vavilovВавиловский журнал генетики и селекцииVavilov Journal of Genetics and Breeding2500-3259Institute of Cytology and Genetics of Siberian Branch of the RAS10.18699/VJ20.596vavilov-2481Research ArticleЭКОЛОГИЧЕСКАЯ ГЕНЕТИКАECOLOGICAL GENETICSДикий горох (Pisum sativumL. subsp. elatius (Bieb.) Aschers. et Graebn. s.l.) на периферии ареала: горы ЗагросWild pea (Pisum sativumPisum sativum L. subsp. elatius (Bieb.) Aschers. et Graebn. s.l.) at the periphery of its range: Zagros Mountainshttps://orcid.org/0000-0001-5955-4057КостеринО. Э.KosterinO. E.

Новосибирск

Novosibirsk

kosterin@bionet.nsc.ruБогдановаВ. С.BogdanovaV. S.

Новосибирск

Novosibirsk

МглинецА. В.MglinetsA. V.

Новосибирск

Novosibirsk

Федеральный исследовательский центр Институт цитологии и генетики Сибирского отделения, Российская академия наукРоссияInstitute of Cytology and Genetics of Siberian Branch of the Russian Academy of Sciences; Novosibirsk State UniversityRussian FederationФедеральный исследовательский центр Институт цитологии и генетики Сибирского отделения, Российская академия наук; Новосибирский национальный исследовательский государственный университетРоссияInstitute of Cytology and Genetics of Siberian Branch of the Russian Academy of SciencesRussian FederationФедеральный исследовательский центр Институт цитологии и генетики Сибирского отделения, Российская академия наукРоссияInstitute of Cytology and Genetics of Siberian Branch of the Russian Academy of SciencesRussian Federation2020180320202416068Copyright © Костерин О.Э., Богданова В.С., Мглинец А.В., 20202020Костерин О.Э., Богданова В.С., Мглинец А.В.Kosterin O.E., Bogdanova V.S., Mglinets A.V.This work is licensed under a Creative Commons Attribution 4.0 License.https://vavilov.elpub.ru/jour/article/view/2481

Местообитания и признаки диких сородичей гороха на периферии их ареала небезынтересны ввиду их возможного значения для селекции на соответствие различным условиям среды. В то же время разнообразие дикого гороха из периферийных регионов недостаточно представлено в мировых коллекциях генетического материала. На периферии ареала его популяции встречаются редко, невелики по размеру и страдают от землепользования и изменений климата, поэтому весьма желателен их целенаправленный поиск. В ходе двухнедельной экспедиции в Иран в мае 2017 г. были обнаружены две популяции дикого посевного гороха (PisumsativumL. subsp. elatius (Bieb.) Aschers. etGraebn.) в горах Загрос, в районах Алигударз и Хоррамабад провинции Луристан, Иран, на высоте 1841 и 1971 м над уровнем моря соответственно. Приведены краткие описания их местообитаний. Полученные из этих популяций линии гороха, CE9 и CE10, охарактеризованы в отношении некоторых внешних и молекулярных признаков. Они принадлежат к эволюционной линии B, выявленной нами ранее и противопоставленной так называемой линии АС в пределах вида Psativum, имеют уникальную неконсервативную замену в субтипе 5 гистона Н1 и наиболее родственны некоторым образцам из южной и юго-восточной Турции и Голанских высот. Имеющаяся в литературе скудная информация о местонахождениях дикого гороха в Иране свидетельствует, что он распространен в этой стране на юго-западном макросклоне гор Загрос и на южных макросклонах гор Эльбурс и Копетдаг. Обнаружено, что представители эволюционной линии В дикого гороха формируют плохо раскрытые и слабо пигментированные цветки в условиях теплицы, тогда как в природе и сеточных растильнях на открытом воздухе в условиях г. Реховот, Израиль, они производят нормально раскрытые и пигментированные цветки. Обсуждаются некоторые актуальные вопросы таксономии гороха.

Characteristics of wild peas and their habitats at the periphery of the range are interesting with respect to their potential importance for pre-breeding programs aimed at selection for different environmental conditions. However, wild pea diversity in peripheral regions is insufficiently represented in the existing germplasm collections. In such regions, wild pea populations are rare, small in size and suffer from climatic change and land exploitation, hence their focused search is strongly desirable. A two-week-long expedition to Iran in May 2017 revealed two small populations of the wild pea (Pisum sativum subsp. elatius) in the Zagros Mts, in Aligudarz and Khorramabad Districts of Lorestan Province, Iran, at elevations of 1841 and 1971 m a.s.l., respectively. Their habitats are briefly described. Two pea accessions derived from them, CE9 and CE10, were characterised for some visible and molecular characters. These peas appeared to belong to the evolutionary lineage B, recognised by us earlier in P sativum as opposed to the so-called lineage AC. They contain a unique non-conservative substitution in subtype 5 of histone H1 and turned to be most related to some wild pea accessions originating from southern and south-eastern Turkey and Golan Heights. Scarce information available on wild pea occurrence in Iran suggests their existence in the south-western principal slope of Zagros Mts and southern principal slopes of Elborz and Kopet Dagh Mts. It was found that wild peas representing the evolutionary lineage B produce poorly open and poorly coloured flowers (as reported by us earlier) only in the greenhouse conditions but normally pigmented and open flowers in the wild and mesh houses at open air in Israel. Some issues of pea taxonomy are discussed.

Pisum sativum L. subsp. elatius (Bieb.) Aschers. et Graebn.Pisum sativum L. subsp. biflorum (Rafin.) SoldanoLathyrus oleraceus L. subsp. biflorus (Rafin.)Coulot et Rabauteгорохдикие сородичи культурных растенийИрангоры ЗагросПлодородный полумесяцPisum sativum L. subsp. elatius (Bieb.) Aschers. et Graebn.Pisum sativum L. subsp. biflorum (Rafin.) SoldanoLathyrus oleraceus L. subsp. biflorus (Rafin.) Coulot et Rabautepeacrop wild relativesIranZagros MountainsFertile CrescentThe work is supported by Russian State Scientific Project No. 0324-2019-0039-C-01 at the Institute of Cytology & Genetics SB RAS, Novosibirsk, and the project No. 19-04-00162 of the Russian Fund for Fundamental Research. The first author is grateful to Azam Ahmadi and Igor Grichanov for the opportunity to join their expedition to Iran and great help in the field. Shahal Abbo kindly made it possible to take photos of the flowers of CE9 and CE10 grown in a mesh house in Rehovot as well as of wild peas in a natural habitat at Tell Abu Nida Mt., IsraelReferencesAbbo S., Lev-Yadun S., Heun M. Gopher A. On the ‘lost crops’ of the neolithic Near East. J. Exp. Bot. 2013;64:815-822. DOI 10.1093/jxb/ers373.Abbo S., Lev-Yadun S., Heun M. Gopher A. On the ‘lost crops’ of the neolithic Near East. J. Exp. Bot. 2013;64:815-822. DOI 10.1093/jxb/ers373.Abbo S., Zesak I., Schwartz E., Lev-Yadun S., Gopher A. Experimental harvesting of wild peas in Israel: implications for the origins of Near East farming. J. Archaeol. Sci. 2008;35:922-929. DOI 10.1016/j.jas.2007.06.016.Abbo S., Zesak I., Schwartz E., Lev-Yadun S., Gopher A. Experimental harvesting of wild peas in Israel: implications for the origins of Near East farming. J. Archaeol. Sci. 2008;35:922-929. DOI 10.1016/j.jas.2007.06.016.Ben-Ze’ev N., Zohary D. Species relationship in the genus Pisum L. Israel J. Botany. 1973;2:73-91.Ben-Ze’ev N., Zohary D. Species relationship in the genus Pisum L. Israel J. Botany. 1973;2:73-91.Berdnikov V.A., Trusov Y.A., Bogdanova V.S., Kosterin O.E., Ro-zov S.M., Nedel’kina S.V., Nikulina Y.N. The neoplastic pod gene (Np) may be a factor for resistance in pea to the pest Bruchus pisorum L. Pisum Genetics. 1992;24:37-39.Berdnikov V.A., Trusov Y.A., Bogdanova V.S., Kosterin O.E., Ro-zov S.M., Nedel’kina S.V., Nikulina Y.N. The neoplastic pod gene (Np) may be a factor for resistance in pea to the pest Bruchus pisorum L. Pisum Genetics. 1992;24:37-39.Blixt S. Mutation genetics in Pisum. Agri Hortique Genetica. 1972;30: 1-294.Blixt S. Mutation genetics in Pisum. Agri Hortique Genetica. 1972;30: 1-294.Bogdanova V.S., Mglinets A.V., Shatskaya N.V., Kosterin O.E., Solovyev V.I., Vasiliev G.V. Cryptic divergences in the genus Pisum L. (peas), as revealed by phylogenetic analysis of plastid genomes. Mol. Phylogenet. Evol. 2018;129:280-290.Bogdanova V.S., Mglinets A.V., Shatskaya N.V., Kosterin O.E., Solovyev V.I., Vasiliev G.V. Cryptic divergences in the genus Pisum L. (peas), as revealed by phylogenetic analysis of plastid genomes. Mol. Phylogenet. Evol. 2018;129:280-290.Coulot P., Rabaute P. Monographie de Leguminosae de France. 4. Tribus des Fabeae, des Cicereae et des Genisteae. Bulletin de la Societe Botanique du Centre-Ouest. 2016;46:1-902.Coulot P., Rabaute P. Monographie de Leguminosae de France. 4. Tribus des Fabeae, des Cicereae et des Genisteae. Bulletin de la Societe Botanique du Centre-Ouest. 2016;46:1-902.Coulot P., Rabaute P. Deuxiemes complements a la Monographie des Leguminosae de France. Le Monde des Plantes. 2017;516:11-35.Coulot P., Rabaute P. Deuxiemes complements a la Monographie des Leguminosae de France. Le Monde des Plantes. 2017;516:11-35.Coyne C.J., McGee R.J., Redden R.J., Ambrose M.J., Furman B.J., Miles C.A. Genetic adjustment to changing climate: pea. In: Yadav S.S., Redden J.H., Lotze-Campen H., Hall A.J. (Eds.). Crop Adaptation to Climate Change. New York: John Wiley & Sons, 2011;238-250.Coyne C.J., McGee R.J., Redden R.J., Ambrose M.J., Furman B.J., Miles C.A. Genetic adjustment to changing climate: pea. In: Yadav S.S., Redden J.H., Lotze-Campen H., Hall A.J. (Eds.). Crop Adaptation to Climate Change. New York: John Wiley & Sons, 2011;238-250.Grichanov I.Y., Ahmadi A., Kosterin O.E. New records of long-legged flies (Diptera, Dolichopodidae) from Central and North-Eastern Iran. Acta Biologica Sibirica. 2017;3(4):99-112.Grichanov I.Y., Ahmadi A., Kosterin O.E. New records of long-legged flies (Diptera, Dolichopodidae) from Central and North-Eastern Iran. Acta Biologica Sibirica. 2017;3(4):99-112.International Code of Nomenclature for algae, fungi, and plants (Melbourne Code). Oberreifenberg: Koeltz Scientific Books, 2012.International Code of Nomenclature for algae, fungi, and plants (Melbourne Code). Oberreifenberg: Koeltz Scientific Books, 2012.Kosterin O.E. Under the reign of the Pea King (the difficult fate of the first genetical object. Russ. J. Genet.: Appl. Res. 2016a:6(1):1-14. DOI 10.1134/S2079059716010081.Kosterin O.E. Under the reign of the Pea King (the difficult fate of the first genetical object. Russ. J. Genet.: Appl. Res. 2016a:6(1):1-14. DOI 10.1134/S2079059716010081.Kosterin O.E. Prospects of the use of wild relatives for pea breeding. Russ. J. Genet.: Appl. Res. 2016b;6(3):233-243. DOI 10.1134/S2079059716030047.Kosterin O.E. Prospects of the use of wild relatives for pea breeding. Russ. J. Genet.: Appl. Res. 2016b;6(3):233-243. DOI 10.1134/S2079059716030047.Kosterin O.E. Abyssinian pea (Lathyrus schaeferi Kosterin nom. nov. pro Pisum abyssinicum A. Br.) is a problematic taxon. Vavilovskii Zhurnal Genetiki i Selektsii = Vavilov Journal of Genetics and Breeding. 2017;21(2):158-169. DOI 10.18699/VJ17.234. (in Russian).Kosterin O.E. Abyssinian pea (Lathyrus schaeferi Kosterin nom. nov. pro Pisum abyssinicum A. Br.) is a problematic taxon. Vavilovskii Zhurnal Genetiki i Selektsii = Vavilov Journal of Genetics and Breeding. 2017;21(2):158-169. DOI 10.18699/VJ17.234. (in Russian).Kosterin O.E., Ahmadi A. Odonata observed in Central Zagros, Iran, in late May 2017. International Dragonfly Fund Report. 2018;117: 1-65.Kosterin O.E., Ahmadi A. Odonata observed in Central Zagros, Iran, in late May 2017. International Dragonfly Fund Report. 2018;117: 1-65.Kosterin O.E., Bogdanova V.S. Relationship of wild and cultivated forms of Pisum L. as inferred from an analysis of three markers, of the plastid, mitochondrial and nuclear genomes. Genet. Res. Crop Evol. 2008;55:735-755. DOI 10.1007/s10722-007-9281-y.Kosterin O.E., Bogdanova V.S. Relationship of wild and cultivated forms of Pisum L. as inferred from an analysis of three markers, of the plastid, mitochondrial and nuclear genomes. Genet. Res. Crop Evol. 2008;55:735-755. DOI 10.1007/s10722-007-9281-y.Kosterin O.E., Zaytseva O.O., Bogdanova V.S., Ambrose M. New data on three molecular markers from different cellular genomes in Mediterranean accessions reveal new insights into phylogeography of Pisum sativum L. subsp. elatuis (Beib.) Schmahl. Genet. Res. Crop Evol. 2010;57:733-739. DOI 10.1007/s10722-009-9511-6.Kosterin O.E., Zaytseva O.O., Bogdanova V.S., Ambrose M. New data on three molecular markers from different cellular genomes in Mediterranean accessions reveal new insights into phylogeography of Pisum sativum L. subsp. elatuis (Beib.) Schmahl. Genet. Res. Crop Evol. 2010;57:733-739. DOI 10.1007/s10722-009-9511-6.Makasheva R.Kh. Cultivated Flora of the USSR. Vol. IV. Pulse and Legume Cultures. Part I. Pea. Leningrad: Kolos Publ., 1979. (in Russian).Makasheva R.Kh. Cultivated Flora of the USSR. Vol. IV. Pulse and Legume Cultures. Part I. Pea. Leningrad: Kolos Publ., 1979. (in Russian).Maxted N., Ambrose M. Peas (Pisum L.). In: Maxted N., Bennett S.J. (Eds.). Plant Genetic Resources of Legumes in the Mediterranean (Current Plant Science and Biotechnology in Agriculture. 39). Klu-wer Acad. Publ., Dordrecht, 2001;181-190.Maxted N., Ambrose M. Peas (Pisum L.). In: Maxted N., Bennett S.J. (Eds.). Plant Genetic Resources of Legumes in the Mediterranean (Current Plant Science and Biotechnology in Agriculture. 39). Klu-wer Acad. Publ., Dordrecht, 2001;181-190.Maxted N., Kell S.P. Establishment of a global network for the in situ conservation of crop wild relatives: status and needs. FAO Commission on Genetic Resources for Food and Agriculture, Rome, 2009. DOI 10.1023/B:BIOC.0000011719.03230.17.Maxted N., Kell S.P. Establishment of a global network for the in situ conservation of crop wild relatives: status and needs. FAO Commission on Genetic Resources for Food and Agriculture, Rome, 2009. DOI 10.1023/B:BIOC.0000011719.03230.17.Pakravan M., Jalilian N., Neamati M. Flora of Iran, No. 33. Papiliona-ceae (Vicieae). Ministry of Agriculture, Islamic Republic of Iran, Tehran, 2000. (in Farsi)Pakravan M., Jalilian N., Neamati M. Flora of Iran, No. 33. Papiliona-ceae (Vicieae). Ministry of Agriculture, Islamic Republic of Iran, Tehran, 2000. (in Farsi)Reichinger K.H. Pisum. In: Chrtkova-Zertova A., van der Mae-sen L.J.G., Reichinger K.H. Papilionaceae I - Vicieae. Flora des Iranischen Hohlandes und der umrachmenden Gebirge. Persien, Afghanistan, Teile von West-Pakistan, Nord-Iraq, Azerbaijan, Turkmenistan. No. 140. Academische Druck und Verlagsanstalt, Graz, 1979;83-86.Reichinger K.H. Pisum. In: Chrtkova-Zertova A., van der Mae-sen L.J.G., Reichinger K.H. Papilionaceae I - Vicieae. Flora des Iranischen Hohlandes und der umrachmenden Gebirge. Persien, Afghanistan, Teile von West-Pakistan, Nord-Iraq, Azerbaijan, Turkmenistan. No. 140. Academische Druck und Verlagsanstalt, Graz, 1979;83-86.Schaefer H., Hechenleitner P., Santos-Guerra A., Menezes de Sequei-ra M., Pennington R.T., Kenicer G., Carine M.A. Systematics, biogeography, and character evolution of the legume tribe Fabeae with special focus on the middle-Atlantic island lineages. BMC Evol. Biol. 2012;12:250. DOI 10.1186/1471-2148-12-250.Schaefer H., Hechenleitner P., Santos-Guerra A., Menezes de Sequei-ra M., Pennington R.T., Kenicer G., Carine M.A. Systematics, biogeography, and character evolution of the legume tribe Fabeae with special focus on the middle-Atlantic island lineages. BMC Evol. Biol. 2012;12:250. DOI 10.1186/1471-2148-12-250.Soldano A. Riproposizione di taxa sottospecifici prioritari dovuti a bo-tanici italiani. Natura Bresciana (Ann. Mus. Civ. Sci. Nat., Brescia). 1992;27:51-56.Soldano A. Riproposizione di taxa sottospecifici prioritari dovuti a bo-tanici italiani. Natura Bresciana (Ann. Mus. Civ. Sci. Nat., Brescia). 1992;27:51-56.Weiss E., Zohary D. The Neolithic Southwest Asian founder crops, their biology and archaeobotany. Curr. Anthropol. 2011;52(Suppl.4): S237-S254. DOI 10.1086/658367.Weiss E., Zohary D. The Neolithic Southwest Asian founder crops, their biology and archaeobotany. Curr. Anthropol. 2011;52(Suppl.4): S237-S254. DOI 10.1086/658367.Zaytseva O.O., Bogdanova V.S., Kosterin O.E. Phylogenetic reconstruction at the species and intraspecies levels in the genus Pisum (L.) (peas) using a histone H1 gene. Gene. 2012;504:192-202. DOI 10.1016/j.gene.2012.05.026.Zaytseva O.O., Bogdanova V.S., Kosterin O.E. Phylogenetic reconstruction at the species and intraspecies levels in the genus Pisum (L.) (peas) using a histone H1 gene. Gene. 2012;504:192-202. DOI 10.1016/j.gene.2012.05.026.Zaytseva O.O., Bogdanova V.S., Mglinets A.V., Kosterin O.E. Refinement of the collection of wild peas (Pisum L.) and search for the area of pea domestication with a deletion in the plastidic psbA-trnH spacer. Genet. Resour. Crop Evol. 2017;64:1417-1430. DOI 10.1007/s10722-016-0446-4.Zaytseva O.O., Bogdanova V.S., Mglinets A.V., Kosterin O.E. Refinement of the collection of wild peas (Pisum L.) and search for the area of pea domestication with a deletion in the plastidic psbA-trnH spacer. Genet. Resour. Crop Evol. 2017;64:1417-1430. DOI 10.1007/s10722-016-0446-4.Zaytseva O.O., Gunbin K.V., Mglinets A.V., Kosterin O.E. Divergence and population traits in evolution of the genus Pisum L. as reconstructed using genes of two histone H1 subtypes showing different phylogenetic resolution. Gene. 2015;556:235-244.Zaytseva O.O., Gunbin K.V., Mglinets A.V., Kosterin O.E. Divergence and population traits in evolution of the genus Pisum L. as reconstructed using genes of two histone H1 subtypes showing different phylogenetic resolution. Gene. 2015;556:235-244.Zlatcovic B., Mikić A., Smykal P. Distribution and new records of Pisum sativum subsp. elatius in Serbia. Pisum Genetics. 2010;42: 15-17.Zlatcovic B., Mikić A., Smykal P. Distribution and new records of Pisum sativum subsp. elatius in Serbia. Pisum Genetics. 2010;42: 15-17.Zohary D. Geobotanical foundations of the Middle East. I-II. Gustav Fischer Verlag, Stutgart, 1973.Zohary D. Geobotanical foundations of the Middle East. I-II. Gustav Fischer Verlag, Stutgart, 1973.Zohary D., Hopf M. Domestication of Plants in the Old World. 3rd edn. Clarendon Press, Oxford, 2000.Zohary D., Hopf M. Domestication of Plants in the Old World. 3rd edn. Clarendon Press, Oxford, 2000.

The authors declare that there are no conflicts of interest present.