vavilovВавиловский журнал генетики и селекцииVavilov Journal of Genetics and Breeding2500-3259Institute of Cytology and Genetics of Siberian Branch of the RAS10.18699/VJ19.478vavilov-1932Research ArticleЭПИГЕНЕТИКАEPIGENETICSРоль SAGA комплекса в транскрипции и экспорте мРНКThe role of SAGA in the transcription and export of mRNAhttps://orcid.org/0000-0002-7942-3924НабирочкинаЕ. Н.NabirochkinaE. N.

Москва.

Moscow.

elenan5@rambler.ruhttps://orcid.org/0000-0001-6772-6955КуршаковаМ. М.KurshakovaM. M.

Москва.

Moscow.

https://orcid.org/0000-0003-3665-0390ГеоргиеваС. Г.GeorgievaS. G.

Москва.

Moscow.

https://orcid.org/0000-0003-1086-6329КопытоваД. В.KopytovaD. V.

Москва.

Moscow.

Институт биологии гена Российской академии наук.РоссияInstitute of Gene Biology, RAS.Russian Federation201929032019232174179Copyright © Набирочкина Е.Н., Куршакова М.М., Георгиева С.Г., Копытова Д.В., 20192019Набирочкина Е.Н., Куршакова М.М., Георгиева С.Г., Копытова Д.В.Nabirochkina E.N., Kurshakova M.M., Georgieva S.G., Kopytova D.V.This work is licensed under a Creative Commons Attribution 4.0 License.https://vavilov.elpub.ru/jour/article/view/1932

Гистон-ацетил трансферазный комплекс SAGA/TFTC играет важную роль в регуляции транскрипции. Нами было обнаружено, что TFTC/STAGA комплексы метазоа деубиквитинируют гистоны H2Aи H2B. Был определен модуль TFTC/STAGA комплекса, который обладает деубиквитиназной активностью (DUBm). Мы исследовали DUBm у Drosophila melanogaster и идентифицировали гомологи – компоненты DUBm дрожжей. Нами было показано, что белок Sgf11, один из компонентов DUBm у Drosophila, обладает другой, отличной от DUBm функцией. Белок Sgf11 ассоциирован с CAP-содержащим комплексом и рекрутируется на растущую матричную рибонуклеиновую кислоту (мРНК). Кроме того, мы обнаружили, что Sgf11 взаимодействует с TREX-2/AMEX комплексом экспорта мРНК, и этот белок необходим для экспорта мРНК из ядра. Другие две субъединицы DUBm Drosophila также обладают функциями, отличными от функции DUBm. Так, выявлено, что Sus1/ENY2 присутствует в нескольких различных комплексах. Эксперименты по иммуноокрашиванию политенных хромосом личинок Drosophila показали, что Sgf11 присутствует на всех сайтах локализации генов, кодирующих мяРНК, и что так же, как hSNAPC1, dPbp45, субъединица PBP комплекса, играющая ключевую роль в транскрипции мяРНК, присутствует не только в сайтах генов мяРНК, но и в других сайтах активной транскрипции, осуществляемой РНКполимеразой II (PolII). Мы провели колокализацию на политенных хромосомах белков Sgf11 и Brf1 (компонента комплекса РНК-полимеразы III) и обнаружили, что Sgf11 находится во многих сайтах активной транскрипции и присутствует в тех же сайтах, что Brf1. Таким образом, мы показали, что SAGA коактивирует транскрипцию как РНК-полимеразы II-зависимых, так и РНК-полимеразы III-зависимых генов малых ядерных РНК.

SAGA/TFTC, which is a histone acetyltransferase complex, plays an important role in the regulation of transcription. We have identified that the metazoan TFTC/STAGA complexes had histone H2A and H2B deubiquitinase activity that is carried out by a DUBm (deubiquitination module). We studied the DUBm of SAGA in Drosophila melanogaster and identified Drosophila homologs of yeast DUBm components. Two subunits of DUBm (Sus1/ENY2 and Sgf11) were shown to have functions separate from DUBm function. Thus, Sus1/ENY2 was shown to be present in several different complexes. Sgf11 was found to be associated with the cap-binding complex (CBC) and recruited onto growing messenger ribonucleic acid (mRNA). Also, we have shown that Sgf11 interacted with the TREX-2/AMEX mRNA export complex and was essential for mRNA export from the nucleus. Immunostaining of the polytene chromosomes of Drosophila larvae revealed that Sgf11 is present at the sites of localization of snRNA genes. It was also found in immunostaining experiments that dPbp45, the subunit of the PBP complex, the key player in the snRNA transcription process, is associated not only with the snRNA gene localization sites, but with other sites of active transcription by PolII. We also revealed that Sgf11 was present at many active transcription sites in interbands and puffs on polytene chromosomes, Sgf11 was localized at all Brf1 (the component of the RNA polymerase III basal transcription complex) sites. We concluded that SAGA coactivated transcription of both the PolII and PolIII-dependent snRNA genes.

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The authors declare that there are no conflicts of interest present.