vavilovВавиловский журнал генетики и селекцииVavilov Journal of Genetics and Breeding2500-3259Institute of Cytology and Genetics of Siberian Branch of the RASvavilov-293Research ArticleСтатьиArticlesСПАРИВАНИЕ ГОМЕОЛОГИЧНЫХ ХРОМОСОМ У ОТДАЛЕННЫХ АЛЛОГАПЛОИДНЫХ ГИБРИДОВ РОДА SOLANUMINTERGENOMIC CHROMOSOME PAIRING IN ALLOHAPLOID HYBRIDS OF GENUS SOLANUMГавриленкоТ. А.GavrilenkoT. A.tatjana9972@yandex.ruПендиненГ. И.PendinenG. I.tatjana9972@yandex.ruРоккаВ.-М.RokkaV.-M.АнтоноваО. Ю.AntonovaO. Y.tatjana9972@yandex.ruТимеР.ThiemeR.Государственное научное учреждение Всероссийский научно-исследовательский институт растениеводства им. Н.И. Вавилова, Санкт-Петербург, Россия Санкт-Петербургский государственный университет, Санкт-Петербург, РоссияРоссияVavilov Institute of Plant Industry, Saint-Petersburg, Russia Saint-Petersburg State University, Saint-Petersburg, RussiaRussian FederationГосударственное научное учреждение Всероссийский научно-исследовательский институт растениеводства им. Н.И. Вавилова, Санкт-Петербург, РоссияРоссияVavilov Institute of Plant Industry, Saint-Petersburg, RussiaRussian FederationMTT Agrifood Research Finland, Biotechnology and Food Research, Jokioinen, FinlandФинляндияMTT Agrifood Research Finland, Biotechnology and Food Research, Jokioinen, FinlandFinlandJulius Kühn-Institut, Federal Research Centre for Cultivated Plants, Quedlinburg, GermanyГерманияJulius Kühn-Institut, Federal Research Centre for Cultivated Plants, Quedlinburg, GermanyGermany201419012015184/1650659Copyright © Гавриленко Т.А., Пендинен Г.И., Рокка В., Антонова О.Ю., Тиме Р., 20152015Гавриленко Т.А., Пендинен Г.И., Рокка В., Антонова О.Ю., Тиме Р.Gavrilenko T.A., Pendinen G.I., Rokka V., Antonova O.Y., Thieme R.This work is licensed under a Creative Commons Attribution 4.0 License.https://vavilov.elpub.ru/jour/article/view/293

Изучено спаривание хромосом в мейозе уникальных аллогаплоидных гибридов, полученных с использованием методов клеточной и хромосомной инженерии. Использованы две комбинации гибридов: 1) между дигаплоидом культурного картофеля Solanum tuberosum (АА-геном, 2n = 2x = 24) и дикорастущим видом S. еtuberosum (ЕЕ, 2n = 2x = 24); 2) между культурным томатом S. lycopersicum (LL, 2 n = 2x = 24) и S. etuberosum (ЕЕ, 2n = 2x = 24). Хромосомоспецифичные BAC-клоны картофеля и пробы дифференциально меченой тотальной ДНК родительских видов позволили идентифицировать конъюгирующие хромосомы и их геномную принадлежность. У аллогаплоидов S. tuberosum с S. etuberosum геномного состава АЕ обнаружено до 7 межгеномных бивалентов на клетку; хиазмы формируются в дистальном районе длинного плеча каждой хромосомы набора; хиазмы в коротком плече имеют хромосомы 3, 6, 11 и 12. Для андрогенных регенерантов соматических гибридов S. lycopersicum c S. etuberosum характерен в основном унивалентный мейоз; редкие биваленты (от 0 до 2 на клетку) образованы гомеологами хромосом 4 и 6. Обсуждаются перспективы предложенного подхода, основанного на использовании методов соматической гибридизации и in vitro андрогенеза, для изучения спаривания гомеологичных хромосом и разработки стратегии интрогрессивной гибридизации отдаленных видов растений.

Intergenomic chromosome pairing was analyzed in unique allohaploid hybrids, which had been produced through a process of somatic hybridization followed by a haploidization procedure (in vitro androgenesis). Two hybrid combinations were studied: (1) between cultivated potato S. tuberosum (dihaploid, 2n = 2x = 24, AA genome) and wild species S. etuberosum (2n = 2x = 24, EE genome), (2) between cultivated tomato S. lycopersicum (LL, 2n = 2x = 24) and wild species S. etuberosum (2n = 2x = 24, EE). Genome and chromosome composition of allohaploids were identified through FISH with chromosome specific BAC clones and GISH. Up to 7 intergenomic bivalents per cell were observed in allohaploids between S. tuberosum and S. etuberosum (AE); the chiasmata were distributed in the distal regions of long arms of each chromosome and in the short arms of chromosomes 3, 6, 11, 12. Rare bivalents (from 0 to 2) were observed in allohaploids between S. lycopersicum and S. etuberosum (LE); the chiasmata were distributed in the long arms of chromosome 4 and 6. The perspectives of proposed approach are discussed for application of somatic hybridization and chromosome engineering to study the potential of homeologous pairing and the strategy of introgression.

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The authors declare that there are no conflicts of interest present.