References

vavilov

Вавиловский журнал генетики и селекции

Vavilov Journal of Genetics and Breeding

2500-3259

Institute of Cytology and Genetics of Siberian Branch of the RAS

10.18699/VJ19.494

vavilov-2021

Research Article

Селекция растений на иммунитет и продуктивность

Plant breeding for immunity and performance

Молекулярная идентификация генов устойчивости к стеблевой ржавчине в интрогрессивных линиях яровой мягкой пшеницы

Molecular identification of the stem rust resistance genes in the introgression lines of spring bread wheat

https://orcid.org/0000-0001-9439-2102

Баранова

О. А.

Baranova

O. A.

baranova_oa@mail.ru

https://orcid.org/0000-0001-8324-9765

Сибикеев

С. Н.

Sibikeev

S. N.

https://orcid.org/0000-0002-3968-2470

Дружин

А. Е.

Druzhin

A. E.

Всероссийский научно-исследовательский институт защиты растенийРоссияAll-Russian Institute of Plant ProtectionRussian Federation

Научно-исследовательский институт сельского хозяйства Юго-ВостокаРоссияAgricultural Research Institute of the South-East RegionRussian Federation

2019

14052019

233296303

2019

Баранова О.А., Сибикеев С.Н., Дружин А.Е.

Baranova O.A., Sibikeev S.N., Druzhin A.E.

This work is licensed under a Creative Commons Attribution 4.0 License.

https://vavilov.elpub.ru/jour/article/view/2021

Проанализированы 57 интрогрессивных линий и 11 сортов яровой мягкой пшеницы селекции НИИ сельского хозяйства Юго-Востока, возделываемых на территории Поволжья. Линии получены с участием синтетиков селекции CIMMYT, сортов твердой пшеницы, прямого скрещивания с Agropyron elongatum (CI-7-57) и имеют интрогрессии от родственных видов мягкой пшеницы, а именно транслокации от Ag. elongatum (7DS-7DL7Ae#1L), Aegilops speltoides (2D-2S), Ae. ventricosum (2AL-2AS-2MV#1), ржи культурной (1BL-1R#1S), замещения от Ag. intermedium 6Agi (6D) и тритикале Satu. Сорта и линии были оценены на устойчивость к саратовской, лысогорской, дербентской и омской популяциям возбудителя стеблевой ржавчины, а также проанализированы на наличие идентифицированных Sr генов устойчивости с использованием известных молекулярных маркеров. Анализ устойчивости сортов и линий к саратовской популяции патогена в полевых условиях, а также к омской, дербентской и лысогорской популяциям Puccinia graminis f. sp. tritici на стадии проростков показал потерю эффективности генов Sr25 и Sr6Agi . Ген Sr31 пока сохраняет свою эффективность. Ко всем взятым в анализ популяциям патогена была устойчива 31 линия пшеницы из 57 (54.4 % образцов). У исследуемых интрогрессивных линий идентифицированы гены Sr31/Lr26, Sr25/Lr19, Sr28, Sr57/Lr34 и Sr38/Lr37. Ген Sr31/Lr26 определен у 19 линий (33.3 % образцов). Все линии, несущие транслокацию 1RS.1BL (Sr31/Lr26), были устойчивы ко всем взятым в анализ популяциям патогена. Ген Sr25/Lr19 идентифицирован у 49 линий (86 % образцов). Сочетание генов Sr31/Lr26+Sr25/Lr19 идентифицировано у 15 линий (26.3 %). У одной линии идентифицировано сочетание генов Sr38/Lr37+Sr25/Lr19, у другой линии – сочетание генов Sr57/Lr34+ +Sr25/Lr19, и еще у одной – Sr31/Lr26+Sr25/Lr19+Sr28. Все они были устойчивы ко всем взятым в анализ популяциям патогена. Гены Sr2, Sr24, Sr26, Sr32, Sr36, Sr39 у анализируемых линий обнаружены не были.

A total of 57 introgression lines and 11 cultivars of spring bread wheat developed by All-Russian Institute of Plant Protection and cultivated in the Volga Region were analyzed. The lines were obtained with the participation of CIMMYT synthetics, durum wheat cultivars, direct crossing with Agropyron elongatum (CI-7-57) and have introgressions from related species of bread wheat, namely translocations from Ag. elongatum (7DS-7DL-7Ae#1L), Aegilops speltoides (2D-2S), Ae. ventricosum (2AL-2AS-2MV#1), Secale cereale (1BL-1R#1S), 6Agi (6D) substitution from Ag. intermedium and triticale Satu. Cultivars and lines were assessed for resistance to Saratov, Lysogorsk, Derbent and Omsk stem rust pathogen populations (Puccinia graminis f. sp. tritici), and analyzed for the presence of the known Sr resistance genes using molecular markers. The analysis of the cultivars’ and lines’ resistance to the Saratov pathogen population in the field, as well as to Omsk, Derbent and Lysogorsk populations at the seedling stage, showed the loss of efficiency of the Sr25 and Sr6Agi genes. The Sr31 gene remained effective. Thirty one wheat lines out of 57 (54.4 % of samples) were resistant to all pathogen populations taken into analysis. The Sr31/Lr26, Sr25/Lr19, Sr28, Sr57/Lr34 and Sr38/Lr37 genes were identified in the introgression lines. The Sr31/Lr26 gene was identified in 19 lines (33.3 % of samples). All lines carrying the 1RS.1BL translocation (Sr31/Lr26) were resistant to all pathogen populations taken into analysis. The Sr25/Lr19 gene was identified in 49 lines (86 %). The gene combination Sr31/Lr26+ Sr25/Lr19 was identified in 15 lines (26.3 %). The gene combinations Sr38/Lr37+Sr25/Lr19, Sr57/Lr34+Sr25/Lr19 and Sr31/Lr26+Sr25/Lr19+Sr28 were identified in 3 introgression lines. These three lines were characterized by resistance to the pathogen populations studied in this work. The Sr2, Sr24, Sr26, Sr32, Sr36 and Sr39 genes were not detected in the analyzed wheat lines.

яровая мягкая пшеницаинтрогрессивные линииPuccinia graminis f. sp. triticiгены Sr.

spring bread wheatintrogression linesPuccinia graminis f. sp. triticiSr genes

This work was supported by the RFBR grant No. 18-016-00170a.

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The authors declare that there are no conflicts of interest present.