References

vavilov

Вавиловский журнал генетики и селекции

Vavilov Journal of Genetics and Breeding

2500-3259

Institute of Cytology and Genetics of Siberian Branch of the RAS

10.18699/VJ20.48-o

vavilov-2706

Research Article

ГЕНЕТИЧЕСКИЕ РЕСУРСЫ И БИОКОЛЛЕКЦИИ

PLANT GENETICS

Консенсусная микросателлитная карта ржи с интегрированными EST-SSR маркерами пшеницы

The consensus rye microsatellite map with EST-SSRs transferred from wheat

https://orcid.org/0000-0003-1529-3347

Видакович

Д. О.

Vidakovic

D. O.

https://orcid.org/0000-0002-0292-1693

Перович

Д.

Perovic

https://orcid.org/0000-0001-7275-3878

Семилет

Т. В.

Semilet

T. V.

https://orcid.org/0000-0003-3301-9026

Бернер

А.

Börner

https://orcid.org/0000-0002-8470-8254

Хлесткина

Е. К.

Khlestkina

E. K.

khlest@bionet.nsc.ru

Институт Юлиуса Кюна; Нови-Садский университет, факультет биологии и экологииГерманияJulius Kuehn-Institute (JKI); University of Novi Sad, Department of Biology and EcologyGermany

Институт Юлиуса КюнаГерманияJulius Kuehn-Institute (JKI)Germany

Федеральный исследовательский центр Всероссийский институт генетических ресурсов растений имени Н.И. Вавилова (ВИР)РоссияFederal Research Center the N.I. Vavilov All-Russian Institute of Plant Genetic Resources (VIR)Russian Federation

Институт генетики растений и растениеводства им. ЛейбницаГерманияLeibniz Institute of Plant Genetics and Crop Plant Research (IPK)Germany

Федеральный исследовательский центр Всероссийский институт генетических ресурсов растений имени Н.И. Вавилова (ВИР); Федеральный исследовательский центр Институт цитологии и генетики Сибирского отделения Российской академии наук; Новосибирский национальный исследовательский государственный университетРоссияFederal Research Center the N.I. Vavilov All-Russian Institute of Plant Genetic Resources (VIR); Institute of Cytology and Genetics of Siberian Branch of the Russian Academy of Sciences; Novosibirsk State UniversityRussian Federation

2020

27082020

245459464

2020

Видакович Д.О., Перович Д., Семилет Т.В., Бернер А., Хлесткина Е.К.

Vidakovic D.O., Perovic D., Semilet T.V., Börner A., Khlestkina E.K.

This work is licensed under a Creative Commons Attribution 4.0 License.

https://vavilov.elpub.ru/jour/article/view/2706

Микросателлитные (SSR) маркеры широко используют для картирования генов ржи и анализа транслокационных линий пшеницы и тритикале. SSR-маркеры с известной внутрихромосомной локализацией очень востребованы для картирования экономически значимых генов и QTL-анализа. Одним из источников новых SSR-маркеров у ржи являются микросателлитные маркеры пшеницы. Несмотря на несколько наборов микросателлитных маркеров, доступных у ржи, по-прежнему необходимо расширение списка SSR, сопоставленных с хромосомами ржи, поскольку на некоторых генетических картах количество SSR-маркеров невелико. Цель настоящего исследования состояла в том, чтобы интегрировать EST-SSR пшеницы в существующие генетические карты ржи и построить консенсусную микросателлитную карту ржи. Четыре картирующих популяции ржи (P87/P105, N6/N2, N7/N2 и N7/N6) тестировали с использованием праймеров CFE (EST-SSR). В результате в молекулярно-генетические карты ржи было интегрировано 23 микросателлитных локуса Xcfe: Xcfe023, -136 и -266 на хромосоме 1R, Xcfe006, -067, -175 и -187 на 2R, Xcfe029 и -282 на 3R, Xcfe004, -100, -152, -224 и -260 на 4R, Xcfe037, -208 и -270 на 5R, Xcfe124, -159 и -277 на 6R, Xcfe010, -143 и -228 на 7R. За исключением Xcfe159 и Xcfe224, все картированные локусы Xcfe были обнаружены в ортологичных позициях с учетом множественных транслокаций в ходе эволюции генома ржи по сравнению с пшеницей. Консенсусная карта построена с использованием данных по четырем картирующим популяциям ржи. Она содержит в общей сложности 123 микросателлитных маркера, 12 SNP, 118 RFLP и 2 изоферментных локуса.

Microsatellite (SSR) markers with known precise intrachromosomal locations are widely used for mapping genes in rye and for the investigation of wheat-rye translocation lines and triticale highly demanded for mapping economically important genes and QTL-analysis. One of the sources of novel SSR markers in rye are microsatellites transferable from the wheat genome. Broadening the list of available SSRs in rye mapped to chromosomes is still needed, since some rye chromosome maps still have just a few microsatellite loci mapped. The goal of the current study was to integrate wheat EST-SSRs into the existing rye genetic maps and to construct a consensus rye microsatellite map. Four rye mapping populations (P87/P105, N6/N2, N7/N2 and N7/N6) were tested with CFE (EST-SSRs) primers. A total of 23 Xcfe loci were mapped on rye chromosomes: Xcfe023, -136 and -266 on chromosome 1R, Xcfe006, -067, -175 and -187 on 2R, Xcfe029 and -282 on 3R, Xcfe004, -100, -152, -224 and -260 on 4R, Xcfe037, -208 and -270 on 5R, Xcfe124, -159 and -277 on 6R, Xcfe010, -143 and -228 on 7R. With the exception of Xcfe159 and Xcfe224, all the Xcfe loci mapped were found in orthologous positions considering multiple evolutionary translocations in the rye genome relative to those of common wheat. The consensus map was constructed using mapping data from the four bi-parental populations. It contains a total of 123 microsatellites, 12 SNPs, 118 RFLPs and 2 isozyme loci.

Secale cerealeSSRTriticum aestivumмикросателлитные маркерыгенетические карты

Secale cerealeSSRTriticum aestivummicrosatellite markersgenetic mapping

Ms Tatiana Semilet was supported by the VIR project No. 0481-2019-0001

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The authors declare that there are no conflicts of interest present.