vavilovВавиловский журнал генетики и селекцииVavilov Journal of Genetics and Breeding2500-3259Institute of Cytology and Genetics of Siberian Branch of the RASvavilov-198Research ArticleСтатьиArticlesИССЛЕДОВАНИЕ ВОСПРОИЗВОДИМОСТИ РЕЗУЛЬТАТОВ ИДЕНТИФИКАЦИИ МИКРООРГАНИЗМОВ С ПОМОЩЬЮ метода МАЛДИ ВРЕМЯПРОЛЕТНОЙ МАСС-СПЕКТРОМЕТРИИ В ЗАВИСИМОСТИ ОТ УСЛОВИЙ КУЛЬТИВИРОВАНИЯ НА ПРИМЕРЕ Geobacillus stearothermоphilusREPRODUCIBILITY OF THE RESULTS OF MICROBE IDENTIFICATION BY MALDI-TOF MASS SPECTROMETRY DEPENDING ON GROWTH CONDITIONS BY THE EXAMPLE OF Geobacillus stearothermоphilusСтаростинК. В.StarostinK. V.starostin@bionet.nsc.ruДемидовЕ. А.DemidovE. A.starostin@bionet.nsc.ruРозановА. С.RozanovA. S.starostin@bionet.nsc.ruБрянскаяА. В.BryanskayaA. V.starostin@bionet.nsc.ruПельтекС. Е.PeltekS. E.starostin@bionet.nsc.ruФедеральное государственное бюджетное научное учреждение «Федеральный исследовательский центр Институт цитологии и генетики Сибирского отделения Российской академии наук», Новосибирск, РоссияРоссияInstitute of Cytology and Genetics SB RAS, Novosibirsk, RussiaRussian Federation201311012015174/1748757Copyright © Старостин К.В., Демидов Е.А., Розанов А.С., Брянская А.В., Пельтек С.Е., 20152015Старостин К.В., Демидов Е.А., Розанов А.С., Брянская А.В., Пельтек С.Е.Starostin K.V., Demidov E.A., Rozanov A.S., Bryanskaya A.V., Peltek S.E.This work is licensed under a Creative Commons Attribution 4.0 License.https://vavilov.elpub.ru/jour/article/view/198

Потребность в быстрых и точных методах идентификации микроорганизмов является насущной проблемой для самых различных сфер человеческой деятельности. Прежде всего это, конечно, касается клинической диагностики, но данные методы востребованы также в экологическом мониторинге, фармацевтической и пищевой промышленности, научных исследованиях и т. д. На сегодняшний день существует множество методов идентификации микроорганизмов (фенотипические, генотипические, хемотаксономические методы, прямое белковое профилирование и др.). В данной работе авторы оценивают влияние различных условий культивирования, таких как температура, время роста и тип питательной среды, на воспроизводимость результатов идентификации микроорганизмов методом МАЛДИ времяпролетной масс-спектрометрии на примере трех штаммов Geobacillus stearothermоphilus.

Rapid and accurate methods of microorganism identification are essential in various human activities. They include primarily clinical diagnostics. In addition, they are in demand in environment monitoring, harmacology, food industry, research, etc. There are diverse approaches to microbe identification: phenotyping, genotyping, chemotaxonomy, direct protein profiling, etc. In this work, the effects of various growth conditions, such as temperature, growth time, and nutrition medium, on the reproducibility of microbe identification by MALDI-TOF mass spectrometry are considered by the example of three Geobacillus stearothermоphilus strains.

МАЛДИпрямое белковое профилированиеидентификация микроорганизмовGeobacillus stearothermоphilusMALDIdirect protein profilingmicroorganism identificationGeobacillus stearothermоphilusгрант № 14.512.11.0057 Министерства образования и науки Российской ФедерацииReferencesBöhme K., Fernández-No I.C., Pazos M. et al. Identification and classification of seafood-borne pathogenic and spoilage bacteria: 16S rRNA sequencing versus MALDI-TOF MS fingerprinting // Electrophoresis. 2013. V. 34. No. 6. P. 877–887.Böhme K., Fernández-No I.C., Pazos M. et al. Identification and classification of seafood-borne pathogenic and spoilage bacteria: 16S rRNA sequencing versus MALDI-TOF MS fingerprinting // Electrophoresis. 2013. V. 34. No. 6. P. 877–887.Cripps R.E., Elay K., Leak D.J. et al. Metabolic engineering of Geobacillus thermoglucosidasius for high yield ethanol production // Metab. Eng. 2009. V. 11. No. 6. P. 398–408.Cripps R.E., Elay K., Leak D.J. et al. Metabolic engineering of Geobacillus thermoglucosidasius for high yield ethanol production // Metab. Eng. 2009. V. 11. No. 6. P. 398–408.Dickinson D.N., La Duc M.T., Satomi M. et al. MALDI-TOFMS compared with other polyphasic taxonomy approaches for the identification and classification of Bacillus pumilus spores // J. Microbiol. Meth. 2004. V. 58. No. 1. P. 1–12.Dickinson D.N., La Duc M.T., Satomi M. et al. MALDI-TOFMS compared with other polyphasic taxonomy approaches for the identification and classification of Bacillus pumilus spores // J. Microbiol. Meth. 2004. V. 58. No. 1. P. 1–12.Freiwald A., Sauer S. Phylogenetic classification and identification of bacteria by mass spectrometry // Nat. Protoc. 2009. V. 4. No. 5. P. 732–742.Freiwald A., Sauer S. Phylogenetic classification and identification of bacteria by mass spectrometry // Nat. Protoc. 2009. V. 4. No. 5. P. 732–742.Mellmann A., Cloud J., Maier T. et al. Evaluation of matrixassisted laser desorption ionization-time-of-flight mass spectrometry in comparison to 16S rRNA gene sequencing for species identification of nonfermenting bacteria // J. Clin. Microbiol. 2008. V. 46. No. 6. P. 1946–54.Mellmann A., Cloud J., Maier T. et al. Evaluation of matrixassisted laser desorption ionization-time-of-flight mass spectrometry in comparison to 16S rRNA gene sequencing for species identification of nonfermenting bacteria // J. Clin. Microbiol. 2008. V. 46. No. 6. P. 1946–54.Nazina T.N., Tourova T.P., Poltaraus A.B. et al. Taxonomic study of aerobic thermophilic bacilli: descriptions of Geobacillus subterraneus gen. nov., sp. nov. and Geobacillus uzenensis sp. nov. from petroleum reservoirs and transfer of Bacillus stearothermophilus, Bacillus thermocatenulatus, Bacillus thermoleovorans, Bacillus kaustophilus, Bacillus thermoglucosidasius and Bacillus thermodenitrificans to Geobacillus as the new combinations G. stearothermophilus, G. thermocatenulatus, G. thermoleovorans, G. kaustophilus, G. thermoglucosidasius and G. thermodenitrificans // Intern. J. Syst. Evol. Microbiol. 2001. V. 51. No. 2. P. 433–446.Nazina T.N., Tourova T.P., Poltaraus A.B. et al. Taxonomic study of aerobic thermophilic bacilli: descriptions of Geobacillus subterraneus gen. nov., sp. nov. and Geobacillus uzenensis sp. nov. from petroleum reservoirs and transfer of Bacillus stearothermophilus, Bacillus thermocatenulatus, Bacillus thermoleovorans, Bacillus kaustophilus, Bacillus thermoglucosidasius and Bacillus thermodenitrificans to Geobacillus as the new combinations G. stearothermophilus, G. thermocatenulatus, G. thermoleovorans, G. kaustophilus, G. thermoglucosidasius and G. thermodenitrificans // Intern. J. Syst. Evol. Microbiol. 2001. V. 51. No. 2. P. 433–446.Ruelle V., El Moualij B., Zorzi W. et al. Rapid identification of environmental bacterial strains by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry // Rapid Commun. in Mass Spectrometry: RCM. 2004. V. 18. No. 18. P. 2013–9.Ruelle V., El Moualij B., Zorzi W. et al. Rapid identification of environmental bacterial strains by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry // Rapid Commun. in Mass Spectrometry: RCM. 2004. V. 18. No. 18. P. 2013–9.Ryzhov V., Fenselau C. Characterization of the protein subset desorbed by MALDI from whole bacterial cells // Analyt. Chem. 2001. V. 73. No. 4. P. 746–50.Ryzhov V., Fenselau C. Characterization of the protein subset desorbed by MALDI from whole bacterial cells // Analyt. Chem. 2001. V. 73. No. 4. P. 746–50.Valentine N., Wunschel D., Petersen C., Wahl K. Effect of culture conditions on microorganism identification by matrix-assisted laser desorption ionization mass spectrometry // Appl. Environmental Microbiol. 2005. V. 71. No. 1. P. 58–64.Valentine N., Wunschel D., Petersen C., Wahl K. Effect of culture conditions on microorganism identification by matrix-assisted laser desorption ionization mass spectrometry // Appl. Environmental Microbiol. 2005. V. 71. No. 1. P. 58–64.

The authors declare that there are no conflicts of interest present.