vavilovВавиловский журнал генетики и селекцииVavilov Journal of Genetics and Breeding2500-3259Institute of Cytology and Genetics of Siberian Branch of the RAS10.18699/VJ19.581vavilov-2401Research ArticleЭкологическая генетикаEcological geneticsРазнообразие mariner-подобных элементов OrthopteraDiversity of mariner-like elements in Orthopterahttps://orcid.org/0000-0003-4346-3868УстьянцевК. В.UstyantsevK.

Новосибирск

Novosibirsk

ustyantsev@bionet.nsc.ruБирюковМ. Ю.BiryukovM.

Новосибирск

Novosibirsk

https://orcid.org/0000-0002-3548-4354СухихИ. С.SukhikhI.

Новосибирск

Novosibirsk

ШацкаяН. В.ShatskayaN. V.

Новосибирск

Novosibirsk

ФетВ.FetV.

Отделение биологических наук

Хантингтон

Department of Biological Sciences

Huntington

БлиновА. Г.BlinovA.

Новосибирск

Novosibirsk

КонопацкаяИ. Д.KonopatskaiaI.

Новосибирск

Novosibirsk

Федеральный исследовательский центр Институт цитологии и генетики Сибирского отделения Российской академии наукРоссияInstitute of Cytology and Genetics, SB RASRussian FederationУниверситет МаршаллСоединённые Штаты АмерикиMarshall UniversityUnited StatesФедеральный исследовательский центр Институт цитологии и генетики Сибирского отделения Российской академии наук; Институт молекулярной и клеточной биологии Сибирского отделения Российской академии наукРоссияInstitute of Cytology and Genetics, SB RAS; Institute of Molecular and Cellular Biology, SB RASRussian Federation20190901202023810591066Copyright © Устьянцев К.В., Бирюков М.Ю., Сухих И.С., Шацкая Н.В., Фет В., Блинов А.Г., Конопацкая И.Д., 20202020Устьянцев К.В., Бирюков М.Ю., Сухих И.С., Шацкая Н.В., Фет В., Блинов А.Г., Конопацкая И.Д.Ustyantsev K., Biryukov M., Sukhikh I., Shatskaya N.V., Fet V., Blinov A., Konopatskaia I.This work is licensed under a Creative Commons Attribution 4.0 License.https://vavilov.elpub.ru/jour/article/view/2401

Mariner-подобные элементы (МПЭ) – одни из самых распространенных мобильных элементов эукариот. Насекомые – первые организмы, в которых были обнаружены МПЭ, однако до сих пор еще не изучено разнообразие МПЭ насекомых отряда Orthoptera. В настоящей работе, совмещая результаты биоинформационного анализа секвенированных вырожденных ПЦР-ампликонов МПЭ и доступных геномных последовательностей Orthoptera, мы исследуем разнообразие МПЭ у 16 видов Orthoptera, принадлежащих к трем инфраотрядам: Acrido idea (Caelifera), Grylloidea (Ensifera) и Tettigoniidea (Ensifera). Всего среди всех изученных геномов выявлено 75 генетических линий МПЭ (Ortmar). Автоматизированная филогенетически опосредованная классификация подразделила все известное в настоящее время разнообразие МПЭ на семь кластеров со значимой статистической поддержкой (I–VII), в каждом из которых была найдена одна из генетических линий МПЭ Orthoptera. Большинство генетических линий (36 из 75) принадлежит к кластеру I; 20 линий относятся к кластеру VI; 6, 7, 4 и по 1 линии принадлежат к кластерам II, IV, VII, III и V соответственно. Два кластера, II и IV, были представлены единственными генетическими линиями МПЭ Orthoptera, Ortmar37 и Ortmar45, соответственно, распространенными среди большинства исследованных геномов Orthoptera. Мы также показали, что 16 генетических линий МПЭ Orthoptera могли участвовать в горизонтальном переносе с эволюционно удаленными таксонами насекомых из других отрядов.

Mariner-like elements (MLEs) are among the most widespread DNA transposable elements in eukaryotes. Insects were the first organisms in which MLEs were identified, however the diversity of MLEs in the insect order Orthoptera has not yet been addressed. In the present study, we explore the diversity of MLEs elements in 16 species of Orthoptera belonging to three infraorders, Acridoidea (Caelifera), Grylloidea (Ensifera), and Tettigoniidea (Ensifera) by combining data mined from computational analysis of sequenced degenerative PCR MLE amplicons and available Orthoptera genomic scaffolds. In total, 75 MLE lineages (Ortmar) were identified in all the studied genomes. Automatic phylogeny-based classification suggested that the current known variability of MLEs can be assigned to seven statistically well-supported phylogenetic clusters (I–VII), and the identified Orthoptera lineages were distributed among all of them. The majority of the lineages (36 out of 75) belong to cluster I; 20 belong to cluster VI; and seven, six, four, one and one lineages belong to clusters II, IV, VII, III, and V, respectively. Two of the clusters (II and IV) were composed of a single Orthoptera MLE lineage each (Ortmar37 and Ortmar45, respectively) which were distributed in the vast majority of the studied Orthoptera genomes. Finally, for 16 Orthoptera MLE lineages, horizontal transfer from the distantly related taxa belonging to other insect orders may have occurred. We believe that our study can serve as a basis for future researches on the diversity, distribution, and evolution of MLEs in species of other taxa that are still lacking the sequenced genomes.

mariner-подобные элементымобильные элементыOrthopteraнасекомыегоризонтальный переносmariner-like elementstransposable elementsOrthopteraInsectshorizontal transferThe work of K. Ustyantsev and A. Blinov was funded by the Russian State Budget (Project No. 0324-2019-0040). The work of M. Biryukov was funded by the Russian State Budget (Project No. 0259-2019-0010). We thank Dr. A.G. Bugrov for providing the insect specimens and the Center of Genome Investigation of the Institute of Cytology and Genetics SB RAS for the sequencing assistance.The work of K. Ustyantsev and A. Blinov was funded by the Russian State Budget (Project No. 0324-2019-0040). The work of M. Biryukov was funded by the Russian State Budget (Project No. 0259-2019-0010). We thank Dr. A.G. Bugrov for providing the insect specimens and the Center of Genome Investigation of the Institute of Cytology and Genetics SB RAS for the sequencing assistance.ReferencesAkaike H. 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The authors declare that there are no conflicts of interest present.