vavilovВавиловский журнал генетики и селекцииVavilov Journal of Genetics and Breeding2500-3259Institute of Cytology and Genetics of Siberian Branch of the RAS10.18699/VJ19.564vavilov-2342Research ArticleУстойчивость растений к биотическим факторамСемейство генов дегидрогеназ коричного спирта вовлечено в ответ устойчивых и восприимчивых генотипов льна на заражение Fusarium oxysporumThe cinnamyl alcohol dehydrogenase gene family is involved in the response to Fusarium oxysporum in resistant and susceptible flax genotypesНоваковскийР. О.NovakovskiyR. O.

Москва.

Moscow.

ПовховаА. В.PovkhovaL. V.

Москва.

Долгопрудный.

Moscow.

Dolgoprudny.

КрасновГ. С.KrasnovG. S.

Москва.

 

Moscow.

РожминаТ. А.RozhminaT. A.

Москва.

Торжок.

Moscow.

Torzhok.

ЖученкоА. А.ZhuchenkoA. A.

Торжок.

Москва.

Torzhok.

Moscow.

КудрявцеваЛ. П.KudryavtsevaL. P.

Торжок.

Torzhok.

ПушковаЕ. Н.PushkovaE. N.

Москва.

Moscow.

КезиманаП.KezimanaP.

Москва.

Moscow.

КудрявцеваА. В.KudryavtsevaA. V.

Москва.

Moscow.

ДмитриевА. А.DmitrievA. A.

Москва.

Moscow.

МельниковаН. В.MelnikovaN. V.

Москва.

Moscow.

mnv-4529264@yandex.ruИнститут молекулярной биологии им. В.А. Энгельгардта Российской академии наукРоссияEngelhardt Institute of Molecular Biology, Russian Academy of Sciences,Russian FederationИнститут молекулярной биологии им. В.А. Энгельгардта Российской академии наук; Московский физико-технический институт (Национальный исследовательский университет)РоссияEngelhardt Institute of Molecular Biology, Russian Academy of Sciences; Moscow Institute of Physics and TechnologyRussian FederationИнститут молекулярной биологии им. В.А. Энгельгардта Российской академии наукРоссияEngelhardt Institute of Molecular Biology, Russian Academy of SciencesRussian FederationИнститут молекулярной биологии им. В.А. Энгельгардта Российской академии наук; Федеральный научный центр лубяных культурРоссияEngelhardt Institute of Molecular Biology, Russian Academy of Sciences; Federal Research Center for Bast Fiber CropsRussian FederationФедеральный научный центр лубяных культур; Всероссийский селекционно-технологический институт садоводства и питомниководстваРоссияFederal Research Center for Bast Fiber Crops; All-Russian Horticultural Institute for Breeding, Agrotechnology and NurseryRussian FederationФедеральный научный центр лубяных культурРоссияFederal Research Center for Bast Fiber CropsRussian FederationИнститут молекулярной биологии им. В.А. Энгельгардта Российской академии наук; Российский университет дружбы народовРоссияEngelhardt Institute of Molecular Biology, Russian Academy of Sciences; Peoples’ Friendship University of Russia (RUDN University)Russian Federation201923112019237896901Copyright © Новаковский Р.О., Повхова А.В., Краснов Г.С., Рожмина Т.А., Жученко А.А., Кудрявцева Л.П., Пушкова Е.Н., Кезимана П., Кудрявцева А.В., Дмитриев А.А., Мельникова Н.В., 20192019Новаковский Р.О., Повхова А.В., Краснов Г.С., Рожмина Т.А., Жученко А.А., Кудрявцева Л.П., Пушкова Е.Н., Кезимана П., Кудрявцева А.В., Дмитриев А.А., Мельникова Н.В.Novakovskiy R.O., Povkhova L.V., Krasnov G.S., Rozhmina T.A., Zhuchenko A.A., Kudryavtseva L.P., Pushkova E.N., Kezimana P., Kudryavtseva A.V., Dmitriev A.A., Melnikova N.V.This work is licensed under a Creative Commons Attribution 4.0 License.https://vavilov.elpub.ru/jour/article/view/2342

Лен (Linum usitatissimum L.) используется для производства текстиля, масел, фармацевтических препаратов и композитных материалов. Крайне вредоносным заболеванием, снижающим урожайность льна, является фузариозное увядание, вызываемое грибом Fusarium oxysporum f. sp. lini. Созданы устойчивые к фузариозному увяданию сорта льна и определены гены, вовлеченные в ответ на F. oxysporum, однако механизмы устойчивости L. usitatissimum к этому патогену до сих пор неясны. В настоящем исследовании мы использовали данные секвенирования транскриптомов восприимчивых и устойчивых генотипов льна, выращенных в контрольных условиях или зараженных F. oxysporum. Проанализировано около 250 миллионов прочтений, полученных на секвенаторе NextSeq Illumina. После фильтрации прочтений для исключения транскриптома F. oxysporum оставшиеся прочтения картировали на геном L. usitatissimum и провели их количественный анализ. Оценили экспрессию генов семейства CAD, которые, как известно, участвуют в ответе на заражение F. oxysporum, у устойчивых и восприимчивых к фузариозному увяданию генотипов. Изменение экспрессии в ответ на возбудителя выявили для всех 13 исследованных генов CAD. Наиболее значительные различия в экспрессии между контрольными и инфицированными растениями наблюдались для генов CAD1B, CAD4A, CAD5A и CAD5B: сильное повышение экспрессии выявлено для CAD1B, CAD5A и CAD5B, а сильное снижение – для CAD4A. Для большинства генов CAD уровни экспрессии были близкими при одинаковых условиях вы- ращивания для всех изученных генотипов льна. Статистически значимое различие в изменении экспрессии между группами устойчивых и восприимчивых генотипов выявлено только для гена CAD1A. Наше исследование указывает на активное участие генов CAD в ответе растений льна на F. oxysporum, но не приводит свидетельств их роли в качестве кандидатов в гены устойчивости. Полученные результаты вносят вклад в понимание механизмов ответа льна на заражение F. oxysporum и роли генов CAD в устойчивости к стрессовым воздействиям.

Flax (Linum usitatissimum L.) is used for the production of textile, oils, pharmaceuticals, and composite materials. Fusarium wilt, caused by the fungus Fusarium oxysporum f. sp. lini, is a very harmful disease that reduces flax production. Flax cultivars that are resistant to Fusarium wilt have been developed, and the genes that are involved in the host response to F. oxysporum have been identified. However, the mechanisms underlying resistance to this pathogen remain unclear. In the present study, we used transcriptome sequencing data obtained from susceptible and resistant flax genotypes grown under control conditions or F. oxysporum infection. Approximately 250 million reads, generated with an Illumina NextSeq instrument, were analyzed. After filtering to exclude the F. oxysporum transcriptome, the remaining reads were mapped to the L. usitatissimum genome and quantified. Then, the expression levels of cinnamyl alcohol dehydrogenase (CAD) family genes, which are known to be involved in the response to F. oxysporum, were evaluated in resistant and susceptible flax genotypes. Expression alterations in response to the pathogen were detected for all 13 examined CAD genes. The most significant differences in expression between control and infected plants were observed for CAD1B, CAD4A, CAD5A, and CAD5B, with strong upregulation of CAD1B, CAD5A, and CAD5B and strong downregulation of CAD4A. When plants were grown under the same conditions, the expression levels were similar in all studied flax genotypes for most CAD genes, and statistically significant differences in expression between resistant and susceptible genotypes were only observed for CAD1A. Our study indicates the strong involvement of CAD genes in flax response to F. oxysporum but brings no evidence of their role as resistance gene candidates. These findings contribute to the understanding of the mechanisms underlying the response of flax to F. oxysporum infection and the role of CAD genes in stress resistance.

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The authors declare that there are no conflicts of interest present.