vavilovВавиловский журнал генетики и селекцииVavilov Journal of Genetics and Breeding2500-3259Institute of Cytology and Genetics of Siberian Branch of the RAS10.18699/VJ20.632vavilov-2649Research ArticleБИОТЕХНОЛОГИЯ В ПОСТГЕНОМНУЮ ЭРУMEDICAL GENETICSПолиморфизм и экспрессия генов пролиферации и дифференцировки иммунокомпетентных клеток у лиц, подвергшихся радиационному воздействиюSingle nucleotide polymorphism and expression of genes for immune competent cell proliferation and differentiation in radiation-exposed individualshttps://orcid.org/0000-0002-2567-7945БлиноваЕ. А.BlinovaE. A.

Челябинск

Chelyabinsk

blinova@urcrm.ruhttps://orcid.org/0000-0002-6685-1823НикифоровВ. С.NikiforovV. S.

Челябинск

Chelyabinsk

https://orcid.org/0000-0002-2649-5123ЯнишевскаяМ. А.YanishevskayaM. A.

Челябинск

Chelyabinsk

АклеевА. А.AkleyevA. A.

Челябинск

Chelyabinsk

Уральский научно-практический центр радиационной медицины Федерального медико-биологического агентства России; Челябинский государственный университетРоссияUrals Research Center for Radiation Medicine; Chelyabinsk State UniversityRussian FederationУральский научно-практический центр радиационной медицины Федерального медико-биологического агентства РоссииРоссияUrals Research Center for Radiation MedicineRussian FederationУральский научно-практический центр радиационной медицины Федерального медико-биологического агентства России; Южно-Уральский государственный медицинский университет Министерства здравоохранения Российской ФедерацииРоссияUrals Research Center for Radiation Medicine; South-Urals State Medical University of the Ministry of Healthcare of Russian FederationRussian Federation202002072020244399406Copyright © Блинова Е.А., Никифоров В.С., Янишевская М.А., Аклеев А.А., 20202020Блинова Е.А., Никифоров В.С., Янишевская М.А., Аклеев А.А.Blinova E.A., Nikiforov V.S., Yanishevskaya M.A., Akleyev A.A.This work is licensed under a Creative Commons Attribution 4.0 License.https://vavilov.elpub.ru/jour/article/view/2649

Известно, что ионизирующее излучение влияет на экспрессию генов, выполняющих ключевую роль в механизмах поддержания стабильности клеточного гомеостаза. Как правило, изменение в транскриптоме облученной клетки происходит в первые часы и сутки после радиационного воздействия, что обусловливает ее ранний ответ при повреждении генома. В отдаленном периоде также возможны модуляции в транскрипционной активности генов, приводящие к развитию канцерогенных эффектов облучения. Однако для установления роли экзогенных факторов (ионизирующего излучения) в модификации экспрессии генов клеточного гомеостаза необходимо учитывать и роль эндогенных факторов, способных модифицировать транскрипционную активность генов, что особенно актуально в отдаленном периоде после начала радиационного воздействия. К таким факторам могут относиться полиморфные варианты генов, расположенные в регуляторных областях. Цель настоящего исследования – анализ влияния ионизирующего излучения в отдаленном периоде на содержание мРНК генов STAT3, GATA3, NFkB1, PADI4, регулирующих процессы пролиферации и дифференцировки иммунокомпетентных клеток человека, а также оценка связи аллельных вариаций rs1053023, rs4143094, rs28362491, rs874881 на количество мРНК генов STAT3, GATA3, PADI4, NFkB1. Исследование проведено у лиц, подвергшихся аварийному хроническому радиационному воздействию в результате сбросов радиоактивных отходов в реку Течу. Установлено, что спустя 60 лет после начала радиационного воздействия у лиц, имеющих кумулятивные дозы облучения ККМ в диапазоне от 78 до 3510 мГр, регистрируются изменения в транскрипционной активности генов NFkB1 и PADI4. Не выявлено влияния аллельных вариаций rs1053023, rs4143094, rs28362491, rs874881 на количество мРНК генов STAT3, GATA3, PADI4, NFkB1 у облученных лиц.

It is known that ionizing radiation influences the expression of the genes that play a key role in the mechanisms of maintaining the stability of cellular homeostasis. As a rule, changes in the transcriptome of an exposed cell occur within the first 24 hours following radiation exposure. And it predetermines early response in the case of genome damage. Later on modulations in gene transcription activity are also possible and could result in a carcinogenic effect. However, in order to find the role of exogenous factors (ionizing radiation), it is also necessary to take into account the contribution of endogenous factors that are able to modify gene transcription activity. This is especially important for long after the onset of radiation exposure. Single nucleotide polymorphisms located in regulatory regions of the genes may belong to this group of factors. The objective of the current study was to analyze the influence of ionizing radiation on the transcription activity of the STAT3, GATA3, NFkB1, PADI4 genes, which regulate proliferation and differentiation of immune competent human cells; and to assess the potential influence of single nucleotide polymorphisms located in regulatory regions of the genes on the amount of mRNA. The study involved people who had been chronically exposed due to releases of radioactive waste into the Techa River. It was observed that 60 years after the onset of radiation exposure changes in the transcription activity of the NFkB1 and PADI4 genes were registered in people with cumulative doses to RBM within the range 78–3510 mGy. In people who had been chronically exposed, the effect of allelic variations in rs1053023, rs4143094, rs28362491, rs874881 on the level of mRNAs of the STAT3, GATA3, PADI4, NFkB1 genes has not been established.

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The authors declare that there are no conflicts of interest present.