vavilovВавиловский журнал генетики и селекцииVavilov Journal of Genetics and Breeding2500-3259Institute of Cytology and Genetics of Siberian Branch of the RASvavilov-199Research ArticleСтатьиArticlesПРИМЕНЕНИЕ МАЛДИ ВРЕМЯПРОЛЕТНОЙ МАСС-СПЕКТРОМЕТРИИ ДЛЯ ИДЕНТИФИКАЦИИ МИКРООРГАНИЗМОВMALDI-TOF MASS SPECTROMETRY IN MICROORGANISM IDENTIFICATIONДемидовЕ. А.DemidovE. A.scratch_nsu@ngs.ruСтаростинК. В.StarostinK. V.scratch_nsu@ngs.ruПопикВ. М.PopikV. M.scratch_nsu@ngs.ruПельтекС. Е.PeltekS. E.scratch_nsu@ngs.ruФедеральное государственное бюджетное научное учреждение «Федеральный исследовательский центр Институт цитологии и генетики Сибирского отделения Российской академии наук», Новосибирск, РоссияРоссияInstitute of Cytology and Genetics SB RAS, Novosibirsk, RussiaRussian FederationФедеральное государственное бюджетное учреждение науки Институт ядерной физики им. Г.И. Будкеpа Сибирского отделения Российской академии наукРоссияInstitute of Cytology and Genetics SB RAS, Novosibirsk, RussiaRussian Federation201311012015174/1758764Copyright © Демидов Е.А., Старостин К.В., Попик В.М., Пельтек С.Е., 20152015Демидов Е.А., Старостин К.В., Попик В.М., Пельтек С.Е.Demidov E.A., Starostin K.V., Popik V.M., Peltek S.E.This work is licensed under a Creative Commons Attribution 4.0 License.https://vavilov.elpub.ru/jour/article/view/199

В последнее десятилетие наряду с классическими и молекулярно-биологическими методами идентификации микроорганизмов, все чаще применяется метод идентификации микроорганизмов по их белковым профилям, или прямое белковое профилирование. Данный метод не уступает по таким показателям, как точность и специфичность идентификации, однако его выгодно отличают быстрота проведения и более низкая себестоимость анализов. В данном обзоре приведены современные представления о возможностях данного метода, а также дано сравнение с используемыми методами идентификации микроорганизмов.

Identification of microorganisms from their protein profiles, or direct protein profiling, has been extensively used in the last decade. Being sufficiently accurate and specific, this method is fast and inexpensive. In this review, current notions of the potential of this method are considered and it is compared with other approaches to microorganism identification.

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The authors declare that there are no conflicts of interest present.