V. 54. P. 1991–1995.</mixed-citation><mixed-citation xml:lang="en">Kuisiene N., Raugalas J., Chitavichius D. Geobacillus lituanicus sp. nov. // Int. J. Syst. Evol. Microbiol. 2004. V. 54. P. 1991–1995.</mixed-citation></citation-alternatives></ref><ref id="cit31"><label>31</label><citation-alternatives><mixed-citation xml:lang="ru">Liao H., McKenzie T., Hageman R. Isolation of a thermostable enzyme variant by cloning and selection in a thermophile // Proc. Natl Acad. Sci. USA. 1986. V. 83. P. 576–580.</mixed-citation><mixed-citation xml:lang="en">Liao H., McKenzie T., Hageman R. Isolation of a thermostable enzyme variant by cloning and selection in a thermophile // Proc. Natl Acad. Sci. USA. 1986. V. 83. P. 576–580.</mixed-citation></citation-alternatives></ref><ref id="cit32"><label>32</label><citation-alternatives><mixed-citation xml:lang="ru">Liu B., Zhang N., Zhao C. et al. Characterization of a recombinant thermostable xylanase from hot spring thermophilic Geobacillus sp. TC-W7 // J. Microbiol. Biotechnol. 2012. V. 22. P. 1388–1394.</mixed-citation><mixed-citation xml:lang="en">Liu B., Zhang N., Zhao C. et al. Characterization of a recombinant thermostable xylanase from hot spring thermophilic Geobacillus sp. TC-W7 // J. Microbiol. Biotechnol. 2012. V. 22. P. 1388–1394.</mixed-citation></citation-alternatives></ref><ref id="cit33"><label>33</label><citation-alternatives><mixed-citation xml:lang="ru">Miñana-Galbis D., Pinzón D.L., Lorén J.G. et al. Reclassification of Geobacillus pallidus (Scholz et al., 1988) Banat et al. 2004 as Aeribacillus pallidus gen. nov., comb. nov. // Int. J. Syst. Evol. Microbiol. 2010. V. 60. P. 1600–1604.</mixed-citation><mixed-citation xml:lang="en">Miñana-Galbis D., Pinzón D.L., Lorén J.G. et al. Reclassification of Geobacillus pallidus (Scholz et al., 1988) Banat et al. 2004 as Aeribacillus pallidus gen. nov., comb. nov. // Int. J. Syst. Evol. Microbiol. 2010. V. 60. P. 1600–1604.</mixed-citation></citation-alternatives></ref><ref id="cit34"><label>34</label><citation-alternatives><mixed-citation xml:lang="ru">Narumi I., Sawakami K., Nakamoto S. et al. A newly isolated Bacillus stearotheromophilus K1041 and its transformation by electroporation // Biotechnol. Techn. 1992. V. 6. P. 83–86.</mixed-citation><mixed-citation xml:lang="en">Narumi I., Sawakami K., Nakamoto S. et al. A newly isolated Bacillus stearotheromophilus K1041 and its transformation by electroporation // Biotechnol. Techn. 1992. V. 6. P. 83–86.</mixed-citation></citation-alternatives></ref><ref id="cit35"><label>35</label><citation-alternatives><mixed-citation xml:lang="ru">Nazina T.N., Tourova T.P., Poltaraus A.B. et al. Taxonomic study of aerobic thermophilic bacilli: descriptions of Geobacillus subterraneus gen. nov., sp. nov. and Geobacillus uzenensis sp. nov. from petroleum reservoirs and transfer of Bacillus stearothermophilus, Bacillus thermocatenulatus, Bacillus</mixed-citation><mixed-citation xml:lang="en">Nazina T.N., Tourova T.P., Poltaraus A.B. et al. Taxonomic study of aerobic thermophilic bacilli: descriptions of Geobacillus subterraneus gen. nov., sp. nov. and Geobacillus uzenensis sp. nov. from petroleum reservoirs and transfer of Bacillus stearothermophilus, Bacillus thermocatenulatus, Bacillus</mixed-citation></citation-alternatives></ref><ref id="cit36"><label>36</label><citation-alternatives><mixed-citation xml:lang="ru">thermoleovorans, Bacillus kaustophilus, Bacillus thermoglucosidasius and Bacillus thermodenitrificans to Geobacillus as the new combinations G. stearothermophilus, G. thermocatenulatus, G. thermoleovorans, G. kaustophilus, G. thermoglucosidasius and G. thermodenitrificans // Int. J. Syst. Evol. Microbiol. 2001. V. 51. P. 433–446.</mixed-citation><mixed-citation xml:lang="en">thermoleovorans, Bacillus kaustophilus, Bacillus thermoglucosidasius and Bacillus thermodenitrificans to Geobacillus as the new combinations G. stearothermophilus, G. thermocatenulatus, G. thermoleovorans, G. kaustophilus, G. thermoglucosidasius and G. thermodenitrificans // Int. J. Syst. Evol. Microbiol. 2001. V. 51. P. 433–446.</mixed-citation></citation-alternatives></ref><ref id="cit37"><label>37</label><citation-alternatives><mixed-citation xml:lang="ru">Nazina T.N., Lebedeva E.V., Poltaraus A.B. et al. Geobacillus gargensis sp. nov., a novel thermophile from a hot spring, and the reclassification of Bacillus vulcani as Geobacillus vulcani comb. nov. // Int. J. Syst. Evol. Microbiol. 2004. V. 54. P. 2019–2024.</mixed-citation><mixed-citation xml:lang="en">Nazina T.N., Lebedeva E.V., Poltaraus A.B. et al. Geobacillus gargensis sp. nov., a novel thermophile from a hot spring, and the reclassification of Bacillus vulcani as Geobacillus vulcani comb. nov. // Int. J. Syst. Evol. Microbiol. 2004. V. 54. P. 2019–2024.</mixed-citation></citation-alternatives></ref><ref id="cit38"><label>38</label><citation-alternatives><mixed-citation xml:lang="ru">Ng I.-S., Li C.-W., Yeh Y.-F. et al. A novel endo-glucanase from the thermophilic bacterium Geobacillus sp. 70PC53 with high activity and stability over a broad range of temperatures // Extremophiles. 2009. V. 13. P. 425–435.</mixed-citation><mixed-citation xml:lang="en">Ng I.-S., Li C.-W., Yeh Y.-F. et al. A novel endo-glucanase from the thermophilic bacterium Geobacillus sp. 70PC53 with high activity and stability over a broad range of temperatures // Extremophiles. 2009. V. 13. P. 425–435.</mixed-citation></citation-alternatives></ref><ref id="cit39"><label>39</label><citation-alternatives><mixed-citation xml:lang="ru">Payton M.A., Hartley B.S. Mutants of Bacillus stearothermophilus lacking NAD-linked l-lactate dehydrogenase // FEMS Microbiol. Lett. 1985. V. 26. P. 333–336.</mixed-citation><mixed-citation xml:lang="en">Payton M.A., Hartley B.S. Mutants of Bacillus stearothermophilus lacking NAD-linked l-lactate dehydrogenase // FEMS Microbiol. Lett. 1985. V. 26. P. 333–336.</mixed-citation></citation-alternatives></ref><ref id="cit40"><label>40</label><citation-alternatives><mixed-citation xml:lang="ru">Quintana-Castro R., Díaz P., Valerio-Alfaro G. et al. Gene cloning, expression, and characterization of the Geobacillus thermoleovorans CCR11 thermoalkaliphilic lipase // Mol. Biotechnol. 2009. V. 42. P. 75–83.</mixed-citation><mixed-citation xml:lang="en">Quintana-Castro R., Díaz P., Valerio-Alfaro G. et al. Gene cloning, expression, and characterization of the Geobacillus thermoleovorans CCR11 thermoalkaliphilic lipase // Mol. Biotechnol. 2009. V. 42. P. 75–83.</mixed-citation></citation-alternatives></ref><ref id="cit41"><label>41</label><citation-alternatives><mixed-citation xml:lang="ru">Ratnadewi A.A.I., Fanani M., Kurniasih S.D. et al. β-DXylosidase from Geobacillus thermoleovorans IT-08: biochemical characterization and bioinformatics of the enzyme // Appl. Biochem. Biotechnol. 2013. V. 170. No. 8. P. 1950–1964.</mixed-citation><mixed-citation xml:lang="en">Ratnadewi A.A.I., Fanani M., Kurniasih S.D. et al. β-DXylosidase from Geobacillus thermoleovorans IT-08: biochemical characterization and bioinformatics of the enzyme // Appl. Biochem. Biotechnol. 2013. V. 170. No. 8. P. 1950–1964.</mixed-citation></citation-alternatives></ref><ref id="cit42"><label>42</label><citation-alternatives><mixed-citation xml:lang="ru">Riyanti E.I., Rogers P.L. Kinetic evaluation of ethanol-tolerant thermophile Geobacillus thermoglucosidasius M10exg for ethanol production // Indones. J. Agric. Sci. 2009. V. 10. No. 1. P. 34–41.</mixed-citation><mixed-citation xml:lang="en">Riyanti E.I., Rogers P.L. Kinetic evaluation of ethanol-tolerant thermophile Geobacillus thermoglucosidasius M10exg for ethanol production // Indones. J. Agric. Sci. 2009. V. 10. No. 1. P. 34–41.</mixed-citation></citation-alternatives></ref><ref id="cit43"><label>43</label><citation-alternatives><mixed-citation xml:lang="ru">Shallom D., Leon M., Bravman T. et al. Biochemical characterization and identification of the catalytic residues of a family 43 beta-D-xylosidase from Geobacillus stearothermophilus T-6 // Biochemistry. 2005. V. 44. P. 387–397.</mixed-citation><mixed-citation xml:lang="en">Shallom D., Leon M., Bravman T. et al. Biochemical characterization and identification of the catalytic residues of a family 43 beta-D-xylosidase from Geobacillus stearothermophilus T-6 // Biochemistry. 2005. V. 44. P. 387–397.</mixed-citation></citation-alternatives></ref><ref id="cit44"><label>44</label><citation-alternatives><mixed-citation xml:lang="ru">Sung M.H., Kim H., Bae J.W. et al. Geobacillus toebii sp. nov., a novel thermophilic bacterium isolated from hay compost // Int. J. Syst. Evol. Microbiol. 2002. V. 52. P. 2251–2255.</mixed-citation><mixed-citation xml:lang="en">Sung M.H., Kim H., Bae J.W. et al. Geobacillus toebii sp. nov., a novel thermophilic bacterium isolated from hay compost // Int. J. Syst. Evol. Microbiol. 2002. V. 52. P. 2251–2255.</mixed-citation></citation-alternatives></ref><ref id="cit45"><label>45</label><citation-alternatives><mixed-citation xml:lang="ru">Suzuki H., Yoshida K.-I. Genetic transformation of Geobacillus kaustophilus HTA426 by conjugative transfer of host-mimicking plasmids // J. Microbiol. Biotechnol. 2012. V. 22. P. 1279–1287.</mixed-citation><mixed-citation xml:lang="en">Suzuki H., Yoshida K.-I. Genetic transformation of Geobacillus kaustophilus HTA426 by conjugative transfer of host-mimicking plasmids // J. Microbiol. Biotechnol. 2012. V. 22. P. 1279–1287.</mixed-citation></citation-alternatives></ref><ref id="cit46"><label>46</label><citation-alternatives><mixed-citation xml:lang="ru">Suzuki H., Murakami A., Yoshida K.-I. Counterselection system for Geobacillus kaustophilus HTA426 through disruption of pyrF and pyrR // Appl. Envir. Microbiol. 2012. V. 78. P. 7376–7383.</mixed-citation><mixed-citation xml:lang="en">Suzuki H., Murakami A., Yoshida K.-I. Counterselection system for Geobacillus kaustophilus HTA426 through disruption of pyrF and pyrR // Appl. Envir. Microbiol. 2012. V. 78. P. 7376–7383.</mixed-citation></citation-alternatives></ref><ref id="cit47"><label>47</label><citation-alternatives><mixed-citation xml:lang="ru">Suzuki H., Yoshida K.-I., Ohshima T. Polysaccharide-degrading thermophiles generated by heterologous gene expres sion in Geobacillus kaustophilus HTA426 // Appl. Envir. Microbiol. 2013. V. 79. P. 5151–5158.</mixed-citation><mixed-citation xml:lang="en">Suzuki H., Yoshida K.-I., Ohshima T. Polysaccharide-degrading thermophiles generated by heterologous gene expres sion in Geobacillus kaustophilus HTA426 // Appl. Envir. Microbiol. 2013. V. 79. P. 5151–5158.</mixed-citation></citation-alternatives></ref><ref id="cit48"><label>48</label><citation-alternatives><mixed-citation xml:lang="ru">Takami H., Nishi S., Lu J. et al. Genomic characterization of thermophilic Geobacillus species isolated from the deepest sea mud of the Mariana Trench // Extremophiles. 2004a. V. 8. P. 351–356.</mixed-citation><mixed-citation xml:lang="en">Takami H., Nishi S., Lu J. et al. Genomic characterization of thermophilic Geobacillus species isolated from the deepest sea mud of the Mariana Trench // Extremophiles. 2004a. V. 8. P. 351–356.</mixed-citation></citation-alternatives></ref><ref id="cit49"><label>49</label><citation-alternatives><mixed-citation xml:lang="ru">Takami H., Takaki Y., Chee G.-J. et al. Thermoadaptation trait revealed by the genome sequence of thermophilic Geobacillus kaustophilus // Nucl. Acids Res. 2004b. V. 32. P. 6292–6303.</mixed-citation><mixed-citation xml:lang="en">Takami H., Takaki Y., Chee G.-J. et al. Thermoadaptation trait revealed by the genome sequence of thermophilic Geobacillus kaustophilus // Nucl. Acids Res. 2004b. V. 32. P. 6292–6303.</mixed-citation></citation-alternatives></ref><ref id="cit50"><label>50</label><citation-alternatives><mixed-citation xml:lang="ru">Talarico L.A., Gil M.A., Yomano L.P. et al. Construction and expression of an ethanol production operon in Gram-positive bacteria // Microbiol. 2005. V. 151. P. 4023–4031.</mixed-citation><mixed-citation xml:lang="en">Talarico L.A., Gil M.A., Yomano L.P. et al. Construction and expression of an ethanol production operon in Gram-positive bacteria // Microbiol. 2005. V. 151. P. 4023–4031.</mixed-citation></citation-alternatives></ref><ref id="cit51"><label>51</label><citation-alternatives><mixed-citation xml:lang="ru">Tang Y.J., Sapra R., Joyner D. et al. Analysis of metabolic pathways and fluxes in a newly discovered thermophilic and ethanol-tolerant Geobacillus strain // Biotechnol. Bioeng. 2009. V. 102. P. 1377–1386.</mixed-citation><mixed-citation xml:lang="en">Tang Y.J., Sapra R., Joyner D. et al. Analysis of metabolic pathways and fluxes in a newly discovered thermophilic and ethanol-tolerant Geobacillus strain // Biotechnol. Bioeng. 2009. V. 102. P. 1377–1386.</mixed-citation></citation-alternatives></ref><ref id="cit52"><label>52</label><citation-alternatives><mixed-citation xml:lang="ru">Taylor M.P., Esteban C.D., Leak D.J. Development of a versatile shuttle vector for gene expression in Geobacillus sрp. // Plasmid. 2008. V. 60. P. 45–52.</mixed-citation><mixed-citation xml:lang="en">Taylor M.P., Esteban C.D., Leak D.J. Development of a versatile shuttle vector for gene expression in Geobacillus sрp. // Plasmid. 2008. V. 60. P. 45–52.</mixed-citation></citation-alternatives></ref><ref id="cit53"><label>53</label><citation-alternatives><mixed-citation xml:lang="ru">Taylor M.P., Eley K.L., Martin S. et al. Thermophilic ethanologenesis: future prospects for second-generation bioethanol production // Trends Biotechnol. 2009. V. 27. P. 398–405.</mixed-citation><mixed-citation xml:lang="en">Taylor M.P., Eley K.L., Martin S. et al. Thermophilic ethanologenesis: future prospects for second-generation bioethanol production // Trends Biotechnol. 2009. V. 27. P. 398–405.</mixed-citation></citation-alternatives></ref><ref id="cit54"><label>54</label><citation-alternatives><mixed-citation xml:lang="ru">Thompson A.H., Studholme D.J., Green E.M., Leak D.J. Heterologous expression of pyruvate decarboxylase in Geobacillus thermoglucosidasius // Biotechnol. Lett. 2008. V. 30. P. 1359–1365.</mixed-citation><mixed-citation xml:lang="en">Thompson A.H., Studholme D.J., Green E.M., Leak D.J. Heterologous expression of pyruvate decarboxylase in Geobacillus thermoglucosidasius // Biotechnol. Lett. 2008. V. 30. P. 1359–1365.</mixed-citation></citation-alternatives></ref><ref id="cit55"><label>55</label><citation-alternatives><mixed-citation xml:lang="ru">Verhaart M.R.A., Bielen A.A.M., van der Oost J. et al. Hydrogen production by hyperthermophilic and extremely thermophilic bacteria and archaea: mechanisms for reductant disposal // Env. Technol. 2010. V. 31. P. 993–1003.</mixed-citation><mixed-citation xml:lang="en">Verhaart M.R.A., Bielen A.A.M., van der Oost J. et al. Hydrogen production by hyperthermophilic and extremely thermophilic bacteria and archaea: mechanisms for reductant disposal // Env. Technol. 2010. V. 31. P. 993–1003.</mixed-citation></citation-alternatives></ref><ref id="cit56"><label>56</label><citation-alternatives><mixed-citation xml:lang="ru">Verma D., Anand A., Satyanarayana T. Thermostable and alkalistable endoxylanase of the extremely thermophilic bacterium Geobacillus thermodenitrificans TSAA1: cloning, expression, characteristics and its applicability in generating xylooligosaccharides and fermentable sugars // Appl. Biochem. Biotechnol. 2013. V. 170. P. 119–130.</mixed-citation><mixed-citation xml:lang="en">Verma D., Anand A., Satyanarayana T. Thermostable and alkalistable endoxylanase of the extremely thermophilic bacterium Geobacillus thermodenitrificans TSAA1: cloning, expression, characteristics and its applicability in generating xylooligosaccharides and fermentable sugars // Appl. Biochem. Biotechnol. 2013. V. 170. P. 119–130.</mixed-citation></citation-alternatives></ref><ref id="cit57"><label>57</label><citation-alternatives><mixed-citation xml:lang="ru">Wagschal K., Heng C., Lee C.C. et al. Purification and characterization of a glycoside hydrolase family 43 beta-xylosidase from Geobacillus thermoleovorans IT-08 // Appl. Biochem. Biotechnol. 2009. V. 155. P. 304–313.</mixed-citation><mixed-citation xml:lang="en">Wagschal K., Heng C., Lee C.C. et al. Purification and characterization of a glycoside hydrolase family 43 beta-xylosidase from Geobacillus thermoleovorans IT-08 // Appl. Biochem. Biotechnol. 2009. V. 155. P. 304–313.</mixed-citation></citation-alternatives></ref><ref id="cit58"><label>58</label><citation-alternatives><mixed-citation xml:lang="ru">White D., Sharp R.J., Priest F.G. A polyphasic taxonomic study of thermophilic bacilli from a wide geographical area // Antonie van Leeuwenhoek. 1993. V. 64. P. 357–386.</mixed-citation><mixed-citation xml:lang="en">White D., Sharp R.J., Priest F.G. A polyphasic taxonomic study of thermophilic bacilli from a wide geographical area // Antonie van Leeuwenhoek. 1993. V. 64. P. 357–386.</mixed-citation></citation-alternatives></ref><ref id="cit59"><label>59</label><citation-alternatives><mixed-citation xml:lang="ru">Woese C.R., Fox G.E., Zablen L. et al. Conservation of primary structure in 16S ribosomal RNA // Nature. 1975. V. 254. P. 83–86.</mixed-citation><mixed-citation xml:lang="en">Woese C.R., Fox G.E., Zablen L. et al. Conservation of primary structure in 16S ribosomal RNA // Nature. 1975. V. 254. P. 83–86.</mixed-citation></citation-alternatives></ref><ref id="cit60"><label>60</label><citation-alternatives><mixed-citation xml:lang="ru">Wu L.J., Welker N.E. Protoplast transformation of Bacillus stearothermophilus NUB36 by plasmid DNA // J. General Microbiol. 1989. V. 135. P. 1315–1324.</mixed-citation><mixed-citation xml:lang="en">Wu L.J., Welker N.E. Protoplast transformation of Bacillus stearothermophilus NUB36 by plasmid DNA // J. General Microbiol. 1989. V. 135. P. 1315–1324.</mixed-citation></citation-alternatives></ref><ref id="cit61"><label>61</label><citation-alternatives><mixed-citation xml:lang="ru">Wu S., Liu B., Zhang X. Characterization of a recombinant thermostable xylanase from deep-sea thermophilic Geobacillus sp. MT-1 in East Pacific // Appl. Microbiol. Biotechnol. 2006. V. 72. P. 1210–1216.</mixed-citation><mixed-citation xml:lang="en">Wu S., Liu B., Zhang X. Characterization of a recombinant thermostable xylanase from deep-sea thermophilic Geobacillus sp. MT-1 in East Pacific // Appl. Microbiol. Biotechnol. 2006. V. 72. P. 1210–1216.</mixed-citation></citation-alternatives></ref><ref id="cit62"><label>62</label><citation-alternatives><mixed-citation xml:lang="ru">Xiao Z., Wang X., Huang Y. et al. Thermophilic fermentation of acetoin and 2,3-butanediol by a novel Geobacillus strain // Biotechnol. Biofuels. 2012. V. 5. P. 88.</mixed-citation><mixed-citation xml:lang="en">Xiao Z., Wang X., Huang Y. et al. Thermophilic fermentation of acetoin and 2,3-butanediol by a novel Geobacillus strain // Biotechnol. Biofuels. 2012. V. 5. P. 88.</mixed-citation></citation-alternatives></ref></ref-list><fn-group><fn fn-type="conflict"><p>The authors declare that there are no conflicts of interest present.</p></fn></fn-group></back></article>
