References

vavilov

Вавиловский журнал генетики и селекции

Vavilov Journal of Genetics and Breeding

2500-3259

Institute of Cytology and Genetics of Siberian Branch of the RAS

10.18699/VJ20.681

vavilov-2843

Research Article

ГЕНЕТИКА И СЕЛЕКЦИЯ РАСТЕНИЙ НА ИММУНИТЕТ, КАЧЕСТВО, ПРОДУКТИВНОСТЬ И СТРЕССОУСТОЙЧИВОСТЬ

ANIMAL GENETICS AND BREEDING

Гены-кандидаты продуктивности, выявленные при полногеномном поиске ассоциаций с показателями классности у овец породы российский мясной меринос

Candidate genes for productivity identified by genome-wide association study with indicators of class in the Russian meat merino sheep breed

https://orcid.org/0000-0003-4536-1814

Криворучко

А. Ю.

Krivoruchko

A. Y.

https://orcid.org/0000-0003-2730-2482

Яцык

О. А.

Yatsyk

O. A.

malteze@mail.ru

https://orcid.org/0000-0002-2049-842X

Сафарян

Е. Ю.

Safaryan

E. Y.

Всероссийский научно-исследовательский институт овцеводства и козоводства – филиал Северо-Кавказского федерального научного аграрного центраРоссияAll-Russian Research Institute of Sheep and Goat Breeding – Branch of the North Caucasus Federal Agricultural Research CenterRussian Federation

2020

31122020

248836843

2020

Криворучко А.Ю., Яцык О.А., Сафарян Е.Ю.

Krivoruchko A.Y., Yatsyk O.A., Safaryan E.Y.

This work is licensed under a Creative Commons Attribution 4.0 License.

https://vavilov.elpub.ru/jour/article/view/2843

Полногеномный поиск ассоциаций позволяет идентифицировать локусы и отдельные полиморфизмы, связанные с формированием интересующих фенотипов. При полногеномном анализе у овец особо перспективным представляется изучение особей, отличающихся выдающимися показателями продуктивности – выставочных животных, представителей класса «суперэлита». Целью настоящего исследования явилось выявление новых генов-кандидатов хозяйственно ценных признаков на основе поиска однонуклеотидных полиморфизмов, ассоциированных с принадлежностью к различным бонитировочным классам, у баранов породы российский мясной меринос. Генотипирование животных выполнено с использованием ДНК-биочипов Ovine Infinium HD BeadChip (600K), поиск ассоциаций – с использованием программного обеспечения PLINK v. 1.07. Выявлены высокодостоверные ассоциации между принадлежностью животных к различным бонитировочным классам и частотой встречаемости отдельных однонуклеотидных полиморфизмов на хромосомах 2, 6, 10, 13 и 20. Большая часть замен с высокой достоверностью ассоциаций сконцентрирована на хромосоме 10 в области 30859297–31873769. Для поиска генов-кандидатов отобрано 15 полиморфизмов с наибольшей достоверностью ассоциаций (–log10(р) > 9). Определение местоположения анализируемых однонуклеотидных полиморфизмов относительно новейшей аннотации Oar_rambouillet_v1.0 позволило выявить 11 генов-кандидатов, предположительно, связанных с формированием комплекса фенотипических признаков животных выставочной группы: RXFP2, ALOX5AP, MEDAG, OPN5, PRDM5, PTPRT, TRNAS-GGA, EEF1A1, FRY, ZBTB21-like и B3GLCT-like. Перечисленные гены кодируют белки, вовлеченные в контроль клеточного цикла и репликации ДНК, регуляцию пролиферации и апоптоза клеток; участвующие в липидном и углеводном обменах, развитии воспалительного процесса и работе циркадных ритмов. Благодаря этому рассматриваемые гены-кандидаты могут влиять на формирование экстерьерных особенностей и продуктивные качества овец. Однако необходимы дальнейшие исследования, направленные на подтверждение влияния генов и определение точных механизмов этого воздействия на фенотип.

Genome-wide association studies allow identification of loci and polymorphisms associated with the formation of relevant phenotypes. When conducting a full genome analysis of sheep, particularly promising is the study of individuals with outstanding productivity indicators – exhibition animals, representatives of the super-elite class. The aim of this study was to identify new candidate genes for economically valuable traits based on the search for single nucleotide polymorphisms (SNPs) associated with belonging to different evaluation classes in rams of the Russian meat merino breed. Animal genotyping was performed using Ovine Infinium HD BeadChip 600K DNA, association search was performed using PLINK v. 1.07 software. Highly reliable associations were found between animals belonging to different evaluation classes and the frequency of occurrence of individual SNPs on chromosomes 2, 6, 10, 13, and 20. Most of the substitutions with high association reliability are concentrated on chromosome 10 in the region 10: 30859297–31873769. To search for candidate genes, 15 polymorphisms with the highest association reliability were selected (–log10(р) > 9). Determining the location of the analyzed SNPs relative to the latest annotation Oar_rambouillet_v1.0 allowed to identify 11 candidate genes presumably associated with the formation of a complex of phenotypic traits of animals in the exhibition group: RXFP2, ALOX5AP, MEDAG, OPN5, PRDM5, PTPRT, TRNAS-GGA, EEF1A1, FRY, ZBTB21-like, and B3GLCT-like. The listed genes encode proteins involved in the control of the cell cycle and DNA replication, regulation of cell proliferation and apoptosis, lipid and carbohydrate metabolism, the development of the inflammatory process and the work of circadian rhythms. Thus, the candidate genes under consideration can influence the formation of exterior features and productive qualities of sheep. However, further research is needed to confirm the influence of genes and determine the exact mechanisms for implementing this influence on the phenotype.

овцаоднонуклеотидный полиморфизмполногеномный поиск ассоциацийполногеномный анализ ассоциацийген-кандидатроссийский мясной меринос

sheepSNPgenome-wide association studyGWAScandidate geneRussian meat merino

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The authors declare that there are no conflicts of interest present.