vavilovВавиловский журнал генетики и селекцииVavilov Journal of Genetics and Breeding2500-3259Institute of Cytology and Genetics of Siberian Branch of the RASvavilov-28Research ArticleСтатьиArticlesМОДЕЛЬ ГЕННОЙ СЕТИ РЕГУЛЯЦИИ ВРЕМЕНИ ЦВЕТЕНИЯ У ОЗИМОЙ ПШЕНИЦЫ И ЯЧМЕНЯA GENE REGULATORY NETWORK MODEL FOR VERNALIZATION AND SEASONAL FLOWERING RESPONSE IN WINTER WHEAT AND BARLEYСтепаненкоИ. Л.StepanenkoI. L.stepan@bionet.nsc.ruСмирноваО. Г.SmirnovaO. G.stepan@bionet.nsc.ruТитовИ. И.TitovI. I.stepan@bionet.nsc.ruФедеральное государственное бюджетное научное учреждение «Федеральный исследовательский центр Институт цитологии и генетики Сибирского отделения Российской академии наук», Новосибирск, РоссияРоссияInstitute of Cytology and Genetics, SB RAS, Novosibirsk, RussiaRussian Federation20121012201416199106Copyright © Степаненко И.Л., Смирнова О.Г., Титов И.И., 20142014Степаненко И.Л., Смирнова О.Г., Титов И.И.Stepanenko I.L., Smirnova O.G., Titov I.I.This work is licensed under a Creative Commons Attribution 4.0 License.https://vavilov.elpub.ru/jour/article/view/28

Переход от вегетативной к репродуктивной стадии развития растений зависит от фотопериода и яровизации. Предложена простая логическая модель генной сети, регулирующей время цветения озимой пшеницы. У озимых сортов злаков чувствительность к яровизации контролируется генами VRN1, VRN2 и VRN3. После яровизации продукт гена VRN1 подавляет ген VRN2, кодирующий репрессор цветения. В результате уровень экспрессии VRN3 возрастает и стимулирует дальнейшее увеличение уровня транскрипции гена VRN1. Гены образуют петлю с положительной обратной связью,что усиливает транскрипцию VRN1 до уровня, необходимого для инициации цветения. В условиях длинного дня экспрессию VRN3 усиливают продукты генов PPD1 и CO2, определяющие чувствительность к фотопериоду. Сезонные изменения длины дня через фоторецепторы передаются циркадным часам, которые модулируют время цветения. Данные о генах ячменя и пшеницы, контролирующих чувствительность к яровизации и фотопериоду, интегрированы в генную сеть. С помощью синхронной булевой модели воспроизведена динамика генной сети. Расширенная модель генной сети может быть использована для проверки согласованности экспериментальных данных и выдвижения новых гипотез о взаимодействии генов.

The transition from vegetative to reproductive development in wheat is regulated by seasonal cues, including vernalization and photoperiod. Here we present a simple logical model of the wheat development gene network. Vernalization accelerates flowering in winter cereals. It is regulated mainly by the vernalization genes VRN1, VRN2, and VRN3. After vernalization, VRN1 downregulates the VRN2 flowering repressor, thereby increasing the VRN3 level. The expression of VRN3 promotes further increases in the VRN1 transcription level, generating a positive feedback loop, which enhances VRN1 transcription to a threshold level required to initiate flowering. The products of the PPD1 and CO2 photoperiod genes increase VRN3 expression under long daylight conditions. Seasonal changes in day length are perceived by plant photoreceptors and transmitted to the circadian clock to modulate flowering time. Here we integrate data on vernalization and photoperiod genes in a gene network. Using a synchronous Boolean model, we have simulated the network dynamics. This model can be useful to test the coherence of experimental data and to hypothesize gene interactions that remain to be discovered.

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The authors declare that there are no conflicts of interest present.