vavilovВавиловский журнал генетики и селекцииVavilov Journal of Genetics and Breeding2500-3259Institute of Cytology and Genetics of Siberian Branch of the RAS10.18699/VJ19.566vavilov-2344Research ArticleГенетические ресурсы растенийИзучение линий мягкой пшеницы селекции Национального центра зерна им. П.П. Лукьяненко по аллельным вариантам генов WaxyAllelic variants for Waxy genes in common wheat lines bred at the Lukyanenko National Grain CenterДавоянЭ. Р.DavoyanE. R.

Краснодар.

Krasnodar.

davayan@rambler.ruhttps://orcid.org/0000-0003-3844-9682БеспаловаЛ. А.BespalovaL. A.

Краснодар.

Krasnodar.

ДавоянР. О.DavoyanR. O.

Краснодар.

Krasnodar.

АгаеваЕ. В.AgaevaE. V.

Краснодар.

Krasnodar.

БукрееваГ. И.BukreevaG. I.

Краснодар.

Krasnodar.

ЗубановаЮ. С.ZubanovaYu. S.

Краснодар.

Krasnodar.

МиковД. С.MikovD. S.

Краснодар.

Krasnodar.

БолдаковД. М.BoldakovD. M.

Краснодар.

Krasnodar.

Национальный центр зерна им. П.П. ЛукьяненкоРоссияNational Center of Grain named after P.P. LukyanenkoRussian Federation201923112019237910915Copyright © Давоян Э.Р., Беспалова Л.А., Давоян Р.О., Агаева Е.В., Букреева Г.И., Зубанова Ю.С., Миков Д.С., Болдаков Д.М., 20192019Давоян Э.Р., Беспалова Л.А., Давоян Р.О., Агаева Е.В., Букреева Г.И., Зубанова Ю.С., Миков Д.С., Болдаков Д.М.Davoyan E.R., Bespalova L.A., Davoyan R.O., Agaeva E.V., Bukreeva G.I., Zubanova Y.S., Mikov D.S., Boldakov D.M.This work is licensed under a Creative Commons Attribution 4.0 License.https://vavilov.elpub.ru/jour/article/view/2344

В статье представлены результаты изучения с помощью молекулярных маркеров линий мягкой пшеницы (Triticum aestivum L.) по аллельным вариантам генов Wx. Исследование проводили в рамках работ по передаче нуль-аллелей генов Wx-А1, Wx-В1, Wx-D1 в сорта мягкой пшеницы и созданию селекционного материала с измененной активностью основных ферментов, участвующих в биосинтезе амилозы. Линии получены в отделе селекции и семеноводства пшеницы и тритикале Национального центра зерна им. П.П. Лукьяненко в результате скрещивания мутантных форм – носителей неактивных (нуль-аллелей) генов Wx-А1, Wx-В1, Wx- D1 с коммерческими сортами мягкой пшеницы. Отобранные для работы молекулярные маркеры поволили выявить ценный селекционный материал, имеющий в своем геноме как единичные нуль-аллели генов Wx, так и их комбинации. Сочетание двух нуль-аллелей (Wx-A1b + Wx-D1b) обнаружено у 30 линий. Наличие трех нуль-аллелей (Wx-A1b + Wx-B1b + Wx-D1b), что соответствует полностью Wx-пшеницам, обнаружено у одной линии. Отобрано 37 линий, сочетающих присутствие аллеля Wx-B1e с нуль-аллелями Wx-A1b и Wx-D1b. У 26 линий идентифицирована комбинация (Wx-A1b + Wx-B1e), 24 линии имели сочетание алле- лей (Wx-B1e + Wx- D1b). Идентифицировано, что мутантные формы PI619381, PI619384, PI619386 – носители функционального аллеля Wx-B1e. Аллели Wx-А1b и Wx-B1e могли быть переданы в изучаемые линии как от используемых доноров, так и от сортов Старшина и Коротышка соответственно. Донорами привнесения в линии аллелей Wx-B1b и Wx-D1b служат использованные в скрещиваниях мутантные формы. Применение отобранных нами молекулярных маркеров для идентификации аллельного состояния генов Wx-А1, Wx-В1, Wx-D1 может стать эффективной основой для селекции с помощью молекулярных маркеров (MAS) по дан- ному признаку. Отобранные линии с идентифицированными в них нуль-аллелями генов Wx представляют интерес для селекционных программ, направленных на улучшение технологических качеств зерна и полу- чение сортов мягкой пшеницы с новыми свойствами крахмала.

This article presents the results of a molecular marker-assisted study of allelic variants of Wx genes in common wheat (Triticum aestivum L.) lines. The study was carried out as part of the work on the transfer of null alleles of the genes Wx-A1, Wx-B1, and Wx-D1 to the varieties of soft wheat and creation of breeding material with modified activities of the main enzymes involved in amylose biosynthesis. The lines were obtained at the Department of Breeding and Seed Production of Wheat and Triticale, National Center of Grain named after P.P. Lukyanenko, by crossing mutant forms carrying inactive (null) alleles of genes Wx-A1, Wx-B1, and Wx-D1 with bread wheat cultivars. The molecular markers selected for the study allowed identification of valuable breeding material carrying both single null alleles of Wx genes and their combinations in its genome. A combination of two null alleles (Wx-A1b + Wx-D1b) was detected in 30 lines. The presence of three null alleles (Wx-A1b + Wx-B1b + Wx-D1b), which corresponded to fully Wx wheat, was found in one line. We selected 37 lines that combined the presence of the Wx-B1e allele with the Wx-A1b and Wx-D1b null alleles. The Wx-A1b + Wx-B1e combination was identified in 26 lines, and 24 lines carried the combination of alleles Wx-B1e + Wx-D1b. The mutant forms PI619381, PI619384, and PI619386 were identified as carriers of the functional Wx-B1e allele. The Wx-A1b and Wx-B1e alleles could have been transferred to the studied lines from the donors used or from the Starshina and Korotyshka varieties, respectively. The mutant forms used in the crosses are donors of the Wx-B1b and Wx-D1b alleles. The use of molecular markers chosen by us for identification of the allelic state of the Wx-A1, Wx-B1, and Wx-D1 genes can provide grounds for marker-assisted selection for this trait. Selected lines found to possess null alleles of the Wx genes are applicable in breeding programs aimed at the improvement of technological qualities of grain and raise of bread wheat varieties with modified starch properties.

мягкая пшеницамолекулярные маркерыгены Wxcommon wheatmolecular markersWx genesReferencesChao S., Sharp P.J., Worland A.J., Warham E.J., Koebner R.M.D., Gale M.D. RFLP-based genetic maps of wheat homoeologous group 7 chromosomes. Theor. Appl. Genet. 1989;78(4):495-504.Chao S., Sharp P.J., Worland A.J., Warham E.J., Koebner R.M.D., Gale M.D. RFLP-based genetic maps of wheat homoeologous group 7 chromosomes. Theor. Appl. Genet. 1989;78(4):495-504.Divashuk M.G., Klimushina M.V., Karlov G.I. Molecular genetic characteristics of the Wx-B1e allele from common wheat and applicability of the DNA markers for its identification. Russ. J. Genet. 2011; 47(12):1428-1432.Divashuk M.G., Klimushina M.V., Karlov G.I. Molecular genetic characteristics of the Wx-B1e allele from common wheat and applicability of the DNA markers for its identification. Russ. J. Genet. 2011; 47(12):1428-1432.Hayakawa K., Tanaka K., Nakamura T., Endo S., Hoshino T. End use quality of waxy wheat flour in various grain-based foods. Cereal Chem. 2004;81(5):666-672.Hayakawa K., Tanaka K., Nakamura T., Endo S., Hoshino T. End use quality of waxy wheat flour in various grain-based foods. Cereal Chem. 2004;81(5):666-672.Kiribuchi-Otobe C., Nagamine T., Yanagisawa T., Phnishi M., Yamaguchi I. Production of hexaploid wheats with waxy endosperm character. Cereal Chem. 1997;74:72-74.Kiribuchi-Otobe C., Nagamine T., Yanagisawa T., Phnishi M., Yamaguchi I. Production of hexaploid wheats with waxy endosperm character. Cereal Chem. 1997;74:72-74.Klimushina M.V., Gladkih N.I., Divashuk M.G., Bespalova L.A., Vasilyev A.V., Karlov G.I. Distribution of allelic variants of Wx genes in the common wheat collection made at the Krasnodar Lukyanenko Research Institute of Agriculture. Vavilovskii Zhurnal Genetiki i Selektsii = Vavilov Journal of Genetics and Breeding. 2012; 16(1):187-192. (in Russian)Klimushina M.V., Gladkih N.I., Divashuk M.G., Bespalova L.A., Vasilyev A.V., Karlov G.I. Distribution of allelic variants of Wx genes in the common wheat collection made at the Krasnodar Lukyanenko Research Institute of Agriculture. Vavilovskii Zhurnal Genetiki i Selektsii = Vavilov Journal of Genetics and Breeding. 2012; 16(1):187-192. (in Russian)Maningat C.C., Seib P.A. Update on wheat starch and its uses. In: Proc. Int. Wheat Quality Conf. J.L. Steele, O.K. Chung (Eds.). Manhattan, KS.: Grain Industry Alliance, 1997;261-284.Maningat C.C., Seib P.A. Update on wheat starch and its uses. In: Proc. Int. Wheat Quality Conf. J.L. Steele, O.K. Chung (Eds.). Manhattan, KS.: Grain Industry Alliance, 1997;261-284.Maningat C.C., Seib P.A., Bassi S.D., Woo K.S., Lasater G.D. Wheat starch: Production, properties, modification, and uses. In: BeMiller J., Whistler R. (Eds.). Starch Chemistry and Technology. New York: Academic Press, 2009;441-510.Maningat C.C., Seib P.A., Bassi S.D., Woo K.S., Lasater G.D. Wheat starch: Production, properties, modification, and uses. In: BeMiller J., Whistler R. (Eds.). Starch Chemistry and Technology. New York: Academic Press, 2009;441-510.McLauchlan A., Ogbonnaya F.C., Hollingsworth B. Development of robust PCR-based DNA markers for each homeoallele of granulebond starch synthase and their application in wheat breeding programs. Aust. J. Agric. Res. 2001;52:1409-1416.McLauchlan A., Ogbonnaya F.C., Hollingsworth B. Development of robust PCR-based DNA markers for each homeoallele of granulebond starch synthase and their application in wheat breeding programs. Aust. J. Agric. Res. 2001;52:1409-1416.Nakamura T., Jamamori M., Hirano H., Hidaka S. Production of waxy (amylase-free) wheat. Mol. Gen. Genet. 1995;248:253-259.Nakamura T., Jamamori M., Hirano H., Hidaka S. Production of waxy (amylase-free) wheat. Mol. Gen. Genet. 1995;248:253-259.Nakamura T., Vrinten P., Saito M., Konda M. Rapid classification of partial waxy wheats using PCR-based markers. Genome. 2002;45: 1150-1156.Nakamura T., Vrinten P., Saito M., Konda M. Rapid classification of partial waxy wheats using PCR-based markers. Genome. 2002;45: 1150-1156.Oda M., Yasuda Y., Okazaki S., Yamauchi Y., Yokoyama Y. A method of flour quality assessment for Japanese noodles. Cereal Chem. 1980; 57:253-259.Oda M., Yasuda Y., Okazaki S., Yamauchi Y., Yokoyama Y. A method of flour quality assessment for Japanese noodles. Cereal Chem. 1980; 57:253-259.Plaschke J., Ganal M.W., Röder M.S. Detection of genetic diversity in closely related bread wheat using microsatellite markers. Theor. Appl. Genet. 1995;91:1001-1007.Plaschke J., Ganal M.W., Röder M.S. Detection of genetic diversity in closely related bread wheat using microsatellite markers. Theor. Appl. Genet. 1995;91:1001-1007.Saito M., Vrinten P., Ishikawa G., Graybosch R., Nakamura T. A novel codominant marker for selection of the null Wx-B1 allele in wheat breeding programs. Mol. Breed. 2009;23:209-217.Saito M., Vrinten P., Ishikawa G., Graybosch R., Nakamura T. A novel codominant marker for selection of the null Wx-B1 allele in wheat breeding programs. Mol. Breed. 2009;23:209-217.Shariflou M.R., Hassani M.E., Sharp P.J. A PCR-based DNA marker for detection of mutant and normal alleles of the Wx-D1 gene of wheat. Plant Breed. 2001;120(2):121-124.Shariflou M.R., Hassani M.E., Sharp P.J. A PCR-based DNA marker for detection of mutant and normal alleles of the Wx-D1 gene of wheat. Plant Breed. 2001;120(2):121-124.Shure M., Wessler S., Fedoroff N. Molecular identification and isolation of the Waxy locus in maize. Cell. 1983;35(1):225-233.Shure M., Wessler S., Fedoroff N. Molecular identification and isolation of the Waxy locus in maize. Cell. 1983;35(1):225-233.Toyokawa H., Rubenthaler G.L., Powers J.R., Schanus E.G. Japanese noodle qualities. 2. Starch components. Cereal Chem. 1989;66:387-391.Toyokawa H., Rubenthaler G.L., Powers J.R., Schanus E.G. Japanese noodle qualities. 2. Starch components. Cereal Chem. 1989;66:387-391.Vanzetti L.S., Pflüger L.A., Rodrίguez-Quijano M., Carrillo J.M., Helguera M. Genetic variability for waxy genes in Argentinean bread wheat germplasm. Electronic J. Biotechnol. 2009;12:1-9.Vanzetti L.S., Pflüger L.A., Rodrίguez-Quijano M., Carrillo J.M., Helguera M. Genetic variability for waxy genes in Argentinean bread wheat germplasm. Electronic J. Biotechnol. 2009;12:1-9.Yamamori M., Nakamura T., Endo T.R., Nagamine T. Waxy protein deficiency and chromosomal location of coding genes in common wheat. Theor. Appl. Genet. 1994;89:179-184.Yamamori M., Nakamura T., Endo T.R., Nagamine T. Waxy protein deficiency and chromosomal location of coding genes in common wheat. Theor. Appl. Genet. 1994;89:179-184.Zeng M., Morris C.F., Batey I.I., Wrigley C.W. Sources of variation for starch gelatinization, pasting, and gelation properties in wheat. CerealZeng M., Morris C.F., Batey I.I., Wrigley C.W. Sources of variation for starch gelatinization, pasting, and gelation properties in wheat. CerealChem. 1997;74:63-71.Chem. 1997;74:63-71.

The authors declare that there are no conflicts of interest present.