References

vavilov

Вавиловский журнал генетики и селекции

Vavilov Journal of Genetics and Breeding

2500-3259

Institute of Cytology and Genetics of Siberian Branch of the RAS

10.18699/VJ19.477

vavilov-1929

Research Article

ЭПИГЕНЕТИКА

EPIGENETICS

Функциональные свойства Su(Hw)-зависимого комплекса определяются его регуляторным окружением и множественными взаимодействиями на белковой платформе Su(Hw)

Functional properties of the Su(Hw) complex are determined by its regulatory environment and multiple interactions on the Su(Hw) protein platform

Мельникова

Л. С.

Melnikova

L. S.

Москва.

Moscow.

lsm73@mail.ru

Костюченко

М. В.

Kostyuchenko

M. V.

Москва.

Moscow.

Молодина

В. В.

Molodina

V. V.

Москва.

Moscow.

Георгиев

П. Г.

Georgiev

P. G.

Москва.

Moscow.

Головнин

А. К.

Golovnin

A. K.

Москва.

Moscow.

Институт биологии гена Российской академии наук.РоссияInstitute of Gene Biology, RAS.Russian Federation

2019

28032019

232168173

2019

Мельникова Л.С., Костюченко М.В., Молодина В.В., Георгиев П.Г., Головнин А.К.

Melnikova L.S., Kostyuchenko M.V., Molodina V.V., Georgiev P.G., Golovnin A.K.

This work is licensed under a Creative Commons Attribution 4.0 License.

https://vavilov.elpub.ru/jour/article/view/1929

Белок Su(Hw) был впервые идентифицирован как ДНК-связывающий компонент инсуляторного комплекса. Инсуляторы представляют собой регуляторные элементы, которые могут блокировать энхансер-промоторные взаимодействия и работать как границы между активным и репрессивным хроматином. Su(Hw)-зави симый белковый комплекс – уникальная модель для изучения инсуляторов, поскольку основные структурные компоненты, влияющие на его активность, уже известны. Однако механизмы, вовлекающие этот комплекс в различные регуляторные процессы, и детали взаимодействий между компонентами Su(Hw) инсуляторов остаются недостаточно изученными. Наши недавние работы выявили детальный механизм формирования и функционирования инсулятора Su(Hw). В представленном обзоре мы демонстрируем, как множественные взаимодействия между основными компонентами Su(Hw)-зависимого комплекса (Su(Hw)/ Mod(mdg4)-67.2/CP190) влияют на его активность. Показываем, что Su(Hw) может регулировать энхансерпромоторные взаимодействия через новый механизм нейтрализации инсулятора и, корме того, участвует в прямой регуляции активности близлежащих промоторов. Наконец, мы описываем механизм формирования «инсуляторных телец» и предлагаем модель, объясняющую их роль в рекрутировании Su(Hw)-зависимого комплекса на хроматин.

The Su(Hw) protein was first identified as a DNA-binding component of an insulator complex in Drosophila. Insulators are regulatory elements that can block the enhancer-promoter communication and exhibit boundary activity. Some insulator complexes contribute to the higher-order organization of chromatin in topologically associated domains that are fundamental elements of the eukaryotic genomic structure. The Su(Hw)-dependent protein complex is a unique model for studying the insulator, since its basic structural components affecting its activity are already known. However, the mechanisms involving this complex in various regulatory processes and the precise interaction between the components of the Su(Hw) insulators remain poorly understood. Our recent studies reveal the fine mechanism of formation and function of the Su(Hw) insulator. Our results provide, for the first time, an example of a high complexity of interactions between the insulator proteins that are required to form the (Su(Hw)/Mod(mdg4)-67.2/CP190) complex. All interactions between the proteins are to a greater or lesser extent redundant, which increases the reliability of the complex formation. We conclude that both association with CP190 and Mod(mdg4)-67.2 partners and the proper organization of the DNA binding site are essential for the efficient recruitment of the Su(Hw) complex to chromatin insulators. In this review, we demonstrate the role of multiple interactions between the major components of the Su(Hw) insulator complex (Su(Hw)/Mod(mdg4)-67.2/CP190) in its activity. It was shown that Su(Hw) may regulate the enhancer–promoter communication via the newly described insulator neutralization mechanism. Moreover, Su(Hw) participates in direct regulation of activity of vicinity promoters. Finally, we demonstrate the mechanism of organization of “insulator bodies” and suggest a model describing their role in proper binding of the Su(Hw) complex to chromatin.

Su(Hw)Mod(mdg4)-67.2CP190регуляция транскрипцииинсуляцияинсуляторные тельцабелок-белковые взаимодействия

Su(Hw)Mod(mdg4)-67.2CP190transcription regulationinsulationinsulator bodiesprotein-protein interactions.

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The authors declare that there are no conflicts of interest present.