vavilovВавиловский журнал генетики и селекцииVavilov Journal of Genetics and Breeding2500-3259Institute of Cytology and Genetics of Siberian Branch of the RAS10.18699/VJ20.595vavilov-2480Research ArticleЭКОЛОГИЧЕСКАЯ ГЕНЕТИКАECOLOGICAL GENETICSВыявление Ehrlichia spp. и Theileria spp. в клещах Hyalomma anatolicum, собранных в ТаджикистанеDetection of Ehrlichia spp. and Theileria spp. in Hyalomma anatolicum ticks collected in Tajikistanhttps://orcid.org/0000-0002-7857-6822КарташовМ. Ю.KartashovM. Yu.

Р.п. Кольцово, Новосибирская область

Koltsovo, Novosibirsk region

https://orcid.org/0000-0002-3677-3668КононоваЮ. В.KononovaYu. V.

Р.п. Кольцово, Новосибирская область

Koltsovo, Novosibirsk region

https://orcid.org/0000-0002-0276-9839ПетроваИ. Д.PetrovaI. D.

Р.п. Кольцово, Новосибирская область

Koltsovo, Novosibirsk region

https://orcid.org/0000-0001-5034-5005ТупотаН. Л.TupotaN. L.

Р.п. Кольцово, Новосибирская область

Koltsovo, Novosibirsk region

https://orcid.org/0000-0003-4350-4260МикрюковаТ. П.MikryukovaT. P.

Р.п. Кольцово, Новосибирская область

Koltsovo, Novosibirsk region

https://orcid.org/0000-0003-1275-171XТерновойВ. А.TernovoiV. A.

Р.п. Кольцово, Новосибирская область

Koltsovo, Novosibirsk region

https://orcid.org/0000-0001-5034-5005ТишковаФ. Х.TishkovaF. H.https://orcid.org/0000-0002-0229-321XЛоктевВ. Б.LoktevV. B.

Р.п. Кольцово, Новосибирская область

Koltsovo, Novosibirsk region

loktev@vector.nsc.ruГосударственный научный центр вирусологии и биотехнологии «Вектор» Роспотребнадзора; Новосибирский национальный исследовательский государственный университетРоссияState Research Center for Virology and Biotechnology “Vector"; Novosibirsk State UniversityRussian FederationГосударственный научный центр вирусологии и биотехнологии «Вектор» Роспотребнадзора; Национальный исследовательский Томский государственный университетРоссияState Research Center for Virology and Biotechnology “Vector"; Tomsk State UniversityRussian FederationГосударственный научный центр вирусологии и биотехнологии «Вектор» РоспотребнадзораРоссияState Research Center for Virology and Biotechnology “Vector"Russian FederationТаджикский научно-исследовательский институт профилактической медициныТаджикистанTajik Research Institute of Preventive MedicineTajikistanГосударственный научный центр вирусологии и биотехнологии «Вектор» Роспотребнадзора; Новосибирский национальный исследовательский государственный университет; Национальный исследовательский Томский государственный университет; Федеральный исследовательский центр Институт цитологии и генетики Сибирского отделения Российской академии наукРоссияState Research Center for Virology and Biotechnology “Vector"; Novosibirsk State University; Tomsk State University; Institute of Cytology and Genetics of Siberian Branch of the Russian Academy of SciencesRussian Federation2020180320202415559Copyright © Карташов М.Ю., Кононова Ю.В., Петрова И.Д., Тупота Н.Л., Микрюкова Т.П., Терновой В.А., Тишкова Ф.Х., Локтев В.Б., 20202020Карташов М.Ю., Кононова Ю.В., Петрова И.Д., Тупота Н.Л., Микрюкова Т.П., Терновой В.А., Тишкова Ф.Х., Локтев В.Б.Kartashov M.Y., Kononova Y.V., Petrova I.D., Tupota N.L., Mikryukova T.P., Ternovoi V.A., Tishkova F.H., Loktev V.B.This work is licensed under a Creative Commons Attribution 4.0 License.https://vavilov.elpub.ru/jour/article/view/2480

Исследовано наличие генетического материала Rickettsiaspp., Ehrlichiaspp., Anaplasmaspp., Babesiaspp. и Theileriaspp. в клещах Hyalommaanatolicum, собранных в юго-западной части Таджикистана, с последующим секвенированием и филогенетическим анализом фрагментов 16S рРНК гена и groESL оперона для Ehrlichiaspp. и фрагмента гена 18S рРНК для Theileriaspp., обнаруженного в изученных клещах. Клещи H. anatolicum были собраны в районах Турсунзаде и Рудаки и исследованы с использованием специфичных праймеров c помощью ПЦР на наличие генетического материала Rickettsiaspp., Ehrlichiaspp., Anaplasmaspp., Babesiaspp. и Theileriaspp. Выделенные ПЦР-фрагменты генов 16S рРНК, groESL оперона и 18S рРНК были секвенированы и проведен их филогенетический анализ с целью генотипирования обнаруженных изо-лятов клещевых патогенов. В клещах, собранных в районе Рудаки, обнаружена ДНК Ehrlichiaspp. (3.3 %) и Theileriaspp. (3.3 %), а в Турсунзаде - ДНК Ehrlichiaspp. (0.7 %). Секвенирование фрагментов гена 16S рРНК и groESL оперона Ehrlichiaspp. показало высокий уровень гомологии нуклеотидной последовательности с известными последовательностями Ehrlichiaspp. Таджикские изоляты Theileriaspp. были генотипированы как Theileriaannulata на основе анализа последовательности гена 18S рРНК. Филогенетический анализ показал, что таджикские изоляты Ehrlichiaspp. очень близки с изолятами Ehrlichiaspp., циркулирующими в Китае и Бразилии. Изолят Tajikistan-5 кластеризуется с предполагаемым новым видом Ehrlichiamineirensis. Таджикские изоляты Theileriaspp. были генетически схожи с вариантами T. annulata, циркулирующими в Турции, Иране, Пакистане и Китае.

The objectives of our study were to survey the prevalence of genetic markers for Rickettsia spp., Ehrlichia spp., Anaplasma spp., Babesia spp., and Theileria spp. in Hyalomma anatolicum ticks collected in southwestern Tajikistan and to perform sequencing and phylogenetic analysis of fragments of the 16S rRNA gene and groESL ope-ron from Ehrlichia spp. and fragments of the 18S rRNA gene of Theileria spp. detected in H. anatolicum ticks. Hyalomma anatolicum ticks collected in the Tursunzade and Rudaki districts of Tajikistan were tested for DNA of Rickettsia spp., Ehrlichia spp., Anaplasma spp., Babesia spp., and Theileria spp. by PCR with specific primers. The amplified fragments were sequenced and analyzed. DNA of Ehrlichia spp. (3.3 %) and Theileria spp. (3.3 %) was detected only in H. anatolicum ticks collected from the Rudaki district, and DNA of Ehrlichia spp. (0.7 %) was found in H. anatolicum ticks from the Tursunzade district. Sequence analysis of fragments of the 16S rRNA gene and groESL operon from Ehrlichia spp. revealed high similarity to Ehrlichia spp. The Tajik isolates of Theileria spp. were genotyped as Theileriaannulata based on the analysis of 18S rRNA gene sequences. The phylogenetic analysis demonstrates that Ehrlichia spp. isolates are highly similar to Ehrlichia spp. circulating in China and Brazil. The isolate Tajikistan-5 is closely related to the putative novel species Ehrlichia mineirensis. The Tajik isolates of Theileria spp. were clustered with T. annulata isolates from Turkey, Iran, Pakistan, and China by phylogenetic analyses.

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The authors declare that there are no conflicts of interest present.