References

vavilov

Вавиловский журнал генетики и селекции

Vavilov Journal of Genetics and Breeding

2500-3259

Institute of Cytology and Genetics of Siberian Branch of the RAS

10.18699/VJ20.664

vavilov-2812

Research Article

ГЕНЕТИЧЕСКИЕ РЕСУРСЫ И БИОКОЛЛЕКЦИИ

PLANT GENETICS

Оценка генетического разнообразия с использованием SSR-маркеров в популяциях Agriophyllum squarrosum L. и Agriophyllum minus Fisch. & Mey., собранных на юго-востоке Казахстана

SSR-based evaluation of genetic diversity in populations of Agriophyllum squarrosum L. and Agriophyllum minus Fisch. & Mey. collected in South-East Kazakhstan

https://orcid.org/0000-0001-5987-2952

Гениевская

Ю. А.

Genievskaya

Алматы

Almaty

https://orcid.org/0000-0002-2767-4350

Карелова

Д. С.

Karelova

Алматы

Almaty

https://orcid.org/0000-0002-9748-507X

Абугалиева

С. И.

Abugalieva

Алматы

Almaty

https://orcid.org/0000-0003-4955-0989

Жао

П.

Zhao

Гансу

Gansu

https://orcid.org/0000-0002-3687-4012

Чен

Г.

Chen

Гансу

Gansu

https://orcid.org/0000-0001-8590-1745

Туруспеков

Е. К.

Turuspekov

Алматы

Almaty

yerlant@yahoo.com

Институт биологии и биотехнологии растенийКазахстанLaboratory of molecular genetics, Institute of Plant Biology and BiotechnologyKazakhstan

Институт биологии и биотехнологии растений; Кафедра биоразнообразия и биоресурсов, Казахский национальный университет им. аль-ФарабиКазахстанLaboratory of molecular genetics, Institute of Plant Biology and Biotechnology; Department of biodiversity and bioresources, al-Farabi Kazakh National UniversityKazakhstan

Ключевая лаборатория по физиологии стресса и экологии в холодных и засушливых регионах, Северо-Западный институт экологической среды и ресурсовКитайKey laboratory of stress physiology and ecology in cold and arid regions, Northwest Institute of Eco-Environment and ResourcesChina

2020

05122020

247697704

2020

Гениевская Ю.А., Карелова Д.С., Абугалиева С.И., Жао П., Чен Г., Туруспеков Е.К.

Genievskaya Y., Karelova D., Abugalieva S., Zhao P., Chen G., Turuspekov Y.

This work is licensed under a Creative Commons Attribution 4.0 License.

https://vavilov.elpub.ru/jour/article/view/2812

Разработка информативных полиморфных ДНК-маркеров для малоизученных родов - необходимое и важное условие для эффективного популяционного анализа живых организмов, в том числе играющих важную экологическую роль в суровых климатических условиях, в частности в пустынях и полупустынях. Примерами подобных малоизученных пустынных видов являются Agriophyllum squarrosum L. и Agriophyllum minus Fisch. & Mey. В недавнем проекте по РНК-секвенированию A. squarrosum было найдено большое количество гипотетических SSR (простые повторяющиеся последовательности) маркеров. В настоящей работе в результате скрининга 24 случайно отобранных SSR-маркеров для трех популяций A. squarrosum и одной популяции A. minus обнаружены 11 новых полиморфных SSR-маркеров. Изучение этих 11 SSR-маркеров показало наличие 16 полиморфных локусов для двух видов Agriophyllum, 8 полиморфных локусов среди трех популяций A. squarrosum и 6 полиморфных локусов для популяции A. minus. Статистический анализ выявил высокое межвидовое, но относительно низкое внутривидовое генетическое разнообразие. Филогенетический анализ и анализ структуры популяций позволили обнаружить явное разделение на уровне видов, а также дивергенцию популяции 1 A. squarrosum относительно популяций 2 и 3 данного вида. Таким образом, нами идентифицирован набор новых и информативных SSR-маркеров, подходящих для изучения генетического разнообразия Agriophyllum.

The development of informative polymorphic DNA markers for poorly studied genera is an important step in population analyses of living organisms, including those that play very important ecological roles in harsh environments, such as desert and semi-desert area. Examples of those poorly studied desert species are Agriophyllum squarrosum L. and Agriophyllum minus Fisch. & Mey. However, a recent RNA-sequencing project in A. squarrosum has proposed a large set of hypothetical SSR (simple sequence repeat) markers. In this work, 11 novel polymorphic SSRs were found due to the screening of 24 randomly selected SSRs for three populations of A. squarrosum and one population of A. minus. The analysis of 11 SSRs revealed 16 polymorphic loci in two Agriophyllum species, 8 polymorphic loci within three populations of A. squarrosum, and 6 polymorphic loci in the population of A. minus. Statistical analyses showed high interspecific, but relatively low intraspecific genetic diversity. The phylogenetic clusterization and population structure analysis have demonstrated a clear segregation of A. minus from A. squarrosum, as well as the separation of population 1 from populations 2 and 3 of A. squarrosum. Thus, we identified the set of novel and informative SSR markers suitable for the study of genetic diversity in Agriophyllum.

кумарчик песчаныйкумарчик малыйAgriophyllum squarrosumAgriophyllum minusSSR-маркерыгенетическое разнообразиеструктура популяции

and riceAgriophyllum squarrosumAgriophyllum minusSSR markersgenetic diversitypopulation structure

The research was supported by the project “Key techniques for sand dune stabilization and vegetation recovery in the typical regions of the countries along the Silk Road Economic Belt” (REF: 2016YFE0203400) granted by the Ministry of Science and Technology of China. The study was also supported by the project АР05131621 “Informational system for molecular genetic and botanical documentation of wild flora in Kazakhstan” granted by the Ministry of Education and Science of the Republic of Kazakhstan.

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