vavilovВавиловский журнал генетики и селекцииVavilov Journal of Genetics and Breeding2500-3259Institute of Cytology and Genetics of Siberian Branch of the RAS10.18699/VJ19.493vavilov-2020Research ArticleГЕНЕТИКА РАСТЕНИЙPLANT GENETICSПолиморфизм ITS-последовательностей генов 35S рРНК у видов Elymus dahuricus aggr.: два криптических вида?Polymorphism of ITS sequences in 35S rRNA genes in Elymus dahuricus aggregate species: two cryptic species?https://orcid.org/0000-0003-1146-1622РодионовА. В.RodionovA. V.

биологический факультет

Faculty of Biology

avrodionov@mail.ruhttps://orcid.org/0000-0002-5468-6883ДобряковаК. С.DobryakovaK. S.https://orcid.org/0000-0002-7096-9242НосовН. Н.NosovN. N.https://orcid.org/0000-0002-5264-5594ГнутиковА. А.GnutikovA. A.https://orcid.org/0000-0001-9745-4604ПунинаЕ. О.PuninaE. O.https://orcid.org/0000-0002-8715-6723КрюковА. А.KriukovA. A.https://orcid.org/0000-0002-6122-6770ШнеерВ. С.ShneyerV. S.Ботанический институт им. В.Л. Комарова Российской академии наук; Санкт-Петербургский государственный университетРоссияKomarov Botanical Institute, RAS; Saint-Petersburg State UniversityRussian FederationБотанический институт им. В.Л. Комарова Российской академии наукРоссияKomarov Botanical Institute, RASRussian FederationБотанический институт им. В.Л. Комарова Российской академии наук; Федеральный исследовательский центр Всероссийский институт генетических ресурсов растений им. Н.И. Вавилова (ВИР)РоссияKomarov Botanical Institute, RAS; Federal Research Center the N.I. Vavilov All-Russian Institute of Plant Genetic Resources (VIR)Russian FederationВсероссийский научно-исследовательский институт сельскохозяйственной микробиологииРоссияAll-Russia Research Institute for Agricultural MicrobiologyRussian Federation201914052019233287295Copyright © Родионов А.В., Добрякова К.С., Носов Н.Н., Гнутиков А.А., Пунина Е.О., Крюков А.А., Шнеер В.С., 20192019Родионов А.В., Добрякова К.С., Носов Н.Н., Гнутиков А.А., Пунина Е.О., Крюков А.А., Шнеер В.С.Rodionov A.V., Dobryakova K.S., Nosov N.N., Gnutikov A.A., Punina E.O., Kriukov A.A., Shneyer V.S.This work is licensed under a Creative Commons Attribution 4.0 License.https://vavilov.elpub.ru/jour/article/view/2020

Секвенированы последовательности внутренних транскрибируемых спейсеров (ITS) 23 видов и подвидов Elymus sensu lato. При анализе молекулярно-филогенетической сети Neighbor-Net все последовательности ITS образцов Elymus s. l. были разделены на четыре типа: основной северный Elymus-риботип, основной южный Elymus-риботип, северный dahuricus-риботип и южный dahuricus-риботип. рДНК основного южного риботипа родственна рДНК диплоидного вида Pseudoroegneria stipifolia (PI 313960) и P. spicata (PI 547161). рДНК основного северного риботипа родственна рДНК P. cognata (PI 531720), диплоидного вида из Казахстана, несущего StY – вариант St-генома. Основной северный риботип широко распространен у видов Elymus Сибири и Дальнего Востока, включая Якутию и Чукотку. Основной южный St-риботип характерен для относительно южных популяций Elymus и Pseudoroegneria, включая Закавказье, Приморье, Пакистан, Южную Корею. Отметим, что северный Elymus dahuricus-риботип и южный Elymus dahuricus-риботип были обнаружены у всех четырех видов группы родства E. dahuricus aggr.: E. dahuricus Turcz. ex Griseb., E. franchetii Kitag., E. excelsus Turcz. ex Griseb. и у гималайского вида E. tangutorum (Nevski) Hand.-Mazz. Иными словами, молекулярно-филогенетические исследования образцов, относимых к E. dahuricus aggr., говорят о том, что в Сибири, на Дальнем Востоке и в Северном Китае существуют по крайней мере две группы популяций (две расы), надежно различающиеся по ITS-последовательностям, в каждой из которых представлены все морфологические формы, относимые сейчас одними систематиками к четырем разным видам E. dahuricus aggr. (E. dahuricus sensu stricto, E. franchetii, E. tangutorum, E. excelsus), а другими – к одному виду Campeiostachys dahurica (Turcz. ex Griseb.) B.R. Baum, J.L. Yang et C.C. Yen. Имеются ли между этими группами морфологические различия, или это криптические виды (подвиды) – неизвестно, но с уверенностью можно сказать, что если различия в морфологии между этими двумя расами есть, то они не связаны с признаками, которые сейчас считаются таксономически значимыми и используются для разделения E. dahuricus s. s., E. franchetii, E. excelsus, E. tangutorum.

Nuclear ribosomal internal transcribed spacer (ITS) sequences were sequenced for 23 species and subspecies of Elymus sensu lato collected in Russia. The Neighbor-Net analysis of ITS sequences suggested that there are four ribotypes called Core Northern St-rDNA, Core Southern St-rDNA, Northern dahuricus St-rDNA and Southern dahuricus St-rDNA. The Core Southern variant of St-rDNA is closely related to rDNA of diploid Pseudoroegneria stipifolia (PI 313960) and P. spicata (PI 547161). The Core Northern St-rDNA is closely related to rDNA of P. cognata (PI 531720), a diploid species of Kyrgyzstan carrying StY variant of the St genome. The Core Northern St-rDNA is widespread among the Elymus species of Siberia and the Far East, including Yakutia and Chukotka. The Core Southern St-ribotype is typical of southern Elymus and Pseudoroegneria of the South Caucasus, Primorye, Pakistan, and South Korea. The Northern dahuricus St-ribotype and Southern dahuricus St-ribotype are derivatives of the Core Northern and Core Southern St-ribotypes, correspondingly. Both of them were found in all four studied species of the E. dahuricus aggregate: E. dahuricus Turcz. ex Griseb., E. franchetii Kitag., E. excelsus Turcz. ex Griseb. and Himalayan E. tangutorum (Nevski) Hand.-Mazz. In other words, there are at least two population groups (two races) of the Elymus dahuricus aggregate species that consistently differ in their ITS-sequences in Siberia, the Far East and Northern China. Each contains all morphological forms, which taxonomists now attribute either to different species of E. dahuricus aggr. (E. dahuricus sensu stricto, E. franchetii, E. tangutorum, E. excelsus) or subspecies of Campeiostachys dahurica (Turcz. ex Griseb.) B.R. Baum, J.L. Yang et C.C. Yen. At the moment it is unknown if there are any morphological differences between plants carrying either Northern or Southern dahuricus rDNA. Probably, they are cryptic species, but it is certain that if differences in morphology between the two races exist, they are not associated with signs that are now considered taxonomically significant and are used to separate E. dahuricus s. s., E. franchetii, E. tangutorum, and E. excelsus.

Elymus dahuricus aggr.межвидовая гибридизация35S рРНКTriticeaeElymus dahuricus aggr.interspecific hybridizationrDNA35S rRNATriticeaeWe thank N.N. Tzvelev of the Komarov Botanical Institute and N.S. Probatova of the Federal Scientific Center of the East Asia Terrestrial Biodiversity, Far Eastern Branch of the Russian Academy of Sciences for their interest to the work and expert help in the taxonomic identification of Elymus species. The authors also would like to acknowledge the funding by the Russian Foundation for Basic Research Projects No. 15-04-06438 and 18-04-01040 as well as the Ministry of Education and Sciences of the Russian Federation (АААА-А18-118040290161-3, 0126-2016-0001, BIN RAS). The research was done using equipment of The Core Facilities Center “Cell and Molecular Technologies in Plant Science” at the Komarov Botanical Institute RAS (St. Petersburg, Russia).We thank N.N. Tzvelev of the Komarov Botanical Institute and N.S. 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The authors declare that there are no conflicts of interest present.