vavilovВавиловский журнал генетики и селекцииVavilov Journal of Genetics and Breeding2500-3259Institute of Cytology and Genetics of Siberian Branch of the RAS10.18699/VJ19.580vavilov-2400Research ArticleБИОТЕХНОЛОГИЯ В ПОСТГЕНОМНУЮ ЭРУMEDICAL GENETICSАссоциация длины теломер лейкоцитов и уровня специфических антител после вакцинации против клещевого энцефалитаAssociation between leukocyte telomere length and specific antibody levels after vaccination against tick-borne encephalitishttps://orcid.org/0000-0002-1947-5554ЮдинН. С.YudinN. S.

Новосибирск

Novosibirsk

yudin@bionet.nsc.ruБелявскаяВ. А.BelyavskayaV. A.

р. п. Кольцово, Новосибирская область

Koltsovo, Novosibirsk region

МаксимовВ. Н.MaksimovV. N.

Новосибирск

Novosibirsk

https://orcid.org/0000-0002-0403-545XИванощукД. Е.IvanoshchukD. E.

Новосибирск

Novosibirsk

ОрловП. С.OrlovP. S.

Новосибирск

Novosibirsk

https://orcid.org/0000-0001-9425-413XВоеводаМ. И.VoevodaM. I.

Новосибирск

Novosibirsk

Федеральный исследовательский центр Институт цитологии и генетики Сибирского отделения Российской академии наук; Новосибирский национальный исследовательский государственный университет; Научно-исследовательский институт терапии и профилактической медициныРоссияInstitute of Cytology and Genetics, SB RAS; Novosibirsk State University; Research Institute of Internal and Preventive MedicineRussian FederationГосударственный научный центр вирусологии и биотехнологии «Вектор» Федеральной службы по надзору в сфере защиты прав потребителей и благополучия человекаРоссияState Research Center of Virology and Biotechnology “Vector” of the Federal Service for Surveillance in Consumer Rights Protection and Human Well-beingRussian Federation20190901202023810261031Copyright © Юдин Н.С., Белявская В.А., Максимов В.Н., Иванощук Д.Е., Орлов П.С., Воевода М.И., 20202020Юдин Н.С., Белявская В.А., Максимов В.Н., Иванощук Д.Е., Орлов П.С., Воевода М.И.Yudin N.S., Belyavskaya V.A., Maksimov V.N., Ivanoshchuk D.E., Orlov P.S., Voevoda M.I.This work is licensed under a Creative Commons Attribution 4.0 License.https://vavilov.elpub.ru/jour/article/view/2400

Основным принципом индивидуализации вакцинаций является создание достаточной иммунной защиты организма при избегании излишней иммунизации. Отсюда возникает необходимость разработки методов прогнозирования величины иммунного ответа еще до проведения вакцинации. Длину теломер можно рассматривать как перспективный прогностический параметр для оценки иммунной реакции пациента при проведении вакцинации. Целью работы был анализ возможной ассоциации длины теломер лейкоцитов с уровнем специфических антител после вакцинации против клещевого энцефалита. В исследование было включено 55 мужчин и 40 женщин, ранее не вакцинированных против клещевого энцефалита и не имевших контактов с клещами. Вакцинацию проводили вакциной «ЭнцеВир». Через месяц после вакцинации анализировали уровень специфических антител IgG против вируса клещевого энцефалита с использованием тест-системы «ВектоВКЭ-IgG-стрип», а также длину теломер лейкоцитов с помощью количественной ПЦР в реальном времени. По уровню ответа на вакцинацию пациентов разделяли на нереагировавших (уровень IgG 0–100 МЕ/мл), низкореагировавших (уровень IgG 101–200 МЕ/мл) и высокореагировавших (уровень IgG выше 200 МЕ/мл). Длина теломер как минимум на уровне тенденции (p < 0.1) зависела как от ответа на вакцинацию, так и от возраста, уровня образования и наличия психоэмоционального стресса. С помощью общей линейной модели была выявлена ассоциация длины теломер с ответом на вакцинацию против клещевого энцефалита на уровне тенденции (p < 0.1) только у женщин. При попарном сравнении длина теломер у высокореагировавших женщин была достоверно выше, чем у нереагировавших. Таким образом, нами обнаружена ассоциация длины теломер лейкоцитов и уровня специфических антител после вакцинации против клещевого энцефалита у женщин. По-видимому, длину теломер лейкоцитов в крови можно рассматривать в качестве перспективного маркера для прогноза пролиферативного ответа лимфоцитов и величины иммунологической реакции пациента в ответ на вакцинацию.

The primary objective of personalized vaccination is to induce an efficient immune defense while avoiding excessive immunization. Hence, it necessitates the development of methods for predicting the magnitude of the immune response prior to vaccination. Telomere length can be considered as a promising prognostic parameter for assessing the immune response to vaccination. The aim of the work was to analyze the possible association between leukocyte telomere length and specific antibody levels after vaccination against tick-borne encephalitis. The study included 55 men and 40 women who had not previously been vaccinated against tick-borne encephalitis and had no contacts with ticks. Vaccination was carried out with the EnceVir vaccine. One month after vaccination, the level of specific IgG antibodies against tick-borne encephalitis virus was analyzed using the VektoVKE-IgG-strip test system and leukocyte telomere length was measured using real-time quantitative PCR. According to the intensity of vaccine-elicited immune responses, patients were divided into three groups: unresponsive (IgG level 0–100 IU/ml), slightly responsive (IgG level 101–200 IU/ml) and highly responsive (IgG level above 200 IU/ml). The telomere length, at least at trend level (p < 0.1), correlated with the response to vaccination as well as age, educational level and the presence of emotional stress. Using a general linear model, an association between telomere length and immune response to vaccination against tick-borne encephalitis at trend level (p < 0.1) was found only in women. Using a pairwise comparison, it was found that telomere length was significantly higher in highly responsive women than in unresponsive women. Hence, an association between leukocyte telomere length and specific antibody levels after vaccination against tick-borne encephalitis was identified in women. Therefore, peripheral blood leukocyte telomere length can be considered as a promising marker for predicting lymphocyte proliferative responses and the magnitude of vaccine-elicited cellular immune responses.

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The authors declare that there are no conflicts of interest present.