vavilovВавиловский журнал генетики и селекцииVavilov Journal of Genetics and Breeding2500-3259Institute of Cytology and Genetics of Siberian Branch of the RASvavilov-240Research ArticleСтатьиArticlesКОМПЬЮТЕРНОЕ ИССЛЕДОВАНИЕ РЕГУЛЯЦИИ ТРАНСКРИПЦИИ ГЕНОВ ЭУКАРИОТ С ПОМОЩЬЮ ДАННЫХ ЭКСПЕРИМЕНТОВ СЕКВЕНИРОВАНИЯ И ИММУНОПРЕЦИПИТАЦИИ ХРОМАТИНАCOMPUTER-ASSISTED STUDY OF THE REGULATION OF EUKARYOTIC GENE TRANSCRIPTION ON THE BASE OF DATA ON CHROMATIN SEQUENCING AND PRECIPITATIONОрловЮ. Л.OrlovYu. L.orlov@bionet.nsc.ruФедеральное государственное бюджетное научное учреждение «Федеральный исследовательский центр Институт цитологии и генетики Сибирского отделения Российской академии наук», Новосибирск, РоссияРоссияInstitute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, RussiaRussian Federation201413012015181193206Copyright © Орлов Ю.Л., 20152015Орлов Ю.Л.Orlov Y.L.This work is licensed under a Creative Commons Attribution 4.0 License.https://vavilov.elpub.ru/jour/article/view/240

Появление высокопроизводительных экспериментальных технологий секвенирования привело к бурному росту объема данных о структуре генома, распределении регуляторных районов генов в геноме, особенностях их взаимодействия.

Статья представляет обзор технологий, связанных с иммунопреципитацией хроматина: ChIP-PET, ChIP-seq, ChIA-PET. Описаны компьютерные методы анализа сайтов связывания транскрипционных факторов и структуры регуляторных районов в масштабе генома. Показаны подходы к решению задач, возникающих при аннотации геномных данных, определении сайтов связывания транскрипционных факторов и регуляторных районов.

The development of high-throughput sequencing technologies dramatically accelerated the accumulation of data on genome structure, distribution of gene regulatory regions in the genome and specific features of their interaction. Technologies associated with chromatin immunoprecipitation are reviewed: ChIP-PET, ChIP-seq, and ChIA-PET. Computer-assisted methods for the analysis of transcription factor binding sites (TFBSs) and regulatory region structures throughout the genome are described. The paper demonstrates approaches to the solution of tasks related to genomic data annotation and identification of TFBSs and regulatory regions.

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The authors declare that there are no conflicts of interest present.