vavilovВавиловский журнал генетики и селекцииVavilov Journal of Genetics and Breeding2500-3259Institute of Cytology and Genetics of Siberian Branch of the RAS10.18699/VJ19.577vavilov-2394Research ArticleГЕНЕТИКА И СЕЛЕКЦИЯ РАСТЕНИЙ НА ИММУНИТЕТ, КАЧЕСТВО, ПРОДУКТИВНОСТЬ И СТРЕССОУСТОЙЧИВОСТЬANIMAL GENETICS AND BREEDINGВлияние экзогенного хорионического гонадотропина человека на овуляцию у мышейEffect of exogenous human chorionic gonadotropin on ovulation in miceАмстиславскийС. Я.AmstislavskyS. Ya.

Новосибирск

Novosibirsk

amstis@bionet.nsc.ruРанневаС. В.RannevaS. V.

Новосибирск

Novosibirsk

РагаеваД. С.RagaevaD. S.

Новосибирск

Novosibirsk

ЧуйкоЭ. А.ChuykoE. A.

Новосибирск

Novosibirsk

ПопковаА. М.PopkovaA. M.

Новосибирск

Novosibirsk

БрусенцевЕ. Ю.BrusentsevE. Yu.

Новосибирск

Novosibirsk

Федеральный исследовательский центр Институт цитологии и генетики Сибирского отделения Российской академии наук; Новосибирский национальный исследовательский государственный университетРоссияInstitute of Cytology and Genetics, SB RAS; Novosibirsk State UniversityRussian FederationФедеральный исследовательский центр Институт цитологии и генетики Сибирского отделения Российской академии наукРоссияInstitute of Cytology and Genetics, SB RASRussian FederationНовосибирский национальный исследовательский государственный университетРоссияNovosibirsk State UniversityRussian Federation20190901202023810061010Copyright © Амстиславский С.Я., Раннева С.В., Рагаева Д.С., Чуйко Э.А., Попкова А.М., Брусенцев Е.Ю., 20202020Амстиславский С.Я., Раннева С.В., Рагаева Д.С., Чуйко Э.А., Попкова А.М., Брусенцев Е.Ю.Amstislavsky S.Y., Ranneva S.V., Ragaeva D.S., Chuyko E.A., Popkova A.M., Brusentsev E.Y.This work is licensed under a Creative Commons Attribution 4.0 License.https://vavilov.elpub.ru/jour/article/view/2394

Применение некоторых вспомогательных репродуктивных технологий (ВРТ), в частности гормональной стимуляции овуляции, может привести к изменению числа и качества получаемых ооцитов. Целью исследования было изучение характера овуляции у мышей линии CD1 после воздействия на самок хорионическим гонадотропином человека (ХГЧ) и исследование последствий этого воздействия на длительность беременности, эмбриональные потери и вес рожденных потомков. При воздействии препаратом ХГЧ не было обнаружено достоверных различий по общему числу овулировавших ооцитов по сравнению с контрольными самками, а также различий по числу незрелых (без полярного тела) и зрелых (с формирующимся полярным телом) форм овулировавших ооцитов. Число же ооцитов с полярным телом у мышей экспериментальной группы после гормональной стимуляции овуляции препаратом ХГЧ было достоверно больше (p < 0.05) по сравнению с контрольной группой (6.2 ± 0.86 и 2.2 ± 0.9 соответственно). Не было получено достоверных различий между группами по продолжительности беременности и числу рожденных потомков, включая долю живо- и мертворожденных особей. Однако вес детенышей на пятый день после рождения в экспериментальной группе был достоверно меньше (p < 0.001), чем в контроле (3.16 ± 0.09 и 3.76 ± 0.07 соответственно). Таким образом, введение экзогенного ХГЧ самкам мышей стимулирует развитие ооцитов in vivo, что приводит к формированию большего числа их зрелых форм, но потомки, рожденные самками, стимулированными препаратом ХГЧ, имеют меньший вес тела в первые дни после рождения.

The implementation of assisted reproductive technologies (ART), hormonal stimulation in particular, may change the quality of ovulated oocytes. The purpose of our work was to study ovulation in CD1 mice after their stimulation with human chorionic gonadotropin (hCG) and to investigate the effects of such hormonal stimulation on the pregnancy duration, fetal losses and the weight of the offspring. No significant differences were found in the total number of ovulated oocytes or in the number of immature (without a polar body) ovulated oocytes; nor were there differences between the groups in the number of oocytes with a developing polar body. However, the number of matured oocytes with a distinct polar body was significantly higher (p < 0.05) in mice stimulated with hCG (experimental group) as compared with the controls (6.2 ± 0.86 and 2.2 ± 0.97, respectively). No significant differences were observed between the experimental and control mice in the duration of pregnancy or in the numbers of term offspring, including the percentage of live and stillborn pups. However, the body weight of the offspring in the experimental group was significantly lower (p < 0.001) as compared with the controls on the fifth day after birth (3.16 ± 0.09 and 3.76 ± 0.07, respectively). Thus, exogenous hCG facilitates the development of mouse oocytes in vivo, which leads to the larger number of their mature forms at ovulation, however, the offspring born after hCG-stimulated pregnancy was characterized by a lower body weight on the fifth day after birth.

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The authors declare that there are no conflicts of interest present.