References

vavilov

Вавиловский журнал генетики и селекции

Vavilov Journal of Genetics and Breeding

2500-3259

Institute of Cytology and Genetics of Siberian Branch of the RAS

10.18699/VJ19.563

vavilov-2341

Research Article

Устойчивость растений к биотическим факторам

Идентификация локусов количественных признаков устойчивости к листовой и стеблевой ржавчинам мягкой пшеницы (Triticum aestivum L.) с использованием картирующей популяции Памяти Азиева × Парагон

Identification of QTLs for resistance to leaf and stem rusts in bread wheat (Triticum aestivum L.) using a mapping population of ‘Pamyati Azieva × Paragon’

https://orcid.org/0000-0001-5987-2952

Гениевская

Ю. А.

Genievskaya

Алматы.

Almaty.

Федоренко

Е. Н.

Fedorenko

Шагалалы.

Shagalaly.

Сарбаев

А. Т.

Sarbayev

Алмалыбак.

Almalybak.

https://orcid.org/0000-0002-7903-3467

Амалова

А. Ы.

Amalova

Алматы.

Almaty.

https://orcid.org/0000-0002-9748-507X

Абугалиева

С. И.

Abugalieva

Алматы.

Almaty.

Гриффис

С.

Griffiths

Норидж.

Norwich.

https://orcid.org/0000-0001-8590-1745

Туруспеков

Е. К.

Turuspekov

Алматы.

Almaty.

yerlant@yahoo.com

Институт биологии и биотехнологии растенийКазахстанInstitute of Plant Biology and BiotechnologyKazakhstan

Северо-Казахстанская сельскохозяйственная опытная станцияКазахстанNorth-Kazakhstan Agricultural StationKazakhstan

Казахский НИИ земледелия и растениеводстваКазахстанKazakh Research Institute of Agriculture and Plant IndustryKazakhstan

Институт биологии и биотехнологии растений; Казахский Национальный университет им. аль-Фараби, факультет биологии и биотехнологии, кафедра биоразнообразия и биоресурсовКазахстанInstitute of Plant Biology and Biotechnology; al-Farabi Kazakh National University, Faculty of Biology and Biotechnology, Department of Biodiversity and BioresourcesKazakhstan

Центр им. Джона ИннесаВеликобританияJohn Innes CentreUnited Kingdom

2019

23112019

237887895

2019

Гениевская Ю.А., Федоренко Е.Н., Сарбаев А.Т., Амалова А.Ы., Абугалиева С.И., Гриффис С., Туруспеков Е.К.

Genievskaya Y., Fedorenko Y., Sarbayev A., Amalova A., Abugalieva S., Griffiths S., Turuspekov Y.

This work is licensed under a Creative Commons Attribution 4.0 License.

https://vavilov.elpub.ru/jour/article/view/2341

Листовая и стеблевая ржавчины являются вредоносными грибными болезнями мягкой пшеницы (Triticum aestivum L.). Целью данного исследования была идентификация локусов количественных признаков (ЛКП), связанных с устойчивостью к болезням, в двух регионах возделывания пшеницы в Казахстане. Для этого картирующая популяция Памяти Азиева × Парагон, состоящая из 98 рекомбинантно-инбредных линий и выращиваемая на севере и юго-востоке Казахстана, была фенотипирована по степени поражения листовой и стеблевой ржавчиной и проанализирована по ключевым компонентам урожайности. Выявлена отрицательная корреляция между степенью поражения болезнью и продуктивностью растений в обоих регионах. Картирующая популяция была генотипирована с использованием ДНК микрочипа Illumina на 20 000 маркеров ОНП (однонуклеотидный полиморфизм). Для дальнейшего анализа отобрано 4 595 полиморфных маркеров ОНП. Для идентификации ЛКП, связанных с устойчивостью картирующей популяции к листовой и стеблевой ржавчинам, использовалась программа Windows QTL Cartographer v2.5. В северном регионе были обнаружены два ЛКП устойчивости к листовой ржавчине и восемь ЛКП – к стеблевой. Локализации восьми из них совпали с позициями известных генов Lr и Sr. Два ЛКП для стеблевой ржавчины были обозначены как новые. Для юго-восточного региона обнаружено восемь ЛКП, ассоциированных с устойчивостью к листовой ржавчине, и один – к стеблевой. Настоящее исследование является первым шагом в генетическом картировании локусов устойчивости к листовой и стеблевой ржавчинам мягкой пшеницы в Казахстане. Полевые исследования в двух регионах страны и генотипирование выбранной картирующей популяции позволили выявить ключевые ЛКП, которые будут эффективны в региональных селекционных проектах, направленных на улучшение продуктивности мягкой пшеницы.

Leaf rust (LR) and stem rust (SR) are harmful fungal diseases of bread wheat (Triticum aestivum L.). The purpose of this study was to identify QTLs for resistance to LR and SR that are effective in two wheat-growing regions of Kazakhstan. To accomplish this task, a population of recombinant inbred lines (RILs) of ‘Pamyati Azieva × Paragon’ was grown in the northern and southeastern parts of Kazakhstan, phenotyped for LR/SR severities, and analyzed for key yield components. The study revealed a negative correlation between disease severity and plant productivity in both areas. The mapping population was genotyped using a 20,000 Illumina SNP array. A total of 4595 polymorphic SNP markers were further selected for linkage analysis after filtering based on missing data percentage and segregation distortion. Windows QTL Cartographer was applied to identify QTLs associated with LR and SR resistances in the RIL mapping population studied. Two QTLs for LR resistance and eight for SR resistance were found in the north, and the genetic positions of eight of them have matched the positions of the known Lr and Sr genes, while two QTLs for SR were novel. In the southeast, eight QTLs for LR and one for SR were identified in total. The study is an initial step of the genetic mapping of LR and SR resistance loci of bread wheat in Kazakhstan. Field trials in two areas of the country and the genotyping of the selected mapping population have allowed identification of key QTLs that will be effective in regional breeding projects for better bread wheat productivity.

мягкая пшеницакартированиерекомбинантно-инбредные линиилокусы количественных признаковлистовая ржавчинастеблевая ржавчина

linkage mappingrecombinant inbred linesqualitative trait locileaf ruststem rust

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The authors declare that there are no conflicts of interest present.